Purification and characterization of a cysteine-rich secretory protein from Philodryas patagoniensis snake venom

Autores
Peichoto, María Elisa; Mackessy, Stephen P.; Teibler, Gladys Pamela; Tavares, Flávio L.; Burckhardt, Paula L.; Breno, María C.; Acosta, Ofelia Cristina; Santoro, Marcelo Larami
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cysteine-rich secretory proteins (CRiSPs) are widespread in reptile venoms, but most have functions that remain unknown. In the present study we describe the purification and characterization of a CRiSP (patagonin) from the venom of the rear-fanged snake Philodryas patagoniensis, and demonstrate its biological activity. Patagonin is a single-chain protein, exhibiting a molecular mass of 24,858.6 Da, whose NH2-terminal and MS/MS-derived sequences are nearly identical to other snake venom CRiSPs. The purified protein hydrolyzed neither azocasein nor fibrinogen, and it could induce no edema, hemorrhage or inhibition of platelet adhesion and aggregation. In addition, patagonin did not inhibit contractions of rat aortic smooth muscle induced by high K+. However, it caused muscular damage to murine gastrocnemius muscle, an action that has not been previously described for any snake venom CRiSPs. Thus, patagonin will be important for studies of the structure-function and evolutionary relationships of this family of proteins that are widely distributed among snake venoms. © 2009 Elsevier Inc. All rights reserved.
Fil: Peichoto, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste; Argentina. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Mackessy, Stephen P.. Univeristy of Northern Colorado; Estados Unidos
Fil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste; Argentina
Fil: Tavares, Flávio L.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Burckhardt, Paula L.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Breno, María C.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Acosta, Ofelia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste; Argentina
Fil: Santoro, Marcelo Larami. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Materia
Colubridae
Crisp
Myotoxicity
Rear-Fanged Snake Venom
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61317
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/61317
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Purification and characterization of a cysteine-rich secretory protein from Philodryas patagoniensis snake venomPeichoto, María ElisaMackessy, Stephen P.Teibler, Gladys PamelaTavares, Flávio L.Burckhardt, Paula L.Breno, María C.Acosta, Ofelia CristinaSantoro, Marcelo LaramiColubridaeCrispMyotoxicityRear-Fanged Snake Venomhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Cysteine-rich secretory proteins (CRiSPs) are widespread in reptile venoms, but most have functions that remain unknown. In the present study we describe the purification and characterization of a CRiSP (patagonin) from the venom of the rear-fanged snake Philodryas patagoniensis, and demonstrate its biological activity. Patagonin is a single-chain protein, exhibiting a molecular mass of 24,858.6 Da, whose NH2-terminal and MS/MS-derived sequences are nearly identical to other snake venom CRiSPs. The purified protein hydrolyzed neither azocasein nor fibrinogen, and it could induce no edema, hemorrhage or inhibition of platelet adhesion and aggregation. In addition, patagonin did not inhibit contractions of rat aortic smooth muscle induced by high K+. However, it caused muscular damage to murine gastrocnemius muscle, an action that has not been previously described for any snake venom CRiSPs. Thus, patagonin will be important for studies of the structure-function and evolutionary relationships of this family of proteins that are widely distributed among snake venoms. © 2009 Elsevier Inc. All rights reserved.Fil: Peichoto, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste; Argentina. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Mackessy, Stephen P.. Univeristy of Northern Colorado; Estados UnidosFil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste; ArgentinaFil: Tavares, Flávio L.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Burckhardt, Paula L.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Breno, María C.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Acosta, Ofelia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste; ArgentinaFil: Santoro, Marcelo Larami. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilElsevier Science Inc2009-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61317Peichoto, María Elisa; Mackessy, Stephen P.; Teibler, Gladys Pamela; Tavares, Flávio L.; Burckhardt, Paula L.; et al.; Purification and characterization of a cysteine-rich secretory protein from Philodryas patagoniensis snake venom; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 150; 1; 7-2009; 79-841532-0456CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpc.2009.03.002info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045609000581info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:08Zoai:ri.conicet.gov.ar:11336/61317instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:08.986CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Purification and characterization of a cysteine-rich secretory protein from Philodryas patagoniensis snake venom
title Purification and characterization of a cysteine-rich secretory protein from Philodryas patagoniensis snake venom
spellingShingle Purification and characterization of a cysteine-rich secretory protein from Philodryas patagoniensis snake venom
Peichoto, María Elisa
Colubridae
Crisp
Myotoxicity
Rear-Fanged Snake Venom
title_short Purification and characterization of a cysteine-rich secretory protein from Philodryas patagoniensis snake venom
title_full Purification and characterization of a cysteine-rich secretory protein from Philodryas patagoniensis snake venom
title_fullStr Purification and characterization of a cysteine-rich secretory protein from Philodryas patagoniensis snake venom
title_full_unstemmed Purification and characterization of a cysteine-rich secretory protein from Philodryas patagoniensis snake venom
title_sort Purification and characterization of a cysteine-rich secretory protein from Philodryas patagoniensis snake venom
dc.creator.none.fl_str_mv Peichoto, María Elisa
Mackessy, Stephen P.
Teibler, Gladys Pamela
Tavares, Flávio L.
Burckhardt, Paula L.
Breno, María C.
Acosta, Ofelia Cristina
Santoro, Marcelo Larami
author Peichoto, María Elisa
author_facet Peichoto, María Elisa
Mackessy, Stephen P.
Teibler, Gladys Pamela
Tavares, Flávio L.
Burckhardt, Paula L.
Breno, María C.
Acosta, Ofelia Cristina
Santoro, Marcelo Larami
author_role author
author2 Mackessy, Stephen P.
Teibler, Gladys Pamela
Tavares, Flávio L.
Burckhardt, Paula L.
Breno, María C.
Acosta, Ofelia Cristina
Santoro, Marcelo Larami
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Colubridae
Crisp
Myotoxicity
Rear-Fanged Snake Venom
topic Colubridae
Crisp
Myotoxicity
Rear-Fanged Snake Venom
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Cysteine-rich secretory proteins (CRiSPs) are widespread in reptile venoms, but most have functions that remain unknown. In the present study we describe the purification and characterization of a CRiSP (patagonin) from the venom of the rear-fanged snake Philodryas patagoniensis, and demonstrate its biological activity. Patagonin is a single-chain protein, exhibiting a molecular mass of 24,858.6 Da, whose NH2-terminal and MS/MS-derived sequences are nearly identical to other snake venom CRiSPs. The purified protein hydrolyzed neither azocasein nor fibrinogen, and it could induce no edema, hemorrhage or inhibition of platelet adhesion and aggregation. In addition, patagonin did not inhibit contractions of rat aortic smooth muscle induced by high K+. However, it caused muscular damage to murine gastrocnemius muscle, an action that has not been previously described for any snake venom CRiSPs. Thus, patagonin will be important for studies of the structure-function and evolutionary relationships of this family of proteins that are widely distributed among snake venoms. © 2009 Elsevier Inc. All rights reserved.
Fil: Peichoto, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste; Argentina. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Mackessy, Stephen P.. Univeristy of Northern Colorado; Estados Unidos
Fil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste; Argentina
Fil: Tavares, Flávio L.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Burckhardt, Paula L.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Breno, María C.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Acosta, Ofelia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste; Argentina
Fil: Santoro, Marcelo Larami. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
description Cysteine-rich secretory proteins (CRiSPs) are widespread in reptile venoms, but most have functions that remain unknown. In the present study we describe the purification and characterization of a CRiSP (patagonin) from the venom of the rear-fanged snake Philodryas patagoniensis, and demonstrate its biological activity. Patagonin is a single-chain protein, exhibiting a molecular mass of 24,858.6 Da, whose NH2-terminal and MS/MS-derived sequences are nearly identical to other snake venom CRiSPs. The purified protein hydrolyzed neither azocasein nor fibrinogen, and it could induce no edema, hemorrhage or inhibition of platelet adhesion and aggregation. In addition, patagonin did not inhibit contractions of rat aortic smooth muscle induced by high K+. However, it caused muscular damage to murine gastrocnemius muscle, an action that has not been previously described for any snake venom CRiSPs. Thus, patagonin will be important for studies of the structure-function and evolutionary relationships of this family of proteins that are widely distributed among snake venoms. © 2009 Elsevier Inc. All rights reserved.
publishDate 2009
dc.date.none.fl_str_mv 2009-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61317
Peichoto, María Elisa; Mackessy, Stephen P.; Teibler, Gladys Pamela; Tavares, Flávio L.; Burckhardt, Paula L.; et al.; Purification and characterization of a cysteine-rich secretory protein from Philodryas patagoniensis snake venom; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 150; 1; 7-2009; 79-84
1532-0456
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61317
identifier_str_mv Peichoto, María Elisa; Mackessy, Stephen P.; Teibler, Gladys Pamela; Tavares, Flávio L.; Burckhardt, Paula L.; et al.; Purification and characterization of a cysteine-rich secretory protein from Philodryas patagoniensis snake venom; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 150; 1; 7-2009; 79-84
1532-0456
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpc.2009.03.002
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045609000581
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613711382183936
score 13.070432

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