Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons
- Autores
- Garelli, Andres; Heredia, Fabiana; Volonté, Yanel Andrea; Pereirinha, Joana; Casimiro, Andrea; Viegas, Filipe; Belém, Claudia; Tanaka, Kohtaro; Cardoso, Gisela; Macedo, Andre; Leal, Ana; Kotowicz, Malwina; Prado Spalm, Facundo Heber; Mendes, César; Gontijo, Alisson M.
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Higher dipterans undergo metamorphosis within a puparium, a protective capsule made up of the reshaped and hardened cuticle of the last larval instar. Puparium formation (pupariation) in Drosophila starts when wandering stage larvae reduce their locomotion and initiate increasingly strong whole body contractions that together with the internalization of the three anteriormost body segments remodel the body and cuticle, reducing their length/width ratio from ~5 to ~3. This behavior lasts 5-10 min and culminates with the extrusion of a proteinaceous mix (glue) produced by the salivary glands. The animal then slowly moves forward in a caterpillar-like fashion, traveling about half its length for ~1 min to its final pupariation site. We call this behavior glue-spreading behavior (GSB), as it helps to spread the glue over the ventral part of the animal, promoting its attachment to the underlying substrate. Following GSB, the final shape of the puparium is set, even though weak and periodic contractions occur over the next 40-50 min (post-GSB), the operculum becomes defined, and the cuticle (i.e., the future puparium) starts to gradually sclerotize and tan. Here, we show that proper progression through three pupariation behaviors (pre-GSB, GSB, and postGSB) requires the Dilp8-Lgr3 pathway, a relaxin-like pathway that has been previously implicated in controlling the timing of pupariation in animals carrying aberrantly growing imaginal discs. During pupariation, however, Dilp8-Lgr3 signaling is spatiallyand temporally-distinct: a strong, epidermis-derived dilp8 expression peak that starts at the pre-GSB phase of pupariation appears to signal via Lgr3 in a novel subpopulation of thoracic CNS neurons. While this signaling ensures partial progression through the preGSB phase, and total progression through GSB and post-GSB phases, it is not required for sclerotization and tanning. These results demonstrate a new transient epidermis to neuron signaling event that facilitates progression through the cascade of pupariationassociated behaviors.
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; Portugal
Fil: Heredia, Fabiana. Universidade Nova de Lisboa; Portugal
Fil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; Portugal
Fil: Pereirinha, Joana. Universidade Nova de Lisboa; Portugal
Fil: Casimiro, Andrea. Universidade Nova de Lisboa; Portugal
Fil: Viegas, Filipe. Universidade Nova de Lisboa; Portugal
Fil: Belém, Claudia. Universidade Nova de Lisboa; Portugal
Fil: Tanaka, Kohtaro. Instituto Gulbenkian de Ciências; Portugal
Fil: Cardoso, Gisela. Universidade Nova de Lisboa; Portugal. Universidade de Sao Paulo; Brasil
Fil: Macedo, Andre. Universidade Nova de Lisboa; Portugal
Fil: Leal, Ana. Universidade Nova de Lisboa; Portugal
Fil: Kotowicz, Malwina. Universidade Nova de Lisboa; Portugal
Fil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Mendes, César. Universidade Nova de Lisboa; Portugal
Fil: Gontijo, Alisson M.. Universidade Nova de Lisboa; Portugal
Insect Hormones 2019
Kolymbari
Grecia
Bashirullah, Arash
Leopold, Pierre - Materia
-
DROSOPHILA
DILP8
LGR3 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/194589
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Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neuronsGarelli, AndresHeredia, FabianaVolonté, Yanel AndreaPereirinha, JoanaCasimiro, AndreaViegas, FilipeBelém, ClaudiaTanaka, KohtaroCardoso, GiselaMacedo, AndreLeal, AnaKotowicz, MalwinaPrado Spalm, Facundo HeberMendes, CésarGontijo, Alisson M.DROSOPHILADILP8LGR3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Higher dipterans undergo metamorphosis within a puparium, a protective capsule made up of the reshaped and hardened cuticle of the last larval instar. Puparium formation (pupariation) in Drosophila starts when wandering stage larvae reduce their locomotion and initiate increasingly strong whole body contractions that together with the internalization of the three anteriormost body segments remodel the body and cuticle, reducing their length/width ratio from ~5 to ~3. This behavior lasts 5-10 min and culminates with the extrusion of a proteinaceous mix (glue) produced by the salivary glands. The animal then slowly moves forward in a caterpillar-like fashion, traveling about half its length for ~1 min to its final pupariation site. We call this behavior glue-spreading behavior (GSB), as it helps to spread the glue over the ventral part of the animal, promoting its attachment to the underlying substrate. Following GSB, the final shape of the puparium is set, even though weak and periodic contractions occur over the next 40-50 min (post-GSB), the operculum becomes defined, and the cuticle (i.e., the future puparium) starts to gradually sclerotize and tan. Here, we show that proper progression through three pupariation behaviors (pre-GSB, GSB, and postGSB) requires the Dilp8-Lgr3 pathway, a relaxin-like pathway that has been previously implicated in controlling the timing of pupariation in animals carrying aberrantly growing imaginal discs. During pupariation, however, Dilp8-Lgr3 signaling is spatiallyand temporally-distinct: a strong, epidermis-derived dilp8 expression peak that starts at the pre-GSB phase of pupariation appears to signal via Lgr3 in a novel subpopulation of thoracic CNS neurons. While this signaling ensures partial progression through the preGSB phase, and total progression through GSB and post-GSB phases, it is not required for sclerotization and tanning. These results demonstrate a new transient epidermis to neuron signaling event that facilitates progression through the cascade of pupariationassociated behaviors.Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; PortugalFil: Heredia, Fabiana. Universidade Nova de Lisboa; PortugalFil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; PortugalFil: Pereirinha, Joana. Universidade Nova de Lisboa; PortugalFil: Casimiro, Andrea. Universidade Nova de Lisboa; PortugalFil: Viegas, Filipe. Universidade Nova de Lisboa; PortugalFil: Belém, Claudia. Universidade Nova de Lisboa; PortugalFil: Tanaka, Kohtaro. Instituto Gulbenkian de Ciências; PortugalFil: Cardoso, Gisela. Universidade Nova de Lisboa; Portugal. Universidade de Sao Paulo; BrasilFil: Macedo, Andre. Universidade Nova de Lisboa; PortugalFil: Leal, Ana. Universidade Nova de Lisboa; PortugalFil: Kotowicz, Malwina. Universidade Nova de Lisboa; PortugalFil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Mendes, César. Universidade Nova de Lisboa; PortugalFil: Gontijo, Alisson M.. Universidade Nova de Lisboa; PortugalInsect Hormones 2019KolymbariGreciaBashirullah, ArashLeopold, PierreInsect Hormones2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194589Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons; Insect Hormones 2019; Kolymbari; Grecia; 2019; 67-67CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://insecthormones.org/crete-2019/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:28Zoai:ri.conicet.gov.ar:11336/194589instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:29.013CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons |
title |
Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons |
spellingShingle |
Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons Garelli, Andres DROSOPHILA DILP8 LGR3 |
title_short |
Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons |
title_full |
Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons |
title_fullStr |
Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons |
title_full_unstemmed |
Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons |
title_sort |
Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons |
dc.creator.none.fl_str_mv |
Garelli, Andres Heredia, Fabiana Volonté, Yanel Andrea Pereirinha, Joana Casimiro, Andrea Viegas, Filipe Belém, Claudia Tanaka, Kohtaro Cardoso, Gisela Macedo, Andre Leal, Ana Kotowicz, Malwina Prado Spalm, Facundo Heber Mendes, César Gontijo, Alisson M. |
author |
Garelli, Andres |
author_facet |
Garelli, Andres Heredia, Fabiana Volonté, Yanel Andrea Pereirinha, Joana Casimiro, Andrea Viegas, Filipe Belém, Claudia Tanaka, Kohtaro Cardoso, Gisela Macedo, Andre Leal, Ana Kotowicz, Malwina Prado Spalm, Facundo Heber Mendes, César Gontijo, Alisson M. |
author_role |
author |
author2 |
Heredia, Fabiana Volonté, Yanel Andrea Pereirinha, Joana Casimiro, Andrea Viegas, Filipe Belém, Claudia Tanaka, Kohtaro Cardoso, Gisela Macedo, Andre Leal, Ana Kotowicz, Malwina Prado Spalm, Facundo Heber Mendes, César Gontijo, Alisson M. |
author2_role |
author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
DROSOPHILA DILP8 LGR3 |
topic |
DROSOPHILA DILP8 LGR3 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Higher dipterans undergo metamorphosis within a puparium, a protective capsule made up of the reshaped and hardened cuticle of the last larval instar. Puparium formation (pupariation) in Drosophila starts when wandering stage larvae reduce their locomotion and initiate increasingly strong whole body contractions that together with the internalization of the three anteriormost body segments remodel the body and cuticle, reducing their length/width ratio from ~5 to ~3. This behavior lasts 5-10 min and culminates with the extrusion of a proteinaceous mix (glue) produced by the salivary glands. The animal then slowly moves forward in a caterpillar-like fashion, traveling about half its length for ~1 min to its final pupariation site. We call this behavior glue-spreading behavior (GSB), as it helps to spread the glue over the ventral part of the animal, promoting its attachment to the underlying substrate. Following GSB, the final shape of the puparium is set, even though weak and periodic contractions occur over the next 40-50 min (post-GSB), the operculum becomes defined, and the cuticle (i.e., the future puparium) starts to gradually sclerotize and tan. Here, we show that proper progression through three pupariation behaviors (pre-GSB, GSB, and postGSB) requires the Dilp8-Lgr3 pathway, a relaxin-like pathway that has been previously implicated in controlling the timing of pupariation in animals carrying aberrantly growing imaginal discs. During pupariation, however, Dilp8-Lgr3 signaling is spatiallyand temporally-distinct: a strong, epidermis-derived dilp8 expression peak that starts at the pre-GSB phase of pupariation appears to signal via Lgr3 in a novel subpopulation of thoracic CNS neurons. While this signaling ensures partial progression through the preGSB phase, and total progression through GSB and post-GSB phases, it is not required for sclerotization and tanning. These results demonstrate a new transient epidermis to neuron signaling event that facilitates progression through the cascade of pupariationassociated behaviors. Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; Portugal Fil: Heredia, Fabiana. Universidade Nova de Lisboa; Portugal Fil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; Portugal Fil: Pereirinha, Joana. Universidade Nova de Lisboa; Portugal Fil: Casimiro, Andrea. Universidade Nova de Lisboa; Portugal Fil: Viegas, Filipe. Universidade Nova de Lisboa; Portugal Fil: Belém, Claudia. Universidade Nova de Lisboa; Portugal Fil: Tanaka, Kohtaro. Instituto Gulbenkian de Ciências; Portugal Fil: Cardoso, Gisela. Universidade Nova de Lisboa; Portugal. Universidade de Sao Paulo; Brasil Fil: Macedo, Andre. Universidade Nova de Lisboa; Portugal Fil: Leal, Ana. Universidade Nova de Lisboa; Portugal Fil: Kotowicz, Malwina. Universidade Nova de Lisboa; Portugal Fil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Mendes, César. Universidade Nova de Lisboa; Portugal Fil: Gontijo, Alisson M.. Universidade Nova de Lisboa; Portugal Insect Hormones 2019 Kolymbari Grecia Bashirullah, Arash Leopold, Pierre |
description |
Higher dipterans undergo metamorphosis within a puparium, a protective capsule made up of the reshaped and hardened cuticle of the last larval instar. Puparium formation (pupariation) in Drosophila starts when wandering stage larvae reduce their locomotion and initiate increasingly strong whole body contractions that together with the internalization of the three anteriormost body segments remodel the body and cuticle, reducing their length/width ratio from ~5 to ~3. This behavior lasts 5-10 min and culminates with the extrusion of a proteinaceous mix (glue) produced by the salivary glands. The animal then slowly moves forward in a caterpillar-like fashion, traveling about half its length for ~1 min to its final pupariation site. We call this behavior glue-spreading behavior (GSB), as it helps to spread the glue over the ventral part of the animal, promoting its attachment to the underlying substrate. Following GSB, the final shape of the puparium is set, even though weak and periodic contractions occur over the next 40-50 min (post-GSB), the operculum becomes defined, and the cuticle (i.e., the future puparium) starts to gradually sclerotize and tan. Here, we show that proper progression through three pupariation behaviors (pre-GSB, GSB, and postGSB) requires the Dilp8-Lgr3 pathway, a relaxin-like pathway that has been previously implicated in controlling the timing of pupariation in animals carrying aberrantly growing imaginal discs. During pupariation, however, Dilp8-Lgr3 signaling is spatiallyand temporally-distinct: a strong, epidermis-derived dilp8 expression peak that starts at the pre-GSB phase of pupariation appears to signal via Lgr3 in a novel subpopulation of thoracic CNS neurons. While this signaling ensures partial progression through the preGSB phase, and total progression through GSB and post-GSB phases, it is not required for sclerotization and tanning. These results demonstrate a new transient epidermis to neuron signaling event that facilitates progression through the cascade of pupariationassociated behaviors. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/194589 Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons; Insect Hormones 2019; Kolymbari; Grecia; 2019; 67-67 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/194589 |
identifier_str_mv |
Progression through pupariation behaviors requires dilp8-Lgr3 signaling between the cuticle epidermis and thoracic CNS neurons; Insect Hormones 2019; Kolymbari; Grecia; 2019; 67-67 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://insecthormones.org/crete-2019/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
Insect Hormones |
publisher.none.fl_str_mv |
Insect Hormones |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |