Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detection

Autores
Wang, Chien Chun; Sombra, Lorena Luján; Fernandez, Liliana Patricia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, Amberlite XAD-1180 resin is used for on-line surfactant-mediated pre-concentration of sildenafil as a prior step for its fluorescent detection. In order to activate the column for sildenafil pre-concentration, the cationic surfactant (hexadecyltrimethylammoniunm bromide, HTAB) is adsorbed onto the resin. In these conditions, sildenafil is retained by HTAB–resin and then it is eluted with ethanol and analyzed by spectrofluorimetry. Drug–surfactant association produces a considerable fluorescence enhancement, increasing considerably the sensitivity of detection. Therefore, sildenafil can be pre-concentrated and quantitatively determined, with a detection limit of 0.2 ng mL1 . The proposed method was successfully applied to the analysis of bulk drug, human urine, tablets, and local herbal medicine. Validation processes were performed by recovering studies and statistical analysis with satisfactory results.
Fil: Wang, Chien Chun. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Sombra, Lorena Luján. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Materia
On-Line Preconcentration
Fia-Fluorescence
Polymeric Resin
Surfactant
Sildenafil
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14491
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/14491
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detectionWang, Chien ChunSombra, Lorena LujánFernandez, Liliana PatriciaOn-Line PreconcentrationFia-FluorescencePolymeric ResinSurfactantSildenafilhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this work, Amberlite XAD-1180 resin is used for on-line surfactant-mediated pre-concentration of sildenafil as a prior step for its fluorescent detection. In order to activate the column for sildenafil pre-concentration, the cationic surfactant (hexadecyltrimethylammoniunm bromide, HTAB) is adsorbed onto the resin. In these conditions, sildenafil is retained by HTAB–resin and then it is eluted with ethanol and analyzed by spectrofluorimetry. Drug–surfactant association produces a considerable fluorescence enhancement, increasing considerably the sensitivity of detection. Therefore, sildenafil can be pre-concentrated and quantitatively determined, with a detection limit of 0.2 ng mL1 . The proposed method was successfully applied to the analysis of bulk drug, human urine, tablets, and local herbal medicine. Validation processes were performed by recovering studies and statistical analysis with satisfactory results.Fil: Wang, Chien Chun. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Sombra, Lorena Luján. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Fernandez, Liliana Patricia. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaElsevier Science2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14491Wang, Chien Chun; Sombra, Lorena Luján; Fernandez, Liliana Patricia; Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detection; Elsevier Science; Talanta; 98; 8-2012; 247-2520039-9140enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2012.07.003info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0039914012005516info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:28Zoai:ri.conicet.gov.ar:11336/14491instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:28.509CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detection
title Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detection
spellingShingle Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detection
Wang, Chien Chun
On-Line Preconcentration
Fia-Fluorescence
Polymeric Resin
Surfactant
Sildenafil
title_short Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detection
title_full Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detection
title_fullStr Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detection
title_full_unstemmed Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detection
title_sort Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detection
dc.creator.none.fl_str_mv Wang, Chien Chun
Sombra, Lorena Luján
Fernandez, Liliana Patricia
author Wang, Chien Chun
author_facet Wang, Chien Chun
Sombra, Lorena Luján
Fernandez, Liliana Patricia
author_role author
author2 Sombra, Lorena Luján
Fernandez, Liliana Patricia
author2_role author
author
dc.subject.none.fl_str_mv On-Line Preconcentration
Fia-Fluorescence
Polymeric Resin
Surfactant
Sildenafil
topic On-Line Preconcentration
Fia-Fluorescence
Polymeric Resin
Surfactant
Sildenafil
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work, Amberlite XAD-1180 resin is used for on-line surfactant-mediated pre-concentration of sildenafil as a prior step for its fluorescent detection. In order to activate the column for sildenafil pre-concentration, the cationic surfactant (hexadecyltrimethylammoniunm bromide, HTAB) is adsorbed onto the resin. In these conditions, sildenafil is retained by HTAB–resin and then it is eluted with ethanol and analyzed by spectrofluorimetry. Drug–surfactant association produces a considerable fluorescence enhancement, increasing considerably the sensitivity of detection. Therefore, sildenafil can be pre-concentrated and quantitatively determined, with a detection limit of 0.2 ng mL1 . The proposed method was successfully applied to the analysis of bulk drug, human urine, tablets, and local herbal medicine. Validation processes were performed by recovering studies and statistical analysis with satisfactory results.
Fil: Wang, Chien Chun. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Sombra, Lorena Luján. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
description In this work, Amberlite XAD-1180 resin is used for on-line surfactant-mediated pre-concentration of sildenafil as a prior step for its fluorescent detection. In order to activate the column for sildenafil pre-concentration, the cationic surfactant (hexadecyltrimethylammoniunm bromide, HTAB) is adsorbed onto the resin. In these conditions, sildenafil is retained by HTAB–resin and then it is eluted with ethanol and analyzed by spectrofluorimetry. Drug–surfactant association produces a considerable fluorescence enhancement, increasing considerably the sensitivity of detection. Therefore, sildenafil can be pre-concentrated and quantitatively determined, with a detection limit of 0.2 ng mL1 . The proposed method was successfully applied to the analysis of bulk drug, human urine, tablets, and local herbal medicine. Validation processes were performed by recovering studies and statistical analysis with satisfactory results.
publishDate 2012
dc.date.none.fl_str_mv 2012-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14491
Wang, Chien Chun; Sombra, Lorena Luján; Fernandez, Liliana Patricia; Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detection; Elsevier Science; Talanta; 98; 8-2012; 247-252
0039-9140
url http://hdl.handle.net/11336/14491
identifier_str_mv Wang, Chien Chun; Sombra, Lorena Luján; Fernandez, Liliana Patricia; Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detection; Elsevier Science; Talanta; 98; 8-2012; 247-252
0039-9140
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2012.07.003
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0039914012005516
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269227511185408
score 13.13397

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