Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing

Autores
Garelli, Andres; Heredia, Fabiana; Casimiro, Andreia P.; Macedo, Andre; Nunes, Catarina; Garcez, Marcia; Mantas Dias, Angela R.; Volonté, Yanel Andrea; Uhlmann, Thomas; Caparros, Esther; Koyama, Takashi; Gontijo, Alisson M.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
How different organs in the body sense growth perturbations in distant tissues to coordinatetheir size during development is poorly understood. Here we mutate an invertebrate orphanrelaxin receptor gene, the Drosophila Leucine-rich repeat-containing G protein-coupled receptor 3(Lgr3), and find body asymmetries similar to those found in insulin-like peptide 8 (dilp8)mutants, which fail to coordinate growth with developmental timing. Indeed, mutation or RNAintereference (RNAi) against Lgr3 suppresses the delay in pupariation induced by imaginaldisc growth perturbation or ectopic Dilp8 expression. By tagging endogenous Lgr3 andperforming cell type-specific RNAi, we map this Lgr3 activity to a new subset of CNS neurons,four of which are a pair of bilateral pars intercerebralis Lgr3-positive (PIL) neurons that respondspecifically to ectopic Dilp8 by increasing cAMP-dependent signalling. Our work sheds newlight on the function and evolution of relaxin receptors and reveals a novel neuroendocrinecircuit responsive to growth aberrations.
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina
Fil: Heredia, Fabiana. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Casimiro, Andreia P.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Macedo, Andre. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Nunes, Catarina. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Garcez, Marcia. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Mantas Dias, Angela R.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina
Fil: Uhlmann, Thomas. Dualsystems Biotech Ag; Suiza
Fil: Caparros, Esther. Universidad Miguel Hernández. Facultad de Medicina; España
Fil: Koyama, Takashi. Instituto Gulbenkian de Ciência; Portugal
Fil: Gontijo, Alisson M.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Materia
DROSPHILA
INSULIN LIKE PEPTIDES
DILP8
LGR3
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4377
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4377
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timingGarelli, AndresHeredia, FabianaCasimiro, Andreia P.Macedo, AndreNunes, CatarinaGarcez, MarciaMantas Dias, Angela R.Volonté, Yanel AndreaUhlmann, ThomasCaparros, EstherKoyama, TakashiGontijo, Alisson M.DROSPHILAINSULIN LIKE PEPTIDESDILP8LGR3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1How different organs in the body sense growth perturbations in distant tissues to coordinatetheir size during development is poorly understood. Here we mutate an invertebrate orphanrelaxin receptor gene, the Drosophila Leucine-rich repeat-containing G protein-coupled receptor 3(Lgr3), and find body asymmetries similar to those found in insulin-like peptide 8 (dilp8)mutants, which fail to coordinate growth with developmental timing. Indeed, mutation or RNAintereference (RNAi) against Lgr3 suppresses the delay in pupariation induced by imaginaldisc growth perturbation or ectopic Dilp8 expression. By tagging endogenous Lgr3 andperforming cell type-specific RNAi, we map this Lgr3 activity to a new subset of CNS neurons,four of which are a pair of bilateral pars intercerebralis Lgr3-positive (PIL) neurons that respondspecifically to ectopic Dilp8 by increasing cAMP-dependent signalling. Our work sheds newlight on the function and evolution of relaxin receptors and reveals a novel neuroendocrinecircuit responsive to growth aberrations.Fil: Garelli, Andres. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); ArgentinaFil: Heredia, Fabiana. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; PortugalFil: Casimiro, Andreia P.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; PortugalFil: Macedo, Andre. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; PortugalFil: Nunes, Catarina. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; PortugalFil: Garcez, Marcia. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; PortugalFil: Mantas Dias, Angela R.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; PortugalFil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); ArgentinaFil: Uhlmann, Thomas. Dualsystems Biotech Ag; SuizaFil: Caparros, Esther. Universidad Miguel Hernández. Facultad de Medicina; EspañaFil: Koyama, Takashi. Instituto Gulbenkian de Ciência; PortugalFil: Gontijo, Alisson M.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4377Garelli, Andres; Heredia, Fabiana; Casimiro, Andreia P.; Macedo, Andre; Nunes, Catarina; et al.; Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing; Nature Communications; 6; 10-2015; 1-142041-1723enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/ncomms/index.htmlinfo:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms9732info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:07:04Zoai:ri.conicet.gov.ar:11336/4377instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:07:04.565CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
title Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
spellingShingle Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
Garelli, Andres
DROSPHILA
INSULIN LIKE PEPTIDES
DILP8
LGR3
title_short Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
title_full Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
title_fullStr Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
title_full_unstemmed Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
title_sort Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
dc.creator.none.fl_str_mv Garelli, Andres
Heredia, Fabiana
Casimiro, Andreia P.
Macedo, Andre
Nunes, Catarina
Garcez, Marcia
Mantas Dias, Angela R.
Volonté, Yanel Andrea
Uhlmann, Thomas
Caparros, Esther
Koyama, Takashi
Gontijo, Alisson M.
author Garelli, Andres
author_facet Garelli, Andres
Heredia, Fabiana
Casimiro, Andreia P.
Macedo, Andre
Nunes, Catarina
Garcez, Marcia
Mantas Dias, Angela R.
Volonté, Yanel Andrea
Uhlmann, Thomas
Caparros, Esther
Koyama, Takashi
Gontijo, Alisson M.
author_role author
author2 Heredia, Fabiana
Casimiro, Andreia P.
Macedo, Andre
Nunes, Catarina
Garcez, Marcia
Mantas Dias, Angela R.
Volonté, Yanel Andrea
Uhlmann, Thomas
Caparros, Esther
Koyama, Takashi
Gontijo, Alisson M.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DROSPHILA
INSULIN LIKE PEPTIDES
DILP8
LGR3
topic DROSPHILA
INSULIN LIKE PEPTIDES
DILP8
LGR3
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv How different organs in the body sense growth perturbations in distant tissues to coordinatetheir size during development is poorly understood. Here we mutate an invertebrate orphanrelaxin receptor gene, the Drosophila Leucine-rich repeat-containing G protein-coupled receptor 3(Lgr3), and find body asymmetries similar to those found in insulin-like peptide 8 (dilp8)mutants, which fail to coordinate growth with developmental timing. Indeed, mutation or RNAintereference (RNAi) against Lgr3 suppresses the delay in pupariation induced by imaginaldisc growth perturbation or ectopic Dilp8 expression. By tagging endogenous Lgr3 andperforming cell type-specific RNAi, we map this Lgr3 activity to a new subset of CNS neurons,four of which are a pair of bilateral pars intercerebralis Lgr3-positive (PIL) neurons that respondspecifically to ectopic Dilp8 by increasing cAMP-dependent signalling. Our work sheds newlight on the function and evolution of relaxin receptors and reveals a novel neuroendocrinecircuit responsive to growth aberrations.
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina
Fil: Heredia, Fabiana. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Casimiro, Andreia P.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Macedo, Andre. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Nunes, Catarina. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Garcez, Marcia. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Mantas Dias, Angela R.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
Fil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina
Fil: Uhlmann, Thomas. Dualsystems Biotech Ag; Suiza
Fil: Caparros, Esther. Universidad Miguel Hernández. Facultad de Medicina; España
Fil: Koyama, Takashi. Instituto Gulbenkian de Ciência; Portugal
Fil: Gontijo, Alisson M.. Nova University of Lisbon. CEDOC-Chronic Diseases Research Center; Portugal
description How different organs in the body sense growth perturbations in distant tissues to coordinatetheir size during development is poorly understood. Here we mutate an invertebrate orphanrelaxin receptor gene, the Drosophila Leucine-rich repeat-containing G protein-coupled receptor 3(Lgr3), and find body asymmetries similar to those found in insulin-like peptide 8 (dilp8)mutants, which fail to coordinate growth with developmental timing. Indeed, mutation or RNAintereference (RNAi) against Lgr3 suppresses the delay in pupariation induced by imaginaldisc growth perturbation or ectopic Dilp8 expression. By tagging endogenous Lgr3 andperforming cell type-specific RNAi, we map this Lgr3 activity to a new subset of CNS neurons,four of which are a pair of bilateral pars intercerebralis Lgr3-positive (PIL) neurons that respondspecifically to ectopic Dilp8 by increasing cAMP-dependent signalling. Our work sheds newlight on the function and evolution of relaxin receptors and reveals a novel neuroendocrinecircuit responsive to growth aberrations.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4377
Garelli, Andres; Heredia, Fabiana; Casimiro, Andreia P.; Macedo, Andre; Nunes, Catarina; et al.; Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing; Nature Communications; 6; 10-2015; 1-14
2041-1723
url http://hdl.handle.net/11336/4377
identifier_str_mv Garelli, Andres; Heredia, Fabiana; Casimiro, Andreia P.; Macedo, Andre; Nunes, Catarina; et al.; Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing; Nature Communications; 6; 10-2015; 1-14
2041-1723
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/ncomms/index.html
info:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms9732
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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