Comparative mapping in the Fagaceae and beyond with EST-SSRs

• Autores: Bodénès, Catherine; Chancerel, Emilie; Gailing, Oliver; Vendramin, Giovanni G.; Bagnoli, Francesca; Durand, Jerome; Goicoechea, Pablo G.; Soliani, Carolina; Villani, Fiorella; Mattioni, Claudia; Koelewijn, Hans Peter; Murat, Florent; Salse, Jerome; Roussel, Guy; Boury, Christophe; Alberto, Florian; Kremer, Antoine; Plomion, Christophe
• Año de publicación: 2012
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Quercus; genetic linkage mapping; EST-SSRs; bud burst
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Background: Genetic markers and linkage mapping are basic prerequisites for comparative genetic analyses, QTL detection and map-based cloning. A large number of mapping populations have been developed for oak, but few gene-based markers are available for constructing integrated genetic linkage maps and comparing gene order and QTL location across related species.Results: We developed a set of 573 expressed sequence tag-derived simple sequence repeats (EST-SSRs) and located 397 markers (EST-SSRs and genomic SSRs) on the 12 oak chromosomes (2n=2x=24) on the basis of Mendelian segregation patterns in 5 full-sib mapping pedigrees of two species: Quercus robur (pedunculate oak) and Quercus petraea (sessile oak). Consensus maps for the two species were constructed and aligned. They showed a high degree of macrosynteny between these two sympatric European oaks. We assessed the transferability ofEST-SSRs to other Fagaceae genera and a subset of these markers was mapped in Castanea sativa, the European chestnut. Reasonably high levels of macrosynteny were observed between oak and chestnut. We also obtained diversity statistics for a subset of EST-SSRs, to support further population genetic analyses with gene-based markers.Finally, based on the orthologous relationships between the oak, Arabidopsis, grape, poplar, Medicago, and soybean genomes and the paralogous relationships between the 12 oak chromosomes, we propose an evolutionary scenario of the 12 oak chromosomes from the eudicot ancestral karyotype.Conclusions: This study provides map locations for a large set of EST-SSRs in two oak species of recognized biological importance in natural ecosystems. This first step toward the construction of a gene-based linkage map will facilitate the assignment of future genome scaffolds to pseudo-chromosomes. This study also provides an indication of the potential utility of new gene-based markers for population genetics and comparative mapping within and beyond the Fagaceae.
Fil: Bodénès, Catherine. Université de Bordeaux; Francia
Fil: Chancerel, Emilie. Université de Bordeaux; Francia
Fil: Gailing, Oliver. Michigan Technological University; Estados Unidos
Fil: Vendramin, Giovanni G.. Plant Genetics Institute; Italia
Fil: Bagnoli, Francesca. Plant Genetics Institute; Italia
Fil: Durand, Jerome. Université de Bordeaux; Francia
Fil: Goicoechea, Pablo G.. No especifíca;
Fil: Soliani, Carolina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Grupo de Genética Forestal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: Villani, Fiorella. No especifíca;
Fil: Mattioni, Claudia. No especifíca;
Fil: Koelewijn, Hans Peter. No especifíca;
Fil: Murat, Florent. No especifíca;
Fil: Salse, Jerome. No especifíca;
Fil: Roussel, Guy. Université de Bordeaux; Francia
Fil: Boury, Christophe. Université de Bordeaux; Francia
Fil: Alberto, Florian. Université de Bordeaux; Francia
Fil: Kremer, Antoine. Université de Bordeaux; Francia
Fil: Plomion, Christophe. Université de Bordeaux; Francia