Simultaneous Separation of Ergot Alkaloids by Capillary Electrophoresis After Cloud Point Extraction From Cereal Samples

Autores
Felici, Carlos Emiliano; Wang, Chien Chun; Fernandez, Liliana Patricia; Gomez, Maria Roxana Anabel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new and sensitive analytical methodology for ergot alkaloids determination from cereal samples based on Cloud Point Extraction prior to Capillary Electrophoresis-UV absorbance was developed. The methodology involves extraction under acid conditions and subsequent preconcentration by applying a simple, rapid and environmentally friendly low volume surfactant extraction procedure. After extraction, CE analysis was carried out by performing dilutions on preconcentrated surfactant rich phase, achieving a single peak or simultaneous alkaloids determination. A real preconcentration factor of 22 of total ergot alkaloids was obtained, demonstrating the efficiency of this methodology. The limits of detection were 2.6 and 2.2 μg/kg for ergotamine and ergonovine, respectively. Validationvprocedure revealed suitable linearity, accuracy and precision. The average extraction andvclean-up recoveries were compared with the theoretical values and were better than 92%.This method was successfully applied to the determination of ergot alkaloids in different varieties of commercial flour samples, two grain samples and one of the leading brands cereal based product for infant feeding. The high sensitivity achieved for ergot alkaloids determinations in real samples suggests CPE procedure as an interesting approach to improve CE-UV visible detection limits. Moreover, the whole process could be considered as a contribution to green chemistry because non-organic solvents were involved, demonstrating its great potential over conventional techniques.
Fil: Felici, Carlos Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Wang, Chien Chun. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Gomez, Maria Roxana Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Materia
Ergot Alkaloids
Ergotamine
Capillary Electrophoresis
Cereals
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5784
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/5784
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Simultaneous Separation of Ergot Alkaloids by Capillary Electrophoresis After Cloud Point Extraction From Cereal SamplesFelici, Carlos EmilianoWang, Chien ChunFernandez, Liliana PatriciaGomez, Maria Roxana AnabelErgot AlkaloidsErgotamineCapillary ElectrophoresisCerealshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A new and sensitive analytical methodology for ergot alkaloids determination from cereal samples based on Cloud Point Extraction prior to Capillary Electrophoresis-UV absorbance was developed. The methodology involves extraction under acid conditions and subsequent preconcentration by applying a simple, rapid and environmentally friendly low volume surfactant extraction procedure. After extraction, CE analysis was carried out by performing dilutions on preconcentrated surfactant rich phase, achieving a single peak or simultaneous alkaloids determination. A real preconcentration factor of 22 of total ergot alkaloids was obtained, demonstrating the efficiency of this methodology. The limits of detection were 2.6 and 2.2 μg/kg for ergotamine and ergonovine, respectively. Validationvprocedure revealed suitable linearity, accuracy and precision. The average extraction andvclean-up recoveries were compared with the theoretical values and were better than 92%.This method was successfully applied to the determination of ergot alkaloids in different varieties of commercial flour samples, two grain samples and one of the leading brands cereal based product for infant feeding. The high sensitivity achieved for ergot alkaloids determinations in real samples suggests CPE procedure as an interesting approach to improve CE-UV visible detection limits. Moreover, the whole process could be considered as a contribution to green chemistry because non-organic solvents were involved, demonstrating its great potential over conventional techniques.Fil: Felici, Carlos Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Wang, Chien Chun. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Gomez, Maria Roxana Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaWiley2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5784Felici, Carlos Emiliano; Wang, Chien Chun; Fernandez, Liliana Patricia; Gomez, Maria Roxana Anabel; Simultaneous Separation of Ergot Alkaloids by Capillary Electrophoresis After Cloud Point Extraction From Cereal Samples; Wiley; Electrophoresis; 36; 2; 1-2015; 341-3470173-0835enginfo:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201400215info:eu-repo/semantics/altIdentifier/purl/http://onlinelibrary.wiley.com/doi/10.1002/elps.201400215/fullinfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:45:31Zoai:ri.conicet.gov.ar:11336/5784instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:45:31.923CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Simultaneous Separation of Ergot Alkaloids by Capillary Electrophoresis After Cloud Point Extraction From Cereal Samples
title Simultaneous Separation of Ergot Alkaloids by Capillary Electrophoresis After Cloud Point Extraction From Cereal Samples
spellingShingle Simultaneous Separation of Ergot Alkaloids by Capillary Electrophoresis After Cloud Point Extraction From Cereal Samples
Felici, Carlos Emiliano
Ergot Alkaloids
Ergotamine
Capillary Electrophoresis
Cereals
title_short Simultaneous Separation of Ergot Alkaloids by Capillary Electrophoresis After Cloud Point Extraction From Cereal Samples
title_full Simultaneous Separation of Ergot Alkaloids by Capillary Electrophoresis After Cloud Point Extraction From Cereal Samples
title_fullStr Simultaneous Separation of Ergot Alkaloids by Capillary Electrophoresis After Cloud Point Extraction From Cereal Samples
title_full_unstemmed Simultaneous Separation of Ergot Alkaloids by Capillary Electrophoresis After Cloud Point Extraction From Cereal Samples
title_sort Simultaneous Separation of Ergot Alkaloids by Capillary Electrophoresis After Cloud Point Extraction From Cereal Samples
dc.creator.none.fl_str_mv Felici, Carlos Emiliano
Wang, Chien Chun
Fernandez, Liliana Patricia
Gomez, Maria Roxana Anabel
author Felici, Carlos Emiliano
author_facet Felici, Carlos Emiliano
Wang, Chien Chun
Fernandez, Liliana Patricia
Gomez, Maria Roxana Anabel
author_role author
author2 Wang, Chien Chun
Fernandez, Liliana Patricia
Gomez, Maria Roxana Anabel
author2_role author
author
author
dc.subject.none.fl_str_mv Ergot Alkaloids
Ergotamine
Capillary Electrophoresis
Cereals
topic Ergot Alkaloids
Ergotamine
Capillary Electrophoresis
Cereals
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A new and sensitive analytical methodology for ergot alkaloids determination from cereal samples based on Cloud Point Extraction prior to Capillary Electrophoresis-UV absorbance was developed. The methodology involves extraction under acid conditions and subsequent preconcentration by applying a simple, rapid and environmentally friendly low volume surfactant extraction procedure. After extraction, CE analysis was carried out by performing dilutions on preconcentrated surfactant rich phase, achieving a single peak or simultaneous alkaloids determination. A real preconcentration factor of 22 of total ergot alkaloids was obtained, demonstrating the efficiency of this methodology. The limits of detection were 2.6 and 2.2 μg/kg for ergotamine and ergonovine, respectively. Validationvprocedure revealed suitable linearity, accuracy and precision. The average extraction andvclean-up recoveries were compared with the theoretical values and were better than 92%.This method was successfully applied to the determination of ergot alkaloids in different varieties of commercial flour samples, two grain samples and one of the leading brands cereal based product for infant feeding. The high sensitivity achieved for ergot alkaloids determinations in real samples suggests CPE procedure as an interesting approach to improve CE-UV visible detection limits. Moreover, the whole process could be considered as a contribution to green chemistry because non-organic solvents were involved, demonstrating its great potential over conventional techniques.
Fil: Felici, Carlos Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Wang, Chien Chun. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Gomez, Maria Roxana Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
description A new and sensitive analytical methodology for ergot alkaloids determination from cereal samples based on Cloud Point Extraction prior to Capillary Electrophoresis-UV absorbance was developed. The methodology involves extraction under acid conditions and subsequent preconcentration by applying a simple, rapid and environmentally friendly low volume surfactant extraction procedure. After extraction, CE analysis was carried out by performing dilutions on preconcentrated surfactant rich phase, achieving a single peak or simultaneous alkaloids determination. A real preconcentration factor of 22 of total ergot alkaloids was obtained, demonstrating the efficiency of this methodology. The limits of detection were 2.6 and 2.2 μg/kg for ergotamine and ergonovine, respectively. Validationvprocedure revealed suitable linearity, accuracy and precision. The average extraction andvclean-up recoveries were compared with the theoretical values and were better than 92%.This method was successfully applied to the determination of ergot alkaloids in different varieties of commercial flour samples, two grain samples and one of the leading brands cereal based product for infant feeding. The high sensitivity achieved for ergot alkaloids determinations in real samples suggests CPE procedure as an interesting approach to improve CE-UV visible detection limits. Moreover, the whole process could be considered as a contribution to green chemistry because non-organic solvents were involved, demonstrating its great potential over conventional techniques.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5784
Felici, Carlos Emiliano; Wang, Chien Chun; Fernandez, Liliana Patricia; Gomez, Maria Roxana Anabel; Simultaneous Separation of Ergot Alkaloids by Capillary Electrophoresis After Cloud Point Extraction From Cereal Samples; Wiley; Electrophoresis; 36; 2; 1-2015; 341-347
0173-0835
url http://hdl.handle.net/11336/5784
identifier_str_mv Felici, Carlos Emiliano; Wang, Chien Chun; Fernandez, Liliana Patricia; Gomez, Maria Roxana Anabel; Simultaneous Separation of Ergot Alkaloids by Capillary Electrophoresis After Cloud Point Extraction From Cereal Samples; Wiley; Electrophoresis; 36; 2; 1-2015; 341-347
0173-0835
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201400215
info:eu-repo/semantics/altIdentifier/purl/http://onlinelibrary.wiley.com/doi/10.1002/elps.201400215/full
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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