6 Iodo-d-lactone: A derivative of arachidonic acid with antitumor effects in HT-29 colon cancer cells

Autores
Thomasz, Lisa; Oglio, Andrea Romina; Rossich, Luciano Esteban; Villamar, Sonia; Perona, Marina; Salvarredi, Leonardo Andres; Dagrosa, María Alejandra; Pisarev, Mario Alberto; Juvenal, Guillermo Juan
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: IL-δ (5-hydroxy-6 iodo-8, 11, 14-eicosatrienoic delta lactone) an iodinated arachidonic acid (AA) derivative, is one of the iodolipids biosynthesized by the thyroid. Although IL-δ regulates several thyroid parameters such as cell proliferation and goiter growth it was found that this iodolipid inhibits the growth of other non thyroid cell lines. Objectives: to study the effect of IL-δ on cell proliferation and apoptosis in the colon cancer cell line HT-29. Results: Treatment with IL-δ reduced cell viability in a concentration-dependent manner: 1μM 20%, 5 μM 25%, 10 μM 31 %, 50 μM 47% and caused a significant decrease of PCNA expression (25%). IL-δ had pro-apoptotic effects, evidenced by morphological features of programmed cell death such as: pyknosis, karyorrhexis, cell shrinkage and cell blebbing observed by fluorescence microscopy, an increase in caspase-3 activity and in Bax/Bcl-2 ratio (2.5 after 3 h of treatment). Furthermore, IL-δ increased ROS production (30%) and lipid peroxidation levels (19 %), suggesting that apoptosis could be a result of increased oxidative stress. A maximum increase in c-fos and c-jun protein expression in response to IL-δ was observed 1 h after initiation of the treatment. Treatment with IL-δ blocked HT-29 xenografts growth in nude mice by more than 70% compared with the control group. Conclusion: our study shows that IL-δ inhibits cell growth and induces apoptosis in the colon cancer cell line, HT-29 and opens the possibility that IL-δ could be a potential useful chemotherapy agent.
Fil: Thomasz, Lisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Oglio, Andrea Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Rossich, Luciano Esteban. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Villamar, Sonia. Comisión Nacional de Energía Atómica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Perona, Marina. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dagrosa, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Pisarev, Mario Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Juvenal, Guillermo Juan. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
IODOLIPIDS
IODOLACTONE
ARACHIDONIC ACID
COLON CANCER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/104121
Acceder
id CONICETDig_3f74288e492721a835c9a407009350f8
oai_identifier_str oai:ri.conicet.gov.ar:11336/104121
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling 6 Iodo-d-lactone: A derivative of arachidonic acid with antitumor effects in HT-29 colon cancer cellsThomasz, LisaOglio, Andrea RominaRossich, Luciano EstebanVillamar, SoniaPerona, MarinaSalvarredi, Leonardo AndresDagrosa, María AlejandraPisarev, Mario AlbertoJuvenal, Guillermo JuanIODOLIPIDSIODOLACTONEARACHIDONIC ACIDCOLON CANCERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: IL-δ (5-hydroxy-6 iodo-8, 11, 14-eicosatrienoic delta lactone) an iodinated arachidonic acid (AA) derivative, is one of the iodolipids biosynthesized by the thyroid. Although IL-δ regulates several thyroid parameters such as cell proliferation and goiter growth it was found that this iodolipid inhibits the growth of other non thyroid cell lines. Objectives: to study the effect of IL-δ on cell proliferation and apoptosis in the colon cancer cell line HT-29. Results: Treatment with IL-δ reduced cell viability in a concentration-dependent manner: 1μM 20%, 5 μM 25%, 10 μM 31 %, 50 μM 47% and caused a significant decrease of PCNA expression (25%). IL-δ had pro-apoptotic effects, evidenced by morphological features of programmed cell death such as: pyknosis, karyorrhexis, cell shrinkage and cell blebbing observed by fluorescence microscopy, an increase in caspase-3 activity and in Bax/Bcl-2 ratio (2.5 after 3 h of treatment). Furthermore, IL-δ increased ROS production (30%) and lipid peroxidation levels (19 %), suggesting that apoptosis could be a result of increased oxidative stress. A maximum increase in c-fos and c-jun protein expression in response to IL-δ was observed 1 h after initiation of the treatment. Treatment with IL-δ blocked HT-29 xenografts growth in nude mice by more than 70% compared with the control group. Conclusion: our study shows that IL-δ inhibits cell growth and induces apoptosis in the colon cancer cell line, HT-29 and opens the possibility that IL-δ could be a potential useful chemotherapy agent.Fil: Thomasz, Lisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Oglio, Andrea Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Rossich, Luciano Esteban. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Villamar, Sonia. Comisión Nacional de Energía Atómica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Perona, Marina. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dagrosa, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Pisarev, Mario Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Juvenal, Guillermo Juan. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104121Thomasz, Lisa; Oglio, Andrea Romina; Rossich, Luciano Esteban; Villamar, Sonia; Perona, Marina; et al.; 6 Iodo-d-lactone: A derivative of arachidonic acid with antitumor effects in HT-29 colon cancer cells; Elsevier; Prostaglandins Leukotrienes and Essential Fatty Acids; 88; 4; 4-2013; 273-2800952-3278CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.plefa.com/article/S0952-3278(13)00004-5/fulltextinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.plefa.2013.01.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:09Zoai:ri.conicet.gov.ar:11336/104121instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:09.374CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv 6 Iodo-d-lactone: A derivative of arachidonic acid with antitumor effects in HT-29 colon cancer cells
title 6 Iodo-d-lactone: A derivative of arachidonic acid with antitumor effects in HT-29 colon cancer cells
spellingShingle 6 Iodo-d-lactone: A derivative of arachidonic acid with antitumor effects in HT-29 colon cancer cells
Thomasz, Lisa
IODOLIPIDS
IODOLACTONE
ARACHIDONIC ACID
COLON CANCER
title_short 6 Iodo-d-lactone: A derivative of arachidonic acid with antitumor effects in HT-29 colon cancer cells
title_full 6 Iodo-d-lactone: A derivative of arachidonic acid with antitumor effects in HT-29 colon cancer cells
title_fullStr 6 Iodo-d-lactone: A derivative of arachidonic acid with antitumor effects in HT-29 colon cancer cells
title_full_unstemmed 6 Iodo-d-lactone: A derivative of arachidonic acid with antitumor effects in HT-29 colon cancer cells
title_sort 6 Iodo-d-lactone: A derivative of arachidonic acid with antitumor effects in HT-29 colon cancer cells
dc.creator.none.fl_str_mv Thomasz, Lisa
Oglio, Andrea Romina
Rossich, Luciano Esteban
Villamar, Sonia
Perona, Marina
Salvarredi, Leonardo Andres
Dagrosa, María Alejandra
Pisarev, Mario Alberto
Juvenal, Guillermo Juan
author Thomasz, Lisa
author_facet Thomasz, Lisa
Oglio, Andrea Romina
Rossich, Luciano Esteban
Villamar, Sonia
Perona, Marina
Salvarredi, Leonardo Andres
Dagrosa, María Alejandra
Pisarev, Mario Alberto
Juvenal, Guillermo Juan
author_role author
author2 Oglio, Andrea Romina
Rossich, Luciano Esteban
Villamar, Sonia
Perona, Marina
Salvarredi, Leonardo Andres
Dagrosa, María Alejandra
Pisarev, Mario Alberto
Juvenal, Guillermo Juan
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv IODOLIPIDS
IODOLACTONE
ARACHIDONIC ACID
COLON CANCER
topic IODOLIPIDS
IODOLACTONE
ARACHIDONIC ACID
COLON CANCER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: IL-δ (5-hydroxy-6 iodo-8, 11, 14-eicosatrienoic delta lactone) an iodinated arachidonic acid (AA) derivative, is one of the iodolipids biosynthesized by the thyroid. Although IL-δ regulates several thyroid parameters such as cell proliferation and goiter growth it was found that this iodolipid inhibits the growth of other non thyroid cell lines. Objectives: to study the effect of IL-δ on cell proliferation and apoptosis in the colon cancer cell line HT-29. Results: Treatment with IL-δ reduced cell viability in a concentration-dependent manner: 1μM 20%, 5 μM 25%, 10 μM 31 %, 50 μM 47% and caused a significant decrease of PCNA expression (25%). IL-δ had pro-apoptotic effects, evidenced by morphological features of programmed cell death such as: pyknosis, karyorrhexis, cell shrinkage and cell blebbing observed by fluorescence microscopy, an increase in caspase-3 activity and in Bax/Bcl-2 ratio (2.5 after 3 h of treatment). Furthermore, IL-δ increased ROS production (30%) and lipid peroxidation levels (19 %), suggesting that apoptosis could be a result of increased oxidative stress. A maximum increase in c-fos and c-jun protein expression in response to IL-δ was observed 1 h after initiation of the treatment. Treatment with IL-δ blocked HT-29 xenografts growth in nude mice by more than 70% compared with the control group. Conclusion: our study shows that IL-δ inhibits cell growth and induces apoptosis in the colon cancer cell line, HT-29 and opens the possibility that IL-δ could be a potential useful chemotherapy agent.
Fil: Thomasz, Lisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Oglio, Andrea Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Rossich, Luciano Esteban. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Villamar, Sonia. Comisión Nacional de Energía Atómica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Perona, Marina. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dagrosa, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Pisarev, Mario Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Juvenal, Guillermo Juan. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background: IL-δ (5-hydroxy-6 iodo-8, 11, 14-eicosatrienoic delta lactone) an iodinated arachidonic acid (AA) derivative, is one of the iodolipids biosynthesized by the thyroid. Although IL-δ regulates several thyroid parameters such as cell proliferation and goiter growth it was found that this iodolipid inhibits the growth of other non thyroid cell lines. Objectives: to study the effect of IL-δ on cell proliferation and apoptosis in the colon cancer cell line HT-29. Results: Treatment with IL-δ reduced cell viability in a concentration-dependent manner: 1μM 20%, 5 μM 25%, 10 μM 31 %, 50 μM 47% and caused a significant decrease of PCNA expression (25%). IL-δ had pro-apoptotic effects, evidenced by morphological features of programmed cell death such as: pyknosis, karyorrhexis, cell shrinkage and cell blebbing observed by fluorescence microscopy, an increase in caspase-3 activity and in Bax/Bcl-2 ratio (2.5 after 3 h of treatment). Furthermore, IL-δ increased ROS production (30%) and lipid peroxidation levels (19 %), suggesting that apoptosis could be a result of increased oxidative stress. A maximum increase in c-fos and c-jun protein expression in response to IL-δ was observed 1 h after initiation of the treatment. Treatment with IL-δ blocked HT-29 xenografts growth in nude mice by more than 70% compared with the control group. Conclusion: our study shows that IL-δ inhibits cell growth and induces apoptosis in the colon cancer cell line, HT-29 and opens the possibility that IL-δ could be a potential useful chemotherapy agent.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/104121
Thomasz, Lisa; Oglio, Andrea Romina; Rossich, Luciano Esteban; Villamar, Sonia; Perona, Marina; et al.; 6 Iodo-d-lactone: A derivative of arachidonic acid with antitumor effects in HT-29 colon cancer cells; Elsevier; Prostaglandins Leukotrienes and Essential Fatty Acids; 88; 4; 4-2013; 273-280
0952-3278
CONICET Digital
CONICET
url http://hdl.handle.net/11336/104121
identifier_str_mv Thomasz, Lisa; Oglio, Andrea Romina; Rossich, Luciano Esteban; Villamar, Sonia; Perona, Marina; et al.; 6 Iodo-d-lactone: A derivative of arachidonic acid with antitumor effects in HT-29 colon cancer cells; Elsevier; Prostaglandins Leukotrienes and Essential Fatty Acids; 88; 4; 4-2013; 273-280
0952-3278
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.plefa.com/article/S0952-3278(13)00004-5/fulltext
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plefa.2013.01.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270108519497728
score 13.13397

Publicaciones similares