Antineoplastic effect of the flavonoid quercetin in a kaposi͛´s sarcoma cellular model

Autores
Lezcano, Virginia Alicia; Principe, Gabriel; Tapia, Cinthia Mariela; Morelli, Susana; González Pardo, María Verónica
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Quercetin (QUE) is a flavonoid present in a wide variety of foods with different biological andpharmacological effects such as antitumor activity. Kaposi͛s sarcoma (KS) is a malignant Herpesvirusinduced tumor characterized by angiogenesis and proliferation of cells with characteristics of activatedendothelial cells. In this work we studied the antineoplastic effect of QUE and the modulation ofERK1/2, AKT and Wnt/ɴ-catenin signaling in a KS cellular model. Tumor cells were treated with QUE atdifferent concentrations (1-50 μM) for 24 and 48 h. Crystal violet staining revealed that QUEsignificantly decreases cell proliferation in a dose and time dependent manner: 77.8±5.1% 20 μM vs. Cand 85.7±7.5% 50 μM vs. C (24 h); 66.4±6.3% 10 μM vs. C, 55.9±2% 20 μM vs. C and 50.8±7.3 50 μMvs. C (48 h). In concordance, representative images showed an increase of cells with apoptoticcharacteristics. MTS assay demonstrated a significant decrease in cell viability at highest doses of QUE(84.4±7.1% 20 μM vs. C; 86±6.9% 50 μM vs. C) at 24 h and (63.7±7% 10 μM vs. C; 43.3±5.7% 20 μM vs.C; 34.3±7% vs. C) 48 h. Under the same experimental conditions, phosphorylated ERK1/2 and AKTwere analyzed by Western blot (WB) revealing an increment in their phosphorylation levels in a dosedependent way after 24 h of QUE. Since Wnt/ɴ-catenin signaling pathway play an important role intumor development and is activated in KS, ɴ-catenin protein levels were also analyzed by WB showingan increment of its expression from 5 μM of QUE. Altogether, these results demonstrate an antitumoreffect of QUE on KS cellular model, accompanied by ERK1/2 and AKT activation and an increase in ɴcatenin expression, a key protein of Wnt signaling pathway.
Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Principe, Gabriel. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tapia, Cinthia Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Morelli, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
XXXVI Reunión Científica Anual de la Asociación Argentina de Osteología y Metabolismo Mineral
Buenos Aires
Argentina
Society of Osteology and Mineral Metabolism
Materia
KAPOSI´S SARCOMA
QUERCETIN
APOPTOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/188125
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Antineoplastic effect of the flavonoid quercetin in a kaposi͛´s sarcoma cellular modelLezcano, Virginia AliciaPrincipe, GabrielTapia, Cinthia MarielaMorelli, SusanaGonzález Pardo, María VerónicaKAPOSI´S SARCOMAQUERCETINAPOPTOSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Quercetin (QUE) is a flavonoid present in a wide variety of foods with different biological andpharmacological effects such as antitumor activity. Kaposi͛s sarcoma (KS) is a malignant Herpesvirusinduced tumor characterized by angiogenesis and proliferation of cells with characteristics of activatedendothelial cells. In this work we studied the antineoplastic effect of QUE and the modulation ofERK1/2, AKT and Wnt/ɴ-catenin signaling in a KS cellular model. Tumor cells were treated with QUE atdifferent concentrations (1-50 μM) for 24 and 48 h. Crystal violet staining revealed that QUEsignificantly decreases cell proliferation in a dose and time dependent manner: 77.8±5.1% 20 μM vs. Cand 85.7±7.5% 50 μM vs. C (24 h); 66.4±6.3% 10 μM vs. C, 55.9±2% 20 μM vs. C and 50.8±7.3 50 μMvs. C (48 h). In concordance, representative images showed an increase of cells with apoptoticcharacteristics. MTS assay demonstrated a significant decrease in cell viability at highest doses of QUE(84.4±7.1% 20 μM vs. C; 86±6.9% 50 μM vs. C) at 24 h and (63.7±7% 10 μM vs. C; 43.3±5.7% 20 μM vs.C; 34.3±7% vs. C) 48 h. Under the same experimental conditions, phosphorylated ERK1/2 and AKTwere analyzed by Western blot (WB) revealing an increment in their phosphorylation levels in a dosedependent way after 24 h of QUE. Since Wnt/ɴ-catenin signaling pathway play an important role intumor development and is activated in KS, ɴ-catenin protein levels were also analyzed by WB showingan increment of its expression from 5 μM of QUE. Altogether, these results demonstrate an antitumoreffect of QUE on KS cellular model, accompanied by ERK1/2 and AKT activation and an increase in ɴcatenin expression, a key protein of Wnt signaling pathway.Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Principe, Gabriel. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tapia, Cinthia Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Morelli, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaXXXVI Reunión Científica Anual de la Asociación Argentina de Osteología y Metabolismo MineralBuenos AiresArgentinaSociety of Osteology and Mineral MetabolismWiley2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/188125Antineoplastic effect of the flavonoid quercetin in a kaposi͛´s sarcoma cellular model; XXXVI Reunión Científica Anual de la Asociación Argentina de Osteología y Metabolismo Mineral; Buenos Aires; Argentina; 2019; 4-42473-4039CONICET DigitalCONICETenghttps://aaomm.org.ar/congreso-2019/info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.1002/jbm4.10340info:eu-repo/semantics/altIdentifier/url/https://asbmr.onlinelibrary.wiley.com/toc/24734039/2020/4/S1Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:35Zoai:ri.conicet.gov.ar:11336/188125instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:35.397CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antineoplastic effect of the flavonoid quercetin in a kaposi͛´s sarcoma cellular model
title Antineoplastic effect of the flavonoid quercetin in a kaposi͛´s sarcoma cellular model
spellingShingle Antineoplastic effect of the flavonoid quercetin in a kaposi͛´s sarcoma cellular model
Lezcano, Virginia Alicia
KAPOSI´S SARCOMA
QUERCETIN
APOPTOSIS
title_short Antineoplastic effect of the flavonoid quercetin in a kaposi͛´s sarcoma cellular model
title_full Antineoplastic effect of the flavonoid quercetin in a kaposi͛´s sarcoma cellular model
title_fullStr Antineoplastic effect of the flavonoid quercetin in a kaposi͛´s sarcoma cellular model
title_full_unstemmed Antineoplastic effect of the flavonoid quercetin in a kaposi͛´s sarcoma cellular model
title_sort Antineoplastic effect of the flavonoid quercetin in a kaposi͛´s sarcoma cellular model
dc.creator.none.fl_str_mv Lezcano, Virginia Alicia
Principe, Gabriel
Tapia, Cinthia Mariela
Morelli, Susana
González Pardo, María Verónica
author Lezcano, Virginia Alicia
author_facet Lezcano, Virginia Alicia
Principe, Gabriel
Tapia, Cinthia Mariela
Morelli, Susana
González Pardo, María Verónica
author_role author
author2 Principe, Gabriel
Tapia, Cinthia Mariela
Morelli, Susana
González Pardo, María Verónica
author2_role author
author
author
author
dc.subject.none.fl_str_mv KAPOSI´S SARCOMA
QUERCETIN
APOPTOSIS
topic KAPOSI´S SARCOMA
QUERCETIN
APOPTOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Quercetin (QUE) is a flavonoid present in a wide variety of foods with different biological andpharmacological effects such as antitumor activity. Kaposi͛s sarcoma (KS) is a malignant Herpesvirusinduced tumor characterized by angiogenesis and proliferation of cells with characteristics of activatedendothelial cells. In this work we studied the antineoplastic effect of QUE and the modulation ofERK1/2, AKT and Wnt/ɴ-catenin signaling in a KS cellular model. Tumor cells were treated with QUE atdifferent concentrations (1-50 μM) for 24 and 48 h. Crystal violet staining revealed that QUEsignificantly decreases cell proliferation in a dose and time dependent manner: 77.8±5.1% 20 μM vs. Cand 85.7±7.5% 50 μM vs. C (24 h); 66.4±6.3% 10 μM vs. C, 55.9±2% 20 μM vs. C and 50.8±7.3 50 μMvs. C (48 h). In concordance, representative images showed an increase of cells with apoptoticcharacteristics. MTS assay demonstrated a significant decrease in cell viability at highest doses of QUE(84.4±7.1% 20 μM vs. C; 86±6.9% 50 μM vs. C) at 24 h and (63.7±7% 10 μM vs. C; 43.3±5.7% 20 μM vs.C; 34.3±7% vs. C) 48 h. Under the same experimental conditions, phosphorylated ERK1/2 and AKTwere analyzed by Western blot (WB) revealing an increment in their phosphorylation levels in a dosedependent way after 24 h of QUE. Since Wnt/ɴ-catenin signaling pathway play an important role intumor development and is activated in KS, ɴ-catenin protein levels were also analyzed by WB showingan increment of its expression from 5 μM of QUE. Altogether, these results demonstrate an antitumoreffect of QUE on KS cellular model, accompanied by ERK1/2 and AKT activation and an increase in ɴcatenin expression, a key protein of Wnt signaling pathway.
Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Principe, Gabriel. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tapia, Cinthia Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Morelli, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
XXXVI Reunión Científica Anual de la Asociación Argentina de Osteología y Metabolismo Mineral
Buenos Aires
Argentina
Society of Osteology and Mineral Metabolism
description Quercetin (QUE) is a flavonoid present in a wide variety of foods with different biological andpharmacological effects such as antitumor activity. Kaposi͛s sarcoma (KS) is a malignant Herpesvirusinduced tumor characterized by angiogenesis and proliferation of cells with characteristics of activatedendothelial cells. In this work we studied the antineoplastic effect of QUE and the modulation ofERK1/2, AKT and Wnt/ɴ-catenin signaling in a KS cellular model. Tumor cells were treated with QUE atdifferent concentrations (1-50 μM) for 24 and 48 h. Crystal violet staining revealed that QUEsignificantly decreases cell proliferation in a dose and time dependent manner: 77.8±5.1% 20 μM vs. Cand 85.7±7.5% 50 μM vs. C (24 h); 66.4±6.3% 10 μM vs. C, 55.9±2% 20 μM vs. C and 50.8±7.3 50 μMvs. C (48 h). In concordance, representative images showed an increase of cells with apoptoticcharacteristics. MTS assay demonstrated a significant decrease in cell viability at highest doses of QUE(84.4±7.1% 20 μM vs. C; 86±6.9% 50 μM vs. C) at 24 h and (63.7±7% 10 μM vs. C; 43.3±5.7% 20 μM vs.C; 34.3±7% vs. C) 48 h. Under the same experimental conditions, phosphorylated ERK1/2 and AKTwere analyzed by Western blot (WB) revealing an increment in their phosphorylation levels in a dosedependent way after 24 h of QUE. Since Wnt/ɴ-catenin signaling pathway play an important role intumor development and is activated in KS, ɴ-catenin protein levels were also analyzed by WB showingan increment of its expression from 5 μM of QUE. Altogether, these results demonstrate an antitumoreffect of QUE on KS cellular model, accompanied by ERK1/2 and AKT activation and an increase in ɴcatenin expression, a key protein of Wnt signaling pathway.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/188125
Antineoplastic effect of the flavonoid quercetin in a kaposi͛´s sarcoma cellular model; XXXVI Reunión Científica Anual de la Asociación Argentina de Osteología y Metabolismo Mineral; Buenos Aires; Argentina; 2019; 4-4
2473-4039
CONICET Digital
CONICET
url http://hdl.handle.net/11336/188125
identifier_str_mv Antineoplastic effect of the flavonoid quercetin in a kaposi͛´s sarcoma cellular model; XXXVI Reunión Científica Anual de la Asociación Argentina de Osteología y Metabolismo Mineral; Buenos Aires; Argentina; 2019; 4-4
2473-4039
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://aaomm.org.ar/congreso-2019/
info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.1002/jbm4.10340
info:eu-repo/semantics/altIdentifier/url/https://asbmr.onlinelibrary.wiley.com/toc/24734039/2020/4/S1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
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