Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells

Autores
Lezcano, Virginia Alicia; Plotkin, Lilian; Morelli, Susana Ana
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Currently, there is a global trend to use natural bioactive compounds such as phytoestrogens (PEs), present in a wide variety of foods, for their beneficial biological effects demonstrated in vitro and in vivo including antioxidant, anti-inflammatory, anticancer, and antidiabetic activities. PEs are plant-derived non-steroidal compounds that bind to estrogen receptors and have estrogen-like activity. Given that the increase in life expectancy of the population has led to bone health becoming a major concern, in this work we investigated the effects of the PE quercetin (QUE) on the estrogen receptor-positive murine osteoblastic cell line MC3T3-E1. A dose dependent effect of QUE was observed on cell viability after 48 h of exposure, determined by MTS assay; with inhibition of cell viability at 20-100 µM concentrations and no change at lower concentrations. In parallel, by trypan blue assay a significant increase in cell number was obtained at 1 µM QUE. The wound healing assay show that low doses of QUE stimulate osteoblastic cell migration, with a significant closure at 12h, which further increases after 24h of treatment. Cell migration and proliferation are specific cell functions that require cell attachment and spreading. Using a cell adhesion assay we found a 60% increase in cellular adhesion when cells were treated with 1 µM of QUE, and no changes were observed with higher concentrations. QUE is generally considered to have strong antioxidant potency and provides protection against oxidative injury in cultured cells. We found that the pretreatment with 1 or 10 µM of QUE for 48h protects against H2O2-induced toxicity in MC3T3-E1 cells. Altogether, these results indicate that the beneficial effects of QUE on bone formation cells are observed at low doses while high doses of QUE have shown to be deleterious for MC3T3-E1 cells. Furthermore, QUE at high doses increases Erk1/2 and decreases Akt activation, with the consequent increase in the levels of active pro-apoptotic protein BAD, as assessed by Western blot analysis; and blockade of Erk1/2 activity with PD98059 decreases cell death induced by QUE. Based on these findings, we conclude that QUE has positive effects on migration, proliferation, adhesion and antioxidation of osteoblastic cells when it is used at doses lower than 20 µM; and may be consider a potential natural therapeutic alternative for bone healing repair in osteopathologies.
Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Plotkin, Lilian. Indiana University; Estados Unidos
Fil: Morelli, Susana Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
American Society of Bone and Mineral Research
Atlanta
Estados Unidos
American Society for Bone and Mineral Research
Materia
PHYTOESTROGEN
QUERCETIN
MC3T3-E1
ANTIOXIDANT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/236512

id CONICETDig_491be5e4821131ca8316eca29f39eea6
oai_identifier_str oai:ri.conicet.gov.ar:11336/236512
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cellsLezcano, Virginia AliciaPlotkin, LilianMorelli, Susana AnaPHYTOESTROGENQUERCETINMC3T3-E1ANTIOXIDANThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Currently, there is a global trend to use natural bioactive compounds such as phytoestrogens (PEs), present in a wide variety of foods, for their beneficial biological effects demonstrated in vitro and in vivo including antioxidant, anti-inflammatory, anticancer, and antidiabetic activities. PEs are plant-derived non-steroidal compounds that bind to estrogen receptors and have estrogen-like activity. Given that the increase in life expectancy of the population has led to bone health becoming a major concern, in this work we investigated the effects of the PE quercetin (QUE) on the estrogen receptor-positive murine osteoblastic cell line MC3T3-E1. A dose dependent effect of QUE was observed on cell viability after 48 h of exposure, determined by MTS assay; with inhibition of cell viability at 20-100 µM concentrations and no change at lower concentrations. In parallel, by trypan blue assay a significant increase in cell number was obtained at 1 µM QUE. The wound healing assay show that low doses of QUE stimulate osteoblastic cell migration, with a significant closure at 12h, which further increases after 24h of treatment. Cell migration and proliferation are specific cell functions that require cell attachment and spreading. Using a cell adhesion assay we found a 60% increase in cellular adhesion when cells were treated with 1 µM of QUE, and no changes were observed with higher concentrations. QUE is generally considered to have strong antioxidant potency and provides protection against oxidative injury in cultured cells. We found that the pretreatment with 1 or 10 µM of QUE for 48h protects against H2O2-induced toxicity in MC3T3-E1 cells. Altogether, these results indicate that the beneficial effects of QUE on bone formation cells are observed at low doses while high doses of QUE have shown to be deleterious for MC3T3-E1 cells. Furthermore, QUE at high doses increases Erk1/2 and decreases Akt activation, with the consequent increase in the levels of active pro-apoptotic protein BAD, as assessed by Western blot analysis; and blockade of Erk1/2 activity with PD98059 decreases cell death induced by QUE. Based on these findings, we conclude that QUE has positive effects on migration, proliferation, adhesion and antioxidation of osteoblastic cells when it is used at doses lower than 20 µM; and may be consider a potential natural therapeutic alternative for bone healing repair in osteopathologies.Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Plotkin, Lilian. Indiana University; Estados UnidosFil: Morelli, Susana Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaAmerican Society of Bone and Mineral ResearchAtlantaEstados UnidosAmerican Society for Bone and Mineral ResearchWilley2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/236512Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells; American Society of Bone and Mineral Research; Atlanta; Estados Unidos; 2016; 223-2232473-4039CONICET DigitalCONICETenghttps://www.asbmr.org/meetings/2016-abstractsinfo:eu-repo/semantics/altIdentifier/url/http://www.asbmr.org/Publications/JBMRInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:48Zoai:ri.conicet.gov.ar:11336/236512instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:48.933CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells
title Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells
spellingShingle Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells
Lezcano, Virginia Alicia
PHYTOESTROGEN
QUERCETIN
MC3T3-E1
ANTIOXIDANT
title_short Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells
title_full Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells
title_fullStr Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells
title_full_unstemmed Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells
title_sort Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells
dc.creator.none.fl_str_mv Lezcano, Virginia Alicia
Plotkin, Lilian
Morelli, Susana Ana
author Lezcano, Virginia Alicia
author_facet Lezcano, Virginia Alicia
Plotkin, Lilian
Morelli, Susana Ana
author_role author
author2 Plotkin, Lilian
Morelli, Susana Ana
author2_role author
author
dc.subject.none.fl_str_mv PHYTOESTROGEN
QUERCETIN
MC3T3-E1
ANTIOXIDANT
topic PHYTOESTROGEN
QUERCETIN
MC3T3-E1
ANTIOXIDANT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Currently, there is a global trend to use natural bioactive compounds such as phytoestrogens (PEs), present in a wide variety of foods, for their beneficial biological effects demonstrated in vitro and in vivo including antioxidant, anti-inflammatory, anticancer, and antidiabetic activities. PEs are plant-derived non-steroidal compounds that bind to estrogen receptors and have estrogen-like activity. Given that the increase in life expectancy of the population has led to bone health becoming a major concern, in this work we investigated the effects of the PE quercetin (QUE) on the estrogen receptor-positive murine osteoblastic cell line MC3T3-E1. A dose dependent effect of QUE was observed on cell viability after 48 h of exposure, determined by MTS assay; with inhibition of cell viability at 20-100 µM concentrations and no change at lower concentrations. In parallel, by trypan blue assay a significant increase in cell number was obtained at 1 µM QUE. The wound healing assay show that low doses of QUE stimulate osteoblastic cell migration, with a significant closure at 12h, which further increases after 24h of treatment. Cell migration and proliferation are specific cell functions that require cell attachment and spreading. Using a cell adhesion assay we found a 60% increase in cellular adhesion when cells were treated with 1 µM of QUE, and no changes were observed with higher concentrations. QUE is generally considered to have strong antioxidant potency and provides protection against oxidative injury in cultured cells. We found that the pretreatment with 1 or 10 µM of QUE for 48h protects against H2O2-induced toxicity in MC3T3-E1 cells. Altogether, these results indicate that the beneficial effects of QUE on bone formation cells are observed at low doses while high doses of QUE have shown to be deleterious for MC3T3-E1 cells. Furthermore, QUE at high doses increases Erk1/2 and decreases Akt activation, with the consequent increase in the levels of active pro-apoptotic protein BAD, as assessed by Western blot analysis; and blockade of Erk1/2 activity with PD98059 decreases cell death induced by QUE. Based on these findings, we conclude that QUE has positive effects on migration, proliferation, adhesion and antioxidation of osteoblastic cells when it is used at doses lower than 20 µM; and may be consider a potential natural therapeutic alternative for bone healing repair in osteopathologies.
Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Plotkin, Lilian. Indiana University; Estados Unidos
Fil: Morelli, Susana Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
American Society of Bone and Mineral Research
Atlanta
Estados Unidos
American Society for Bone and Mineral Research
description Currently, there is a global trend to use natural bioactive compounds such as phytoestrogens (PEs), present in a wide variety of foods, for their beneficial biological effects demonstrated in vitro and in vivo including antioxidant, anti-inflammatory, anticancer, and antidiabetic activities. PEs are plant-derived non-steroidal compounds that bind to estrogen receptors and have estrogen-like activity. Given that the increase in life expectancy of the population has led to bone health becoming a major concern, in this work we investigated the effects of the PE quercetin (QUE) on the estrogen receptor-positive murine osteoblastic cell line MC3T3-E1. A dose dependent effect of QUE was observed on cell viability after 48 h of exposure, determined by MTS assay; with inhibition of cell viability at 20-100 µM concentrations and no change at lower concentrations. In parallel, by trypan blue assay a significant increase in cell number was obtained at 1 µM QUE. The wound healing assay show that low doses of QUE stimulate osteoblastic cell migration, with a significant closure at 12h, which further increases after 24h of treatment. Cell migration and proliferation are specific cell functions that require cell attachment and spreading. Using a cell adhesion assay we found a 60% increase in cellular adhesion when cells were treated with 1 µM of QUE, and no changes were observed with higher concentrations. QUE is generally considered to have strong antioxidant potency and provides protection against oxidative injury in cultured cells. We found that the pretreatment with 1 or 10 µM of QUE for 48h protects against H2O2-induced toxicity in MC3T3-E1 cells. Altogether, these results indicate that the beneficial effects of QUE on bone formation cells are observed at low doses while high doses of QUE have shown to be deleterious for MC3T3-E1 cells. Furthermore, QUE at high doses increases Erk1/2 and decreases Akt activation, with the consequent increase in the levels of active pro-apoptotic protein BAD, as assessed by Western blot analysis; and blockade of Erk1/2 activity with PD98059 decreases cell death induced by QUE. Based on these findings, we conclude that QUE has positive effects on migration, proliferation, adhesion and antioxidation of osteoblastic cells when it is used at doses lower than 20 µM; and may be consider a potential natural therapeutic alternative for bone healing repair in osteopathologies.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/236512
Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells; American Society of Bone and Mineral Research; Atlanta; Estados Unidos; 2016; 223-223
2473-4039
CONICET Digital
CONICET
url http://hdl.handle.net/11336/236512
identifier_str_mv Beneficial effects of low doses of the phytoestrogen quercetin on osteoblastic cells; American Society of Bone and Mineral Research; Atlanta; Estados Unidos; 2016; 223-223
2473-4039
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.asbmr.org/meetings/2016-abstracts
info:eu-repo/semantics/altIdentifier/url/http://www.asbmr.org/Publications/JBMR
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Willey
publisher.none.fl_str_mv Willey
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613432172609536
score 13.070432