An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria

Autores
Endres, Stephan; Wingen, Marcus; Torra, Joaquim; Ruiz González, Rubén; Polen, Tino; Bosio, Gabriela Natalia; Bitzenhofer, Nora Lisa; Hilgers, Fabienne; Gensch, Thomas; Nonell, Santi; Jaeger, Karl-Erich; Drepper, Thomas
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Flavin-binding fluorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efficacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate 1O2, all of the other tested flavoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable differences in ROS selectivity and yield. Accordingly, most LOV-FPs are potent photosensitizers, which can be used for light-controlled killing of bacteria. Furthermore, the two variants Pp2FbFP and DsFbFP M49I, exhibiting preferential photosensitization of singlet oxygen or singlet oxygen and superoxide, respectively, were shown to be new tools for studying specific ROS-induced cell signaling processes. The tested LOV-FPs thus further expand the toolbox of optogenetic sensitizers usable for a broad spectrum of microbiological and biomedical applications.
Fil: Endres, Stephan. Universitat Dusseldorf; Alemania
Fil: Wingen, Marcus. Universitat Dusseldorf; Alemania
Fil: Torra, Joaquim. Universitat Ramon Llull; España
Fil: Ruiz González, Rubén. Universitat Ramon Llull; España
Fil: Polen, Tino. Forschungszentrum Jülich; Alemania
Fil: Bosio, Gabriela Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Bitzenhofer, Nora Lisa. Universitat Dusseldorf; Alemania
Fil: Hilgers, Fabienne. Universitat Dusseldorf; Alemania
Fil: Gensch, Thomas. Forschungszentrum Jülich; Alemania
Fil: Nonell, Santi. Universitat Ramon Llull; España
Fil: Jaeger, Karl-Erich. Universitat Dusseldorf; Alemania
Fil: Drepper, Thomas. Forschungszentrum Jülich; Alemania
Materia
OPTOGENETICA
FOTOSENSIBILIZADOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102185
Acceder
id CONICETDig_3a9775692c45db4d2362ee650e87fc17
oai_identifier_str oai:ri.conicet.gov.ar:11336/102185
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteriaEndres, StephanWingen, MarcusTorra, JoaquimRuiz González, RubénPolen, TinoBosio, Gabriela NataliaBitzenhofer, Nora LisaHilgers, FabienneGensch, ThomasNonell, SantiJaeger, Karl-ErichDrepper, ThomasOPTOGENETICAFOTOSENSIBILIZADORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Flavin-binding fluorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efficacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate 1O2, all of the other tested flavoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable differences in ROS selectivity and yield. Accordingly, most LOV-FPs are potent photosensitizers, which can be used for light-controlled killing of bacteria. Furthermore, the two variants Pp2FbFP and DsFbFP M49I, exhibiting preferential photosensitization of singlet oxygen or singlet oxygen and superoxide, respectively, were shown to be new tools for studying specific ROS-induced cell signaling processes. The tested LOV-FPs thus further expand the toolbox of optogenetic sensitizers usable for a broad spectrum of microbiological and biomedical applications.Fil: Endres, Stephan. Universitat Dusseldorf; AlemaniaFil: Wingen, Marcus. Universitat Dusseldorf; AlemaniaFil: Torra, Joaquim. Universitat Ramon Llull; EspañaFil: Ruiz González, Rubén. Universitat Ramon Llull; EspañaFil: Polen, Tino. Forschungszentrum Jülich; AlemaniaFil: Bosio, Gabriela Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Bitzenhofer, Nora Lisa. Universitat Dusseldorf; AlemaniaFil: Hilgers, Fabienne. Universitat Dusseldorf; AlemaniaFil: Gensch, Thomas. Forschungszentrum Jülich; AlemaniaFil: Nonell, Santi. Universitat Ramon Llull; EspañaFil: Jaeger, Karl-Erich. Universitat Dusseldorf; AlemaniaFil: Drepper, Thomas. Forschungszentrum Jülich; AlemaniaSpringer2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102185Endres, Stephan; Wingen, Marcus; Torra, Joaquim; Ruiz González, Rubén; Polen, Tino; et al.; An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria; Springer; Scientific Reports; 8; 1; 10-20182045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-018-33291-4info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-33291-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:38:08Zoai:ri.conicet.gov.ar:11336/102185instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:38:08.522CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
spellingShingle An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
Endres, Stephan
OPTOGENETICA
FOTOSENSIBILIZADOR
title_short An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_full An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_fullStr An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_full_unstemmed An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_sort An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
dc.creator.none.fl_str_mv Endres, Stephan
Wingen, Marcus
Torra, Joaquim
Ruiz González, Rubén
Polen, Tino
Bosio, Gabriela Natalia
Bitzenhofer, Nora Lisa
Hilgers, Fabienne
Gensch, Thomas
Nonell, Santi
Jaeger, Karl-Erich
Drepper, Thomas
author Endres, Stephan
author_facet Endres, Stephan
Wingen, Marcus
Torra, Joaquim
Ruiz González, Rubén
Polen, Tino
Bosio, Gabriela Natalia
Bitzenhofer, Nora Lisa
Hilgers, Fabienne
Gensch, Thomas
Nonell, Santi
Jaeger, Karl-Erich
Drepper, Thomas
author_role author
author2 Wingen, Marcus
Torra, Joaquim
Ruiz González, Rubén
Polen, Tino
Bosio, Gabriela Natalia
Bitzenhofer, Nora Lisa
Hilgers, Fabienne
Gensch, Thomas
Nonell, Santi
Jaeger, Karl-Erich
Drepper, Thomas
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv OPTOGENETICA
FOTOSENSIBILIZADOR
topic OPTOGENETICA
FOTOSENSIBILIZADOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Flavin-binding fluorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efficacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate 1O2, all of the other tested flavoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable differences in ROS selectivity and yield. Accordingly, most LOV-FPs are potent photosensitizers, which can be used for light-controlled killing of bacteria. Furthermore, the two variants Pp2FbFP and DsFbFP M49I, exhibiting preferential photosensitization of singlet oxygen or singlet oxygen and superoxide, respectively, were shown to be new tools for studying specific ROS-induced cell signaling processes. The tested LOV-FPs thus further expand the toolbox of optogenetic sensitizers usable for a broad spectrum of microbiological and biomedical applications.
Fil: Endres, Stephan. Universitat Dusseldorf; Alemania
Fil: Wingen, Marcus. Universitat Dusseldorf; Alemania
Fil: Torra, Joaquim. Universitat Ramon Llull; España
Fil: Ruiz González, Rubén. Universitat Ramon Llull; España
Fil: Polen, Tino. Forschungszentrum Jülich; Alemania
Fil: Bosio, Gabriela Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Bitzenhofer, Nora Lisa. Universitat Dusseldorf; Alemania
Fil: Hilgers, Fabienne. Universitat Dusseldorf; Alemania
Fil: Gensch, Thomas. Forschungszentrum Jülich; Alemania
Fil: Nonell, Santi. Universitat Ramon Llull; España
Fil: Jaeger, Karl-Erich. Universitat Dusseldorf; Alemania
Fil: Drepper, Thomas. Forschungszentrum Jülich; Alemania
description Flavin-binding fluorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efficacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate 1O2, all of the other tested flavoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable differences in ROS selectivity and yield. Accordingly, most LOV-FPs are potent photosensitizers, which can be used for light-controlled killing of bacteria. Furthermore, the two variants Pp2FbFP and DsFbFP M49I, exhibiting preferential photosensitization of singlet oxygen or singlet oxygen and superoxide, respectively, were shown to be new tools for studying specific ROS-induced cell signaling processes. The tested LOV-FPs thus further expand the toolbox of optogenetic sensitizers usable for a broad spectrum of microbiological and biomedical applications.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102185
Endres, Stephan; Wingen, Marcus; Torra, Joaquim; Ruiz González, Rubén; Polen, Tino; et al.; An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria; Springer; Scientific Reports; 8; 1; 10-2018
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102185
identifier_str_mv Endres, Stephan; Wingen, Marcus; Torra, Joaquim; Ruiz González, Rubén; Polen, Tino; et al.; An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria; Springer; Scientific Reports; 8; 1; 10-2018
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-018-33291-4
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-33291-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782035608207360
score 12.982451

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