Capillary electrophoresis for glutathione and glutathione disulfide quantification to monitor oxidative stress by cadmium in Glycine max L. leaves

Autores
Felici, Carlos Emiliano; Acosta, Maria Gimena; Perez Chaca, Verónica; Molina, Alicia; Randazzo, G.; Marsa, Silvana; Zirulnik, Fanny; Gomez, Maria Roxana Anabel
Año de publicación
2011
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Antioxidant defense systems allow plants to develop a tolerance to contamination with cadmium (Cd). A rapid and accurate capillary electrophoresis (CE) method for the simultaneous determination of GSH and GSSG in Glycine max L. leaves was developed. The method was applied to monitor the oxidative stress after Cd adding. Soybean seeds were germinated under controlled conditions. On the 4th day were placed in hydroponic conditions, with Hoagland nutrient solution. On the 10th day were subjected to Cd poisoning (40 μM) for 4, 6, 24, 72 h and 6 days. To obtain extracts, 250 mg of leaves were homogenized under ice-cold conditions in HCl 0.5M. Homogenates were centrifuged for 10 min and the supernatants were analyzed. A Beckman P/ACE MDQ instrument equipped with a DAD and P/ACE System MDQ Software was used. Linear relationships were obtained between corrected peak areas and concentrations of the analytes. Detection was performed at 214 and 198 nm GSH and GSSG were baseline separated in less than 6 min, the corresponding migration times were 4.97 and 5.41 min, respectively. Along the time curve GSH levels were higher than in the respective controls, while GSSG was always lower. GSSG content in the analyzed samples was low and GSH content was high. However, both compounds could be separated and quantified fast and accurately without interference. The increased levels of GSH showed that the antioxidant mechanisms in the plant are working, defending it, and presenting a level of tolerance to Cd stress.
Fil: Felici, Carlos Emiliano. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Perez Chaca, Verónica. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Molina, Alicia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Randazzo, G.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Marsa, Silvana. Universidad Nacional de San Luis; Argentina
Fil: Zirulnik, Fanny. Universidad Nacional de San Luis; Argentina
Fil: Gomez, Maria Roxana Anabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
XXVIII Annual Scientific Meeting
Argentina
Sociedad de Biologia de Cuyo
Materia
capillary electrophoresis
glutathione
oxidative stress
Glycine max L.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243271
Acceder
id CONICETDig_384fc858fc0a3751afbd3aafdbc5e5b8
oai_identifier_str oai:ri.conicet.gov.ar:11336/243271
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Capillary electrophoresis for glutathione and glutathione disulfide quantification to monitor oxidative stress by cadmium in Glycine max L. leavesFelici, Carlos EmilianoAcosta, Maria GimenaPerez Chaca, VerónicaMolina, AliciaRandazzo, G.Marsa, SilvanaZirulnik, FannyGomez, Maria Roxana Anabelcapillary electrophoresisglutathioneoxidative stressGlycine max L.https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Antioxidant defense systems allow plants to develop a tolerance to contamination with cadmium (Cd). A rapid and accurate capillary electrophoresis (CE) method for the simultaneous determination of GSH and GSSG in Glycine max L. leaves was developed. The method was applied to monitor the oxidative stress after Cd adding. Soybean seeds were germinated under controlled conditions. On the 4th day were placed in hydroponic conditions, with Hoagland nutrient solution. On the 10th day were subjected to Cd poisoning (40 μM) for 4, 6, 24, 72 h and 6 days. To obtain extracts, 250 mg of leaves were homogenized under ice-cold conditions in HCl 0.5M. Homogenates were centrifuged for 10 min and the supernatants were analyzed. A Beckman P/ACE MDQ instrument equipped with a DAD and P/ACE System MDQ Software was used. Linear relationships were obtained between corrected peak areas and concentrations of the analytes. Detection was performed at 214 and 198 nm GSH and GSSG were baseline separated in less than 6 min, the corresponding migration times were 4.97 and 5.41 min, respectively. Along the time curve GSH levels were higher than in the respective controls, while GSSG was always lower. GSSG content in the analyzed samples was low and GSH content was high. However, both compounds could be separated and quantified fast and accurately without interference. The increased levels of GSH showed that the antioxidant mechanisms in the plant are working, defending it, and presenting a level of tolerance to Cd stress.Fil: Felici, Carlos Emiliano. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Perez Chaca, Verónica. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Molina, Alicia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Randazzo, G.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Marsa, Silvana. Universidad Nacional de San Luis; ArgentinaFil: Zirulnik, Fanny. Universidad Nacional de San Luis; ArgentinaFil: Gomez, Maria Roxana Anabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaXXVIII Annual Scientific MeetingArgentinaSociedad de Biologia de CuyoInstituto de Histología y Embriología2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/243271Capillary electrophoresis for glutathione and glutathione disulfide quantification to monitor oxidative stress by cadmium in Glycine max L. leaves; XXVIII Annual Scientific Meeting; Argentina; 2011; 16-160327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/reuniones-anuales-anteriores/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:15:10Zoai:ri.conicet.gov.ar:11336/243271instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:15:10.475CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Capillary electrophoresis for glutathione and glutathione disulfide quantification to monitor oxidative stress by cadmium in Glycine max L. leaves
title Capillary electrophoresis for glutathione and glutathione disulfide quantification to monitor oxidative stress by cadmium in Glycine max L. leaves
spellingShingle Capillary electrophoresis for glutathione and glutathione disulfide quantification to monitor oxidative stress by cadmium in Glycine max L. leaves
Felici, Carlos Emiliano
capillary electrophoresis
glutathione
oxidative stress
Glycine max L.
title_short Capillary electrophoresis for glutathione and glutathione disulfide quantification to monitor oxidative stress by cadmium in Glycine max L. leaves
title_full Capillary electrophoresis for glutathione and glutathione disulfide quantification to monitor oxidative stress by cadmium in Glycine max L. leaves
title_fullStr Capillary electrophoresis for glutathione and glutathione disulfide quantification to monitor oxidative stress by cadmium in Glycine max L. leaves
title_full_unstemmed Capillary electrophoresis for glutathione and glutathione disulfide quantification to monitor oxidative stress by cadmium in Glycine max L. leaves
title_sort Capillary electrophoresis for glutathione and glutathione disulfide quantification to monitor oxidative stress by cadmium in Glycine max L. leaves
dc.creator.none.fl_str_mv Felici, Carlos Emiliano
Acosta, Maria Gimena
Perez Chaca, Verónica
Molina, Alicia
Randazzo, G.
Marsa, Silvana
Zirulnik, Fanny
Gomez, Maria Roxana Anabel
author Felici, Carlos Emiliano
author_facet Felici, Carlos Emiliano
Acosta, Maria Gimena
Perez Chaca, Verónica
Molina, Alicia
Randazzo, G.
Marsa, Silvana
Zirulnik, Fanny
Gomez, Maria Roxana Anabel
author_role author
author2 Acosta, Maria Gimena
Perez Chaca, Verónica
Molina, Alicia
Randazzo, G.
Marsa, Silvana
Zirulnik, Fanny
Gomez, Maria Roxana Anabel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv capillary electrophoresis
glutathione
oxidative stress
Glycine max L.
topic capillary electrophoresis
glutathione
oxidative stress
Glycine max L.
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Antioxidant defense systems allow plants to develop a tolerance to contamination with cadmium (Cd). A rapid and accurate capillary electrophoresis (CE) method for the simultaneous determination of GSH and GSSG in Glycine max L. leaves was developed. The method was applied to monitor the oxidative stress after Cd adding. Soybean seeds were germinated under controlled conditions. On the 4th day were placed in hydroponic conditions, with Hoagland nutrient solution. On the 10th day were subjected to Cd poisoning (40 μM) for 4, 6, 24, 72 h and 6 days. To obtain extracts, 250 mg of leaves were homogenized under ice-cold conditions in HCl 0.5M. Homogenates were centrifuged for 10 min and the supernatants were analyzed. A Beckman P/ACE MDQ instrument equipped with a DAD and P/ACE System MDQ Software was used. Linear relationships were obtained between corrected peak areas and concentrations of the analytes. Detection was performed at 214 and 198 nm GSH and GSSG were baseline separated in less than 6 min, the corresponding migration times were 4.97 and 5.41 min, respectively. Along the time curve GSH levels were higher than in the respective controls, while GSSG was always lower. GSSG content in the analyzed samples was low and GSH content was high. However, both compounds could be separated and quantified fast and accurately without interference. The increased levels of GSH showed that the antioxidant mechanisms in the plant are working, defending it, and presenting a level of tolerance to Cd stress.
Fil: Felici, Carlos Emiliano. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Perez Chaca, Verónica. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Molina, Alicia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Randazzo, G.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Marsa, Silvana. Universidad Nacional de San Luis; Argentina
Fil: Zirulnik, Fanny. Universidad Nacional de San Luis; Argentina
Fil: Gomez, Maria Roxana Anabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
XXVIII Annual Scientific Meeting
Argentina
Sociedad de Biologia de Cuyo
description Antioxidant defense systems allow plants to develop a tolerance to contamination with cadmium (Cd). A rapid and accurate capillary electrophoresis (CE) method for the simultaneous determination of GSH and GSSG in Glycine max L. leaves was developed. The method was applied to monitor the oxidative stress after Cd adding. Soybean seeds were germinated under controlled conditions. On the 4th day were placed in hydroponic conditions, with Hoagland nutrient solution. On the 10th day were subjected to Cd poisoning (40 μM) for 4, 6, 24, 72 h and 6 days. To obtain extracts, 250 mg of leaves were homogenized under ice-cold conditions in HCl 0.5M. Homogenates were centrifuged for 10 min and the supernatants were analyzed. A Beckman P/ACE MDQ instrument equipped with a DAD and P/ACE System MDQ Software was used. Linear relationships were obtained between corrected peak areas and concentrations of the analytes. Detection was performed at 214 and 198 nm GSH and GSSG were baseline separated in less than 6 min, the corresponding migration times were 4.97 and 5.41 min, respectively. Along the time curve GSH levels were higher than in the respective controls, while GSSG was always lower. GSSG content in the analyzed samples was low and GSH content was high. However, both compounds could be separated and quantified fast and accurately without interference. The increased levels of GSH showed that the antioxidant mechanisms in the plant are working, defending it, and presenting a level of tolerance to Cd stress.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243271
Capillary electrophoresis for glutathione and glutathione disulfide quantification to monitor oxidative stress by cadmium in Glycine max L. leaves; XXVIII Annual Scientific Meeting; Argentina; 2011; 16-16
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243271
identifier_str_mv Capillary electrophoresis for glutathione and glutathione disulfide quantification to monitor oxidative stress by cadmium in Glycine max L. leaves; XXVIII Annual Scientific Meeting; Argentina; 2011; 16-16
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/reuniones-anuales-anteriores/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/msword
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología
publisher.none.fl_str_mv Instituto de Histología y Embriología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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