Inflammatory effects of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venom

Autores
Peichoto, María Elisa; Zychar, Bianca Cestari; Tavares, Flávio Luiz; De Camargo Gonçalves, Luis Roberto; Acosta, Ofelia Cristina; Santoro, Marcelo Larami
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Patagonfibrase is a P-III class metalloproteinase isolated from the venom of Philodryas patagoniensis, a South-American, rear-fanged 'colubrid' snake responsible for accidents with clinical significance. Since local inflammatory reactions are conspicuous signs of snakebites inflicted by this species and taking into consideration that most snake venom metalloproteinases exhibit inflammatory activity, this study deals with the proinflammatory effects evoked by patagonfibrase. Herein, we demonstrate that patagonfibrase causes a time- and dose-dependent hemorrhagic edema when injected into mouse hind paws. The peak of edema occurred at 30 min after injection, and the minimum edematogenic dose was 0.021 μg. By histological analysis, the presence of moderate to marked edema and hemorrhage, and a mild inflammatory infiltrate was observed. When injected subcutaneously into the scrotal bag of mice, patagonfibrase induced cell recruitment with a significant alteration in physiological parameters of leukocyte-endothelium interaction. The presence of 1 mmol/L o-phenanthroline, which chelates metal ions, significantly inhibited the proinflammatory effects induced by patagonfibrase. Taken together, these results imply that patagonfibrase is an important contributor to local inflammation elicited by P. patagoniensis envenomation, which may pave the way for novel therapeutic strategies to treat this snakebite. Moreover, our findings demonstrate for the first time that a venom metalloproteinase from a rear-fanged snake elicits proinflammatory effects mainly mediated by its catalytic activity.
Fil: Peichoto, María Elisa. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zychar, Bianca Cestari. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; Brasil
Fil: Tavares, Flávio Luiz. Universidad de la Cuenca del Plata; Argentina
Fil: De Camargo Gonçalves, Luis Roberto. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; Brasil
Fil: Acosta, Ofelia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste; Argentina
Fil: Santoro, Marcelo Larami. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; Brasil
Materia
Colubridae
Edema
Leukocyte-Endothelium Interaction
Rear-Fanged Snake Venom
Toxin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43197
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/43197
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inflammatory effects of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venomPeichoto, María ElisaZychar, Bianca CestariTavares, Flávio LuizDe Camargo Gonçalves, Luis RobertoAcosta, Ofelia CristinaSantoro, Marcelo LaramiColubridaeEdemaLeukocyte-Endothelium InteractionRear-Fanged Snake VenomToxinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Patagonfibrase is a P-III class metalloproteinase isolated from the venom of Philodryas patagoniensis, a South-American, rear-fanged 'colubrid' snake responsible for accidents with clinical significance. Since local inflammatory reactions are conspicuous signs of snakebites inflicted by this species and taking into consideration that most snake venom metalloproteinases exhibit inflammatory activity, this study deals with the proinflammatory effects evoked by patagonfibrase. Herein, we demonstrate that patagonfibrase causes a time- and dose-dependent hemorrhagic edema when injected into mouse hind paws. The peak of edema occurred at 30 min after injection, and the minimum edematogenic dose was 0.021 μg. By histological analysis, the presence of moderate to marked edema and hemorrhage, and a mild inflammatory infiltrate was observed. When injected subcutaneously into the scrotal bag of mice, patagonfibrase induced cell recruitment with a significant alteration in physiological parameters of leukocyte-endothelium interaction. The presence of 1 mmol/L o-phenanthroline, which chelates metal ions, significantly inhibited the proinflammatory effects induced by patagonfibrase. Taken together, these results imply that patagonfibrase is an important contributor to local inflammation elicited by P. patagoniensis envenomation, which may pave the way for novel therapeutic strategies to treat this snakebite. Moreover, our findings demonstrate for the first time that a venom metalloproteinase from a rear-fanged snake elicits proinflammatory effects mainly mediated by its catalytic activity.Fil: Peichoto, María Elisa. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zychar, Bianca Cestari. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; BrasilFil: Tavares, Flávio Luiz. Universidad de la Cuenca del Plata; ArgentinaFil: De Camargo Gonçalves, Luis Roberto. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; BrasilFil: Acosta, Ofelia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste; ArgentinaFil: Santoro, Marcelo Larami. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; BrasilSoc Experimental Biology Medicine2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43197Peichoto, María Elisa; Zychar, Bianca Cestari; Tavares, Flávio Luiz; De Camargo Gonçalves, Luis Roberto; Acosta, Ofelia Cristina; et al.; Inflammatory effects of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venom; Soc Experimental Biology Medicine; Experimental Biology And Medicine; 236; 10; 10-2011; 1166-11721535-37021535-3699CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1258/ebm.2011.011125info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1258/ebm.2011.011125info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:50Zoai:ri.conicet.gov.ar:11336/43197instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:51.068CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inflammatory effects of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venom
title Inflammatory effects of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venom
spellingShingle Inflammatory effects of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venom
Peichoto, María Elisa
Colubridae
Edema
Leukocyte-Endothelium Interaction
Rear-Fanged Snake Venom
Toxin
title_short Inflammatory effects of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venom
title_full Inflammatory effects of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venom
title_fullStr Inflammatory effects of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venom
title_full_unstemmed Inflammatory effects of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venom
title_sort Inflammatory effects of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venom
dc.creator.none.fl_str_mv Peichoto, María Elisa
Zychar, Bianca Cestari
Tavares, Flávio Luiz
De Camargo Gonçalves, Luis Roberto
Acosta, Ofelia Cristina
Santoro, Marcelo Larami
author Peichoto, María Elisa
author_facet Peichoto, María Elisa
Zychar, Bianca Cestari
Tavares, Flávio Luiz
De Camargo Gonçalves, Luis Roberto
Acosta, Ofelia Cristina
Santoro, Marcelo Larami
author_role author
author2 Zychar, Bianca Cestari
Tavares, Flávio Luiz
De Camargo Gonçalves, Luis Roberto
Acosta, Ofelia Cristina
Santoro, Marcelo Larami
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Colubridae
Edema
Leukocyte-Endothelium Interaction
Rear-Fanged Snake Venom
Toxin
topic Colubridae
Edema
Leukocyte-Endothelium Interaction
Rear-Fanged Snake Venom
Toxin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Patagonfibrase is a P-III class metalloproteinase isolated from the venom of Philodryas patagoniensis, a South-American, rear-fanged 'colubrid' snake responsible for accidents with clinical significance. Since local inflammatory reactions are conspicuous signs of snakebites inflicted by this species and taking into consideration that most snake venom metalloproteinases exhibit inflammatory activity, this study deals with the proinflammatory effects evoked by patagonfibrase. Herein, we demonstrate that patagonfibrase causes a time- and dose-dependent hemorrhagic edema when injected into mouse hind paws. The peak of edema occurred at 30 min after injection, and the minimum edematogenic dose was 0.021 μg. By histological analysis, the presence of moderate to marked edema and hemorrhage, and a mild inflammatory infiltrate was observed. When injected subcutaneously into the scrotal bag of mice, patagonfibrase induced cell recruitment with a significant alteration in physiological parameters of leukocyte-endothelium interaction. The presence of 1 mmol/L o-phenanthroline, which chelates metal ions, significantly inhibited the proinflammatory effects induced by patagonfibrase. Taken together, these results imply that patagonfibrase is an important contributor to local inflammation elicited by P. patagoniensis envenomation, which may pave the way for novel therapeutic strategies to treat this snakebite. Moreover, our findings demonstrate for the first time that a venom metalloproteinase from a rear-fanged snake elicits proinflammatory effects mainly mediated by its catalytic activity.
Fil: Peichoto, María Elisa. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zychar, Bianca Cestari. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; Brasil
Fil: Tavares, Flávio Luiz. Universidad de la Cuenca del Plata; Argentina
Fil: De Camargo Gonçalves, Luis Roberto. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; Brasil
Fil: Acosta, Ofelia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste; Argentina
Fil: Santoro, Marcelo Larami. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; Brasil
description Patagonfibrase is a P-III class metalloproteinase isolated from the venom of Philodryas patagoniensis, a South-American, rear-fanged 'colubrid' snake responsible for accidents with clinical significance. Since local inflammatory reactions are conspicuous signs of snakebites inflicted by this species and taking into consideration that most snake venom metalloproteinases exhibit inflammatory activity, this study deals with the proinflammatory effects evoked by patagonfibrase. Herein, we demonstrate that patagonfibrase causes a time- and dose-dependent hemorrhagic edema when injected into mouse hind paws. The peak of edema occurred at 30 min after injection, and the minimum edematogenic dose was 0.021 μg. By histological analysis, the presence of moderate to marked edema and hemorrhage, and a mild inflammatory infiltrate was observed. When injected subcutaneously into the scrotal bag of mice, patagonfibrase induced cell recruitment with a significant alteration in physiological parameters of leukocyte-endothelium interaction. The presence of 1 mmol/L o-phenanthroline, which chelates metal ions, significantly inhibited the proinflammatory effects induced by patagonfibrase. Taken together, these results imply that patagonfibrase is an important contributor to local inflammation elicited by P. patagoniensis envenomation, which may pave the way for novel therapeutic strategies to treat this snakebite. Moreover, our findings demonstrate for the first time that a venom metalloproteinase from a rear-fanged snake elicits proinflammatory effects mainly mediated by its catalytic activity.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43197
Peichoto, María Elisa; Zychar, Bianca Cestari; Tavares, Flávio Luiz; De Camargo Gonçalves, Luis Roberto; Acosta, Ofelia Cristina; et al.; Inflammatory effects of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venom; Soc Experimental Biology Medicine; Experimental Biology And Medicine; 236; 10; 10-2011; 1166-1172
1535-3702
1535-3699
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43197
identifier_str_mv Peichoto, María Elisa; Zychar, Bianca Cestari; Tavares, Flávio Luiz; De Camargo Gonçalves, Luis Roberto; Acosta, Ofelia Cristina; et al.; Inflammatory effects of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venom; Soc Experimental Biology Medicine; Experimental Biology And Medicine; 236; 10; 10-2011; 1166-1172
1535-3702
1535-3699
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1258/ebm.2011.011125
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1258/ebm.2011.011125
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Soc Experimental Biology Medicine
publisher.none.fl_str_mv Soc Experimental Biology Medicine
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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