Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.

Autores
Regalado González, Jose Javier; Carmona Martín, E.; Castro, P.; Moreno, R.; Gil, J.; Encina, C. L.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polyploid plants have been induced in different Asparagus officinalis L. breeding programs in order to obtain plants with improved agronomical traits, such as large spear diameter or segregation ratios with a higher number of males. The polyploidization methods can produce somaclonal variation in the polyploid plants obtained and, therefore, unwanted changes in the agronomical traits of the initial elite plants. We used two different polyploidization methods to induce polyploid plants from diploid genotypes of commercial varieties and tetraploid genotypes of the Spanish landrace “Morado de Huétor”. The first method was the culture of rhizome buds in the medium ARBM-3 (Asparagus Rhizome Bud Medium), supplemented with different concentrations of colchicine (0.1–0.75 g l−1) for 10 and 20 days. The best polyploidization rate obtained was 25 % (0.5 g l−1 colchicine for 10 days). The second method was the regeneration of polyploid plants from callus culture, resulting in a polyploidization rate of 40 and 12.5 % for the diploid genotype CM077 and the tetraploid genotype HT156, respectively. Additionally, we have developed a new protocol to separate the mixoploids generated into their different genetic components, obtaining plants with a unique ploidy level. EST-SSRs markers were employed to analyze the genetic stability of polyploidy plants. Somaclonal variation was not detected for polyploidy plants obtained through the culture of rhizome bud explants. Therefore, these polyploid plants should maintain the agronomical traits of the initial elite plants. However, somaclonal variation was detected in the polyploid plants regenerated from callus culture.
Fil: Regalado González, Jose Javier. Instituto de Horticultura Subtropical y Mediterránea La Mayora; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carmona Martín, E.. Instituto de Horticultura Subtropical y Mediterránea La Mayora; España
Fil: Castro, P.. Universidad de Córdoba; España
Fil: Moreno, R.. Universidad de Córdoba; España
Fil: Gil, J.. Universidad de Córdoba; España
Fil: Encina, C. L.. Instituto de Horticultura Subtropical y Mediterránea La Mayora; España
Materia
Colchicine
Rhizome Buds
Endoreduplication
Est-Ssrs
Organogenesis
Separation of Mixoploids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42869
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/42869
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.Regalado González, Jose JavierCarmona Martín, E.Castro, P.Moreno, R.Gil, J.Encina, C. L.ColchicineRhizome BudsEndoreduplicationEst-SsrsOrganogenesisSeparation of Mixoploidshttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Polyploid plants have been induced in different Asparagus officinalis L. breeding programs in order to obtain plants with improved agronomical traits, such as large spear diameter or segregation ratios with a higher number of males. The polyploidization methods can produce somaclonal variation in the polyploid plants obtained and, therefore, unwanted changes in the agronomical traits of the initial elite plants. We used two different polyploidization methods to induce polyploid plants from diploid genotypes of commercial varieties and tetraploid genotypes of the Spanish landrace “Morado de Huétor”. The first method was the culture of rhizome buds in the medium ARBM-3 (Asparagus Rhizome Bud Medium), supplemented with different concentrations of colchicine (0.1–0.75 g l−1) for 10 and 20 days. The best polyploidization rate obtained was 25 % (0.5 g l−1 colchicine for 10 days). The second method was the regeneration of polyploid plants from callus culture, resulting in a polyploidization rate of 40 and 12.5 % for the diploid genotype CM077 and the tetraploid genotype HT156, respectively. Additionally, we have developed a new protocol to separate the mixoploids generated into their different genetic components, obtaining plants with a unique ploidy level. EST-SSRs markers were employed to analyze the genetic stability of polyploidy plants. Somaclonal variation was not detected for polyploidy plants obtained through the culture of rhizome bud explants. Therefore, these polyploid plants should maintain the agronomical traits of the initial elite plants. However, somaclonal variation was detected in the polyploid plants regenerated from callus culture.Fil: Regalado González, Jose Javier. Instituto de Horticultura Subtropical y Mediterránea La Mayora; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carmona Martín, E.. Instituto de Horticultura Subtropical y Mediterránea La Mayora; EspañaFil: Castro, P.. Universidad de Córdoba; EspañaFil: Moreno, R.. Universidad de Córdoba; EspañaFil: Gil, J.. Universidad de Córdoba; EspañaFil: Encina, C. L.. Instituto de Horticultura Subtropical y Mediterránea La Mayora; EspañaSpringer2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42869Regalado González, Jose Javier; Carmona Martín, E.; Castro, P.; Moreno, R.; Gil, J.; et al.; Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.; Springer; Plant Cell, Tissue and Organ Culture; 122; 1; 6-2015; 31-440167-68571573-5044CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs11240-015-0747-xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-015-0747-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:40:46Zoai:ri.conicet.gov.ar:11336/42869instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:40:46.424CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.
title Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.
spellingShingle Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.
Regalado González, Jose Javier
Colchicine
Rhizome Buds
Endoreduplication
Est-Ssrs
Organogenesis
Separation of Mixoploids
title_short Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.
title_full Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.
title_fullStr Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.
title_full_unstemmed Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.
title_sort Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.
dc.creator.none.fl_str_mv Regalado González, Jose Javier
Carmona Martín, E.
Castro, P.
Moreno, R.
Gil, J.
Encina, C. L.
author Regalado González, Jose Javier
author_facet Regalado González, Jose Javier
Carmona Martín, E.
Castro, P.
Moreno, R.
Gil, J.
Encina, C. L.
author_role author
author2 Carmona Martín, E.
Castro, P.
Moreno, R.
Gil, J.
Encina, C. L.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Colchicine
Rhizome Buds
Endoreduplication
Est-Ssrs
Organogenesis
Separation of Mixoploids
topic Colchicine
Rhizome Buds
Endoreduplication
Est-Ssrs
Organogenesis
Separation of Mixoploids
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Polyploid plants have been induced in different Asparagus officinalis L. breeding programs in order to obtain plants with improved agronomical traits, such as large spear diameter or segregation ratios with a higher number of males. The polyploidization methods can produce somaclonal variation in the polyploid plants obtained and, therefore, unwanted changes in the agronomical traits of the initial elite plants. We used two different polyploidization methods to induce polyploid plants from diploid genotypes of commercial varieties and tetraploid genotypes of the Spanish landrace “Morado de Huétor”. The first method was the culture of rhizome buds in the medium ARBM-3 (Asparagus Rhizome Bud Medium), supplemented with different concentrations of colchicine (0.1–0.75 g l−1) for 10 and 20 days. The best polyploidization rate obtained was 25 % (0.5 g l−1 colchicine for 10 days). The second method was the regeneration of polyploid plants from callus culture, resulting in a polyploidization rate of 40 and 12.5 % for the diploid genotype CM077 and the tetraploid genotype HT156, respectively. Additionally, we have developed a new protocol to separate the mixoploids generated into their different genetic components, obtaining plants with a unique ploidy level. EST-SSRs markers were employed to analyze the genetic stability of polyploidy plants. Somaclonal variation was not detected for polyploidy plants obtained through the culture of rhizome bud explants. Therefore, these polyploid plants should maintain the agronomical traits of the initial elite plants. However, somaclonal variation was detected in the polyploid plants regenerated from callus culture.
Fil: Regalado González, Jose Javier. Instituto de Horticultura Subtropical y Mediterránea La Mayora; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carmona Martín, E.. Instituto de Horticultura Subtropical y Mediterránea La Mayora; España
Fil: Castro, P.. Universidad de Córdoba; España
Fil: Moreno, R.. Universidad de Córdoba; España
Fil: Gil, J.. Universidad de Córdoba; España
Fil: Encina, C. L.. Instituto de Horticultura Subtropical y Mediterránea La Mayora; España
description Polyploid plants have been induced in different Asparagus officinalis L. breeding programs in order to obtain plants with improved agronomical traits, such as large spear diameter or segregation ratios with a higher number of males. The polyploidization methods can produce somaclonal variation in the polyploid plants obtained and, therefore, unwanted changes in the agronomical traits of the initial elite plants. We used two different polyploidization methods to induce polyploid plants from diploid genotypes of commercial varieties and tetraploid genotypes of the Spanish landrace “Morado de Huétor”. The first method was the culture of rhizome buds in the medium ARBM-3 (Asparagus Rhizome Bud Medium), supplemented with different concentrations of colchicine (0.1–0.75 g l−1) for 10 and 20 days. The best polyploidization rate obtained was 25 % (0.5 g l−1 colchicine for 10 days). The second method was the regeneration of polyploid plants from callus culture, resulting in a polyploidization rate of 40 and 12.5 % for the diploid genotype CM077 and the tetraploid genotype HT156, respectively. Additionally, we have developed a new protocol to separate the mixoploids generated into their different genetic components, obtaining plants with a unique ploidy level. EST-SSRs markers were employed to analyze the genetic stability of polyploidy plants. Somaclonal variation was not detected for polyploidy plants obtained through the culture of rhizome bud explants. Therefore, these polyploid plants should maintain the agronomical traits of the initial elite plants. However, somaclonal variation was detected in the polyploid plants regenerated from callus culture.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42869
Regalado González, Jose Javier; Carmona Martín, E.; Castro, P.; Moreno, R.; Gil, J.; et al.; Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.; Springer; Plant Cell, Tissue and Organ Culture; 122; 1; 6-2015; 31-44
0167-6857
1573-5044
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42869
identifier_str_mv Regalado González, Jose Javier; Carmona Martín, E.; Castro, P.; Moreno, R.; Gil, J.; et al.; Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.; Springer; Plant Cell, Tissue and Organ Culture; 122; 1; 6-2015; 31-44
0167-6857
1573-5044
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs11240-015-0747-x
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-015-0747-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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