Anti-inflammatory and wound healing properties of polyphenolic extracts from strawberry and blackberry fruits

Autores
Van de Velde, Franco; Esposito, Debora; Grace, Mary H.; Pirovani, Maria Elida; Lila, Mary Ann
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The polyphenolic profiles by HPLC-TOF-MS of strawberry ‘San Andreas’ and blackberry ‘Black Satin’ crude extracts (CE) were analyzed. Anthocyanin-enriched fractions (AEFs) and proanthocyanidin-enriched fractions (PEFs) were prepared, and all samples were probed for in vitro anti-inflammatory and wound healing effects in a LPS-stimulated RAW 264.7 macrophage model and in a skin fibroblast migration and proliferation assay, respectively. Blackberry samples exhibited higher ROS reduction than strawberry's (up to 50% ROS suppression). Berries CEs exhibited 20% inhibition in Cox-2 gene expression, while AEFs and PEFs were inactive at the same concentration. Strawberry AEF and PEF were more active against IL-1β and IL-6 gene expressions than the similar fractions from blackberry, where PEF was more active than AEF (75% suppression by strawberry PEF). Moreover, berry PEFs were the active polyphenol fraction against iNOS gene expression (50% and 65% gen suppression by strawberry and blackberry PEF, respectively), mirroring results of NO synthesis suppression. The cell migration potential of berry polyphenolics was associated with anthocyanins. AEFs showed fibroblast migration around 50% of that registered for the positive control. Results obtained in this work highlight the anti-inflammatory properties of berry polyphenolics, especially due to proanthocyanidins. Moreover, promising results were obtained about the effects of berry anthocyanins on wound healing.
Fil: Van de Velde, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina
Fil: Esposito, Debora. North Carolina State University; Estados Unidos
Fil: Grace, Mary H.. North Carolina State University; Estados Unidos
Fil: Pirovani, Maria Elida. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina
Fil: Lila, Mary Ann. North Carolina State University; Estados Unidos
Materia
ANTHOCYANINS
CYCLOOXYGENASE-2
FRAGARIA ANANASSA
INTERLEUKIN-6
PROANTHOCYANIDINS
RUBUS FRUTICOSUS
SKIN CELL MIGRATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151715
Acceder
id CONICETDig_470d19e8eab7f6cace54ddfaba31156b
oai_identifier_str oai:ri.conicet.gov.ar:11336/151715
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anti-inflammatory and wound healing properties of polyphenolic extracts from strawberry and blackberry fruitsVan de Velde, FrancoEsposito, DeboraGrace, Mary H.Pirovani, Maria ElidaLila, Mary AnnANTHOCYANINSCYCLOOXYGENASE-2FRAGARIA ANANASSAINTERLEUKIN-6PROANTHOCYANIDINSRUBUS FRUTICOSUSSKIN CELL MIGRATIONhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The polyphenolic profiles by HPLC-TOF-MS of strawberry ‘San Andreas’ and blackberry ‘Black Satin’ crude extracts (CE) were analyzed. Anthocyanin-enriched fractions (AEFs) and proanthocyanidin-enriched fractions (PEFs) were prepared, and all samples were probed for in vitro anti-inflammatory and wound healing effects in a LPS-stimulated RAW 264.7 macrophage model and in a skin fibroblast migration and proliferation assay, respectively. Blackberry samples exhibited higher ROS reduction than strawberry's (up to 50% ROS suppression). Berries CEs exhibited 20% inhibition in Cox-2 gene expression, while AEFs and PEFs were inactive at the same concentration. Strawberry AEF and PEF were more active against IL-1β and IL-6 gene expressions than the similar fractions from blackberry, where PEF was more active than AEF (75% suppression by strawberry PEF). Moreover, berry PEFs were the active polyphenol fraction against iNOS gene expression (50% and 65% gen suppression by strawberry and blackberry PEF, respectively), mirroring results of NO synthesis suppression. The cell migration potential of berry polyphenolics was associated with anthocyanins. AEFs showed fibroblast migration around 50% of that registered for the positive control. Results obtained in this work highlight the anti-inflammatory properties of berry polyphenolics, especially due to proanthocyanidins. Moreover, promising results were obtained about the effects of berry anthocyanins on wound healing.Fil: Van de Velde, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química; ArgentinaFil: Esposito, Debora. North Carolina State University; Estados UnidosFil: Grace, Mary H.. North Carolina State University; Estados UnidosFil: Pirovani, Maria Elida. Universidad Nacional del Litoral. Facultad de Ingeniería Química; ArgentinaFil: Lila, Mary Ann. North Carolina State University; Estados UnidosElsevier Science2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151715Van de Velde, Franco; Esposito, Debora; Grace, Mary H.; Pirovani, Maria Elida; Lila, Mary Ann; Anti-inflammatory and wound healing properties of polyphenolic extracts from strawberry and blackberry fruits; Elsevier Science; Food Research International; 121; 7-2019; 453-4620963-9969CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S096399691830944X?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodres.2018.11.059info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:38:22Zoai:ri.conicet.gov.ar:11336/151715instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:38:22.836CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anti-inflammatory and wound healing properties of polyphenolic extracts from strawberry and blackberry fruits
title Anti-inflammatory and wound healing properties of polyphenolic extracts from strawberry and blackberry fruits
spellingShingle Anti-inflammatory and wound healing properties of polyphenolic extracts from strawberry and blackberry fruits
Van de Velde, Franco
ANTHOCYANINS
CYCLOOXYGENASE-2
FRAGARIA ANANASSA
INTERLEUKIN-6
PROANTHOCYANIDINS
RUBUS FRUTICOSUS
SKIN CELL MIGRATION
title_short Anti-inflammatory and wound healing properties of polyphenolic extracts from strawberry and blackberry fruits
title_full Anti-inflammatory and wound healing properties of polyphenolic extracts from strawberry and blackberry fruits
title_fullStr Anti-inflammatory and wound healing properties of polyphenolic extracts from strawberry and blackberry fruits
title_full_unstemmed Anti-inflammatory and wound healing properties of polyphenolic extracts from strawberry and blackberry fruits
title_sort Anti-inflammatory and wound healing properties of polyphenolic extracts from strawberry and blackberry fruits
dc.creator.none.fl_str_mv Van de Velde, Franco
Esposito, Debora
Grace, Mary H.
Pirovani, Maria Elida
Lila, Mary Ann
author Van de Velde, Franco
author_facet Van de Velde, Franco
Esposito, Debora
Grace, Mary H.
Pirovani, Maria Elida
Lila, Mary Ann
author_role author
author2 Esposito, Debora
Grace, Mary H.
Pirovani, Maria Elida
Lila, Mary Ann
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANTHOCYANINS
CYCLOOXYGENASE-2
FRAGARIA ANANASSA
INTERLEUKIN-6
PROANTHOCYANIDINS
RUBUS FRUTICOSUS
SKIN CELL MIGRATION
topic ANTHOCYANINS
CYCLOOXYGENASE-2
FRAGARIA ANANASSA
INTERLEUKIN-6
PROANTHOCYANIDINS
RUBUS FRUTICOSUS
SKIN CELL MIGRATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The polyphenolic profiles by HPLC-TOF-MS of strawberry ‘San Andreas’ and blackberry ‘Black Satin’ crude extracts (CE) were analyzed. Anthocyanin-enriched fractions (AEFs) and proanthocyanidin-enriched fractions (PEFs) were prepared, and all samples were probed for in vitro anti-inflammatory and wound healing effects in a LPS-stimulated RAW 264.7 macrophage model and in a skin fibroblast migration and proliferation assay, respectively. Blackberry samples exhibited higher ROS reduction than strawberry's (up to 50% ROS suppression). Berries CEs exhibited 20% inhibition in Cox-2 gene expression, while AEFs and PEFs were inactive at the same concentration. Strawberry AEF and PEF were more active against IL-1β and IL-6 gene expressions than the similar fractions from blackberry, where PEF was more active than AEF (75% suppression by strawberry PEF). Moreover, berry PEFs were the active polyphenol fraction against iNOS gene expression (50% and 65% gen suppression by strawberry and blackberry PEF, respectively), mirroring results of NO synthesis suppression. The cell migration potential of berry polyphenolics was associated with anthocyanins. AEFs showed fibroblast migration around 50% of that registered for the positive control. Results obtained in this work highlight the anti-inflammatory properties of berry polyphenolics, especially due to proanthocyanidins. Moreover, promising results were obtained about the effects of berry anthocyanins on wound healing.
Fil: Van de Velde, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina
Fil: Esposito, Debora. North Carolina State University; Estados Unidos
Fil: Grace, Mary H.. North Carolina State University; Estados Unidos
Fil: Pirovani, Maria Elida. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina
Fil: Lila, Mary Ann. North Carolina State University; Estados Unidos
description The polyphenolic profiles by HPLC-TOF-MS of strawberry ‘San Andreas’ and blackberry ‘Black Satin’ crude extracts (CE) were analyzed. Anthocyanin-enriched fractions (AEFs) and proanthocyanidin-enriched fractions (PEFs) were prepared, and all samples were probed for in vitro anti-inflammatory and wound healing effects in a LPS-stimulated RAW 264.7 macrophage model and in a skin fibroblast migration and proliferation assay, respectively. Blackberry samples exhibited higher ROS reduction than strawberry's (up to 50% ROS suppression). Berries CEs exhibited 20% inhibition in Cox-2 gene expression, while AEFs and PEFs were inactive at the same concentration. Strawberry AEF and PEF were more active against IL-1β and IL-6 gene expressions than the similar fractions from blackberry, where PEF was more active than AEF (75% suppression by strawberry PEF). Moreover, berry PEFs were the active polyphenol fraction against iNOS gene expression (50% and 65% gen suppression by strawberry and blackberry PEF, respectively), mirroring results of NO synthesis suppression. The cell migration potential of berry polyphenolics was associated with anthocyanins. AEFs showed fibroblast migration around 50% of that registered for the positive control. Results obtained in this work highlight the anti-inflammatory properties of berry polyphenolics, especially due to proanthocyanidins. Moreover, promising results were obtained about the effects of berry anthocyanins on wound healing.
publishDate 2019
dc.date.none.fl_str_mv 2019-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151715
Van de Velde, Franco; Esposito, Debora; Grace, Mary H.; Pirovani, Maria Elida; Lila, Mary Ann; Anti-inflammatory and wound healing properties of polyphenolic extracts from strawberry and blackberry fruits; Elsevier Science; Food Research International; 121; 7-2019; 453-462
0963-9969
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151715
identifier_str_mv Van de Velde, Franco; Esposito, Debora; Grace, Mary H.; Pirovani, Maria Elida; Lila, Mary Ann; Anti-inflammatory and wound healing properties of polyphenolic extracts from strawberry and blackberry fruits; Elsevier Science; Food Research International; 121; 7-2019; 453-462
0963-9969
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S096399691830944X?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodres.2018.11.059
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24909

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