Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MS

Autores
Torres, Alberto Sabier; Cerutti, Estela Soledad; Raba, Julio; Pacheco, Pablo Hugo; Silva, Maria Fernanda
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study describes a method for seleno-amino acids determination in Argentinean olive oils. Preliminary total selenium determination in olive oils probed low concentrations (62.8±1.6 to 117.4±3.0 μg/kg) and the necessity of implementing a preconcentration method. To this end a XAD® resin was employed as sorbent for selenomethionine (Se-Met), selenomethylselenocysteine (Se-MetSeCys), and selenocysteine (Se-Cys) preconcentration. Determinations were performed by UPLC-ESI-MS/MS. Recoveries were between 84% and 97% for the seleno-amino acids studied, reaching a detection limit of 0.09 μg/kg, a precision of 10% (RSD, n=6), and an enhancement factor of 60-fold (6 for the extraction system and 10 for the preconcentration approach). The only detected Se species in the olive oils was Se-MetSeCys in concentrations ranging from 2.0 to 8.3 μg/kg.
Fil: Torres, Alberto Sabier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Pacheco, Pablo Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
Preconcentration
Seleno-Amino Acids
Olive Oils
Spe Uplc-Esi-Ms/Ms
Xad Resin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5748
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/5748
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MSTorres, Alberto SabierCerutti, Estela SoledadRaba, JulioPacheco, Pablo HugoSilva, Maria FernandaPreconcentrationSeleno-Amino AcidsOlive OilsSpe Uplc-Esi-Ms/MsXad Resinhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This study describes a method for seleno-amino acids determination in Argentinean olive oils. Preliminary total selenium determination in olive oils probed low concentrations (62.8±1.6 to 117.4±3.0 μg/kg) and the necessity of implementing a preconcentration method. To this end a XAD® resin was employed as sorbent for selenomethionine (Se-Met), selenomethylselenocysteine (Se-MetSeCys), and selenocysteine (Se-Cys) preconcentration. Determinations were performed by UPLC-ESI-MS/MS. Recoveries were between 84% and 97% for the seleno-amino acids studied, reaching a detection limit of 0.09 μg/kg, a precision of 10% (RSD, n=6), and an enhancement factor of 60-fold (6 for the extraction system and 10 for the preconcentration approach). The only detected Se species in the olive oils was Se-MetSeCys in concentrations ranging from 2.0 to 8.3 μg/kg.Fil: Torres, Alberto Sabier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Pacheco, Pablo Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; ArgentinaElsevier2014-03-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5748Torres, Alberto Sabier; Cerutti, Estela Soledad; Raba, Julio; Pacheco, Pablo Hugo; Silva, Maria Fernanda; Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MS; Elsevier; Food Chemistry; 159; 18-3-2014; 407-4130308-8146enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2014.03.045info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0308814614004361info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:32:34Zoai:ri.conicet.gov.ar:11336/5748instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:32:34.732CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MS
title Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MS
spellingShingle Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MS
Torres, Alberto Sabier
Preconcentration
Seleno-Amino Acids
Olive Oils
Spe Uplc-Esi-Ms/Ms
Xad Resin
title_short Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MS
title_full Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MS
title_fullStr Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MS
title_full_unstemmed Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MS
title_sort Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MS
dc.creator.none.fl_str_mv Torres, Alberto Sabier
Cerutti, Estela Soledad
Raba, Julio
Pacheco, Pablo Hugo
Silva, Maria Fernanda
author Torres, Alberto Sabier
author_facet Torres, Alberto Sabier
Cerutti, Estela Soledad
Raba, Julio
Pacheco, Pablo Hugo
Silva, Maria Fernanda
author_role author
author2 Cerutti, Estela Soledad
Raba, Julio
Pacheco, Pablo Hugo
Silva, Maria Fernanda
author2_role author
author
author
author
dc.subject.none.fl_str_mv Preconcentration
Seleno-Amino Acids
Olive Oils
Spe Uplc-Esi-Ms/Ms
Xad Resin
topic Preconcentration
Seleno-Amino Acids
Olive Oils
Spe Uplc-Esi-Ms/Ms
Xad Resin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study describes a method for seleno-amino acids determination in Argentinean olive oils. Preliminary total selenium determination in olive oils probed low concentrations (62.8±1.6 to 117.4±3.0 μg/kg) and the necessity of implementing a preconcentration method. To this end a XAD® resin was employed as sorbent for selenomethionine (Se-Met), selenomethylselenocysteine (Se-MetSeCys), and selenocysteine (Se-Cys) preconcentration. Determinations were performed by UPLC-ESI-MS/MS. Recoveries were between 84% and 97% for the seleno-amino acids studied, reaching a detection limit of 0.09 μg/kg, a precision of 10% (RSD, n=6), and an enhancement factor of 60-fold (6 for the extraction system and 10 for the preconcentration approach). The only detected Se species in the olive oils was Se-MetSeCys in concentrations ranging from 2.0 to 8.3 μg/kg.
Fil: Torres, Alberto Sabier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Pacheco, Pablo Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina
description This study describes a method for seleno-amino acids determination in Argentinean olive oils. Preliminary total selenium determination in olive oils probed low concentrations (62.8±1.6 to 117.4±3.0 μg/kg) and the necessity of implementing a preconcentration method. To this end a XAD® resin was employed as sorbent for selenomethionine (Se-Met), selenomethylselenocysteine (Se-MetSeCys), and selenocysteine (Se-Cys) preconcentration. Determinations were performed by UPLC-ESI-MS/MS. Recoveries were between 84% and 97% for the seleno-amino acids studied, reaching a detection limit of 0.09 μg/kg, a precision of 10% (RSD, n=6), and an enhancement factor of 60-fold (6 for the extraction system and 10 for the preconcentration approach). The only detected Se species in the olive oils was Se-MetSeCys in concentrations ranging from 2.0 to 8.3 μg/kg.
publishDate 2014
dc.date.none.fl_str_mv 2014-03-18
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5748
Torres, Alberto Sabier; Cerutti, Estela Soledad; Raba, Julio; Pacheco, Pablo Hugo; Silva, Maria Fernanda; Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MS; Elsevier; Food Chemistry; 159; 18-3-2014; 407-413
0308-8146
url http://hdl.handle.net/11336/5748
identifier_str_mv Torres, Alberto Sabier; Cerutti, Estela Soledad; Raba, Julio; Pacheco, Pablo Hugo; Silva, Maria Fernanda; Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MS; Elsevier; Food Chemistry; 159; 18-3-2014; 407-413
0308-8146
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2014.03.045
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0308814614004361
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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