Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells
- Autores
- Lezcano, Virginia Alicia; Plotkin, Lilian; Morelli, Susana Ana
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Currently, there is a global trend to use natural bioactive compounds such as phytoestrogens (PEs), present in a wide variety of foods, for their beneficial biological effects demonstrated in vitro and in vivo including antioxidant, anti-inflammatory, anticancer, and antidiabetic activities. PEs are plant-derived non-steroidal compounds that bind to estrogen receptors and have estrogen-like activity. Given that the increase in life expectancy of the population has led to bone health becoming a major concern, in this work we investigated the effects of the PE quercetin (QUE) on the estrogen receptor-positive murine osteoblastic cell line MC3T3-E1. A dose dependent effect of QUE was observed on cell viability after 48 h of exposure, determined by MTS assay; with inhibition of cell viability at 20-100 µM concentrations and no change at lower concentrations. In parallel, by trypan blue assay a significant increase in cell number was obtained at 1 µM QUE. The wound healing assay show that low doses of QUE stimulate osteoblastic cell migration, with a significant closure at 12h, which further increases after 24h of treatment. Cell migration and proliferation are specific cell functions that require cell attachment and spreading. Using a cell adhesion assay we found a 60% increase in cellular adhesion when cells were treated with 1 µM of QUE, and no changes were observed with higher concentrations. QUE is generally considered to have strong antioxidant potency and provides protection against oxidative injury in cultured cells. We found that the pretreatment with 1 or 10 µM of QUE for 48h protects against H2O2-induced toxicity in MC3T3-E1 cells. Altogether, these results indicate that the beneficial effects of QUE on bone formation cells are observed at low doses while high doses of QUE have shown to be deleterious for MC3T3-E1 cells. Furthermore, QUE at high doses increases Erk1/2 and decreases Akt activation, with the consequent increase in the levels of active pro-apoptotic protein BAD, as assessed by Western blot analysis; and blockade of Erk1/2 activity with PD98059 decreases cell death induced by QUE. Based on these findings, we conclude that QUE has positive effects on migration, proliferation, adhesion and antioxidation of osteoblastic cells when it is used at doses lower than 20 µM; and may be consider a potential natural therapeutic alternative for bone healing repair in osteopathologies.
Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Plotkin, Lilian. Indiana University; Estados Unidos
Fil: Morelli, Susana Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
American Society of Bone and Mineral Research
Atlanta
Estados Unidos
American Society for Bone and Mineral Research - Materia
-
PHYTOESTROGEN
QUERCETIN
MC3T3-E1
ANTIOXIDANT - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/236512
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Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cellsLezcano, Virginia AliciaPlotkin, LilianMorelli, Susana AnaPHYTOESTROGENQUERCETINMC3T3-E1ANTIOXIDANThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Currently, there is a global trend to use natural bioactive compounds such as phytoestrogens (PEs), present in a wide variety of foods, for their beneficial biological effects demonstrated in vitro and in vivo including antioxidant, anti-inflammatory, anticancer, and antidiabetic activities. PEs are plant-derived non-steroidal compounds that bind to estrogen receptors and have estrogen-like activity. Given that the increase in life expectancy of the population has led to bone health becoming a major concern, in this work we investigated the effects of the PE quercetin (QUE) on the estrogen receptor-positive murine osteoblastic cell line MC3T3-E1. A dose dependent effect of QUE was observed on cell viability after 48 h of exposure, determined by MTS assay; with inhibition of cell viability at 20-100 µM concentrations and no change at lower concentrations. In parallel, by trypan blue assay a significant increase in cell number was obtained at 1 µM QUE. The wound healing assay show that low doses of QUE stimulate osteoblastic cell migration, with a significant closure at 12h, which further increases after 24h of treatment. Cell migration and proliferation are specific cell functions that require cell attachment and spreading. Using a cell adhesion assay we found a 60% increase in cellular adhesion when cells were treated with 1 µM of QUE, and no changes were observed with higher concentrations. QUE is generally considered to have strong antioxidant potency and provides protection against oxidative injury in cultured cells. We found that the pretreatment with 1 or 10 µM of QUE for 48h protects against H2O2-induced toxicity in MC3T3-E1 cells. Altogether, these results indicate that the beneficial effects of QUE on bone formation cells are observed at low doses while high doses of QUE have shown to be deleterious for MC3T3-E1 cells. Furthermore, QUE at high doses increases Erk1/2 and decreases Akt activation, with the consequent increase in the levels of active pro-apoptotic protein BAD, as assessed by Western blot analysis; and blockade of Erk1/2 activity with PD98059 decreases cell death induced by QUE. Based on these findings, we conclude that QUE has positive effects on migration, proliferation, adhesion and antioxidation of osteoblastic cells when it is used at doses lower than 20 µM; and may be consider a potential natural therapeutic alternative for bone healing repair in osteopathologies.Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Plotkin, Lilian. Indiana University; Estados UnidosFil: Morelli, Susana Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaAmerican Society of Bone and Mineral ResearchAtlantaEstados UnidosAmerican Society for Bone and Mineral ResearchWilley2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/236512Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells; American Society of Bone and Mineral Research; Atlanta; Estados Unidos; 2016; 223-2232473-4039CONICET DigitalCONICETenghttps://www.asbmr.org/meetings/2016-abstractsinfo:eu-repo/semantics/altIdentifier/url/http://www.asbmr.org/Publications/JBMRInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:48Zoai:ri.conicet.gov.ar:11336/236512instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:48.933CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells |
title |
Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells |
spellingShingle |
Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells Lezcano, Virginia Alicia PHYTOESTROGEN QUERCETIN MC3T3-E1 ANTIOXIDANT |
title_short |
Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells |
title_full |
Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells |
title_fullStr |
Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells |
title_full_unstemmed |
Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells |
title_sort |
Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells |
dc.creator.none.fl_str_mv |
Lezcano, Virginia Alicia Plotkin, Lilian Morelli, Susana Ana |
author |
Lezcano, Virginia Alicia |
author_facet |
Lezcano, Virginia Alicia Plotkin, Lilian Morelli, Susana Ana |
author_role |
author |
author2 |
Plotkin, Lilian Morelli, Susana Ana |
author2_role |
author author |
dc.subject.none.fl_str_mv |
PHYTOESTROGEN QUERCETIN MC3T3-E1 ANTIOXIDANT |
topic |
PHYTOESTROGEN QUERCETIN MC3T3-E1 ANTIOXIDANT |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Currently, there is a global trend to use natural bioactive compounds such as phytoestrogens (PEs), present in a wide variety of foods, for their beneficial biological effects demonstrated in vitro and in vivo including antioxidant, anti-inflammatory, anticancer, and antidiabetic activities. PEs are plant-derived non-steroidal compounds that bind to estrogen receptors and have estrogen-like activity. Given that the increase in life expectancy of the population has led to bone health becoming a major concern, in this work we investigated the effects of the PE quercetin (QUE) on the estrogen receptor-positive murine osteoblastic cell line MC3T3-E1. A dose dependent effect of QUE was observed on cell viability after 48 h of exposure, determined by MTS assay; with inhibition of cell viability at 20-100 µM concentrations and no change at lower concentrations. In parallel, by trypan blue assay a significant increase in cell number was obtained at 1 µM QUE. The wound healing assay show that low doses of QUE stimulate osteoblastic cell migration, with a significant closure at 12h, which further increases after 24h of treatment. Cell migration and proliferation are specific cell functions that require cell attachment and spreading. Using a cell adhesion assay we found a 60% increase in cellular adhesion when cells were treated with 1 µM of QUE, and no changes were observed with higher concentrations. QUE is generally considered to have strong antioxidant potency and provides protection against oxidative injury in cultured cells. We found that the pretreatment with 1 or 10 µM of QUE for 48h protects against H2O2-induced toxicity in MC3T3-E1 cells. Altogether, these results indicate that the beneficial effects of QUE on bone formation cells are observed at low doses while high doses of QUE have shown to be deleterious for MC3T3-E1 cells. Furthermore, QUE at high doses increases Erk1/2 and decreases Akt activation, with the consequent increase in the levels of active pro-apoptotic protein BAD, as assessed by Western blot analysis; and blockade of Erk1/2 activity with PD98059 decreases cell death induced by QUE. Based on these findings, we conclude that QUE has positive effects on migration, proliferation, adhesion and antioxidation of osteoblastic cells when it is used at doses lower than 20 µM; and may be consider a potential natural therapeutic alternative for bone healing repair in osteopathologies. Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Fil: Plotkin, Lilian. Indiana University; Estados Unidos Fil: Morelli, Susana Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina American Society of Bone and Mineral Research Atlanta Estados Unidos American Society for Bone and Mineral Research |
description |
Currently, there is a global trend to use natural bioactive compounds such as phytoestrogens (PEs), present in a wide variety of foods, for their beneficial biological effects demonstrated in vitro and in vivo including antioxidant, anti-inflammatory, anticancer, and antidiabetic activities. PEs are plant-derived non-steroidal compounds that bind to estrogen receptors and have estrogen-like activity. Given that the increase in life expectancy of the population has led to bone health becoming a major concern, in this work we investigated the effects of the PE quercetin (QUE) on the estrogen receptor-positive murine osteoblastic cell line MC3T3-E1. A dose dependent effect of QUE was observed on cell viability after 48 h of exposure, determined by MTS assay; with inhibition of cell viability at 20-100 µM concentrations and no change at lower concentrations. In parallel, by trypan blue assay a significant increase in cell number was obtained at 1 µM QUE. The wound healing assay show that low doses of QUE stimulate osteoblastic cell migration, with a significant closure at 12h, which further increases after 24h of treatment. Cell migration and proliferation are specific cell functions that require cell attachment and spreading. Using a cell adhesion assay we found a 60% increase in cellular adhesion when cells were treated with 1 µM of QUE, and no changes were observed with higher concentrations. QUE is generally considered to have strong antioxidant potency and provides protection against oxidative injury in cultured cells. We found that the pretreatment with 1 or 10 µM of QUE for 48h protects against H2O2-induced toxicity in MC3T3-E1 cells. Altogether, these results indicate that the beneficial effects of QUE on bone formation cells are observed at low doses while high doses of QUE have shown to be deleterious for MC3T3-E1 cells. Furthermore, QUE at high doses increases Erk1/2 and decreases Akt activation, with the consequent increase in the levels of active pro-apoptotic protein BAD, as assessed by Western blot analysis; and blockade of Erk1/2 activity with PD98059 decreases cell death induced by QUE. Based on these findings, we conclude that QUE has positive effects on migration, proliferation, adhesion and antioxidation of osteoblastic cells when it is used at doses lower than 20 µM; and may be consider a potential natural therapeutic alternative for bone healing repair in osteopathologies. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/236512 Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells; American Society of Bone and Mineral Research; Atlanta; Estados Unidos; 2016; 223-223 2473-4039 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/236512 |
identifier_str_mv |
Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells; American Society of Bone and Mineral Research; Atlanta; Estados Unidos; 2016; 223-223 2473-4039 CONICET Digital CONICET |
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eng |
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eng |
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https://www.asbmr.org/meetings/2016-abstracts info:eu-repo/semantics/altIdentifier/url/http://www.asbmr.org/Publications/JBMR |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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Internacional |
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Willey |
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Willey |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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