Anti-angiogenic and apoptotic actions of the natural flavonoid quercetin in a cellular model of Kaposi´s sarcoma.

Autores
Principe, Gabriel; Lezcano, Virginia Alicia; Tiburzi, Silvina Mabel; González Pardo, María Verónica
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Quercetin (QUE) is a flavonoid that belongs to the family of phytoestrogen and exhibits anticancer properties in multiple types of solid tumor; nevertheless, its effect on virally oncogenic transformed cells is less studied. The viral G Protein-Coupled Receptor (vGPCR) is one of the molecules from the lytic phase of herpesvirus-8 able to induce cellular modifications through a paracrine oncogenic signaling cascade in Kaposi?s sarcoma. We preliminary showed that QUE exerts antiproliferative effects on endothelial cells that stably express vGPCR. In this work, we further explore the mechanism of QUE-induced cell death in vGPCR cells. First, the IC50 of QUE was calculated by crystal violet technique after the treatment of SVEC and vGPCR cells with different concentrations of QUE (1-100 µM) or vehicle (0.1% DMSO) for 48 h. We found that SVEC cells (IC50= 14.09 µM) were more susceptible to QUE treatment than vGPCR cells (IC50= 30.078 µM). Herein, 30 µM of QUE was selected to further characterize the cell death of vGPCR cells. Cell cycle analysis revealed that QUE increase sub G0 phase and reduce S phase of vGPCR treated with QUE for 24 h presuming an apoptotic event. Annexin V/PI stain and caspase-3 activity confirmed that apoptosis takes place in vGPCR cells after QUE treatment. The vGPCR activates and controls the HIF-1α transcription factor promoting the expression of pro-angiogenic molecules such as VEGF. Consistently, qRT-PCR studies indicated that QUE downregulates the expression of HIF-1α and VEGF mRNA in a concentration dependent manner. In conclusion, our findings from this study suggest that QUE promotes its anticancer effects triggering both, anti-angiogenic and pro-apoptotic, program to induce the cell death of the vGPCR cells.
Fil: Principe, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Tiburzi, Silvina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB)
Mendoza
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Materia
PHYTOESTROGEN
CELL DEATH
KAPOSI’S SARCOMA
SIGNALING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228656
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/228656
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anti-angiogenic and apoptotic actions of the natural flavonoid quercetin in a cellular model of Kaposi´s sarcoma.Principe, GabrielLezcano, Virginia AliciaTiburzi, Silvina MabelGonzález Pardo, María VerónicaPHYTOESTROGENCELL DEATHKAPOSI’S SARCOMASIGNALINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Quercetin (QUE) is a flavonoid that belongs to the family of phytoestrogen and exhibits anticancer properties in multiple types of solid tumor; nevertheless, its effect on virally oncogenic transformed cells is less studied. The viral G Protein-Coupled Receptor (vGPCR) is one of the molecules from the lytic phase of herpesvirus-8 able to induce cellular modifications through a paracrine oncogenic signaling cascade in Kaposi?s sarcoma. We preliminary showed that QUE exerts antiproliferative effects on endothelial cells that stably express vGPCR. In this work, we further explore the mechanism of QUE-induced cell death in vGPCR cells. First, the IC50 of QUE was calculated by crystal violet technique after the treatment of SVEC and vGPCR cells with different concentrations of QUE (1-100 µM) or vehicle (0.1% DMSO) for 48 h. We found that SVEC cells (IC50= 14.09 µM) were more susceptible to QUE treatment than vGPCR cells (IC50= 30.078 µM). Herein, 30 µM of QUE was selected to further characterize the cell death of vGPCR cells. Cell cycle analysis revealed that QUE increase sub G0 phase and reduce S phase of vGPCR treated with QUE for 24 h presuming an apoptotic event. Annexin V/PI stain and caspase-3 activity confirmed that apoptosis takes place in vGPCR cells after QUE treatment. The vGPCR activates and controls the HIF-1α transcription factor promoting the expression of pro-angiogenic molecules such as VEGF. Consistently, qRT-PCR studies indicated that QUE downregulates the expression of HIF-1α and VEGF mRNA in a concentration dependent manner. In conclusion, our findings from this study suggest that QUE promotes its anticancer effects triggering both, anti-angiogenic and pro-apoptotic, program to induce the cell death of the vGPCR cells.Fil: Principe, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Tiburzi, Silvina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaLVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB)MendozaArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularTech Science PressCeccarelli, Eduardo Augusto2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228656Anti-angiogenic and apoptotic actions of the natural flavonoid quercetin in a cellular model of Kaposi´s sarcoma.; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB); Mendoza; Argentina; 2022; 1-20327-9545CONICET DigitalCONICETenghttps://newsite.saib.org.ar/congreso2022/info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v47nSuppl.1/50703Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:43Zoai:ri.conicet.gov.ar:11336/228656instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:44.04CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anti-angiogenic and apoptotic actions of the natural flavonoid quercetin in a cellular model of Kaposi´s sarcoma.
title Anti-angiogenic and apoptotic actions of the natural flavonoid quercetin in a cellular model of Kaposi´s sarcoma.
spellingShingle Anti-angiogenic and apoptotic actions of the natural flavonoid quercetin in a cellular model of Kaposi´s sarcoma.
Principe, Gabriel
PHYTOESTROGEN
CELL DEATH
KAPOSI’S SARCOMA
SIGNALING
title_short Anti-angiogenic and apoptotic actions of the natural flavonoid quercetin in a cellular model of Kaposi´s sarcoma.
title_full Anti-angiogenic and apoptotic actions of the natural flavonoid quercetin in a cellular model of Kaposi´s sarcoma.
title_fullStr Anti-angiogenic and apoptotic actions of the natural flavonoid quercetin in a cellular model of Kaposi´s sarcoma.
title_full_unstemmed Anti-angiogenic and apoptotic actions of the natural flavonoid quercetin in a cellular model of Kaposi´s sarcoma.
title_sort Anti-angiogenic and apoptotic actions of the natural flavonoid quercetin in a cellular model of Kaposi´s sarcoma.
dc.creator.none.fl_str_mv Principe, Gabriel
Lezcano, Virginia Alicia
Tiburzi, Silvina Mabel
González Pardo, María Verónica
author Principe, Gabriel
author_facet Principe, Gabriel
Lezcano, Virginia Alicia
Tiburzi, Silvina Mabel
González Pardo, María Verónica
author_role author
author2 Lezcano, Virginia Alicia
Tiburzi, Silvina Mabel
González Pardo, María Verónica
author2_role author
author
author
dc.contributor.none.fl_str_mv Ceccarelli, Eduardo Augusto
dc.subject.none.fl_str_mv PHYTOESTROGEN
CELL DEATH
KAPOSI’S SARCOMA
SIGNALING
topic PHYTOESTROGEN
CELL DEATH
KAPOSI’S SARCOMA
SIGNALING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Quercetin (QUE) is a flavonoid that belongs to the family of phytoestrogen and exhibits anticancer properties in multiple types of solid tumor; nevertheless, its effect on virally oncogenic transformed cells is less studied. The viral G Protein-Coupled Receptor (vGPCR) is one of the molecules from the lytic phase of herpesvirus-8 able to induce cellular modifications through a paracrine oncogenic signaling cascade in Kaposi?s sarcoma. We preliminary showed that QUE exerts antiproliferative effects on endothelial cells that stably express vGPCR. In this work, we further explore the mechanism of QUE-induced cell death in vGPCR cells. First, the IC50 of QUE was calculated by crystal violet technique after the treatment of SVEC and vGPCR cells with different concentrations of QUE (1-100 µM) or vehicle (0.1% DMSO) for 48 h. We found that SVEC cells (IC50= 14.09 µM) were more susceptible to QUE treatment than vGPCR cells (IC50= 30.078 µM). Herein, 30 µM of QUE was selected to further characterize the cell death of vGPCR cells. Cell cycle analysis revealed that QUE increase sub G0 phase and reduce S phase of vGPCR treated with QUE for 24 h presuming an apoptotic event. Annexin V/PI stain and caspase-3 activity confirmed that apoptosis takes place in vGPCR cells after QUE treatment. The vGPCR activates and controls the HIF-1α transcription factor promoting the expression of pro-angiogenic molecules such as VEGF. Consistently, qRT-PCR studies indicated that QUE downregulates the expression of HIF-1α and VEGF mRNA in a concentration dependent manner. In conclusion, our findings from this study suggest that QUE promotes its anticancer effects triggering both, anti-angiogenic and pro-apoptotic, program to induce the cell death of the vGPCR cells.
Fil: Principe, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Tiburzi, Silvina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB)
Mendoza
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
description Quercetin (QUE) is a flavonoid that belongs to the family of phytoestrogen and exhibits anticancer properties in multiple types of solid tumor; nevertheless, its effect on virally oncogenic transformed cells is less studied. The viral G Protein-Coupled Receptor (vGPCR) is one of the molecules from the lytic phase of herpesvirus-8 able to induce cellular modifications through a paracrine oncogenic signaling cascade in Kaposi?s sarcoma. We preliminary showed that QUE exerts antiproliferative effects on endothelial cells that stably express vGPCR. In this work, we further explore the mechanism of QUE-induced cell death in vGPCR cells. First, the IC50 of QUE was calculated by crystal violet technique after the treatment of SVEC and vGPCR cells with different concentrations of QUE (1-100 µM) or vehicle (0.1% DMSO) for 48 h. We found that SVEC cells (IC50= 14.09 µM) were more susceptible to QUE treatment than vGPCR cells (IC50= 30.078 µM). Herein, 30 µM of QUE was selected to further characterize the cell death of vGPCR cells. Cell cycle analysis revealed that QUE increase sub G0 phase and reduce S phase of vGPCR treated with QUE for 24 h presuming an apoptotic event. Annexin V/PI stain and caspase-3 activity confirmed that apoptosis takes place in vGPCR cells after QUE treatment. The vGPCR activates and controls the HIF-1α transcription factor promoting the expression of pro-angiogenic molecules such as VEGF. Consistently, qRT-PCR studies indicated that QUE downregulates the expression of HIF-1α and VEGF mRNA in a concentration dependent manner. In conclusion, our findings from this study suggest that QUE promotes its anticancer effects triggering both, anti-angiogenic and pro-apoptotic, program to induce the cell death of the vGPCR cells.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228656
Anti-angiogenic and apoptotic actions of the natural flavonoid quercetin in a cellular model of Kaposi´s sarcoma.; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB); Mendoza; Argentina; 2022; 1-2
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228656
identifier_str_mv Anti-angiogenic and apoptotic actions of the natural flavonoid quercetin in a cellular model of Kaposi´s sarcoma.; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB); Mendoza; Argentina; 2022; 1-2
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://newsite.saib.org.ar/congreso2022/
info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v47nSuppl.1/50703
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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