Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptin

Autores
Pérez Pérez, A.; Maymo, Julieta Lorena; Gambino, Yésica Paola; Guadix, P.; Dueñas, J. L.; Varone, Cecilia Laura; Sánchez Margalet, V.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Placentas from gestational diabetes (GDM) suff er from structural and functional changes including overgrowth. That is why we aimed to study [ 3 H]-leucine incorporation into protein in addition to translation signaling in placenta from GDM. Thus, we investigated the expression of leptin and leptin receptor (LEPR), as well as the activation state of signaling proteins regulating protein synthesis, such as mTOR, S6 Kinase, EIF4E-BP1, EIF4E, and eEF2 by measuring protein phosphorylation by immunoblot. [ 3 H]-Leucine incorporation into protein also was determined in trophoblastic placenta explants from GDM and control pregnancy. We found that leptin and LEPR expression are increased in placentas from GDM and the translation machinery activity as well as [ 3 H]-leucine incorporation into protein were higher in placentas from GDM compared with placentas from control pregnancy. In conclusion, protein synthesis rate is increased in placenta from GDM patients, and this may be due, at least in part, by the activation of translation signaling. The increased expression of leptin and LEPR may contribute to these eff ects. These results may provide a possible mechanism for the previously observed increase in placenta growth in GDM.
Fil: Pérez Pérez, A.. Universidad de Sevilla; España
Fil: Maymo, Julieta Lorena. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Gambino, Yésica Paola. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Guadix, P.. Universidad de Sevilla; España
Fil: Dueñas, J. L.. Universidad de Sevilla; España
Fil: Varone, Cecilia Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Sánchez Margalet, V.. Universidad de Sevilla; España
Materia
Leptin
Placenta
Insulin
Diabetes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21237
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptinPérez Pérez, A.Maymo, Julieta LorenaGambino, Yésica PaolaGuadix, P.Dueñas, J. L.Varone, Cecilia LauraSánchez Margalet, V.LeptinPlacentaInsulinDiabeteshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Placentas from gestational diabetes (GDM) suff er from structural and functional changes including overgrowth. That is why we aimed to study [ 3 H]-leucine incorporation into protein in addition to translation signaling in placenta from GDM. Thus, we investigated the expression of leptin and leptin receptor (LEPR), as well as the activation state of signaling proteins regulating protein synthesis, such as mTOR, S6 Kinase, EIF4E-BP1, EIF4E, and eEF2 by measuring protein phosphorylation by immunoblot. [ 3 H]-Leucine incorporation into protein also was determined in trophoblastic placenta explants from GDM and control pregnancy. We found that leptin and LEPR expression are increased in placentas from GDM and the translation machinery activity as well as [ 3 H]-leucine incorporation into protein were higher in placentas from GDM compared with placentas from control pregnancy. In conclusion, protein synthesis rate is increased in placenta from GDM patients, and this may be due, at least in part, by the activation of translation signaling. The increased expression of leptin and LEPR may contribute to these eff ects. These results may provide a possible mechanism for the previously observed increase in placenta growth in GDM.Fil: Pérez Pérez, A.. Universidad de Sevilla; EspañaFil: Maymo, Julieta Lorena. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Gambino, Yésica Paola. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Guadix, P.. Universidad de Sevilla; EspañaFil: Dueñas, J. L.. Universidad de Sevilla; EspañaFil: Varone, Cecilia Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Sánchez Margalet, V.. Universidad de Sevilla; EspañaGeorg Thieme Verlag Kg2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21237Pérez Pérez, A.; Maymo, Julieta Lorena; Gambino, Yésica Paola; Guadix, P.; Dueñas, J. L.; et al.; Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptin; Georg Thieme Verlag Kg; Hormone and Metabolic Research; 45; 6; 2-2013; 436-4420018-5043CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1055/s-0032-1333276info:eu-repo/semantics/altIdentifier/url/https://www.thieme-connect.de/DOI/DOI?10.1055/s-0032-1333276info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:07Zoai:ri.conicet.gov.ar:11336/21237instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:07.402CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptin
title Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptin
spellingShingle Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptin
Pérez Pérez, A.
Leptin
Placenta
Insulin
Diabetes
title_short Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptin
title_full Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptin
title_fullStr Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptin
title_full_unstemmed Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptin
title_sort Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptin
dc.creator.none.fl_str_mv Pérez Pérez, A.
Maymo, Julieta Lorena
Gambino, Yésica Paola
Guadix, P.
Dueñas, J. L.
Varone, Cecilia Laura
Sánchez Margalet, V.
author Pérez Pérez, A.
author_facet Pérez Pérez, A.
Maymo, Julieta Lorena
Gambino, Yésica Paola
Guadix, P.
Dueñas, J. L.
Varone, Cecilia Laura
Sánchez Margalet, V.
author_role author
author2 Maymo, Julieta Lorena
Gambino, Yésica Paola
Guadix, P.
Dueñas, J. L.
Varone, Cecilia Laura
Sánchez Margalet, V.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Leptin
Placenta
Insulin
Diabetes
topic Leptin
Placenta
Insulin
Diabetes
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Placentas from gestational diabetes (GDM) suff er from structural and functional changes including overgrowth. That is why we aimed to study [ 3 H]-leucine incorporation into protein in addition to translation signaling in placenta from GDM. Thus, we investigated the expression of leptin and leptin receptor (LEPR), as well as the activation state of signaling proteins regulating protein synthesis, such as mTOR, S6 Kinase, EIF4E-BP1, EIF4E, and eEF2 by measuring protein phosphorylation by immunoblot. [ 3 H]-Leucine incorporation into protein also was determined in trophoblastic placenta explants from GDM and control pregnancy. We found that leptin and LEPR expression are increased in placentas from GDM and the translation machinery activity as well as [ 3 H]-leucine incorporation into protein were higher in placentas from GDM compared with placentas from control pregnancy. In conclusion, protein synthesis rate is increased in placenta from GDM patients, and this may be due, at least in part, by the activation of translation signaling. The increased expression of leptin and LEPR may contribute to these eff ects. These results may provide a possible mechanism for the previously observed increase in placenta growth in GDM.
Fil: Pérez Pérez, A.. Universidad de Sevilla; España
Fil: Maymo, Julieta Lorena. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Gambino, Yésica Paola. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Guadix, P.. Universidad de Sevilla; España
Fil: Dueñas, J. L.. Universidad de Sevilla; España
Fil: Varone, Cecilia Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Sánchez Margalet, V.. Universidad de Sevilla; España
description Placentas from gestational diabetes (GDM) suff er from structural and functional changes including overgrowth. That is why we aimed to study [ 3 H]-leucine incorporation into protein in addition to translation signaling in placenta from GDM. Thus, we investigated the expression of leptin and leptin receptor (LEPR), as well as the activation state of signaling proteins regulating protein synthesis, such as mTOR, S6 Kinase, EIF4E-BP1, EIF4E, and eEF2 by measuring protein phosphorylation by immunoblot. [ 3 H]-Leucine incorporation into protein also was determined in trophoblastic placenta explants from GDM and control pregnancy. We found that leptin and LEPR expression are increased in placentas from GDM and the translation machinery activity as well as [ 3 H]-leucine incorporation into protein were higher in placentas from GDM compared with placentas from control pregnancy. In conclusion, protein synthesis rate is increased in placenta from GDM patients, and this may be due, at least in part, by the activation of translation signaling. The increased expression of leptin and LEPR may contribute to these eff ects. These results may provide a possible mechanism for the previously observed increase in placenta growth in GDM.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21237
Pérez Pérez, A.; Maymo, Julieta Lorena; Gambino, Yésica Paola; Guadix, P.; Dueñas, J. L.; et al.; Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptin; Georg Thieme Verlag Kg; Hormone and Metabolic Research; 45; 6; 2-2013; 436-442
0018-5043
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21237
identifier_str_mv Pérez Pérez, A.; Maymo, Julieta Lorena; Gambino, Yésica Paola; Guadix, P.; Dueñas, J. L.; et al.; Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptin; Georg Thieme Verlag Kg; Hormone and Metabolic Research; 45; 6; 2-2013; 436-442
0018-5043
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1055/s-0032-1333276
info:eu-repo/semantics/altIdentifier/url/https://www.thieme-connect.de/DOI/DOI?10.1055/s-0032-1333276
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Georg Thieme Verlag Kg
publisher.none.fl_str_mv Georg Thieme Verlag Kg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269620693630976
score 13.13397

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