Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in...

Autores
Siena, Lorena Adelina; Sartor, Maria Esperanza; Quarin, Camilo Luis; Espinoza, Francisco; Ortiz, Juan Pablo Amelio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genus Paspalum includes numerous species of agronomic importance. The objectives of this work were to evaluate the transferability and polymorphism of publicly available wheat EST-SSR (wEST-SSR) markers to Paspalum spp., assess the cross-species amplification of Paspalum notatum genomic-SSRs (PnSSR) within the genus, and evaluate both types of markers for phylogenetic analyses and mapping. Thirty two accessions, including 11 species were used. Moreover, 65 F1 hybrids of P. notatum were employed for mapping. Transferability ratio of wEST-SSRs was 72.72%. On average 19.25 bands per primer combination were obtained. Cross-species amplification of PnSSRs was 55.37%, with an average of 9.45 fragments per primer pair. Both types of markers differed in the amplification capacity between primers pairs and species. Clustering analysis with wEST-SSRs data discriminated accessions by species and taxonomic groups. Genomic relationships were in agreement with previous works indicating that wEST-SSRs are adequate for phylogenetic surveys in the genus. Mapping experiments showed that both, wEST-SSRs and PnSSRs mapped scattered in the genome. On hundred and two new markers were added to the existent P. notatum linkage groups. Primer pairs ksum206, ksum219 and PN03-F2 generated markers that mapped linked to apospory. Sequences of EST-SSRs experimentally mapped in P. notatum showed 46.23% to 55.27% similarity with the original wheat EST. A preliminary comparative mapping analysis was carried combining experimental and in silico mapping results.
Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina
Fil: Sartor, Maria Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina
Materia
Paspalum Spp
Wheat Est-Ssr
Apospory
Comparative Mapping
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6222
Acceder
id CONICETDig_dcb407eebf231fb0d63b84f660dfc476
oai_identifier_str oai:ri.conicet.gov.ar:11336/6222
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in phylogenetic analysis and mappingSiena, Lorena AdelinaSartor, Maria EsperanzaQuarin, Camilo LuisEspinoza, FranciscoOrtiz, Juan Pablo AmelioPaspalum SppWheat Est-SsrAposporyComparative Mappinghttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The genus Paspalum includes numerous species of agronomic importance. The objectives of this work were to evaluate the transferability and polymorphism of publicly available wheat EST-SSR (wEST-SSR) markers to Paspalum spp., assess the cross-species amplification of Paspalum notatum genomic-SSRs (PnSSR) within the genus, and evaluate both types of markers for phylogenetic analyses and mapping. Thirty two accessions, including 11 species were used. Moreover, 65 F1 hybrids of P. notatum were employed for mapping. Transferability ratio of wEST-SSRs was 72.72%. On average 19.25 bands per primer combination were obtained. Cross-species amplification of PnSSRs was 55.37%, with an average of 9.45 fragments per primer pair. Both types of markers differed in the amplification capacity between primers pairs and species. Clustering analysis with wEST-SSRs data discriminated accessions by species and taxonomic groups. Genomic relationships were in agreement with previous works indicating that wEST-SSRs are adequate for phylogenetic surveys in the genus. Mapping experiments showed that both, wEST-SSRs and PnSSRs mapped scattered in the genome. On hundred and two new markers were added to the existent P. notatum linkage groups. Primer pairs ksum206, ksum219 and PN03-F2 generated markers that mapped linked to apospory. Sequences of EST-SSRs experimentally mapped in P. notatum showed 46.23% to 55.27% similarity with the original wheat EST. A preliminary comparative mapping analysis was carried combining experimental and in silico mapping results.Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; ArgentinaFil: Sartor, Maria Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; ArgentinaCrop Science Society of America2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6222Siena, Lorena Adelina; Sartor, Maria Esperanza; Quarin, Camilo Luis; Espinoza, Francisco; Ortiz, Juan Pablo Amelio; Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in phylogenetic analysis and mapping; Crop Science Society of America; Crop Science; 54; 1; 11-2013; 240-2540011-183Xenginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/https://dl.sciencesocieties.org/publications/cs/abstracts/54/1/240info:eu-repo/semantics/altIdentifier/doi/10.2135/cropsci2013.04.0275info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:42:47Zoai:ri.conicet.gov.ar:11336/6222instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:42:47.423CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in phylogenetic analysis and mapping
title Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in phylogenetic analysis and mapping
spellingShingle Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in phylogenetic analysis and mapping
Siena, Lorena Adelina
Paspalum Spp
Wheat Est-Ssr
Apospory
Comparative Mapping
title_short Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in phylogenetic analysis and mapping
title_full Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in phylogenetic analysis and mapping
title_fullStr Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in phylogenetic analysis and mapping
title_full_unstemmed Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in phylogenetic analysis and mapping
title_sort Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in phylogenetic analysis and mapping
dc.creator.none.fl_str_mv Siena, Lorena Adelina
Sartor, Maria Esperanza
Quarin, Camilo Luis
Espinoza, Francisco
Ortiz, Juan Pablo Amelio
author Siena, Lorena Adelina
author_facet Siena, Lorena Adelina
Sartor, Maria Esperanza
Quarin, Camilo Luis
Espinoza, Francisco
Ortiz, Juan Pablo Amelio
author_role author
author2 Sartor, Maria Esperanza
Quarin, Camilo Luis
Espinoza, Francisco
Ortiz, Juan Pablo Amelio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Paspalum Spp
Wheat Est-Ssr
Apospory
Comparative Mapping
topic Paspalum Spp
Wheat Est-Ssr
Apospory
Comparative Mapping
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The genus Paspalum includes numerous species of agronomic importance. The objectives of this work were to evaluate the transferability and polymorphism of publicly available wheat EST-SSR (wEST-SSR) markers to Paspalum spp., assess the cross-species amplification of Paspalum notatum genomic-SSRs (PnSSR) within the genus, and evaluate both types of markers for phylogenetic analyses and mapping. Thirty two accessions, including 11 species were used. Moreover, 65 F1 hybrids of P. notatum were employed for mapping. Transferability ratio of wEST-SSRs was 72.72%. On average 19.25 bands per primer combination were obtained. Cross-species amplification of PnSSRs was 55.37%, with an average of 9.45 fragments per primer pair. Both types of markers differed in the amplification capacity between primers pairs and species. Clustering analysis with wEST-SSRs data discriminated accessions by species and taxonomic groups. Genomic relationships were in agreement with previous works indicating that wEST-SSRs are adequate for phylogenetic surveys in the genus. Mapping experiments showed that both, wEST-SSRs and PnSSRs mapped scattered in the genome. On hundred and two new markers were added to the existent P. notatum linkage groups. Primer pairs ksum206, ksum219 and PN03-F2 generated markers that mapped linked to apospory. Sequences of EST-SSRs experimentally mapped in P. notatum showed 46.23% to 55.27% similarity with the original wheat EST. A preliminary comparative mapping analysis was carried combining experimental and in silico mapping results.
Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina
Fil: Sartor, Maria Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina
description The genus Paspalum includes numerous species of agronomic importance. The objectives of this work were to evaluate the transferability and polymorphism of publicly available wheat EST-SSR (wEST-SSR) markers to Paspalum spp., assess the cross-species amplification of Paspalum notatum genomic-SSRs (PnSSR) within the genus, and evaluate both types of markers for phylogenetic analyses and mapping. Thirty two accessions, including 11 species were used. Moreover, 65 F1 hybrids of P. notatum were employed for mapping. Transferability ratio of wEST-SSRs was 72.72%. On average 19.25 bands per primer combination were obtained. Cross-species amplification of PnSSRs was 55.37%, with an average of 9.45 fragments per primer pair. Both types of markers differed in the amplification capacity between primers pairs and species. Clustering analysis with wEST-SSRs data discriminated accessions by species and taxonomic groups. Genomic relationships were in agreement with previous works indicating that wEST-SSRs are adequate for phylogenetic surveys in the genus. Mapping experiments showed that both, wEST-SSRs and PnSSRs mapped scattered in the genome. On hundred and two new markers were added to the existent P. notatum linkage groups. Primer pairs ksum206, ksum219 and PN03-F2 generated markers that mapped linked to apospory. Sequences of EST-SSRs experimentally mapped in P. notatum showed 46.23% to 55.27% similarity with the original wheat EST. A preliminary comparative mapping analysis was carried combining experimental and in silico mapping results.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6222
Siena, Lorena Adelina; Sartor, Maria Esperanza; Quarin, Camilo Luis; Espinoza, Francisco; Ortiz, Juan Pablo Amelio; Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in phylogenetic analysis and mapping; Crop Science Society of America; Crop Science; 54; 1; 11-2013; 240-254
0011-183X
url http://hdl.handle.net/11336/6222
identifier_str_mv Siena, Lorena Adelina; Sartor, Maria Esperanza; Quarin, Camilo Luis; Espinoza, Francisco; Ortiz, Juan Pablo Amelio; Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in phylogenetic analysis and mapping; Crop Science Society of America; Crop Science; 54; 1; 11-2013; 240-254
0011-183X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/https://dl.sciencesocieties.org/publications/cs/abstracts/54/1/240
info:eu-repo/semantics/altIdentifier/doi/10.2135/cropsci2013.04.0275
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Crop Science Society of America
publisher.none.fl_str_mv Crop Science Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082930433064960
score 13.22299

Publicaciones similares