Applying nanotechnology to enhance the efficacy of quercetin in breast cancer cells

Autores
Tiburzi, Silvina Mabel; Lezcano, Virginia Alicia; Principe, Gabriel; Montiel Schneider, María Gabriela; Miravalles, Alicia Beatriz; Lassalle, Verónica Leticia; González Pardo, María Verónica
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Quercetin (QUE), a flavonoid abundant in fruits and vegetables, is known for its diverse biological activities, including potential anticancer effects with limited toxicity to normal cells. Despite its promising properties for breast cancer treatment, QUE faces challenges such as low bioavailability and rapid metabolism in vivo. Nanotechnology-based drug carriers have emerged as a potential strategy to address these issues. We previously showed that QUE exerts antiproliferative effects in MCF-7 breast cancer cells, with an estimated IC50 of 75 µM. In this work, we further investigated free QUE mechanism of action in MCF-7 cells. Clonogenic assays indicated that QUE inhibited the colony formation after 48 h of incubation with concentrations below its IC50. In addition, annexin V/PI staining analysis confirmed that QUE (75 µM) increased the apoptotic cell population by 30% compared to the control after 48 h. Furthermore, intracellular oxidant levels were significantly elevated in QUE-treated MCF-7 cells, as measured by the fluorogenic probe 2′,7′-dichlorofluorescin diacetate. Next, QUE was loaded on magnetic iron oxide nanoparticles coated with polyethylene glycol (MAG@PEG), a nanocarrier previously studied and demonstrated to be non-toxic. The incorporation of QUE (MAG@PEG@QUE) was qualitatively confirmed by FTIR spectroscopy, while the loading capacity was determined by UV-visible spectroscopy. Cytotoxic studies performed in MCF-7 cells exposed to MAG@PEG@QUE at a concentration equivalent to free QUE (75 µM, 48 h) showed that MAG@PEG@QUE significantly reduced cell proliferation and viability, accompanied by increased apoptosis. Furthermore, MCF-7 cells incubated with MAG@PEG@QUE exhibited changes in actin cytoskeleton, characteristic of apoptotic cells. TEM images confirmed the apoptosis and revealed the presence of vesicles containing clusters of MAG@PEG@QUE within cell cytoplasm. Targeting of magnetic nanoparticles was achieved in the presence of a static magnetic field, which led to a high intracellular accumulation of magnetic nanoparticles and induced cell death in targeted areas, without affecting adjacent cells. In conclusion, these findings suggest that MAG@PEG@QUE exhibits antitumor effects comparable to free QUE. This nanosystem has the potential to improve the bioavailability and targeted delivery of QUE for breast cancer treatment.
Fil: Tiburzi, Silvina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Principe, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Montiel Schneider, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Miravalles, Alicia Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Córdoba
Argentina
Sociedad Argentina de Bioquímica y Biología Molecular
Materia
PHYTOESTROGEN
NANOCARRIER
CYTOTOXICITY
ANTITUMOR ACTIVITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263817
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Applying nanotechnology to enhance the efficacy of quercetin in breast cancer cellsTiburzi, Silvina MabelLezcano, Virginia AliciaPrincipe, GabrielMontiel Schneider, María GabrielaMiravalles, Alicia BeatrizLassalle, Verónica LeticiaGonzález Pardo, María VerónicaPHYTOESTROGENNANOCARRIERCYTOTOXICITYANTITUMOR ACTIVITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Quercetin (QUE), a flavonoid abundant in fruits and vegetables, is known for its diverse biological activities, including potential anticancer effects with limited toxicity to normal cells. Despite its promising properties for breast cancer treatment, QUE faces challenges such as low bioavailability and rapid metabolism in vivo. Nanotechnology-based drug carriers have emerged as a potential strategy to address these issues. We previously showed that QUE exerts antiproliferative effects in MCF-7 breast cancer cells, with an estimated IC50 of 75 µM. In this work, we further investigated free QUE mechanism of action in MCF-7 cells. Clonogenic assays indicated that QUE inhibited the colony formation after 48 h of incubation with concentrations below its IC50. In addition, annexin V/PI staining analysis confirmed that QUE (75 µM) increased the apoptotic cell population by 30% compared to the control after 48 h. Furthermore, intracellular oxidant levels were significantly elevated in QUE-treated MCF-7 cells, as measured by the fluorogenic probe 2′,7′-dichlorofluorescin diacetate. Next, QUE was loaded on magnetic iron oxide nanoparticles coated with polyethylene glycol (MAG@PEG), a nanocarrier previously studied and demonstrated to be non-toxic. The incorporation of QUE (MAG@PEG@QUE) was qualitatively confirmed by FTIR spectroscopy, while the loading capacity was determined by UV-visible spectroscopy. Cytotoxic studies performed in MCF-7 cells exposed to MAG@PEG@QUE at a concentration equivalent to free QUE (75 µM, 48 h) showed that MAG@PEG@QUE significantly reduced cell proliferation and viability, accompanied by increased apoptosis. Furthermore, MCF-7 cells incubated with MAG@PEG@QUE exhibited changes in actin cytoskeleton, characteristic of apoptotic cells. TEM images confirmed the apoptosis and revealed the presence of vesicles containing clusters of MAG@PEG@QUE within cell cytoplasm. Targeting of magnetic nanoparticles was achieved in the presence of a static magnetic field, which led to a high intracellular accumulation of magnetic nanoparticles and induced cell death in targeted areas, without affecting adjacent cells. In conclusion, these findings suggest that MAG@PEG@QUE exhibits antitumor effects comparable to free QUE. This nanosystem has the potential to improve the bioavailability and targeted delivery of QUE for breast cancer treatment.Fil: Tiburzi, Silvina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Principe, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Montiel Schneider, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Miravalles, Alicia Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaLX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology ResearchCórdobaArgentinaSociedad Argentina de Bioquímica y Biología MolecularSociedad Argentina de Bioquímica y Biología Molecular2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263817Applying nanotechnology to enhance the efficacy of quercetin in breast cancer cells; LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research ; Córdoba; Argentina; 2024; 92 - 92CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/2024/abstracts-EN.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:45Zoai:ri.conicet.gov.ar:11336/263817instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:45.509CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Applying nanotechnology to enhance the efficacy of quercetin in breast cancer cells
title Applying nanotechnology to enhance the efficacy of quercetin in breast cancer cells
spellingShingle Applying nanotechnology to enhance the efficacy of quercetin in breast cancer cells
Tiburzi, Silvina Mabel
PHYTOESTROGEN
NANOCARRIER
CYTOTOXICITY
ANTITUMOR ACTIVITY
title_short Applying nanotechnology to enhance the efficacy of quercetin in breast cancer cells
title_full Applying nanotechnology to enhance the efficacy of quercetin in breast cancer cells
title_fullStr Applying nanotechnology to enhance the efficacy of quercetin in breast cancer cells
title_full_unstemmed Applying nanotechnology to enhance the efficacy of quercetin in breast cancer cells
title_sort Applying nanotechnology to enhance the efficacy of quercetin in breast cancer cells
dc.creator.none.fl_str_mv Tiburzi, Silvina Mabel
Lezcano, Virginia Alicia
Principe, Gabriel
Montiel Schneider, María Gabriela
Miravalles, Alicia Beatriz
Lassalle, Verónica Leticia
González Pardo, María Verónica
author Tiburzi, Silvina Mabel
author_facet Tiburzi, Silvina Mabel
Lezcano, Virginia Alicia
Principe, Gabriel
Montiel Schneider, María Gabriela
Miravalles, Alicia Beatriz
Lassalle, Verónica Leticia
González Pardo, María Verónica
author_role author
author2 Lezcano, Virginia Alicia
Principe, Gabriel
Montiel Schneider, María Gabriela
Miravalles, Alicia Beatriz
Lassalle, Verónica Leticia
González Pardo, María Verónica
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PHYTOESTROGEN
NANOCARRIER
CYTOTOXICITY
ANTITUMOR ACTIVITY
topic PHYTOESTROGEN
NANOCARRIER
CYTOTOXICITY
ANTITUMOR ACTIVITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Quercetin (QUE), a flavonoid abundant in fruits and vegetables, is known for its diverse biological activities, including potential anticancer effects with limited toxicity to normal cells. Despite its promising properties for breast cancer treatment, QUE faces challenges such as low bioavailability and rapid metabolism in vivo. Nanotechnology-based drug carriers have emerged as a potential strategy to address these issues. We previously showed that QUE exerts antiproliferative effects in MCF-7 breast cancer cells, with an estimated IC50 of 75 µM. In this work, we further investigated free QUE mechanism of action in MCF-7 cells. Clonogenic assays indicated that QUE inhibited the colony formation after 48 h of incubation with concentrations below its IC50. In addition, annexin V/PI staining analysis confirmed that QUE (75 µM) increased the apoptotic cell population by 30% compared to the control after 48 h. Furthermore, intracellular oxidant levels were significantly elevated in QUE-treated MCF-7 cells, as measured by the fluorogenic probe 2′,7′-dichlorofluorescin diacetate. Next, QUE was loaded on magnetic iron oxide nanoparticles coated with polyethylene glycol (MAG@PEG), a nanocarrier previously studied and demonstrated to be non-toxic. The incorporation of QUE (MAG@PEG@QUE) was qualitatively confirmed by FTIR spectroscopy, while the loading capacity was determined by UV-visible spectroscopy. Cytotoxic studies performed in MCF-7 cells exposed to MAG@PEG@QUE at a concentration equivalent to free QUE (75 µM, 48 h) showed that MAG@PEG@QUE significantly reduced cell proliferation and viability, accompanied by increased apoptosis. Furthermore, MCF-7 cells incubated with MAG@PEG@QUE exhibited changes in actin cytoskeleton, characteristic of apoptotic cells. TEM images confirmed the apoptosis and revealed the presence of vesicles containing clusters of MAG@PEG@QUE within cell cytoplasm. Targeting of magnetic nanoparticles was achieved in the presence of a static magnetic field, which led to a high intracellular accumulation of magnetic nanoparticles and induced cell death in targeted areas, without affecting adjacent cells. In conclusion, these findings suggest that MAG@PEG@QUE exhibits antitumor effects comparable to free QUE. This nanosystem has the potential to improve the bioavailability and targeted delivery of QUE for breast cancer treatment.
Fil: Tiburzi, Silvina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Principe, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Montiel Schneider, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Miravalles, Alicia Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Córdoba
Argentina
Sociedad Argentina de Bioquímica y Biología Molecular
description Quercetin (QUE), a flavonoid abundant in fruits and vegetables, is known for its diverse biological activities, including potential anticancer effects with limited toxicity to normal cells. Despite its promising properties for breast cancer treatment, QUE faces challenges such as low bioavailability and rapid metabolism in vivo. Nanotechnology-based drug carriers have emerged as a potential strategy to address these issues. We previously showed that QUE exerts antiproliferative effects in MCF-7 breast cancer cells, with an estimated IC50 of 75 µM. In this work, we further investigated free QUE mechanism of action in MCF-7 cells. Clonogenic assays indicated that QUE inhibited the colony formation after 48 h of incubation with concentrations below its IC50. In addition, annexin V/PI staining analysis confirmed that QUE (75 µM) increased the apoptotic cell population by 30% compared to the control after 48 h. Furthermore, intracellular oxidant levels were significantly elevated in QUE-treated MCF-7 cells, as measured by the fluorogenic probe 2′,7′-dichlorofluorescin diacetate. Next, QUE was loaded on magnetic iron oxide nanoparticles coated with polyethylene glycol (MAG@PEG), a nanocarrier previously studied and demonstrated to be non-toxic. The incorporation of QUE (MAG@PEG@QUE) was qualitatively confirmed by FTIR spectroscopy, while the loading capacity was determined by UV-visible spectroscopy. Cytotoxic studies performed in MCF-7 cells exposed to MAG@PEG@QUE at a concentration equivalent to free QUE (75 µM, 48 h) showed that MAG@PEG@QUE significantly reduced cell proliferation and viability, accompanied by increased apoptosis. Furthermore, MCF-7 cells incubated with MAG@PEG@QUE exhibited changes in actin cytoskeleton, characteristic of apoptotic cells. TEM images confirmed the apoptosis and revealed the presence of vesicles containing clusters of MAG@PEG@QUE within cell cytoplasm. Targeting of magnetic nanoparticles was achieved in the presence of a static magnetic field, which led to a high intracellular accumulation of magnetic nanoparticles and induced cell death in targeted areas, without affecting adjacent cells. In conclusion, these findings suggest that MAG@PEG@QUE exhibits antitumor effects comparable to free QUE. This nanosystem has the potential to improve the bioavailability and targeted delivery of QUE for breast cancer treatment.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263817
Applying nanotechnology to enhance the efficacy of quercetin in breast cancer cells; LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research ; Córdoba; Argentina; 2024; 92 - 92
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263817
identifier_str_mv Applying nanotechnology to enhance the efficacy of quercetin in breast cancer cells; LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research ; Córdoba; Argentina; 2024; 92 - 92
CONICET Digital
CONICET
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language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/2024/abstracts-EN.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Bioquímica y Biología Molecular
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