Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons

Autores
Garelli, Andres; Heredia, Fabiana; Volonté, Yanel Andrea; Pereirinha, Joana; Casimiro, Andrea; Viegas, Filipe; Belém, Claudia; Tanaka, Kohtaro; Cardoso, Gisela; Macedo, Andre; Leal, Ana; Kotowicz, Malwina; Prado Spalm, Facundo Heber; Mendes, César; Gontijo, Alisson M.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Higher dipterans undergo metamorphosis within a puparium, a protective capsule made up of the reshaped and hardened cuticle of the last larval instar. Puparium formation (pupariation) in Drosophila starts when wandering stage larvae reduce their locomotion and initiate increasingly strong whole body contractions that together with the internalization of the three anteriormost body segments remodel the body and cuticle, reducing their length/width ratio from ~5 to ~3. This behavior lasts 5-10 min and culminates with the extrusion of a proteinaceous mix (glue) produced by the salivary glands. The animal then slowly moves forward in a caterpillar-like fashion, traveling about half its length for ~1 min to its final pupariation site. We call this behavior glue-spreading behavior (GSB), as it helps to spread the glue over the ventral part of the animal, promoting its attachment to the underlying substrate. Following GSB, the final shape of the puparium is set, even though weak and periodic contractions occur over the next 40-50 min (post-GSB), the operculum becomes defined, and the cuticle (i.e., the future puparium) starts to gradually sclerotize and tan. Here, we show that proper progression through three pupariation behaviors (pre-GSB, GSB, and postGSB) requires the Dilp8-Lgr3 pathway, a relaxin-like pathway that has been previously implicated in controlling the timing of pupariation in animals carrying aberrantly growing imaginal discs. During pupariation, however, Dilp8-Lgr3 signaling is spatiallyand temporally-distinct: a strong, epidermis-derived dilp8 expression peak that starts at the pre-GSB phase of pupariation appears to signal via Lgr3 in a novel subpopulation of thoracic CNS neurons. While this signaling ensures partial progression through the preGSB phase, and total progression through GSB and post-GSB phases, it is not required for sclerotization and tanning. These results demonstrate a new transient epidermis to neuron signaling event that facilitates progression through the cascade of pupariationassociated behaviors.
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; Portugal
Fil: Heredia, Fabiana. Universidade Nova de Lisboa; Portugal
Fil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; Portugal
Fil: Pereirinha, Joana. Universidade Nova de Lisboa; Portugal
Fil: Casimiro, Andrea. Universidade Nova de Lisboa; Portugal
Fil: Viegas, Filipe. Universidade Nova de Lisboa; Portugal
Fil: Belém, Claudia. Universidade Nova de Lisboa; Portugal
Fil: Tanaka, Kohtaro. Instituto Gulbenkian de Ciências; Portugal
Fil: Cardoso, Gisela. Universidade Nova de Lisboa; Portugal. Universidade de Sao Paulo; Brasil
Fil: Macedo, Andre. Universidade Nova de Lisboa; Portugal
Fil: Leal, Ana. Universidade Nova de Lisboa; Portugal
Fil: Kotowicz, Malwina. Universidade Nova de Lisboa; Portugal
Fil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Mendes, César. Universidade Nova de Lisboa; Portugal
Fil: Gontijo, Alisson M.. Universidade Nova de Lisboa; Portugal
Insect Hormones 2019
Kolymbari
Grecia
Bashirullah, Arash
Leopold, Pierre
Materia
DROSOPHILA
DILP8
LGR3
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194589
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/194589
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neuronsGarelli, AndresHeredia, FabianaVolonté, Yanel AndreaPereirinha, JoanaCasimiro, AndreaViegas, FilipeBelém, ClaudiaTanaka, KohtaroCardoso, GiselaMacedo, AndreLeal, AnaKotowicz, MalwinaPrado Spalm, Facundo HeberMendes, CésarGontijo, Alisson M.DROSOPHILADILP8LGR3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Higher dipterans undergo metamorphosis within a puparium, a protective capsule made up of the reshaped and hardened cuticle of the last larval instar. Puparium formation (pupariation) in Drosophila starts when wandering stage larvae reduce their locomotion and initiate increasingly strong whole body contractions that together with the internalization of the three anteriormost body segments remodel the body and cuticle, reducing their length/width ratio from ~5 to ~3. This behavior lasts 5-10 min and culminates with the extrusion of a proteinaceous mix (glue) produced by the salivary glands. The animal then slowly moves forward in a caterpillar-like fashion, traveling about half its length for ~1 min to its final pupariation site. We call this behavior glue-spreading behavior (GSB), as it helps to spread the glue over the ventral part of the animal, promoting its attachment to the underlying substrate. Following GSB, the final shape of the puparium is set, even though weak and periodic contractions occur over the next 40-50 min (post-GSB), the operculum becomes defined, and the cuticle (i.e., the future puparium) starts to gradually sclerotize and tan. Here, we show that proper progression through three pupariation behaviors (pre-GSB, GSB, and postGSB) requires the Dilp8-Lgr3 pathway, a relaxin-like pathway that has been previously implicated in controlling the timing of pupariation in animals carrying aberrantly growing imaginal discs. During pupariation, however, Dilp8-Lgr3 signaling is spatiallyand temporally-distinct: a strong, epidermis-derived dilp8 expression peak that starts at the pre-GSB phase of pupariation appears to signal via Lgr3 in a novel subpopulation of thoracic CNS neurons. While this signaling ensures partial progression through the preGSB phase, and total progression through GSB and post-GSB phases, it is not required for sclerotization and tanning. These results demonstrate a new transient epidermis to neuron signaling event that facilitates progression through the cascade of pupariationassociated behaviors.Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; PortugalFil: Heredia, Fabiana. Universidade Nova de Lisboa; PortugalFil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; PortugalFil: Pereirinha, Joana. Universidade Nova de Lisboa; PortugalFil: Casimiro, Andrea. Universidade Nova de Lisboa; PortugalFil: Viegas, Filipe. Universidade Nova de Lisboa; PortugalFil: Belém, Claudia. Universidade Nova de Lisboa; PortugalFil: Tanaka, Kohtaro. Instituto Gulbenkian de Ciências; PortugalFil: Cardoso, Gisela. Universidade Nova de Lisboa; Portugal. Universidade de Sao Paulo; BrasilFil: Macedo, Andre. Universidade Nova de Lisboa; PortugalFil: Leal, Ana. Universidade Nova de Lisboa; PortugalFil: Kotowicz, Malwina. Universidade Nova de Lisboa; PortugalFil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Mendes, César. Universidade Nova de Lisboa; PortugalFil: Gontijo, Alisson M.. Universidade Nova de Lisboa; PortugalInsect Hormones 2019KolymbariGreciaBashirullah, ArashLeopold, PierreInsect Hormones2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194589Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons; Insect Hormones 2019; Kolymbari; Grecia; 2019; 67-67CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://insecthormones.org/crete-2019/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:30:59Zoai:ri.conicet.gov.ar:11336/194589instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:31:00.061CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons
title Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons
spellingShingle Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons
Garelli, Andres
DROSOPHILA
DILP8
LGR3
title_short Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons
title_full Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons
title_fullStr Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons
title_full_unstemmed Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons
title_sort Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons
dc.creator.none.fl_str_mv Garelli, Andres
Heredia, Fabiana
Volonté, Yanel Andrea
Pereirinha, Joana
Casimiro, Andrea
Viegas, Filipe
Belém, Claudia
Tanaka, Kohtaro
Cardoso, Gisela
Macedo, Andre
Leal, Ana
Kotowicz, Malwina
Prado Spalm, Facundo Heber
Mendes, César
Gontijo, Alisson M.
author Garelli, Andres
author_facet Garelli, Andres
Heredia, Fabiana
Volonté, Yanel Andrea
Pereirinha, Joana
Casimiro, Andrea
Viegas, Filipe
Belém, Claudia
Tanaka, Kohtaro
Cardoso, Gisela
Macedo, Andre
Leal, Ana
Kotowicz, Malwina
Prado Spalm, Facundo Heber
Mendes, César
Gontijo, Alisson M.
author_role author
author2 Heredia, Fabiana
Volonté, Yanel Andrea
Pereirinha, Joana
Casimiro, Andrea
Viegas, Filipe
Belém, Claudia
Tanaka, Kohtaro
Cardoso, Gisela
Macedo, Andre
Leal, Ana
Kotowicz, Malwina
Prado Spalm, Facundo Heber
Mendes, César
Gontijo, Alisson M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DROSOPHILA
DILP8
LGR3
topic DROSOPHILA
DILP8
LGR3
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Higher dipterans undergo metamorphosis within a puparium, a protective capsule made up of the reshaped and hardened cuticle of the last larval instar. Puparium formation (pupariation) in Drosophila starts when wandering stage larvae reduce their locomotion and initiate increasingly strong whole body contractions that together with the internalization of the three anteriormost body segments remodel the body and cuticle, reducing their length/width ratio from ~5 to ~3. This behavior lasts 5-10 min and culminates with the extrusion of a proteinaceous mix (glue) produced by the salivary glands. The animal then slowly moves forward in a caterpillar-like fashion, traveling about half its length for ~1 min to its final pupariation site. We call this behavior glue-spreading behavior (GSB), as it helps to spread the glue over the ventral part of the animal, promoting its attachment to the underlying substrate. Following GSB, the final shape of the puparium is set, even though weak and periodic contractions occur over the next 40-50 min (post-GSB), the operculum becomes defined, and the cuticle (i.e., the future puparium) starts to gradually sclerotize and tan. Here, we show that proper progression through three pupariation behaviors (pre-GSB, GSB, and postGSB) requires the Dilp8-Lgr3 pathway, a relaxin-like pathway that has been previously implicated in controlling the timing of pupariation in animals carrying aberrantly growing imaginal discs. During pupariation, however, Dilp8-Lgr3 signaling is spatiallyand temporally-distinct: a strong, epidermis-derived dilp8 expression peak that starts at the pre-GSB phase of pupariation appears to signal via Lgr3 in a novel subpopulation of thoracic CNS neurons. While this signaling ensures partial progression through the preGSB phase, and total progression through GSB and post-GSB phases, it is not required for sclerotization and tanning. These results demonstrate a new transient epidermis to neuron signaling event that facilitates progression through the cascade of pupariationassociated behaviors.
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; Portugal
Fil: Heredia, Fabiana. Universidade Nova de Lisboa; Portugal
Fil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; Portugal
Fil: Pereirinha, Joana. Universidade Nova de Lisboa; Portugal
Fil: Casimiro, Andrea. Universidade Nova de Lisboa; Portugal
Fil: Viegas, Filipe. Universidade Nova de Lisboa; Portugal
Fil: Belém, Claudia. Universidade Nova de Lisboa; Portugal
Fil: Tanaka, Kohtaro. Instituto Gulbenkian de Ciências; Portugal
Fil: Cardoso, Gisela. Universidade Nova de Lisboa; Portugal. Universidade de Sao Paulo; Brasil
Fil: Macedo, Andre. Universidade Nova de Lisboa; Portugal
Fil: Leal, Ana. Universidade Nova de Lisboa; Portugal
Fil: Kotowicz, Malwina. Universidade Nova de Lisboa; Portugal
Fil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Mendes, César. Universidade Nova de Lisboa; Portugal
Fil: Gontijo, Alisson M.. Universidade Nova de Lisboa; Portugal
Insect Hormones 2019
Kolymbari
Grecia
Bashirullah, Arash
Leopold, Pierre
description Higher dipterans undergo metamorphosis within a puparium, a protective capsule made up of the reshaped and hardened cuticle of the last larval instar. Puparium formation (pupariation) in Drosophila starts when wandering stage larvae reduce their locomotion and initiate increasingly strong whole body contractions that together with the internalization of the three anteriormost body segments remodel the body and cuticle, reducing their length/width ratio from ~5 to ~3. This behavior lasts 5-10 min and culminates with the extrusion of a proteinaceous mix (glue) produced by the salivary glands. The animal then slowly moves forward in a caterpillar-like fashion, traveling about half its length for ~1 min to its final pupariation site. We call this behavior glue-spreading behavior (GSB), as it helps to spread the glue over the ventral part of the animal, promoting its attachment to the underlying substrate. Following GSB, the final shape of the puparium is set, even though weak and periodic contractions occur over the next 40-50 min (post-GSB), the operculum becomes defined, and the cuticle (i.e., the future puparium) starts to gradually sclerotize and tan. Here, we show that proper progression through three pupariation behaviors (pre-GSB, GSB, and postGSB) requires the Dilp8-Lgr3 pathway, a relaxin-like pathway that has been previously implicated in controlling the timing of pupariation in animals carrying aberrantly growing imaginal discs. During pupariation, however, Dilp8-Lgr3 signaling is spatiallyand temporally-distinct: a strong, epidermis-derived dilp8 expression peak that starts at the pre-GSB phase of pupariation appears to signal via Lgr3 in a novel subpopulation of thoracic CNS neurons. While this signaling ensures partial progression through the preGSB phase, and total progression through GSB and post-GSB phases, it is not required for sclerotization and tanning. These results demonstrate a new transient epidermis to neuron signaling event that facilitates progression through the cascade of pupariationassociated behaviors.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194589
Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons; Insect Hormones 2019; Kolymbari; Grecia; 2019; 67-67
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194589
identifier_str_mv Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons; Insect Hormones 2019; Kolymbari; Grecia; 2019; 67-67
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://insecthormones.org/crete-2019/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Insect Hormones
publisher.none.fl_str_mv Insect Hormones
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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