Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.

Autores
Felici, Carlos Emiliano; Molina, Alicia; Almeida, César Américo; Fernández Baldo, Martín Alejandro; Zirulnik, Fanny; Gomez, Maria Roxana Anabel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To analyze the effect of Cadmium (Cd) on the metabolism of reduced glutathione (GSH) in roots and leaves of Glycine max L. Methods: A capillary electrophoresis methodology was optimized to determinate simultaneously GSH and reduced and oxidized GSH in a precise and accurate way. The functional role of the genes involved in GSH cellular metabolism (γ-glutamylcysteine synthetase), GSH synthetase and glutathione reductase (GR) was evaluated. Finally, the activities of antioxidant enzymes as GR and superoxide dismutases were determinate. Results: The studies of γ-glutamylcysteine synthetase and GSH synthetase gene expression showed an increase and GR showed a decrease in Cd-treated plants. GR and superoxide dismutases activities increased at 24 h and 6 h respectively in roots under Cd exposure. GSH content was higher and showed a significant increase in roots and leaves at 6 h and 24 h of treatment. Conclusions: The results of the present study showed a better understanding in signaling pathway by alterations in antioxidant mechanisms by Cd in soybean seedlings.
Fil: Felici, Carlos Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Molina, Alicia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Almeida, César Américo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Zirulnik, Fanny. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Gomez, Maria Roxana Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Materia
Soybean
Cadmium Toxicity
Oxidative Stress
Antioxidant Defense
Glutathione
Capillary Electrophoresis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5707
Acceder
id CONICETDig_a1493a0434ce9fa3b0d9854b759ac8a0
oai_identifier_str oai:ri.conicet.gov.ar:11336/5707
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.Felici, Carlos EmilianoMolina, AliciaAlmeida, César AméricoFernández Baldo, Martín AlejandroZirulnik, FannyGomez, Maria Roxana AnabelSoybeanCadmium ToxicityOxidative StressAntioxidant DefenseGlutathioneCapillary Electrophoresishttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Objective: To analyze the effect of Cadmium (Cd) on the metabolism of reduced glutathione (GSH) in roots and leaves of Glycine max L. Methods: A capillary electrophoresis methodology was optimized to determinate simultaneously GSH and reduced and oxidized GSH in a precise and accurate way. The functional role of the genes involved in GSH cellular metabolism (γ-glutamylcysteine synthetase), GSH synthetase and glutathione reductase (GR) was evaluated. Finally, the activities of antioxidant enzymes as GR and superoxide dismutases were determinate. Results: The studies of γ-glutamylcysteine synthetase and GSH synthetase gene expression showed an increase and GR showed a decrease in Cd-treated plants. GR and superoxide dismutases activities increased at 24 h and 6 h respectively in roots under Cd exposure. GSH content was higher and showed a significant increase in roots and leaves at 6 h and 24 h of treatment. Conclusions: The results of the present study showed a better understanding in signaling pathway by alterations in antioxidant mechanisms by Cd in soybean seedlings.Fil: Felici, Carlos Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Molina, Alicia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Almeida, César Américo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis; ArgentinaFil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis; ArgentinaFil: Zirulnik, Fanny. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Gomez, Maria Roxana Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaHainan Medical University2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5707Felici, Carlos Emiliano; Molina, Alicia; Almeida, César Américo; Fernández Baldo, Martín Alejandro; Zirulnik, Fanny; et al.; Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.; Hainan Medical University; Journal of Coastal Life Medicine; 2; 10; 6-2014; 791-7982309-61522309-5288enginfo:eu-repo/semantics/altIdentifier/doi/10.12980/JCLM.2.2014JCLM-2014-0077info:eu-repo/semantics/altIdentifier/url/http://www.jclmm.com/qk/201410/6.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:42Zoai:ri.conicet.gov.ar:11336/5707instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:43.06CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.
title Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.
spellingShingle Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.
Felici, Carlos Emiliano
Soybean
Cadmium Toxicity
Oxidative Stress
Antioxidant Defense
Glutathione
Capillary Electrophoresis
title_short Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.
title_full Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.
title_fullStr Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.
title_full_unstemmed Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.
title_sort Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.
dc.creator.none.fl_str_mv Felici, Carlos Emiliano
Molina, Alicia
Almeida, César Américo
Fernández Baldo, Martín Alejandro
Zirulnik, Fanny
Gomez, Maria Roxana Anabel
author Felici, Carlos Emiliano
author_facet Felici, Carlos Emiliano
Molina, Alicia
Almeida, César Américo
Fernández Baldo, Martín Alejandro
Zirulnik, Fanny
Gomez, Maria Roxana Anabel
author_role author
author2 Molina, Alicia
Almeida, César Américo
Fernández Baldo, Martín Alejandro
Zirulnik, Fanny
Gomez, Maria Roxana Anabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Soybean
Cadmium Toxicity
Oxidative Stress
Antioxidant Defense
Glutathione
Capillary Electrophoresis
topic Soybean
Cadmium Toxicity
Oxidative Stress
Antioxidant Defense
Glutathione
Capillary Electrophoresis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective: To analyze the effect of Cadmium (Cd) on the metabolism of reduced glutathione (GSH) in roots and leaves of Glycine max L. Methods: A capillary electrophoresis methodology was optimized to determinate simultaneously GSH and reduced and oxidized GSH in a precise and accurate way. The functional role of the genes involved in GSH cellular metabolism (γ-glutamylcysteine synthetase), GSH synthetase and glutathione reductase (GR) was evaluated. Finally, the activities of antioxidant enzymes as GR and superoxide dismutases were determinate. Results: The studies of γ-glutamylcysteine synthetase and GSH synthetase gene expression showed an increase and GR showed a decrease in Cd-treated plants. GR and superoxide dismutases activities increased at 24 h and 6 h respectively in roots under Cd exposure. GSH content was higher and showed a significant increase in roots and leaves at 6 h and 24 h of treatment. Conclusions: The results of the present study showed a better understanding in signaling pathway by alterations in antioxidant mechanisms by Cd in soybean seedlings.
Fil: Felici, Carlos Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Molina, Alicia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Almeida, César Américo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Zirulnik, Fanny. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Gomez, Maria Roxana Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
description Objective: To analyze the effect of Cadmium (Cd) on the metabolism of reduced glutathione (GSH) in roots and leaves of Glycine max L. Methods: A capillary electrophoresis methodology was optimized to determinate simultaneously GSH and reduced and oxidized GSH in a precise and accurate way. The functional role of the genes involved in GSH cellular metabolism (γ-glutamylcysteine synthetase), GSH synthetase and glutathione reductase (GR) was evaluated. Finally, the activities of antioxidant enzymes as GR and superoxide dismutases were determinate. Results: The studies of γ-glutamylcysteine synthetase and GSH synthetase gene expression showed an increase and GR showed a decrease in Cd-treated plants. GR and superoxide dismutases activities increased at 24 h and 6 h respectively in roots under Cd exposure. GSH content was higher and showed a significant increase in roots and leaves at 6 h and 24 h of treatment. Conclusions: The results of the present study showed a better understanding in signaling pathway by alterations in antioxidant mechanisms by Cd in soybean seedlings.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5707
Felici, Carlos Emiliano; Molina, Alicia; Almeida, César Américo; Fernández Baldo, Martín Alejandro; Zirulnik, Fanny; et al.; Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.; Hainan Medical University; Journal of Coastal Life Medicine; 2; 10; 6-2014; 791-798
2309-6152
2309-5288
url http://hdl.handle.net/11336/5707
identifier_str_mv Felici, Carlos Emiliano; Molina, Alicia; Almeida, César Américo; Fernández Baldo, Martín Alejandro; Zirulnik, Fanny; et al.; Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.; Hainan Medical University; Journal of Coastal Life Medicine; 2; 10; 6-2014; 791-798
2309-6152
2309-5288
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.12980/JCLM.2.2014JCLM-2014-0077
info:eu-repo/semantics/altIdentifier/url/http://www.jclmm.com/qk/201410/6.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hainan Medical University
publisher.none.fl_str_mv Hainan Medical University
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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