Purification and characterization of patagonfibrase, a metalloproteinase showing α-fibrinogenolytic and hemorrhagic activities, from Philodryas patagoniensis snake venom

Autores
Peichoto, María Elisa; Teibler, Pamela; Mackessy, S.; Leiva, Laura Cristina Ana; Acosta, Ofelia Cristina; Gonçalves, L. R. C.; Tanaka Azevedo, A.; Santoro, M.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Venoms of Colubridae snakes are a rich source of novel compounds, which may have applications in medicine and biochemistry. In the present study, we describe the purification and characterization of a metalloproteinase (patagonfibrase), the first protein to be isolated fromPhilodryas patagoniensis (Colubridae) snake venom. Patagonfibrase is a single-chain protein, showing a molecular mass of 53,224 Da and an acidic isoelectric point (5.8). It hydrolyzed selectively the Aá-chain of fibrinogen and when incubated with fibrinogen or plasma, the thrombin clotting time was prolonged. Prominent hemorrhage developed in mouse skin after intradermal injection of patagonfibrase. When administered into mouse gastrocnemius muscle, it induced local hemorrhage and necrosis, and systemic bleeding in lungs. Patagonfibrase showed proteolytic activity toward azocasein, which was enhanced by Ca 2+ and inhibited by Zn2+, cysteine, dithiothreitol and Na2EDTA. Patagonfibrase impaired platelet aggregation induced by collagen and ADP. Thus, patagonfibrase may play a key role in the pathogenesis of disturbances that occur in P. patagoniensis envenomation, and may be used as a biological tool to explore many facets of hemostasis.
Fil: Peichoto, María Elisa. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Teibler, Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Mackessy, S.. Univeristy Of Northem Colorado. School Of Biological Sciences; Estados Unidos
Fil: Leiva, Laura Cristina Ana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina
Fil: Acosta, Ofelia Cristina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Gonçalves, L. R. C.. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan. Laboratorio de Fisiopatologia; Brasil
Fil: Tanaka Azevedo, A.. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan. Laboratorio de Fisiopatologia; Brasil
Fil: Santoro, M.. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan. Laboratorio de Fisiopatologia; Brasil
Materia
COLUBRID SNAKE VENOM
FIBRINOGEN
HEMORRHAGE
METALLOPROTEINASE
COAGULATION
PLATELETS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242028
Acceder
id CONICETDig_3e5a715399bec8b6752db634d64a64b3
oai_identifier_str oai:ri.conicet.gov.ar:11336/242028
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Purification and characterization of patagonfibrase, a metalloproteinase showing α-fibrinogenolytic and hemorrhagic activities, from Philodryas patagoniensis snake venomPeichoto, María ElisaTeibler, PamelaMackessy, S.Leiva, Laura Cristina AnaAcosta, Ofelia CristinaGonçalves, L. R. C.Tanaka Azevedo, A.Santoro, M.COLUBRID SNAKE VENOMFIBRINOGENHEMORRHAGEMETALLOPROTEINASECOAGULATIONPLATELETShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Venoms of Colubridae snakes are a rich source of novel compounds, which may have applications in medicine and biochemistry. In the present study, we describe the purification and characterization of a metalloproteinase (patagonfibrase), the first protein to be isolated fromPhilodryas patagoniensis (Colubridae) snake venom. Patagonfibrase is a single-chain protein, showing a molecular mass of 53,224 Da and an acidic isoelectric point (5.8). It hydrolyzed selectively the Aá-chain of fibrinogen and when incubated with fibrinogen or plasma, the thrombin clotting time was prolonged. Prominent hemorrhage developed in mouse skin after intradermal injection of patagonfibrase. When administered into mouse gastrocnemius muscle, it induced local hemorrhage and necrosis, and systemic bleeding in lungs. Patagonfibrase showed proteolytic activity toward azocasein, which was enhanced by Ca 2+ and inhibited by Zn2+, cysteine, dithiothreitol and Na2EDTA. Patagonfibrase impaired platelet aggregation induced by collagen and ADP. Thus, patagonfibrase may play a key role in the pathogenesis of disturbances that occur in P. patagoniensis envenomation, and may be used as a biological tool to explore many facets of hemostasis.Fil: Peichoto, María Elisa. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Teibler, Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Mackessy, S.. Univeristy Of Northem Colorado. School Of Biological Sciences; Estados UnidosFil: Leiva, Laura Cristina Ana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; ArgentinaFil: Acosta, Ofelia Cristina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Gonçalves, L. R. C.. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan. Laboratorio de Fisiopatologia; BrasilFil: Tanaka Azevedo, A.. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan. Laboratorio de Fisiopatologia; BrasilFil: Santoro, M.. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan. Laboratorio de Fisiopatologia; BrasilElsevier Science2007-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242028Peichoto, María Elisa; Teibler, Pamela; Mackessy, S.; Leiva, Laura Cristina Ana; Acosta, Ofelia Cristina; et al.; Purification and characterization of patagonfibrase, a metalloproteinase showing α-fibrinogenolytic and hemorrhagic activities, from Philodryas patagoniensis snake venom; Elsevier Science; Biochimica et Biophysica Acta - General Subjects; 1770; 5; 1-2007; 810-8190304-4165CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304416506003850info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbagen.2006.12.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:38:58Zoai:ri.conicet.gov.ar:11336/242028instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:38:58.452CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Purification and characterization of patagonfibrase, a metalloproteinase showing α-fibrinogenolytic and hemorrhagic activities, from Philodryas patagoniensis snake venom
title Purification and characterization of patagonfibrase, a metalloproteinase showing α-fibrinogenolytic and hemorrhagic activities, from Philodryas patagoniensis snake venom
spellingShingle Purification and characterization of patagonfibrase, a metalloproteinase showing α-fibrinogenolytic and hemorrhagic activities, from Philodryas patagoniensis snake venom
Peichoto, María Elisa
COLUBRID SNAKE VENOM
FIBRINOGEN
HEMORRHAGE
METALLOPROTEINASE
COAGULATION
PLATELETS
title_short Purification and characterization of patagonfibrase, a metalloproteinase showing α-fibrinogenolytic and hemorrhagic activities, from Philodryas patagoniensis snake venom
title_full Purification and characterization of patagonfibrase, a metalloproteinase showing α-fibrinogenolytic and hemorrhagic activities, from Philodryas patagoniensis snake venom
title_fullStr Purification and characterization of patagonfibrase, a metalloproteinase showing α-fibrinogenolytic and hemorrhagic activities, from Philodryas patagoniensis snake venom
title_full_unstemmed Purification and characterization of patagonfibrase, a metalloproteinase showing α-fibrinogenolytic and hemorrhagic activities, from Philodryas patagoniensis snake venom
title_sort Purification and characterization of patagonfibrase, a metalloproteinase showing α-fibrinogenolytic and hemorrhagic activities, from Philodryas patagoniensis snake venom
dc.creator.none.fl_str_mv Peichoto, María Elisa
Teibler, Pamela
Mackessy, S.
Leiva, Laura Cristina Ana
Acosta, Ofelia Cristina
Gonçalves, L. R. C.
Tanaka Azevedo, A.
Santoro, M.
author Peichoto, María Elisa
author_facet Peichoto, María Elisa
Teibler, Pamela
Mackessy, S.
Leiva, Laura Cristina Ana
Acosta, Ofelia Cristina
Gonçalves, L. R. C.
Tanaka Azevedo, A.
Santoro, M.
author_role author
author2 Teibler, Pamela
Mackessy, S.
Leiva, Laura Cristina Ana
Acosta, Ofelia Cristina
Gonçalves, L. R. C.
Tanaka Azevedo, A.
Santoro, M.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COLUBRID SNAKE VENOM
FIBRINOGEN
HEMORRHAGE
METALLOPROTEINASE
COAGULATION
PLATELETS
topic COLUBRID SNAKE VENOM
FIBRINOGEN
HEMORRHAGE
METALLOPROTEINASE
COAGULATION
PLATELETS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Venoms of Colubridae snakes are a rich source of novel compounds, which may have applications in medicine and biochemistry. In the present study, we describe the purification and characterization of a metalloproteinase (patagonfibrase), the first protein to be isolated fromPhilodryas patagoniensis (Colubridae) snake venom. Patagonfibrase is a single-chain protein, showing a molecular mass of 53,224 Da and an acidic isoelectric point (5.8). It hydrolyzed selectively the Aá-chain of fibrinogen and when incubated with fibrinogen or plasma, the thrombin clotting time was prolonged. Prominent hemorrhage developed in mouse skin after intradermal injection of patagonfibrase. When administered into mouse gastrocnemius muscle, it induced local hemorrhage and necrosis, and systemic bleeding in lungs. Patagonfibrase showed proteolytic activity toward azocasein, which was enhanced by Ca 2+ and inhibited by Zn2+, cysteine, dithiothreitol and Na2EDTA. Patagonfibrase impaired platelet aggregation induced by collagen and ADP. Thus, patagonfibrase may play a key role in the pathogenesis of disturbances that occur in P. patagoniensis envenomation, and may be used as a biological tool to explore many facets of hemostasis.
Fil: Peichoto, María Elisa. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Teibler, Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Mackessy, S.. Univeristy Of Northem Colorado. School Of Biological Sciences; Estados Unidos
Fil: Leiva, Laura Cristina Ana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina
Fil: Acosta, Ofelia Cristina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Gonçalves, L. R. C.. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan. Laboratorio de Fisiopatologia; Brasil
Fil: Tanaka Azevedo, A.. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan. Laboratorio de Fisiopatologia; Brasil
Fil: Santoro, M.. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan. Laboratorio de Fisiopatologia; Brasil
description Venoms of Colubridae snakes are a rich source of novel compounds, which may have applications in medicine and biochemistry. In the present study, we describe the purification and characterization of a metalloproteinase (patagonfibrase), the first protein to be isolated fromPhilodryas patagoniensis (Colubridae) snake venom. Patagonfibrase is a single-chain protein, showing a molecular mass of 53,224 Da and an acidic isoelectric point (5.8). It hydrolyzed selectively the Aá-chain of fibrinogen and when incubated with fibrinogen or plasma, the thrombin clotting time was prolonged. Prominent hemorrhage developed in mouse skin after intradermal injection of patagonfibrase. When administered into mouse gastrocnemius muscle, it induced local hemorrhage and necrosis, and systemic bleeding in lungs. Patagonfibrase showed proteolytic activity toward azocasein, which was enhanced by Ca 2+ and inhibited by Zn2+, cysteine, dithiothreitol and Na2EDTA. Patagonfibrase impaired platelet aggregation induced by collagen and ADP. Thus, patagonfibrase may play a key role in the pathogenesis of disturbances that occur in P. patagoniensis envenomation, and may be used as a biological tool to explore many facets of hemostasis.
publishDate 2007
dc.date.none.fl_str_mv 2007-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242028
Peichoto, María Elisa; Teibler, Pamela; Mackessy, S.; Leiva, Laura Cristina Ana; Acosta, Ofelia Cristina; et al.; Purification and characterization of patagonfibrase, a metalloproteinase showing α-fibrinogenolytic and hemorrhagic activities, from Philodryas patagoniensis snake venom; Elsevier Science; Biochimica et Biophysica Acta - General Subjects; 1770; 5; 1-2007; 810-819
0304-4165
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242028
identifier_str_mv Peichoto, María Elisa; Teibler, Pamela; Mackessy, S.; Leiva, Laura Cristina Ana; Acosta, Ofelia Cristina; et al.; Purification and characterization of patagonfibrase, a metalloproteinase showing α-fibrinogenolytic and hemorrhagic activities, from Philodryas patagoniensis snake venom; Elsevier Science; Biochimica et Biophysica Acta - General Subjects; 1770; 5; 1-2007; 810-819
0304-4165
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304416506003850
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbagen.2006.12.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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