6 Iodo-Delta Lactone Inhibits Angiogenesis in Human HT29 Colon Adenocarcinoma xenograft.

Autores
Oglio, Romina; Buschittari, Federico; Salvarredi, Leonardo Andres; Michaux, Jennifer; Rodriguez, Carla; Perona, Marina; Dagrosa, María Alejandra; Juvenal, Guillermo Juan; Thomasz, Lisa
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: Several studies have shown the antiproliferative effect of iodine and 5-hydroxy-6iodo-eicosatrienoic delta lactone (IL-δ) on diverse tissues. It was demonstrated that moleculariodine (I2) and IL-δ, but not iodide (I-), exerts anti-neoplastic actions in different cancers. Theunderlying mechanism through which IL-δ inhibits tumor growth remains unclear. The aim of thisstudy was to analyze the effect of IL-δ on tumor growth and angiogenesis in human HT29colorectal cancer xenografts.Methodology and Results: HT29 cells were injected subcutaneously into the flanks of nude miceand IL-δ was i.p. injected at a dose of 15 μg three days a week. IL-δ treatment in HT29 xenograftsshowed time-dependent inhibition of tumor growth, decrease of mitosis and PCNA expression(p<0.05), increase of P27 expression and Caspase 3 activity after 18 days of treatment (p<0.05). Toassess tumor Microvessel Densities (MVD), CD31 staining by immunohistochemistry wasanalyzed. IL-δ treatment decreased MVD by 17% and 30% after 18 and 30 days respectively(p<0.05), as well as it decreased VEGF and VEGF-R2 expression (p<0.05). Additionally, ourfindings demonstrated that IL-δ increased VEGF-R1 and Ang-1 mRNA levels (p<0.01).Conclusion: The antitumor efficacy of IL-δ in vivo involves inhibition of cell proliferation as wellas induction of apoptosis. IL-δ has also anti-angiogenic effect associated with VEGF and VEGF-R2downregulation followed by Ang-1 and VEGF-R1 increased expression. High levels of Ang-1would contribute to mature vessel stabilization and maintenance while VEGF-R1 increase wouldproduce anti-proliferative effect on endothelial cells.
Fil: Oglio, Romina. Comisión Nacional de Energía Atómica; Argentina
Fil: Buschittari, Federico. Comisión Nacional de Energía Atómica; Argentina
Fil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Michaux, Jennifer. Université Libre de Bruxelles; Bélgica
Fil: Rodriguez, Carla. Comisión Nacional de Energía Atómica; Argentina
Fil: Perona, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Dagrosa, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Juvenal, Guillermo Juan. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Thomasz, Lisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Materia
IODOLIPIDS
IODOLACTONE
COLON CANCER
ANGIOGENESIS.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/220714
Acceder
id CONICETDig_55fa0e6a3f789a390bb176f61dbbfc8b
oai_identifier_str oai:ri.conicet.gov.ar:11336/220714
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling 6 Iodo-Delta Lactone Inhibits Angiogenesis in Human HT29 Colon Adenocarcinoma xenograft.Oglio, RominaBuschittari, FedericoSalvarredi, Leonardo AndresMichaux, JenniferRodriguez, CarlaPerona, MarinaDagrosa, María AlejandraJuvenal, Guillermo JuanThomasz, LisaIODOLIPIDSIODOLACTONECOLON CANCERANGIOGENESIS.https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Introduction: Several studies have shown the antiproliferative effect of iodine and 5-hydroxy-6iodo-eicosatrienoic delta lactone (IL-δ) on diverse tissues. It was demonstrated that moleculariodine (I2) and IL-δ, but not iodide (I-), exerts anti-neoplastic actions in different cancers. Theunderlying mechanism through which IL-δ inhibits tumor growth remains unclear. The aim of thisstudy was to analyze the effect of IL-δ on tumor growth and angiogenesis in human HT29colorectal cancer xenografts.Methodology and Results: HT29 cells were injected subcutaneously into the flanks of nude miceand IL-δ was i.p. injected at a dose of 15 μg three days a week. IL-δ treatment in HT29 xenograftsshowed time-dependent inhibition of tumor growth, decrease of mitosis and PCNA expression(p<0.05), increase of P27 expression and Caspase 3 activity after 18 days of treatment (p<0.05). Toassess tumor Microvessel Densities (MVD), CD31 staining by immunohistochemistry wasanalyzed. IL-δ treatment decreased MVD by 17% and 30% after 18 and 30 days respectively(p<0.05), as well as it decreased VEGF and VEGF-R2 expression (p<0.05). Additionally, ourfindings demonstrated that IL-δ increased VEGF-R1 and Ang-1 mRNA levels (p<0.01).Conclusion: The antitumor efficacy of IL-δ in vivo involves inhibition of cell proliferation as wellas induction of apoptosis. IL-δ has also anti-angiogenic effect associated with VEGF and VEGF-R2downregulation followed by Ang-1 and VEGF-R1 increased expression. High levels of Ang-1would contribute to mature vessel stabilization and maintenance while VEGF-R1 increase wouldproduce anti-proliferative effect on endothelial cells.Fil: Oglio, Romina. Comisión Nacional de Energía Atómica; ArgentinaFil: Buschittari, Federico. Comisión Nacional de Energía Atómica; ArgentinaFil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Michaux, Jennifer. Université Libre de Bruxelles; BélgicaFil: Rodriguez, Carla. Comisión Nacional de Energía Atómica; ArgentinaFil: Perona, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Dagrosa, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Juvenal, Guillermo Juan. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Thomasz, Lisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaElsevier2022-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/220714Oglio, Romina; Buschittari, Federico; Salvarredi, Leonardo Andres; Michaux, Jennifer; Rodriguez, Carla; et al.; 6 Iodo-Delta Lactone Inhibits Angiogenesis in Human HT29 Colon Adenocarcinoma xenograft.; Elsevier; Prostaglandins Leukotrienes and Essential Fatty Acids; 10-2022; 1-250952-3278CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0952327822001193info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plefa.2022.102507info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:20:14Zoai:ri.conicet.gov.ar:11336/220714instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:20:14.66CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv 6 Iodo-Delta Lactone Inhibits Angiogenesis in Human HT29 Colon Adenocarcinoma xenograft.
title 6 Iodo-Delta Lactone Inhibits Angiogenesis in Human HT29 Colon Adenocarcinoma xenograft.
spellingShingle 6 Iodo-Delta Lactone Inhibits Angiogenesis in Human HT29 Colon Adenocarcinoma xenograft.
Oglio, Romina
IODOLIPIDS
IODOLACTONE
COLON CANCER
ANGIOGENESIS.
title_short 6 Iodo-Delta Lactone Inhibits Angiogenesis in Human HT29 Colon Adenocarcinoma xenograft.
title_full 6 Iodo-Delta Lactone Inhibits Angiogenesis in Human HT29 Colon Adenocarcinoma xenograft.
title_fullStr 6 Iodo-Delta Lactone Inhibits Angiogenesis in Human HT29 Colon Adenocarcinoma xenograft.
title_full_unstemmed 6 Iodo-Delta Lactone Inhibits Angiogenesis in Human HT29 Colon Adenocarcinoma xenograft.
title_sort 6 Iodo-Delta Lactone Inhibits Angiogenesis in Human HT29 Colon Adenocarcinoma xenograft.
dc.creator.none.fl_str_mv Oglio, Romina
Buschittari, Federico
Salvarredi, Leonardo Andres
Michaux, Jennifer
Rodriguez, Carla
Perona, Marina
Dagrosa, María Alejandra
Juvenal, Guillermo Juan
Thomasz, Lisa
author Oglio, Romina
author_facet Oglio, Romina
Buschittari, Federico
Salvarredi, Leonardo Andres
Michaux, Jennifer
Rodriguez, Carla
Perona, Marina
Dagrosa, María Alejandra
Juvenal, Guillermo Juan
Thomasz, Lisa
author_role author
author2 Buschittari, Federico
Salvarredi, Leonardo Andres
Michaux, Jennifer
Rodriguez, Carla
Perona, Marina
Dagrosa, María Alejandra
Juvenal, Guillermo Juan
Thomasz, Lisa
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv IODOLIPIDS
IODOLACTONE
COLON CANCER
ANGIOGENESIS.
topic IODOLIPIDS
IODOLACTONE
COLON CANCER
ANGIOGENESIS.
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction: Several studies have shown the antiproliferative effect of iodine and 5-hydroxy-6iodo-eicosatrienoic delta lactone (IL-δ) on diverse tissues. It was demonstrated that moleculariodine (I2) and IL-δ, but not iodide (I-), exerts anti-neoplastic actions in different cancers. Theunderlying mechanism through which IL-δ inhibits tumor growth remains unclear. The aim of thisstudy was to analyze the effect of IL-δ on tumor growth and angiogenesis in human HT29colorectal cancer xenografts.Methodology and Results: HT29 cells were injected subcutaneously into the flanks of nude miceand IL-δ was i.p. injected at a dose of 15 μg three days a week. IL-δ treatment in HT29 xenograftsshowed time-dependent inhibition of tumor growth, decrease of mitosis and PCNA expression(p<0.05), increase of P27 expression and Caspase 3 activity after 18 days of treatment (p<0.05). Toassess tumor Microvessel Densities (MVD), CD31 staining by immunohistochemistry wasanalyzed. IL-δ treatment decreased MVD by 17% and 30% after 18 and 30 days respectively(p<0.05), as well as it decreased VEGF and VEGF-R2 expression (p<0.05). Additionally, ourfindings demonstrated that IL-δ increased VEGF-R1 and Ang-1 mRNA levels (p<0.01).Conclusion: The antitumor efficacy of IL-δ in vivo involves inhibition of cell proliferation as wellas induction of apoptosis. IL-δ has also anti-angiogenic effect associated with VEGF and VEGF-R2downregulation followed by Ang-1 and VEGF-R1 increased expression. High levels of Ang-1would contribute to mature vessel stabilization and maintenance while VEGF-R1 increase wouldproduce anti-proliferative effect on endothelial cells.
Fil: Oglio, Romina. Comisión Nacional de Energía Atómica; Argentina
Fil: Buschittari, Federico. Comisión Nacional de Energía Atómica; Argentina
Fil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Michaux, Jennifer. Université Libre de Bruxelles; Bélgica
Fil: Rodriguez, Carla. Comisión Nacional de Energía Atómica; Argentina
Fil: Perona, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Dagrosa, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Juvenal, Guillermo Juan. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Thomasz, Lisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
description Introduction: Several studies have shown the antiproliferative effect of iodine and 5-hydroxy-6iodo-eicosatrienoic delta lactone (IL-δ) on diverse tissues. It was demonstrated that moleculariodine (I2) and IL-δ, but not iodide (I-), exerts anti-neoplastic actions in different cancers. Theunderlying mechanism through which IL-δ inhibits tumor growth remains unclear. The aim of thisstudy was to analyze the effect of IL-δ on tumor growth and angiogenesis in human HT29colorectal cancer xenografts.Methodology and Results: HT29 cells were injected subcutaneously into the flanks of nude miceand IL-δ was i.p. injected at a dose of 15 μg three days a week. IL-δ treatment in HT29 xenograftsshowed time-dependent inhibition of tumor growth, decrease of mitosis and PCNA expression(p<0.05), increase of P27 expression and Caspase 3 activity after 18 days of treatment (p<0.05). Toassess tumor Microvessel Densities (MVD), CD31 staining by immunohistochemistry wasanalyzed. IL-δ treatment decreased MVD by 17% and 30% after 18 and 30 days respectively(p<0.05), as well as it decreased VEGF and VEGF-R2 expression (p<0.05). Additionally, ourfindings demonstrated that IL-δ increased VEGF-R1 and Ang-1 mRNA levels (p<0.01).Conclusion: The antitumor efficacy of IL-δ in vivo involves inhibition of cell proliferation as wellas induction of apoptosis. IL-δ has also anti-angiogenic effect associated with VEGF and VEGF-R2downregulation followed by Ang-1 and VEGF-R1 increased expression. High levels of Ang-1would contribute to mature vessel stabilization and maintenance while VEGF-R1 increase wouldproduce anti-proliferative effect on endothelial cells.
publishDate 2022
dc.date.none.fl_str_mv 2022-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/220714
Oglio, Romina; Buschittari, Federico; Salvarredi, Leonardo Andres; Michaux, Jennifer; Rodriguez, Carla; et al.; 6 Iodo-Delta Lactone Inhibits Angiogenesis in Human HT29 Colon Adenocarcinoma xenograft.; Elsevier; Prostaglandins Leukotrienes and Essential Fatty Acids; 10-2022; 1-25
0952-3278
CONICET Digital
CONICET
url http://hdl.handle.net/11336/220714
identifier_str_mv Oglio, Romina; Buschittari, Federico; Salvarredi, Leonardo Andres; Michaux, Jennifer; Rodriguez, Carla; et al.; 6 Iodo-Delta Lactone Inhibits Angiogenesis in Human HT29 Colon Adenocarcinoma xenograft.; Elsevier; Prostaglandins Leukotrienes and Essential Fatty Acids; 10-2022; 1-25
0952-3278
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0952327822001193
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plefa.2022.102507
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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