LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat

Autores
Lattanzio, Verónica M. T.; Solfrizzo, Michele; de Girolamo, Annalisa; Chulze, Sofia Noemi; Torres, Adriana Mabel; Visconti, Angelo
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The understanding of mycotoxins transfer to biological fluids is challenged by the difficulties in performing and replicating in vivo experiments as well as the lack of suitable methods of analysis to detect simultaneously a range of chemically different metabolites at trace levels. LC-MS/MS has been used herein to study the urinary excretion profile of the mycotoxin deoxynivalenol in human and Wistar rat. Deoxynivalenol and deoxynivalenol glucuronide were found in both human and rat urines, whereas de-epoxydeoxynivalenol and its glucuronide conjugate were only detected in rat urine. The presence of two deoxynivalenol glucuronide isomers in Wistar rat urine has been shown for the first time. Structure confirmation of the detected metabolites was provided by the analysis of fragmentation patterns. A solid phase extraction clean up procedure allowing recoveries in the range 72-102% for deoxynivalenol, de-epoxydeoxynivalenol, and their glucuronide conjugates was optimized. A multiple reaction monitoring method for the simultaneous determination of all investigated metabolites was elaborated allowing the direct detection of deoxynivalenol metabolites without the hydrolysis step. Deoxynivalenol urinary levels in the range 0.003-0.008. μg/ml were detected in healthy human subjects, whereas deoxynivalenol and de-epoxynivalenol levels between 1.9-4.9. μg/ml and 1.6-5.9. μg/ml, respectively were found in administered rat urine. These findings emphasize the relevance of the highly selective and sensitive LC-MS/MS technique for the direct detection and characterization of deoxynivalenol metabolites in complex biological matrices. © 2011 Elsevier B.V.
Fil: Lattanzio, Verónica M. T.. National Research Council. Institute of Sciences of Food Production; Italia
Fil: Solfrizzo, Michele. National Research Council. Institute of Sciences of Food Production; Italia
Fil: de Girolamo, Annalisa. National Research Council. Institute of Sciences of Food Production; Italia
Fil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Torres, Adriana Mabel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina
Fil: Visconti, Angelo. National Research Council. Institute of Sciences of Food Production; Italia
Materia
DE-EPOXYDEOXYNIVALENOL
DEOXYNIVALENOL
GLUCURONIDE
HUMAN URINE
LC-MS/MS
RAT URINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/190627
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and ratLattanzio, Verónica M. T.Solfrizzo, Michelede Girolamo, AnnalisaChulze, Sofia NoemiTorres, Adriana MabelVisconti, AngeloDE-EPOXYDEOXYNIVALENOLDEOXYNIVALENOLGLUCURONIDEHUMAN URINELC-MS/MSRAT URINEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The understanding of mycotoxins transfer to biological fluids is challenged by the difficulties in performing and replicating in vivo experiments as well as the lack of suitable methods of analysis to detect simultaneously a range of chemically different metabolites at trace levels. LC-MS/MS has been used herein to study the urinary excretion profile of the mycotoxin deoxynivalenol in human and Wistar rat. Deoxynivalenol and deoxynivalenol glucuronide were found in both human and rat urines, whereas de-epoxydeoxynivalenol and its glucuronide conjugate were only detected in rat urine. The presence of two deoxynivalenol glucuronide isomers in Wistar rat urine has been shown for the first time. Structure confirmation of the detected metabolites was provided by the analysis of fragmentation patterns. A solid phase extraction clean up procedure allowing recoveries in the range 72-102% for deoxynivalenol, de-epoxydeoxynivalenol, and their glucuronide conjugates was optimized. A multiple reaction monitoring method for the simultaneous determination of all investigated metabolites was elaborated allowing the direct detection of deoxynivalenol metabolites without the hydrolysis step. Deoxynivalenol urinary levels in the range 0.003-0.008. μg/ml were detected in healthy human subjects, whereas deoxynivalenol and de-epoxynivalenol levels between 1.9-4.9. μg/ml and 1.6-5.9. μg/ml, respectively were found in administered rat urine. These findings emphasize the relevance of the highly selective and sensitive LC-MS/MS technique for the direct detection and characterization of deoxynivalenol metabolites in complex biological matrices. © 2011 Elsevier B.V.Fil: Lattanzio, Verónica M. T.. National Research Council. Institute of Sciences of Food Production; ItaliaFil: Solfrizzo, Michele. National Research Council. Institute of Sciences of Food Production; ItaliaFil: de Girolamo, Annalisa. National Research Council. Institute of Sciences of Food Production; ItaliaFil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Torres, Adriana Mabel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; ArgentinaFil: Visconti, Angelo. National Research Council. Institute of Sciences of Food Production; ItaliaElsevier Science2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/190627Lattanzio, Verónica M. T.; Solfrizzo, Michele; de Girolamo, Annalisa; Chulze, Sofia Noemi; Torres, Adriana Mabel; et al.; LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat; Elsevier Science; Journal of Chromatography B; 879; 11-12; 4-2011; 707-7150378-43471570-0232CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jchromb.2011.01.029info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1570023211000638info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:36Zoai:ri.conicet.gov.ar:11336/190627instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:36.766CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat
title LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat
spellingShingle LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat
Lattanzio, Verónica M. T.
DE-EPOXYDEOXYNIVALENOL
DEOXYNIVALENOL
GLUCURONIDE
HUMAN URINE
LC-MS/MS
RAT URINE
title_short LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat
title_full LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat
title_fullStr LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat
title_full_unstemmed LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat
title_sort LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat
dc.creator.none.fl_str_mv Lattanzio, Verónica M. T.
Solfrizzo, Michele
de Girolamo, Annalisa
Chulze, Sofia Noemi
Torres, Adriana Mabel
Visconti, Angelo
author Lattanzio, Verónica M. T.
author_facet Lattanzio, Verónica M. T.
Solfrizzo, Michele
de Girolamo, Annalisa
Chulze, Sofia Noemi
Torres, Adriana Mabel
Visconti, Angelo
author_role author
author2 Solfrizzo, Michele
de Girolamo, Annalisa
Chulze, Sofia Noemi
Torres, Adriana Mabel
Visconti, Angelo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv DE-EPOXYDEOXYNIVALENOL
DEOXYNIVALENOL
GLUCURONIDE
HUMAN URINE
LC-MS/MS
RAT URINE
topic DE-EPOXYDEOXYNIVALENOL
DEOXYNIVALENOL
GLUCURONIDE
HUMAN URINE
LC-MS/MS
RAT URINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The understanding of mycotoxins transfer to biological fluids is challenged by the difficulties in performing and replicating in vivo experiments as well as the lack of suitable methods of analysis to detect simultaneously a range of chemically different metabolites at trace levels. LC-MS/MS has been used herein to study the urinary excretion profile of the mycotoxin deoxynivalenol in human and Wistar rat. Deoxynivalenol and deoxynivalenol glucuronide were found in both human and rat urines, whereas de-epoxydeoxynivalenol and its glucuronide conjugate were only detected in rat urine. The presence of two deoxynivalenol glucuronide isomers in Wistar rat urine has been shown for the first time. Structure confirmation of the detected metabolites was provided by the analysis of fragmentation patterns. A solid phase extraction clean up procedure allowing recoveries in the range 72-102% for deoxynivalenol, de-epoxydeoxynivalenol, and their glucuronide conjugates was optimized. A multiple reaction monitoring method for the simultaneous determination of all investigated metabolites was elaborated allowing the direct detection of deoxynivalenol metabolites without the hydrolysis step. Deoxynivalenol urinary levels in the range 0.003-0.008. μg/ml were detected in healthy human subjects, whereas deoxynivalenol and de-epoxynivalenol levels between 1.9-4.9. μg/ml and 1.6-5.9. μg/ml, respectively were found in administered rat urine. These findings emphasize the relevance of the highly selective and sensitive LC-MS/MS technique for the direct detection and characterization of deoxynivalenol metabolites in complex biological matrices. © 2011 Elsevier B.V.
Fil: Lattanzio, Verónica M. T.. National Research Council. Institute of Sciences of Food Production; Italia
Fil: Solfrizzo, Michele. National Research Council. Institute of Sciences of Food Production; Italia
Fil: de Girolamo, Annalisa. National Research Council. Institute of Sciences of Food Production; Italia
Fil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Torres, Adriana Mabel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina
Fil: Visconti, Angelo. National Research Council. Institute of Sciences of Food Production; Italia
description The understanding of mycotoxins transfer to biological fluids is challenged by the difficulties in performing and replicating in vivo experiments as well as the lack of suitable methods of analysis to detect simultaneously a range of chemically different metabolites at trace levels. LC-MS/MS has been used herein to study the urinary excretion profile of the mycotoxin deoxynivalenol in human and Wistar rat. Deoxynivalenol and deoxynivalenol glucuronide were found in both human and rat urines, whereas de-epoxydeoxynivalenol and its glucuronide conjugate were only detected in rat urine. The presence of two deoxynivalenol glucuronide isomers in Wistar rat urine has been shown for the first time. Structure confirmation of the detected metabolites was provided by the analysis of fragmentation patterns. A solid phase extraction clean up procedure allowing recoveries in the range 72-102% for deoxynivalenol, de-epoxydeoxynivalenol, and their glucuronide conjugates was optimized. A multiple reaction monitoring method for the simultaneous determination of all investigated metabolites was elaborated allowing the direct detection of deoxynivalenol metabolites without the hydrolysis step. Deoxynivalenol urinary levels in the range 0.003-0.008. μg/ml were detected in healthy human subjects, whereas deoxynivalenol and de-epoxynivalenol levels between 1.9-4.9. μg/ml and 1.6-5.9. μg/ml, respectively were found in administered rat urine. These findings emphasize the relevance of the highly selective and sensitive LC-MS/MS technique for the direct detection and characterization of deoxynivalenol metabolites in complex biological matrices. © 2011 Elsevier B.V.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/190627
Lattanzio, Verónica M. T.; Solfrizzo, Michele; de Girolamo, Annalisa; Chulze, Sofia Noemi; Torres, Adriana Mabel; et al.; LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat; Elsevier Science; Journal of Chromatography B; 879; 11-12; 4-2011; 707-715
0378-4347
1570-0232
CONICET Digital
CONICET
url http://hdl.handle.net/11336/190627
identifier_str_mv Lattanzio, Verónica M. T.; Solfrizzo, Michele; de Girolamo, Annalisa; Chulze, Sofia Noemi; Torres, Adriana Mabel; et al.; LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat; Elsevier Science; Journal of Chromatography B; 879; 11-12; 4-2011; 707-715
0378-4347
1570-0232
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jchromb.2011.01.029
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1570023211000638
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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