Biochemical and biological analysis of Philodryas baroni (Baron´s Green Racer; Dipsadidae) venom: relevance of the findings to human risk assessment

Autores
Sanchez, Matias Nicolas; Timoniuk, A.; Maruñak, S.; Teibler, P.; Acosta, Ofelia Cristina; Peichoto, María Elisa
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Philodryas baroni - an attractively colored snake - has become readily available through the exotic pet trade. Most people consider this species harmless; however, it has already caused human envenomation. As little is known about the venom from this South-American opisthoglyphous "colubrid" snake, herein, we studied its protein composition by SDS-PAGE, as well as its effects on the hemostatic system. Both reducing and nonreducing SDS-PAGE analysis demonstrated that the venom exhibits greatest complexity in the range of 50-80 kDa. The venom displayed proteolytic activity towards azocollagen, with a specific activity of 75.5 U/ mg, and rapidly hydrolyzed the Aa-chain of fibrinogen, exhibiting lower activity towards the Bb- and g-chains. Philodryas baroni venom showed no platelet pro-aggregating activity per se, but it inhibited collagen- and thrombin-induced platelet aggregation. Prominent hemorrhage developed in mouse skin after intradermal injection of the crude venom, and its minimum hemorrhagic dose was 13.9 mg. When injected intramuscularly into the gastrocnemius of mice, the venom induced local effects such as hemorrhage, myonecrosis, edema and leucocyte infiltration. Due to its venom toxicity shown herein, P. baroni should be considered dangerous to humans and any medically significant bite should be promptly reviewed by a qualified health professional.
Fil: Sanchez, Matias Nicolas. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; Argentina
Fil: Timoniuk, A.. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina
Fil: Maruñak, S.. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina
Fil: Teibler, P.. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina
Fil: Acosta, Ofelia Cristina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; Argentina
Fil: Peichoto, María Elisa. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; Argentina
Materia
Colubridae
Rear-Fanged Snake
Hemorrhage
Coagulation
Platelets
Dipsadidae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8334
Acceder
id CONICETDig_17e732109f7fb57a43ae6fe59c390189
oai_identifier_str oai:ri.conicet.gov.ar:11336/8334
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biochemical and biological analysis of Philodryas baroni (Baron´s Green Racer; Dipsadidae) venom: relevance of the findings to human risk assessmentSanchez, Matias NicolasTimoniuk, A.Maruñak, S.Teibler, P.Acosta, Ofelia CristinaPeichoto, María ElisaColubridaeRear-Fanged SnakeHemorrhageCoagulationPlateletsDipsadidaehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Philodryas baroni - an attractively colored snake - has become readily available through the exotic pet trade. Most people consider this species harmless; however, it has already caused human envenomation. As little is known about the venom from this South-American opisthoglyphous "colubrid" snake, herein, we studied its protein composition by SDS-PAGE, as well as its effects on the hemostatic system. Both reducing and nonreducing SDS-PAGE analysis demonstrated that the venom exhibits greatest complexity in the range of 50-80 kDa. The venom displayed proteolytic activity towards azocollagen, with a specific activity of 75.5 U/ mg, and rapidly hydrolyzed the Aa-chain of fibrinogen, exhibiting lower activity towards the Bb- and g-chains. Philodryas baroni venom showed no platelet pro-aggregating activity per se, but it inhibited collagen- and thrombin-induced platelet aggregation. Prominent hemorrhage developed in mouse skin after intradermal injection of the crude venom, and its minimum hemorrhagic dose was 13.9 mg. When injected intramuscularly into the gastrocnemius of mice, the venom induced local effects such as hemorrhage, myonecrosis, edema and leucocyte infiltration. Due to its venom toxicity shown herein, P. baroni should be considered dangerous to humans and any medically significant bite should be promptly reviewed by a qualified health professional.Fil: Sanchez, Matias Nicolas. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; ArgentinaFil: Timoniuk, A.. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; ArgentinaFil: Maruñak, S.. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; ArgentinaFil: Teibler, P.. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; ArgentinaFil: Acosta, Ofelia Cristina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; ArgentinaFil: Peichoto, María Elisa. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; ArgentinaSage Publications Ltd2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8334Sanchez, Matias Nicolas; Timoniuk, A.; Maruñak, S.; Teibler, P.; Acosta, Ofelia Cristina; et al.; Biochemical and biological analysis of Philodryas baroni (Baron´s Green Racer; Dipsadidae) venom: relevance of the findings to human risk assessment; Sage Publications Ltd; Human And Experimental Toxicoloxy; 33; 1; 6-2013; 22-311477-09030960-3271enginfo:eu-repo/semantics/altIdentifier/url/http://het.sagepub.com/content/33/1/22info:eu-repo/semantics/altIdentifier/doi/10.1177/0960327113493302info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:09:43Zoai:ri.conicet.gov.ar:11336/8334instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:09:43.427CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biochemical and biological analysis of Philodryas baroni (Baron´s Green Racer; Dipsadidae) venom: relevance of the findings to human risk assessment
title Biochemical and biological analysis of Philodryas baroni (Baron´s Green Racer; Dipsadidae) venom: relevance of the findings to human risk assessment
spellingShingle Biochemical and biological analysis of Philodryas baroni (Baron´s Green Racer; Dipsadidae) venom: relevance of the findings to human risk assessment
Sanchez, Matias Nicolas
Colubridae
Rear-Fanged Snake
Hemorrhage
Coagulation
Platelets
Dipsadidae
title_short Biochemical and biological analysis of Philodryas baroni (Baron´s Green Racer; Dipsadidae) venom: relevance of the findings to human risk assessment
title_full Biochemical and biological analysis of Philodryas baroni (Baron´s Green Racer; Dipsadidae) venom: relevance of the findings to human risk assessment
title_fullStr Biochemical and biological analysis of Philodryas baroni (Baron´s Green Racer; Dipsadidae) venom: relevance of the findings to human risk assessment
title_full_unstemmed Biochemical and biological analysis of Philodryas baroni (Baron´s Green Racer; Dipsadidae) venom: relevance of the findings to human risk assessment
title_sort Biochemical and biological analysis of Philodryas baroni (Baron´s Green Racer; Dipsadidae) venom: relevance of the findings to human risk assessment
dc.creator.none.fl_str_mv Sanchez, Matias Nicolas
Timoniuk, A.
Maruñak, S.
Teibler, P.
Acosta, Ofelia Cristina
Peichoto, María Elisa
author Sanchez, Matias Nicolas
author_facet Sanchez, Matias Nicolas
Timoniuk, A.
Maruñak, S.
Teibler, P.
Acosta, Ofelia Cristina
Peichoto, María Elisa
author_role author
author2 Timoniuk, A.
Maruñak, S.
Teibler, P.
Acosta, Ofelia Cristina
Peichoto, María Elisa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Colubridae
Rear-Fanged Snake
Hemorrhage
Coagulation
Platelets
Dipsadidae
topic Colubridae
Rear-Fanged Snake
Hemorrhage
Coagulation
Platelets
Dipsadidae
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Philodryas baroni - an attractively colored snake - has become readily available through the exotic pet trade. Most people consider this species harmless; however, it has already caused human envenomation. As little is known about the venom from this South-American opisthoglyphous "colubrid" snake, herein, we studied its protein composition by SDS-PAGE, as well as its effects on the hemostatic system. Both reducing and nonreducing SDS-PAGE analysis demonstrated that the venom exhibits greatest complexity in the range of 50-80 kDa. The venom displayed proteolytic activity towards azocollagen, with a specific activity of 75.5 U/ mg, and rapidly hydrolyzed the Aa-chain of fibrinogen, exhibiting lower activity towards the Bb- and g-chains. Philodryas baroni venom showed no platelet pro-aggregating activity per se, but it inhibited collagen- and thrombin-induced platelet aggregation. Prominent hemorrhage developed in mouse skin after intradermal injection of the crude venom, and its minimum hemorrhagic dose was 13.9 mg. When injected intramuscularly into the gastrocnemius of mice, the venom induced local effects such as hemorrhage, myonecrosis, edema and leucocyte infiltration. Due to its venom toxicity shown herein, P. baroni should be considered dangerous to humans and any medically significant bite should be promptly reviewed by a qualified health professional.
Fil: Sanchez, Matias Nicolas. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; Argentina
Fil: Timoniuk, A.. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina
Fil: Maruñak, S.. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina
Fil: Teibler, P.. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina
Fil: Acosta, Ofelia Cristina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; Argentina
Fil: Peichoto, María Elisa. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; Argentina
description Philodryas baroni - an attractively colored snake - has become readily available through the exotic pet trade. Most people consider this species harmless; however, it has already caused human envenomation. As little is known about the venom from this South-American opisthoglyphous "colubrid" snake, herein, we studied its protein composition by SDS-PAGE, as well as its effects on the hemostatic system. Both reducing and nonreducing SDS-PAGE analysis demonstrated that the venom exhibits greatest complexity in the range of 50-80 kDa. The venom displayed proteolytic activity towards azocollagen, with a specific activity of 75.5 U/ mg, and rapidly hydrolyzed the Aa-chain of fibrinogen, exhibiting lower activity towards the Bb- and g-chains. Philodryas baroni venom showed no platelet pro-aggregating activity per se, but it inhibited collagen- and thrombin-induced platelet aggregation. Prominent hemorrhage developed in mouse skin after intradermal injection of the crude venom, and its minimum hemorrhagic dose was 13.9 mg. When injected intramuscularly into the gastrocnemius of mice, the venom induced local effects such as hemorrhage, myonecrosis, edema and leucocyte infiltration. Due to its venom toxicity shown herein, P. baroni should be considered dangerous to humans and any medically significant bite should be promptly reviewed by a qualified health professional.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8334
Sanchez, Matias Nicolas; Timoniuk, A.; Maruñak, S.; Teibler, P.; Acosta, Ofelia Cristina; et al.; Biochemical and biological analysis of Philodryas baroni (Baron´s Green Racer; Dipsadidae) venom: relevance of the findings to human risk assessment; Sage Publications Ltd; Human And Experimental Toxicoloxy; 33; 1; 6-2013; 22-31
1477-0903
0960-3271
url http://hdl.handle.net/11336/8334
identifier_str_mv Sanchez, Matias Nicolas; Timoniuk, A.; Maruñak, S.; Teibler, P.; Acosta, Ofelia Cristina; et al.; Biochemical and biological analysis of Philodryas baroni (Baron´s Green Racer; Dipsadidae) venom: relevance of the findings to human risk assessment; Sage Publications Ltd; Human And Experimental Toxicoloxy; 33; 1; 6-2013; 22-31
1477-0903
0960-3271
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://het.sagepub.com/content/33/1/22
info:eu-repo/semantics/altIdentifier/doi/10.1177/0960327113493302
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sage Publications Ltd
publisher.none.fl_str_mv Sage Publications Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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