Development of solid phase extraction strategies to minimize the effect of human urine matrix effect on the response of carnitine by UPLC–MS/MS

Autores
Isaguirre, Andrea Celeste; Olsina, Roberto Antonio; Martinez, Luis Dante; Lapierre, Alicia V.; Cerutti, Estela Soledad
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A rapid, selective and sensitive ultra-high performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed to determine carnitine in human urine. Solid phase extraction approaches based on the use of polymeric and weak cationic exchange cartridges were evaluated and applied to the treatment of urine samples. After optimizing the various stages of SPE, a satisfactory set up for retaining substances interfering on carnitine's response was achieved for both types of cartridges. The UPLC separation was carried out on a reversed phase column. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. Residual matrix components were specific to urine samples and interfered on the carnitine signal (a response suppression of 50% was observed). It was then demonstrated that sample treatment by SPE could reduce the effect of the above mentioned interferents, without needing a preliminary derivatization step. The recovery percentage of carnitine obtained after the application of SPE was of approximately 83 ± 7% and, consequently, the matrix effect was minimized. Thus, a sensitive, precise and reliable methodology was developed to determine traces of carnitine in biological fluids.
Fil: Isaguirre, Andrea Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Lapierre, Alicia V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
CARNITINE
SPE
UPLC-(+)ESI–MS/MS
URINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55703
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/55703
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of solid phase extraction strategies to minimize the effect of human urine matrix effect on the response of carnitine by UPLC–MS/MSIsaguirre, Andrea CelesteOlsina, Roberto AntonioMartinez, Luis DanteLapierre, Alicia V.Cerutti, Estela SoledadCARNITINESPEUPLC-(+)ESI–MS/MSURINEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A rapid, selective and sensitive ultra-high performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed to determine carnitine in human urine. Solid phase extraction approaches based on the use of polymeric and weak cationic exchange cartridges were evaluated and applied to the treatment of urine samples. After optimizing the various stages of SPE, a satisfactory set up for retaining substances interfering on carnitine's response was achieved for both types of cartridges. The UPLC separation was carried out on a reversed phase column. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. Residual matrix components were specific to urine samples and interfered on the carnitine signal (a response suppression of 50% was observed). It was then demonstrated that sample treatment by SPE could reduce the effect of the above mentioned interferents, without needing a preliminary derivatization step. The recovery percentage of carnitine obtained after the application of SPE was of approximately 83 ± 7% and, consequently, the matrix effect was minimized. Thus, a sensitive, precise and reliable methodology was developed to determine traces of carnitine in biological fluids.Fil: Isaguirre, Andrea Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Lapierre, Alicia V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier Science2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55703Isaguirre, Andrea Celeste; Olsina, Roberto Antonio; Martinez, Luis Dante; Lapierre, Alicia V.; Cerutti, Estela Soledad; Development of solid phase extraction strategies to minimize the effect of human urine matrix effect on the response of carnitine by UPLC–MS/MS; Elsevier Science; Microchemical Journal; 129; 11-2016; 362-3670026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2016.07.018info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X16301990info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:05Zoai:ri.conicet.gov.ar:11336/55703instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:06.089CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of solid phase extraction strategies to minimize the effect of human urine matrix effect on the response of carnitine by UPLC–MS/MS
title Development of solid phase extraction strategies to minimize the effect of human urine matrix effect on the response of carnitine by UPLC–MS/MS
spellingShingle Development of solid phase extraction strategies to minimize the effect of human urine matrix effect on the response of carnitine by UPLC–MS/MS
Isaguirre, Andrea Celeste
CARNITINE
SPE
UPLC-(+)ESI–MS/MS
URINE
title_short Development of solid phase extraction strategies to minimize the effect of human urine matrix effect on the response of carnitine by UPLC–MS/MS
title_full Development of solid phase extraction strategies to minimize the effect of human urine matrix effect on the response of carnitine by UPLC–MS/MS
title_fullStr Development of solid phase extraction strategies to minimize the effect of human urine matrix effect on the response of carnitine by UPLC–MS/MS
title_full_unstemmed Development of solid phase extraction strategies to minimize the effect of human urine matrix effect on the response of carnitine by UPLC–MS/MS
title_sort Development of solid phase extraction strategies to minimize the effect of human urine matrix effect on the response of carnitine by UPLC–MS/MS
dc.creator.none.fl_str_mv Isaguirre, Andrea Celeste
Olsina, Roberto Antonio
Martinez, Luis Dante
Lapierre, Alicia V.
Cerutti, Estela Soledad
author Isaguirre, Andrea Celeste
author_facet Isaguirre, Andrea Celeste
Olsina, Roberto Antonio
Martinez, Luis Dante
Lapierre, Alicia V.
Cerutti, Estela Soledad
author_role author
author2 Olsina, Roberto Antonio
Martinez, Luis Dante
Lapierre, Alicia V.
Cerutti, Estela Soledad
author2_role author
author
author
author
dc.subject.none.fl_str_mv CARNITINE
SPE
UPLC-(+)ESI–MS/MS
URINE
topic CARNITINE
SPE
UPLC-(+)ESI–MS/MS
URINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A rapid, selective and sensitive ultra-high performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed to determine carnitine in human urine. Solid phase extraction approaches based on the use of polymeric and weak cationic exchange cartridges were evaluated and applied to the treatment of urine samples. After optimizing the various stages of SPE, a satisfactory set up for retaining substances interfering on carnitine's response was achieved for both types of cartridges. The UPLC separation was carried out on a reversed phase column. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. Residual matrix components were specific to urine samples and interfered on the carnitine signal (a response suppression of 50% was observed). It was then demonstrated that sample treatment by SPE could reduce the effect of the above mentioned interferents, without needing a preliminary derivatization step. The recovery percentage of carnitine obtained after the application of SPE was of approximately 83 ± 7% and, consequently, the matrix effect was minimized. Thus, a sensitive, precise and reliable methodology was developed to determine traces of carnitine in biological fluids.
Fil: Isaguirre, Andrea Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Lapierre, Alicia V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description A rapid, selective and sensitive ultra-high performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed to determine carnitine in human urine. Solid phase extraction approaches based on the use of polymeric and weak cationic exchange cartridges were evaluated and applied to the treatment of urine samples. After optimizing the various stages of SPE, a satisfactory set up for retaining substances interfering on carnitine's response was achieved for both types of cartridges. The UPLC separation was carried out on a reversed phase column. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. Residual matrix components were specific to urine samples and interfered on the carnitine signal (a response suppression of 50% was observed). It was then demonstrated that sample treatment by SPE could reduce the effect of the above mentioned interferents, without needing a preliminary derivatization step. The recovery percentage of carnitine obtained after the application of SPE was of approximately 83 ± 7% and, consequently, the matrix effect was minimized. Thus, a sensitive, precise and reliable methodology was developed to determine traces of carnitine in biological fluids.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55703
Isaguirre, Andrea Celeste; Olsina, Roberto Antonio; Martinez, Luis Dante; Lapierre, Alicia V.; Cerutti, Estela Soledad; Development of solid phase extraction strategies to minimize the effect of human urine matrix effect on the response of carnitine by UPLC–MS/MS; Elsevier Science; Microchemical Journal; 129; 11-2016; 362-367
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55703
identifier_str_mv Isaguirre, Andrea Celeste; Olsina, Roberto Antonio; Martinez, Luis Dante; Lapierre, Alicia V.; Cerutti, Estela Soledad; Development of solid phase extraction strategies to minimize the effect of human urine matrix effect on the response of carnitine by UPLC–MS/MS; Elsevier Science; Microchemical Journal; 129; 11-2016; 362-367
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2016.07.018
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X16301990
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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