Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
- Autores
- Garelli, Andres; Heredia, Fabiana; Casimiro, Andreia P.; Macedo, Andre; Nunes, Catarina; Garcez, Marcia; Mantas Dias, Angela R.; Volonté, Yanel Andrea; Uhlmann, Thomas; Caparros, Esther; Koyama, Takashi; Gontijo, Alisson M.
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- How different organs in the body sense growth perturbations in distant tissues to coordinatetheir size during development is poorly understood. Here we mutate an invertebrate orphanrelaxin receptor gene, the Drosophila Leucine-rich repeat-containing G protein-coupled receptor 3(Lgr3), and find body asymmetries similar to those found in insulin-like peptide 8 (dilp8)mutants, which fail to coordinate growth with developmental timing. Indeed, mutation or RNAintereference (RNAi) against Lgr3 suppresses the delay in pupariation induced by imaginaldisc growth perturbation or ectopic Dilp8 expression. By tagging endogenous Lgr3 andperforming cell type-specific RNAi, we map this Lgr3 activity to a new subset of CNS neurons,four of which are a pair of bilateral pars intercerebralis Lgr3-positive (PIL) neurons that respondspecifically to ectopic Dilp8 by increasing cAMP-dependent signalling. Our work sheds newlight on the function and evolution of relaxin receptors and reveals a novel neuroendocrinecircuit responsive to growth aberrations.
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina
Fil: Heredia, Fabiana. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Casimiro, Andreia P.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Macedo, Andre. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Nunes, Catarina. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Garcez, Marcia. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Mantas Dias, Angela R.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina
Fil: Uhlmann, Thomas. Dualsystems Biotech Ag; Suiza
Fil: Caparros, Esther. Universidad Miguel Hernández. Facultad de Medicina; España
Fil: Koyama, Takashi. Instituto Gulbenkian de Ciência; Portugal
Fil: Gontijo, Alisson M.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal - Materia
-
DROSPHILA
INSULIN LIKE PEPTIDES
DILP8
LGR3 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/4377
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/4377 |
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Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timingGarelli, AndresHeredia, FabianaCasimiro, Andreia P.Macedo, AndreNunes, CatarinaGarcez, MarciaMantas Dias, Angela R.Volonté, Yanel AndreaUhlmann, ThomasCaparros, EstherKoyama, TakashiGontijo, Alisson M.DROSPHILAINSULIN LIKE PEPTIDESDILP8LGR3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1How different organs in the body sense growth perturbations in distant tissues to coordinatetheir size during development is poorly understood. Here we mutate an invertebrate orphanrelaxin receptor gene, the Drosophila Leucine-rich repeat-containing G protein-coupled receptor 3(Lgr3), and find body asymmetries similar to those found in insulin-like peptide 8 (dilp8)mutants, which fail to coordinate growth with developmental timing. Indeed, mutation or RNAintereference (RNAi) against Lgr3 suppresses the delay in pupariation induced by imaginaldisc growth perturbation or ectopic Dilp8 expression. By tagging endogenous Lgr3 andperforming cell type-specific RNAi, we map this Lgr3 activity to a new subset of CNS neurons,four of which are a pair of bilateral pars intercerebralis Lgr3-positive (PIL) neurons that respondspecifically to ectopic Dilp8 by increasing cAMP-dependent signalling. Our work sheds newlight on the function and evolution of relaxin receptors and reveals a novel neuroendocrinecircuit responsive to growth aberrations.Fil: Garelli, Andres. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); ArgentinaFil: Heredia, Fabiana. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; PortugalFil: Casimiro, Andreia P.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; PortugalFil: Macedo, Andre. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; PortugalFil: Nunes, Catarina. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; PortugalFil: Garcez, Marcia. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; PortugalFil: Mantas Dias, Angela R.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; PortugalFil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); ArgentinaFil: Uhlmann, Thomas. Dualsystems Biotech Ag; SuizaFil: Caparros, Esther. Universidad Miguel Hernández. Facultad de Medicina; EspañaFil: Koyama, Takashi. Instituto Gulbenkian de Ciência; PortugalFil: Gontijo, Alisson M.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4377Garelli, Andres; Heredia, Fabiana; Casimiro, Andreia P.; Macedo, Andre; Nunes, Catarina; et al.; Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing; Nature Communications; 6; 10-2015; 1-142041-1723enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/ncomms/index.htmlinfo:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms9732info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:46Zoai:ri.conicet.gov.ar:11336/4377instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:46.325CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing |
title |
Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing |
spellingShingle |
Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing Garelli, Andres DROSPHILA INSULIN LIKE PEPTIDES DILP8 LGR3 |
title_short |
Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing |
title_full |
Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing |
title_fullStr |
Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing |
title_full_unstemmed |
Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing |
title_sort |
Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing |
dc.creator.none.fl_str_mv |
Garelli, Andres Heredia, Fabiana Casimiro, Andreia P. Macedo, Andre Nunes, Catarina Garcez, Marcia Mantas Dias, Angela R. Volonté, Yanel Andrea Uhlmann, Thomas Caparros, Esther Koyama, Takashi Gontijo, Alisson M. |
author |
Garelli, Andres |
author_facet |
Garelli, Andres Heredia, Fabiana Casimiro, Andreia P. Macedo, Andre Nunes, Catarina Garcez, Marcia Mantas Dias, Angela R. Volonté, Yanel Andrea Uhlmann, Thomas Caparros, Esther Koyama, Takashi Gontijo, Alisson M. |
author_role |
author |
author2 |
Heredia, Fabiana Casimiro, Andreia P. Macedo, Andre Nunes, Catarina Garcez, Marcia Mantas Dias, Angela R. Volonté, Yanel Andrea Uhlmann, Thomas Caparros, Esther Koyama, Takashi Gontijo, Alisson M. |
author2_role |
author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
DROSPHILA INSULIN LIKE PEPTIDES DILP8 LGR3 |
topic |
DROSPHILA INSULIN LIKE PEPTIDES DILP8 LGR3 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
How different organs in the body sense growth perturbations in distant tissues to coordinatetheir size during development is poorly understood. Here we mutate an invertebrate orphanrelaxin receptor gene, the Drosophila Leucine-rich repeat-containing G protein-coupled receptor 3(Lgr3), and find body asymmetries similar to those found in insulin-like peptide 8 (dilp8)mutants, which fail to coordinate growth with developmental timing. Indeed, mutation or RNAintereference (RNAi) against Lgr3 suppresses the delay in pupariation induced by imaginaldisc growth perturbation or ectopic Dilp8 expression. By tagging endogenous Lgr3 andperforming cell type-specific RNAi, we map this Lgr3 activity to a new subset of CNS neurons,four of which are a pair of bilateral pars intercerebralis Lgr3-positive (PIL) neurons that respondspecifically to ectopic Dilp8 by increasing cAMP-dependent signalling. Our work sheds newlight on the function and evolution of relaxin receptors and reveals a novel neuroendocrinecircuit responsive to growth aberrations. Fil: Garelli, Andres. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina Fil: Heredia, Fabiana. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal Fil: Casimiro, Andreia P.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal Fil: Macedo, Andre. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal Fil: Nunes, Catarina. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal Fil: Garcez, Marcia. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal Fil: Mantas Dias, Angela R.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal Fil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina Fil: Uhlmann, Thomas. Dualsystems Biotech Ag; Suiza Fil: Caparros, Esther. Universidad Miguel Hernández. Facultad de Medicina; España Fil: Koyama, Takashi. Instituto Gulbenkian de Ciência; Portugal Fil: Gontijo, Alisson M.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal |
description |
How different organs in the body sense growth perturbations in distant tissues to coordinatetheir size during development is poorly understood. Here we mutate an invertebrate orphanrelaxin receptor gene, the Drosophila Leucine-rich repeat-containing G protein-coupled receptor 3(Lgr3), and find body asymmetries similar to those found in insulin-like peptide 8 (dilp8)mutants, which fail to coordinate growth with developmental timing. Indeed, mutation or RNAintereference (RNAi) against Lgr3 suppresses the delay in pupariation induced by imaginaldisc growth perturbation or ectopic Dilp8 expression. By tagging endogenous Lgr3 andperforming cell type-specific RNAi, we map this Lgr3 activity to a new subset of CNS neurons,four of which are a pair of bilateral pars intercerebralis Lgr3-positive (PIL) neurons that respondspecifically to ectopic Dilp8 by increasing cAMP-dependent signalling. Our work sheds newlight on the function and evolution of relaxin receptors and reveals a novel neuroendocrinecircuit responsive to growth aberrations. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/4377 Garelli, Andres; Heredia, Fabiana; Casimiro, Andreia P.; Macedo, Andre; Nunes, Catarina; et al.; Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing; Nature Communications; 6; 10-2015; 1-14 2041-1723 |
url |
http://hdl.handle.net/11336/4377 |
identifier_str_mv |
Garelli, Andres; Heredia, Fabiana; Casimiro, Andreia P.; Macedo, Andre; Nunes, Catarina; et al.; Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing; Nature Communications; 6; 10-2015; 1-14 2041-1723 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/ info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/ncomms/index.html info:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms9732 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.source.none.fl_str_mv |
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collection |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
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