An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria

Autores
Endres, Stephan; Wingen, Marcus; Torra, Joaquim; Ruiz González, Rubén; Polen, Tino; Bosio, Gabriela Natalia; Bitzenhofer, Nora Lisa; Hilgers, Fabienne; Gensch, Thomas; Nonell, Santi; Jaeger, Karl-Erich; Drepper, Thomas
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Flavin-binding fuorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efcacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate 1O2, all of the other tested favoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable diferences in ROS selectivity and yield. Accordingly, most LOV-FPs are potent photosensitizers, which can be used for light-controlled killing of bacteria. Furthermore, the two variants Pp2FbFP and DsFbFP M49I, exhibiting preferential photosensitization of singlet oxygen or singlet oxygen and superoxide, respectively, were shown to be new tools for studying specifc ROS-induced cell signaling processes. The tested LOV-FPs thus further expand the toolbox of optogenetic sensitizers usable for a broad spectrum of microbiological and biomedical applications.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Materia
Química
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/108133
Acceder
id SEDICI_2006909ba467412e3049df3a99a9982e
oai_identifier_str oai:sedici.unlp.edu.ar:10915/108133
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteriaEndres, StephanWingen, MarcusTorra, JoaquimRuiz González, RubénPolen, TinoBosio, Gabriela NataliaBitzenhofer, Nora LisaHilgers, FabienneGensch, ThomasNonell, SantiJaeger, Karl-ErichDrepper, ThomasQuímicaFlavin-binding fuorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efcacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate 1O2, all of the other tested favoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable diferences in ROS selectivity and yield. Accordingly, most LOV-FPs are potent photosensitizers, which can be used for light-controlled killing of bacteria. Furthermore, the two variants Pp2FbFP and DsFbFP M49I, exhibiting preferential photosensitization of singlet oxygen or singlet oxygen and superoxide, respectively, were shown to be new tools for studying specifc ROS-induced cell signaling processes. The tested LOV-FPs thus further expand the toolbox of optogenetic sensitizers usable for a broad spectrum of microbiological and biomedical applications.Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/108133enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6177443&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/issn/2045-2322info:eu-repo/semantics/altIdentifier/pmid/30301917info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-33291-4info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:04:47Zoai:sedici.unlp.edu.ar:10915/108133Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:04:48.216SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
spellingShingle An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
Endres, Stephan
Química
title_short An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_full An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_fullStr An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_full_unstemmed An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_sort An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
dc.creator.none.fl_str_mv Endres, Stephan
Wingen, Marcus
Torra, Joaquim
Ruiz González, Rubén
Polen, Tino
Bosio, Gabriela Natalia
Bitzenhofer, Nora Lisa
Hilgers, Fabienne
Gensch, Thomas
Nonell, Santi
Jaeger, Karl-Erich
Drepper, Thomas
author Endres, Stephan
author_facet Endres, Stephan
Wingen, Marcus
Torra, Joaquim
Ruiz González, Rubén
Polen, Tino
Bosio, Gabriela Natalia
Bitzenhofer, Nora Lisa
Hilgers, Fabienne
Gensch, Thomas
Nonell, Santi
Jaeger, Karl-Erich
Drepper, Thomas
author_role author
author2 Wingen, Marcus
Torra, Joaquim
Ruiz González, Rubén
Polen, Tino
Bosio, Gabriela Natalia
Bitzenhofer, Nora Lisa
Hilgers, Fabienne
Gensch, Thomas
Nonell, Santi
Jaeger, Karl-Erich
Drepper, Thomas
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Química
topic Química
dc.description.none.fl_txt_mv Flavin-binding fuorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efcacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate 1O2, all of the other tested favoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable diferences in ROS selectivity and yield. Accordingly, most LOV-FPs are potent photosensitizers, which can be used for light-controlled killing of bacteria. Furthermore, the two variants Pp2FbFP and DsFbFP M49I, exhibiting preferential photosensitization of singlet oxygen or singlet oxygen and superoxide, respectively, were shown to be new tools for studying specifc ROS-induced cell signaling processes. The tested LOV-FPs thus further expand the toolbox of optogenetic sensitizers usable for a broad spectrum of microbiological and biomedical applications.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
description Flavin-binding fuorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efcacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate 1O2, all of the other tested favoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable diferences in ROS selectivity and yield. Accordingly, most LOV-FPs are potent photosensitizers, which can be used for light-controlled killing of bacteria. Furthermore, the two variants Pp2FbFP and DsFbFP M49I, exhibiting preferential photosensitization of singlet oxygen or singlet oxygen and superoxide, respectively, were shown to be new tools for studying specifc ROS-induced cell signaling processes. The tested LOV-FPs thus further expand the toolbox of optogenetic sensitizers usable for a broad spectrum of microbiological and biomedical applications.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/108133
url http://sedici.unlp.edu.ar/handle/10915/108133
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6177443&blobtype=pdf
info:eu-repo/semantics/altIdentifier/issn/2045-2322
info:eu-repo/semantics/altIdentifier/pmid/30301917
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-33291-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1846783324855468032
score 12.982451

Publicaciones similares