Inhibitory effects of 2-iodohexadecanal on FRTL-5 thyroid cells proliferation

Autores
Thomasz, Lisa; Coulonval, Katia; Salvarredi, Leonardo Andres; Oglio, Andrea Romina; Fusco, Alfredo; Rossich, Luciano Esteban; Pisarev, Mario Alberto; Roger, Pierre P.; Juvenal, Guillermo Juan
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although thyroid gland function is mainly under the control of pituitary TSH, other factors, such as iodine, play a role in this process. The thyroid is capable of producing different iodolipids such as 6-iodo-deltalactone and 2-iodohexadecanal (2-IHDA). It was shown that these iodolipids mimic some of the inhibitory effects of excess iodide on several thyroid parameters. Objectives: To study the effect of 2-IHDA on cell proliferation and apoptosis in FRTL-5 cells. Results: FRTL-5 cells were grown in the presence of TSH and treated with increasing concentrations of KI and 2-IHDA (0.5, 5, 10 and 33 μM) for 24, 48 and 72 h. Whereas KI inhibited cell proliferation only at 33 μM after 72 h of treatment, 2-IHDA inhibited in a time and concentration dependent manner. Analysis of cell cycle by flow cytometric DNA analysis revealed an accumulation of cells in G1 phase induced by 2-IHDA. The expression of cyclin A, cyclin D1 and cyclin D3 were reduced after treatment with 2-IHDA whereas CDK4 and CDK6 proteins were not modified. 2-IHDA induced a dynamic change in cytoplasmic to nuclear accumulation of p21 and p27 causing these proteins to be accumulated mostly in the nucleus. We also observed evidence of a pro-apoptotic effect of 2-IHDA at highest concentrations. No significant effect of KI was observed. Conclusion: These results suggest that the inhibitory effects of 2-IHDA on FRTL-5 thyroid cell proliferation are mediated by cell cycle arrest in G1/S phase and cell death by apoptosis.
Fil: Thomasz, Lisa. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Coulonval, Katia. Université Libre de Bruxelles; Bélgica
Fil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Oglio, Andrea Romina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fusco, Alfredo. Università degli Studi di Napoli Federico II; Italia
Fil: Rossich, Luciano Esteban. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pisarev, Mario Alberto. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roger, Pierre P.. Université Libre de Bruxelles; Bélgica
Fil: Juvenal, Guillermo Juan. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
IODINE
IODOHEXADECANAL
IODOLIPIDS
THYROID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/180162
Acceder
id CONICETDig_27ec568ee7d1c9468a3104eba052bbf4
oai_identifier_str oai:ri.conicet.gov.ar:11336/180162
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inhibitory effects of 2-iodohexadecanal on FRTL-5 thyroid cells proliferationThomasz, LisaCoulonval, KatiaSalvarredi, Leonardo AndresOglio, Andrea RominaFusco, AlfredoRossich, Luciano EstebanPisarev, Mario AlbertoRoger, Pierre P.Juvenal, Guillermo JuanIODINEIODOHEXADECANALIODOLIPIDSTHYROIDhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Although thyroid gland function is mainly under the control of pituitary TSH, other factors, such as iodine, play a role in this process. The thyroid is capable of producing different iodolipids such as 6-iodo-deltalactone and 2-iodohexadecanal (2-IHDA). It was shown that these iodolipids mimic some of the inhibitory effects of excess iodide on several thyroid parameters. Objectives: To study the effect of 2-IHDA on cell proliferation and apoptosis in FRTL-5 cells. Results: FRTL-5 cells were grown in the presence of TSH and treated with increasing concentrations of KI and 2-IHDA (0.5, 5, 10 and 33 μM) for 24, 48 and 72 h. Whereas KI inhibited cell proliferation only at 33 μM after 72 h of treatment, 2-IHDA inhibited in a time and concentration dependent manner. Analysis of cell cycle by flow cytometric DNA analysis revealed an accumulation of cells in G1 phase induced by 2-IHDA. The expression of cyclin A, cyclin D1 and cyclin D3 were reduced after treatment with 2-IHDA whereas CDK4 and CDK6 proteins were not modified. 2-IHDA induced a dynamic change in cytoplasmic to nuclear accumulation of p21 and p27 causing these proteins to be accumulated mostly in the nucleus. We also observed evidence of a pro-apoptotic effect of 2-IHDA at highest concentrations. No significant effect of KI was observed. Conclusion: These results suggest that the inhibitory effects of 2-IHDA on FRTL-5 thyroid cell proliferation are mediated by cell cycle arrest in G1/S phase and cell death by apoptosis.Fil: Thomasz, Lisa. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Coulonval, Katia. Université Libre de Bruxelles; BélgicaFil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Oglio, Andrea Romina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fusco, Alfredo. Università degli Studi di Napoli Federico II; ItaliaFil: Rossich, Luciano Esteban. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pisarev, Mario Alberto. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Roger, Pierre P.. Université Libre de Bruxelles; BélgicaFil: Juvenal, Guillermo Juan. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Ireland2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180162Thomasz, Lisa; Coulonval, Katia; Salvarredi, Leonardo Andres; Oglio, Andrea Romina; Fusco, Alfredo; et al.; Inhibitory effects of 2-iodohexadecanal on FRTL-5 thyroid cells proliferation; Elsevier Ireland; Molecular and Cellular Endocrinology; 404; 3-2015; 123-1310303-7207CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2015.01.038info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:05Zoai:ri.conicet.gov.ar:11336/180162instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:05.383CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inhibitory effects of 2-iodohexadecanal on FRTL-5 thyroid cells proliferation
title Inhibitory effects of 2-iodohexadecanal on FRTL-5 thyroid cells proliferation
spellingShingle Inhibitory effects of 2-iodohexadecanal on FRTL-5 thyroid cells proliferation
Thomasz, Lisa
IODINE
IODOHEXADECANAL
IODOLIPIDS
THYROID
title_short Inhibitory effects of 2-iodohexadecanal on FRTL-5 thyroid cells proliferation
title_full Inhibitory effects of 2-iodohexadecanal on FRTL-5 thyroid cells proliferation
title_fullStr Inhibitory effects of 2-iodohexadecanal on FRTL-5 thyroid cells proliferation
title_full_unstemmed Inhibitory effects of 2-iodohexadecanal on FRTL-5 thyroid cells proliferation
title_sort Inhibitory effects of 2-iodohexadecanal on FRTL-5 thyroid cells proliferation
dc.creator.none.fl_str_mv Thomasz, Lisa
Coulonval, Katia
Salvarredi, Leonardo Andres
Oglio, Andrea Romina
Fusco, Alfredo
Rossich, Luciano Esteban
Pisarev, Mario Alberto
Roger, Pierre P.
Juvenal, Guillermo Juan
author Thomasz, Lisa
author_facet Thomasz, Lisa
Coulonval, Katia
Salvarredi, Leonardo Andres
Oglio, Andrea Romina
Fusco, Alfredo
Rossich, Luciano Esteban
Pisarev, Mario Alberto
Roger, Pierre P.
Juvenal, Guillermo Juan
author_role author
author2 Coulonval, Katia
Salvarredi, Leonardo Andres
Oglio, Andrea Romina
Fusco, Alfredo
Rossich, Luciano Esteban
Pisarev, Mario Alberto
Roger, Pierre P.
Juvenal, Guillermo Juan
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv IODINE
IODOHEXADECANAL
IODOLIPIDS
THYROID
topic IODINE
IODOHEXADECANAL
IODOLIPIDS
THYROID
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Although thyroid gland function is mainly under the control of pituitary TSH, other factors, such as iodine, play a role in this process. The thyroid is capable of producing different iodolipids such as 6-iodo-deltalactone and 2-iodohexadecanal (2-IHDA). It was shown that these iodolipids mimic some of the inhibitory effects of excess iodide on several thyroid parameters. Objectives: To study the effect of 2-IHDA on cell proliferation and apoptosis in FRTL-5 cells. Results: FRTL-5 cells were grown in the presence of TSH and treated with increasing concentrations of KI and 2-IHDA (0.5, 5, 10 and 33 μM) for 24, 48 and 72 h. Whereas KI inhibited cell proliferation only at 33 μM after 72 h of treatment, 2-IHDA inhibited in a time and concentration dependent manner. Analysis of cell cycle by flow cytometric DNA analysis revealed an accumulation of cells in G1 phase induced by 2-IHDA. The expression of cyclin A, cyclin D1 and cyclin D3 were reduced after treatment with 2-IHDA whereas CDK4 and CDK6 proteins were not modified. 2-IHDA induced a dynamic change in cytoplasmic to nuclear accumulation of p21 and p27 causing these proteins to be accumulated mostly in the nucleus. We also observed evidence of a pro-apoptotic effect of 2-IHDA at highest concentrations. No significant effect of KI was observed. Conclusion: These results suggest that the inhibitory effects of 2-IHDA on FRTL-5 thyroid cell proliferation are mediated by cell cycle arrest in G1/S phase and cell death by apoptosis.
Fil: Thomasz, Lisa. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Coulonval, Katia. Université Libre de Bruxelles; Bélgica
Fil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Oglio, Andrea Romina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fusco, Alfredo. Università degli Studi di Napoli Federico II; Italia
Fil: Rossich, Luciano Esteban. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pisarev, Mario Alberto. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roger, Pierre P.. Université Libre de Bruxelles; Bélgica
Fil: Juvenal, Guillermo Juan. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. División Bioquímica Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Although thyroid gland function is mainly under the control of pituitary TSH, other factors, such as iodine, play a role in this process. The thyroid is capable of producing different iodolipids such as 6-iodo-deltalactone and 2-iodohexadecanal (2-IHDA). It was shown that these iodolipids mimic some of the inhibitory effects of excess iodide on several thyroid parameters. Objectives: To study the effect of 2-IHDA on cell proliferation and apoptosis in FRTL-5 cells. Results: FRTL-5 cells were grown in the presence of TSH and treated with increasing concentrations of KI and 2-IHDA (0.5, 5, 10 and 33 μM) for 24, 48 and 72 h. Whereas KI inhibited cell proliferation only at 33 μM after 72 h of treatment, 2-IHDA inhibited in a time and concentration dependent manner. Analysis of cell cycle by flow cytometric DNA analysis revealed an accumulation of cells in G1 phase induced by 2-IHDA. The expression of cyclin A, cyclin D1 and cyclin D3 were reduced after treatment with 2-IHDA whereas CDK4 and CDK6 proteins were not modified. 2-IHDA induced a dynamic change in cytoplasmic to nuclear accumulation of p21 and p27 causing these proteins to be accumulated mostly in the nucleus. We also observed evidence of a pro-apoptotic effect of 2-IHDA at highest concentrations. No significant effect of KI was observed. Conclusion: These results suggest that the inhibitory effects of 2-IHDA on FRTL-5 thyroid cell proliferation are mediated by cell cycle arrest in G1/S phase and cell death by apoptosis.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/180162
Thomasz, Lisa; Coulonval, Katia; Salvarredi, Leonardo Andres; Oglio, Andrea Romina; Fusco, Alfredo; et al.; Inhibitory effects of 2-iodohexadecanal on FRTL-5 thyroid cells proliferation; Elsevier Ireland; Molecular and Cellular Endocrinology; 404; 3-2015; 123-131
0303-7207
CONICET Digital
CONICET
url http://hdl.handle.net/11336/180162
identifier_str_mv Thomasz, Lisa; Coulonval, Katia; Salvarredi, Leonardo Andres; Oglio, Andrea Romina; Fusco, Alfredo; et al.; Inhibitory effects of 2-iodohexadecanal on FRTL-5 thyroid cells proliferation; Elsevier Ireland; Molecular and Cellular Endocrinology; 404; 3-2015; 123-131
0303-7207
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2015.01.038
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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