Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanisms

Autores
Marchionatti, Ana M.; Pacciaron, Adriana; Tolosa, Nori Graciela
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Quercetin (QT) could be considered as a potential therapeutic agent for different diseases due to its antioxidant, anti-inflammatory, antiviral and anticancer properties. This study was designed to investigate the ability of QT to protect the chick intestine against menadione (MEN) induced injury in vivo and in vitro. Four-week old chicks (Gallus gallus) were treated i.p. with 2.5 μmol of MEN/kg b.w. or with i.l. 50 μM QT or both. QT protected the intestinal Ca2+ absorption against the inhibition caused by MEN, but QT alone did not modify. Glutathione (GSH) depletion provoked by MEN in chick enterocytes was abolished by QT treatment, whereas QT alone did not modify the intestinal GSH content. The enhancement of GSH peroxidase activity produced by MEN was blocked by QT treatment. In contrast, superoxide dismutase activity remained high after simultaneous treatment of enterocytes with MEN and QT. The flavonol also avoided changes in the mitochondrial membrane permeability (swelling) produced by MEN. The FasL/Fas/caspase-3 pathway was activated by MEN, effect that was abrogated by QT. In conclusion, QT may be useful in preventing inhibition of chick intestinal Ca2+ absorption caused by MEN or other substances that deplete GSH, by blocking the oxidative stress and the FasL/Fas/caspase-3 pathway activation.
Fil: Marchionatti, Ana M.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Pacciaron, Adriana. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina
Fil: Tolosa, Nori Graciela. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Materia
Quercetin
Menadione
Calcium
Intestine
Antioxidant
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11363
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanismsMarchionatti, Ana M.Pacciaron, AdrianaTolosa, Nori GracielaQuercetinMenadioneCalciumIntestineAntioxidanthttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Quercetin (QT) could be considered as a potential therapeutic agent for different diseases due to its antioxidant, anti-inflammatory, antiviral and anticancer properties. This study was designed to investigate the ability of QT to protect the chick intestine against menadione (MEN) induced injury in vivo and in vitro. Four-week old chicks (Gallus gallus) were treated i.p. with 2.5 μmol of MEN/kg b.w. or with i.l. 50 μM QT or both. QT protected the intestinal Ca2+ absorption against the inhibition caused by MEN, but QT alone did not modify. Glutathione (GSH) depletion provoked by MEN in chick enterocytes was abolished by QT treatment, whereas QT alone did not modify the intestinal GSH content. The enhancement of GSH peroxidase activity produced by MEN was blocked by QT treatment. In contrast, superoxide dismutase activity remained high after simultaneous treatment of enterocytes with MEN and QT. The flavonol also avoided changes in the mitochondrial membrane permeability (swelling) produced by MEN. The FasL/Fas/caspase-3 pathway was activated by MEN, effect that was abrogated by QT. In conclusion, QT may be useful in preventing inhibition of chick intestinal Ca2+ absorption caused by MEN or other substances that deplete GSH, by blocking the oxidative stress and the FasL/Fas/caspase-3 pathway activation.Fil: Marchionatti, Ana M.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaFil: Pacciaron, Adriana. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; ArgentinaFil: Tolosa, Nori Graciela. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaElsevier2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11363Marchionatti, Ana M.; Pacciaron, Adriana; Tolosa, Nori Graciela; Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanisms; Elsevier; Comparative Biochemistry And Physiology Part A: Molecular And Integrative Physiology; 164; 1; 1-2013; 215-2201095-6433enginfo:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.cbpa.2012.09.007info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1095643312004709info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:06Zoai:ri.conicet.gov.ar:11336/11363instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:06.987CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanisms
title Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanisms
spellingShingle Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanisms
Marchionatti, Ana M.
Quercetin
Menadione
Calcium
Intestine
Antioxidant
title_short Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanisms
title_full Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanisms
title_fullStr Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanisms
title_full_unstemmed Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanisms
title_sort Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanisms
dc.creator.none.fl_str_mv Marchionatti, Ana M.
Pacciaron, Adriana
Tolosa, Nori Graciela
author Marchionatti, Ana M.
author_facet Marchionatti, Ana M.
Pacciaron, Adriana
Tolosa, Nori Graciela
author_role author
author2 Pacciaron, Adriana
Tolosa, Nori Graciela
author2_role author
author
dc.subject.none.fl_str_mv Quercetin
Menadione
Calcium
Intestine
Antioxidant
topic Quercetin
Menadione
Calcium
Intestine
Antioxidant
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Quercetin (QT) could be considered as a potential therapeutic agent for different diseases due to its antioxidant, anti-inflammatory, antiviral and anticancer properties. This study was designed to investigate the ability of QT to protect the chick intestine against menadione (MEN) induced injury in vivo and in vitro. Four-week old chicks (Gallus gallus) were treated i.p. with 2.5 μmol of MEN/kg b.w. or with i.l. 50 μM QT or both. QT protected the intestinal Ca2+ absorption against the inhibition caused by MEN, but QT alone did not modify. Glutathione (GSH) depletion provoked by MEN in chick enterocytes was abolished by QT treatment, whereas QT alone did not modify the intestinal GSH content. The enhancement of GSH peroxidase activity produced by MEN was blocked by QT treatment. In contrast, superoxide dismutase activity remained high after simultaneous treatment of enterocytes with MEN and QT. The flavonol also avoided changes in the mitochondrial membrane permeability (swelling) produced by MEN. The FasL/Fas/caspase-3 pathway was activated by MEN, effect that was abrogated by QT. In conclusion, QT may be useful in preventing inhibition of chick intestinal Ca2+ absorption caused by MEN or other substances that deplete GSH, by blocking the oxidative stress and the FasL/Fas/caspase-3 pathway activation.
Fil: Marchionatti, Ana M.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Pacciaron, Adriana. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina
Fil: Tolosa, Nori Graciela. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
description Quercetin (QT) could be considered as a potential therapeutic agent for different diseases due to its antioxidant, anti-inflammatory, antiviral and anticancer properties. This study was designed to investigate the ability of QT to protect the chick intestine against menadione (MEN) induced injury in vivo and in vitro. Four-week old chicks (Gallus gallus) were treated i.p. with 2.5 μmol of MEN/kg b.w. or with i.l. 50 μM QT or both. QT protected the intestinal Ca2+ absorption against the inhibition caused by MEN, but QT alone did not modify. Glutathione (GSH) depletion provoked by MEN in chick enterocytes was abolished by QT treatment, whereas QT alone did not modify the intestinal GSH content. The enhancement of GSH peroxidase activity produced by MEN was blocked by QT treatment. In contrast, superoxide dismutase activity remained high after simultaneous treatment of enterocytes with MEN and QT. The flavonol also avoided changes in the mitochondrial membrane permeability (swelling) produced by MEN. The FasL/Fas/caspase-3 pathway was activated by MEN, effect that was abrogated by QT. In conclusion, QT may be useful in preventing inhibition of chick intestinal Ca2+ absorption caused by MEN or other substances that deplete GSH, by blocking the oxidative stress and the FasL/Fas/caspase-3 pathway activation.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11363
Marchionatti, Ana M.; Pacciaron, Adriana; Tolosa, Nori Graciela; Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanisms; Elsevier; Comparative Biochemistry And Physiology Part A: Molecular And Integrative Physiology; 164; 1; 1-2013; 215-220
1095-6433
url http://hdl.handle.net/11336/11363
identifier_str_mv Marchionatti, Ana M.; Pacciaron, Adriana; Tolosa, Nori Graciela; Effects of quercetin and menadione on intestinal calcium absorption and the underlying mechanisms; Elsevier; Comparative Biochemistry And Physiology Part A: Molecular And Integrative Physiology; 164; 1; 1-2013; 215-220
1095-6433
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.cbpa.2012.09.007
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1095643312004709
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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