Critical evaluation of buffering solutions for p K a determination by capillary electrophoresis

Autores
Fuguet, Elisabet; Reta, Mario Roberto; Gibert, Carme; Rosés, Martí; Bosch, Elisabeth; Ràfols, Clara
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The performance of the most common and also some other less common CE buffers has been tested for the pK determination of several types of compounds (pyridine, amines, and phenols). The selected buffers cover a pH ranging from 3.7 to 11.8. Whereas some buffers, like acetic acid/acetate, BisTrisH/BisTris, TrisH/Tris, CHES/CHES and CAPS/CAPS can be used with all type of analytes, others like ammonium/ammonia, butylammonium/butylammonia, ethylammonium/ethylammonia, diethylammonium/diethylammonia, and hydrogenphosphate/phosphate are not recommended because they interact with a wide range of compounds. The rest of the tested buffers (dihydrogenphosphate/hydrogenphosphate,MES/MES , HEPES/HEPES, and boric acid/borate) can show specific interactions depending on the nature of the analytes, and their use in some applications should be restricted.
Fil: Fuguet, Elisabet. Universidad de Barcelona; España
Fil: Reta, Mario Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Gibert, Carme. Universidad de Barcelona; España
Fil: Rosés, Martí. Universidad de Barcelona; España
Fil: Bosch, Elisabeth. Universidad de Barcelona; España
Fil: Ràfols, Clara. Universidad de Barcelona; España
Materia
capillary electrophoresis
pKa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242064
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Critical evaluation of buffering solutions for p K a determination by capillary electrophoresisFuguet, ElisabetReta, Mario RobertoGibert, CarmeRosés, MartíBosch, ElisabethRàfols, Claracapillary electrophoresispKahttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The performance of the most common and also some other less common CE buffers has been tested for the pK determination of several types of compounds (pyridine, amines, and phenols). The selected buffers cover a pH ranging from 3.7 to 11.8. Whereas some buffers, like acetic acid/acetate, BisTrisH/BisTris, TrisH/Tris, CHES/CHES and CAPS/CAPS can be used with all type of analytes, others like ammonium/ammonia, butylammonium/butylammonia, ethylammonium/ethylammonia, diethylammonium/diethylammonia, and hydrogenphosphate/phosphate are not recommended because they interact with a wide range of compounds. The rest of the tested buffers (dihydrogenphosphate/hydrogenphosphate,MES/MES , HEPES/HEPES, and boric acid/borate) can show specific interactions depending on the nature of the analytes, and their use in some applications should be restricted.Fil: Fuguet, Elisabet. Universidad de Barcelona; EspañaFil: Reta, Mario Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Gibert, Carme. Universidad de Barcelona; EspañaFil: Rosés, Martí. Universidad de Barcelona; EspañaFil: Bosch, Elisabeth. Universidad de Barcelona; EspañaFil: Ràfols, Clara. Universidad de Barcelona; EspañaWiley VCH Verlag2008-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242064Fuguet, Elisabet; Reta, Mario Roberto; Gibert, Carme; Rosés, Martí; Bosch, Elisabeth; et al.; Critical evaluation of buffering solutions for p K a determination by capillary electrophoresis; Wiley VCH Verlag; Electrophoresis; 29; 13; 4-2008; 2841-28510173-0835CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.200700869info:eu-repo/semantics/altIdentifier/doi/10.1002/elps.200700869info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:06Zoai:ri.conicet.gov.ar:11336/242064instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:06.547CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Critical evaluation of buffering solutions for p K a determination by capillary electrophoresis
title Critical evaluation of buffering solutions for p K a determination by capillary electrophoresis
spellingShingle Critical evaluation of buffering solutions for p K a determination by capillary electrophoresis
Fuguet, Elisabet
capillary electrophoresis
pKa
title_short Critical evaluation of buffering solutions for p K a determination by capillary electrophoresis
title_full Critical evaluation of buffering solutions for p K a determination by capillary electrophoresis
title_fullStr Critical evaluation of buffering solutions for p K a determination by capillary electrophoresis
title_full_unstemmed Critical evaluation of buffering solutions for p K a determination by capillary electrophoresis
title_sort Critical evaluation of buffering solutions for p K a determination by capillary electrophoresis
dc.creator.none.fl_str_mv Fuguet, Elisabet
Reta, Mario Roberto
Gibert, Carme
Rosés, Martí
Bosch, Elisabeth
Ràfols, Clara
author Fuguet, Elisabet
author_facet Fuguet, Elisabet
Reta, Mario Roberto
Gibert, Carme
Rosés, Martí
Bosch, Elisabeth
Ràfols, Clara
author_role author
author2 Reta, Mario Roberto
Gibert, Carme
Rosés, Martí
Bosch, Elisabeth
Ràfols, Clara
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv capillary electrophoresis
pKa
topic capillary electrophoresis
pKa
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The performance of the most common and also some other less common CE buffers has been tested for the pK determination of several types of compounds (pyridine, amines, and phenols). The selected buffers cover a pH ranging from 3.7 to 11.8. Whereas some buffers, like acetic acid/acetate, BisTrisH/BisTris, TrisH/Tris, CHES/CHES and CAPS/CAPS can be used with all type of analytes, others like ammonium/ammonia, butylammonium/butylammonia, ethylammonium/ethylammonia, diethylammonium/diethylammonia, and hydrogenphosphate/phosphate are not recommended because they interact with a wide range of compounds. The rest of the tested buffers (dihydrogenphosphate/hydrogenphosphate,MES/MES , HEPES/HEPES, and boric acid/borate) can show specific interactions depending on the nature of the analytes, and their use in some applications should be restricted.
Fil: Fuguet, Elisabet. Universidad de Barcelona; España
Fil: Reta, Mario Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Gibert, Carme. Universidad de Barcelona; España
Fil: Rosés, Martí. Universidad de Barcelona; España
Fil: Bosch, Elisabeth. Universidad de Barcelona; España
Fil: Ràfols, Clara. Universidad de Barcelona; España
description The performance of the most common and also some other less common CE buffers has been tested for the pK determination of several types of compounds (pyridine, amines, and phenols). The selected buffers cover a pH ranging from 3.7 to 11.8. Whereas some buffers, like acetic acid/acetate, BisTrisH/BisTris, TrisH/Tris, CHES/CHES and CAPS/CAPS can be used with all type of analytes, others like ammonium/ammonia, butylammonium/butylammonia, ethylammonium/ethylammonia, diethylammonium/diethylammonia, and hydrogenphosphate/phosphate are not recommended because they interact with a wide range of compounds. The rest of the tested buffers (dihydrogenphosphate/hydrogenphosphate,MES/MES , HEPES/HEPES, and boric acid/borate) can show specific interactions depending on the nature of the analytes, and their use in some applications should be restricted.
publishDate 2008
dc.date.none.fl_str_mv 2008-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242064
Fuguet, Elisabet; Reta, Mario Roberto; Gibert, Carme; Rosés, Martí; Bosch, Elisabeth; et al.; Critical evaluation of buffering solutions for p K a determination by capillary electrophoresis; Wiley VCH Verlag; Electrophoresis; 29; 13; 4-2008; 2841-2851
0173-0835
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242064
identifier_str_mv Fuguet, Elisabet; Reta, Mario Roberto; Gibert, Carme; Rosés, Martí; Bosch, Elisabeth; et al.; Critical evaluation of buffering solutions for p K a determination by capillary electrophoresis; Wiley VCH Verlag; Electrophoresis; 29; 13; 4-2008; 2841-2851
0173-0835
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.200700869
info:eu-repo/semantics/altIdentifier/doi/10.1002/elps.200700869
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270069293318144
score 13.13397

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