Epicatechin reduces blood pressure increase in high fructose-fed rats: effects on the determinants of nitric oxide bioavailability

Autores
Litterio, Maria Corina; Vazquez, Marcela Alejandra; Adamo, Ana María; Elesgaray, Rosana; Oteiza, Patricia Isabel; Galleano, Mónica Liliana; Fraga, César Guillermo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work investigated the blood pressure (BP) lowering effect of the flavanol (─)-epicatechin in a model of metabolic syndrome. Rats were fed a regular chow diet without (Control) or with 10% (w/v) fructose in the drinking water (high fructose, HF) for 8 w. A subgroup of the HF-fed rats was supplemented with (─)-epicatechin 20 mg/kg body weight (HF-EC). Dietary (─)-epicatechin reverted the increase in BP caused by the fructose treatment. In aorta, superoxide anion production and the expression of the NADPH oxidase (NOX) subunits p47phox and p22phox were enhanced in the HF-fed rats. The increase was prevented by (─)-epicatechin. Similar profile was observed for NOX4 expression. The activity of aorta nitric oxide synthase (NOS) was increased in the HF group and was even higher in the HF-EC rats. This effect was paralleled by increased endothelial NOS phosphorylation at the activation site Ser1177. Among the more relevant MAPKs pathways in vascular tissue, JNK was shown to be activated in the aorta of the HF-fed rats and (─)-epicatechin supplementation mitigated this effect. Thus, these results suggest that dietary (−)-epicatechin supplementation prevented hypertension in HF-fed rats decreasing superoxide anion production and elevating NOS activity favoring an increase in NO bioavailability.
Fil: Litterio, Maria Corina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Vazquez, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Adamo, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Elesgaray, Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Oteiza, Patricia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California at Davis; Estados Unidos
Fil: Galleano, Mónica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Fraga, César Guillermo. University of California at Davis; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Materia
FLAVONOIDS
HYPERTENSION
METABOLIC SYNDROME
OXIDANTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/110839
Acceder
id CONICETDig_3620665b81cd439f2cbffc044aedefdf
oai_identifier_str oai:ri.conicet.gov.ar:11336/110839
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Epicatechin reduces blood pressure increase in high fructose-fed rats: effects on the determinants of nitric oxide bioavailabilityLitterio, Maria CorinaVazquez, Marcela AlejandraAdamo, Ana MaríaElesgaray, RosanaOteiza, Patricia IsabelGalleano, Mónica LilianaFraga, César GuillermoFLAVONOIDSHYPERTENSIONMETABOLIC SYNDROMEOXIDANTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This work investigated the blood pressure (BP) lowering effect of the flavanol (─)-epicatechin in a model of metabolic syndrome. Rats were fed a regular chow diet without (Control) or with 10% (w/v) fructose in the drinking water (high fructose, HF) for 8 w. A subgroup of the HF-fed rats was supplemented with (─)-epicatechin 20 mg/kg body weight (HF-EC). Dietary (─)-epicatechin reverted the increase in BP caused by the fructose treatment. In aorta, superoxide anion production and the expression of the NADPH oxidase (NOX) subunits p47phox and p22phox were enhanced in the HF-fed rats. The increase was prevented by (─)-epicatechin. Similar profile was observed for NOX4 expression. The activity of aorta nitric oxide synthase (NOS) was increased in the HF group and was even higher in the HF-EC rats. This effect was paralleled by increased endothelial NOS phosphorylation at the activation site Ser1177. Among the more relevant MAPKs pathways in vascular tissue, JNK was shown to be activated in the aorta of the HF-fed rats and (─)-epicatechin supplementation mitigated this effect. Thus, these results suggest that dietary (−)-epicatechin supplementation prevented hypertension in HF-fed rats decreasing superoxide anion production and elevating NOS activity favoring an increase in NO bioavailability.Fil: Litterio, Maria Corina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Vazquez, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Adamo, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Elesgaray, Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Oteiza, Patricia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California at Davis; Estados UnidosFil: Galleano, Mónica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Fraga, César Guillermo. University of California at Davis; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaElsevier Science Inc2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/110839Litterio, Maria Corina; Vazquez, Marcela Alejandra; Adamo, Ana María; Elesgaray, Rosana; Oteiza, Patricia Isabel; et al.; Epicatechin reduces blood pressure increase in high fructose-fed rats: effects on the determinants of nitric oxide bioavailability; Elsevier Science Inc; Journal Of Nutritional Biochemistry; 26; 7-2015; 745-7510955-2863CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.journals.elsevier.com/the-journal-of-nutritional-biochemistry/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jnutbio.2015.02.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:30Zoai:ri.conicet.gov.ar:11336/110839instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:30.632CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Epicatechin reduces blood pressure increase in high fructose-fed rats: effects on the determinants of nitric oxide bioavailability
title Epicatechin reduces blood pressure increase in high fructose-fed rats: effects on the determinants of nitric oxide bioavailability
spellingShingle Epicatechin reduces blood pressure increase in high fructose-fed rats: effects on the determinants of nitric oxide bioavailability
Litterio, Maria Corina
FLAVONOIDS
HYPERTENSION
METABOLIC SYNDROME
OXIDANTS
title_short Epicatechin reduces blood pressure increase in high fructose-fed rats: effects on the determinants of nitric oxide bioavailability
title_full Epicatechin reduces blood pressure increase in high fructose-fed rats: effects on the determinants of nitric oxide bioavailability
title_fullStr Epicatechin reduces blood pressure increase in high fructose-fed rats: effects on the determinants of nitric oxide bioavailability
title_full_unstemmed Epicatechin reduces blood pressure increase in high fructose-fed rats: effects on the determinants of nitric oxide bioavailability
title_sort Epicatechin reduces blood pressure increase in high fructose-fed rats: effects on the determinants of nitric oxide bioavailability
dc.creator.none.fl_str_mv Litterio, Maria Corina
Vazquez, Marcela Alejandra
Adamo, Ana María
Elesgaray, Rosana
Oteiza, Patricia Isabel
Galleano, Mónica Liliana
Fraga, César Guillermo
author Litterio, Maria Corina
author_facet Litterio, Maria Corina
Vazquez, Marcela Alejandra
Adamo, Ana María
Elesgaray, Rosana
Oteiza, Patricia Isabel
Galleano, Mónica Liliana
Fraga, César Guillermo
author_role author
author2 Vazquez, Marcela Alejandra
Adamo, Ana María
Elesgaray, Rosana
Oteiza, Patricia Isabel
Galleano, Mónica Liliana
Fraga, César Guillermo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv FLAVONOIDS
HYPERTENSION
METABOLIC SYNDROME
OXIDANTS
topic FLAVONOIDS
HYPERTENSION
METABOLIC SYNDROME
OXIDANTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work investigated the blood pressure (BP) lowering effect of the flavanol (─)-epicatechin in a model of metabolic syndrome. Rats were fed a regular chow diet without (Control) or with 10% (w/v) fructose in the drinking water (high fructose, HF) for 8 w. A subgroup of the HF-fed rats was supplemented with (─)-epicatechin 20 mg/kg body weight (HF-EC). Dietary (─)-epicatechin reverted the increase in BP caused by the fructose treatment. In aorta, superoxide anion production and the expression of the NADPH oxidase (NOX) subunits p47phox and p22phox were enhanced in the HF-fed rats. The increase was prevented by (─)-epicatechin. Similar profile was observed for NOX4 expression. The activity of aorta nitric oxide synthase (NOS) was increased in the HF group and was even higher in the HF-EC rats. This effect was paralleled by increased endothelial NOS phosphorylation at the activation site Ser1177. Among the more relevant MAPKs pathways in vascular tissue, JNK was shown to be activated in the aorta of the HF-fed rats and (─)-epicatechin supplementation mitigated this effect. Thus, these results suggest that dietary (−)-epicatechin supplementation prevented hypertension in HF-fed rats decreasing superoxide anion production and elevating NOS activity favoring an increase in NO bioavailability.
Fil: Litterio, Maria Corina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Vazquez, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Adamo, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Elesgaray, Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Oteiza, Patricia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California at Davis; Estados Unidos
Fil: Galleano, Mónica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Fraga, César Guillermo. University of California at Davis; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
description This work investigated the blood pressure (BP) lowering effect of the flavanol (─)-epicatechin in a model of metabolic syndrome. Rats were fed a regular chow diet without (Control) or with 10% (w/v) fructose in the drinking water (high fructose, HF) for 8 w. A subgroup of the HF-fed rats was supplemented with (─)-epicatechin 20 mg/kg body weight (HF-EC). Dietary (─)-epicatechin reverted the increase in BP caused by the fructose treatment. In aorta, superoxide anion production and the expression of the NADPH oxidase (NOX) subunits p47phox and p22phox were enhanced in the HF-fed rats. The increase was prevented by (─)-epicatechin. Similar profile was observed for NOX4 expression. The activity of aorta nitric oxide synthase (NOS) was increased in the HF group and was even higher in the HF-EC rats. This effect was paralleled by increased endothelial NOS phosphorylation at the activation site Ser1177. Among the more relevant MAPKs pathways in vascular tissue, JNK was shown to be activated in the aorta of the HF-fed rats and (─)-epicatechin supplementation mitigated this effect. Thus, these results suggest that dietary (−)-epicatechin supplementation prevented hypertension in HF-fed rats decreasing superoxide anion production and elevating NOS activity favoring an increase in NO bioavailability.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/110839
Litterio, Maria Corina; Vazquez, Marcela Alejandra; Adamo, Ana María; Elesgaray, Rosana; Oteiza, Patricia Isabel; et al.; Epicatechin reduces blood pressure increase in high fructose-fed rats: effects on the determinants of nitric oxide bioavailability; Elsevier Science Inc; Journal Of Nutritional Biochemistry; 26; 7-2015; 745-751
0955-2863
CONICET Digital
CONICET
url http://hdl.handle.net/11336/110839
identifier_str_mv Litterio, Maria Corina; Vazquez, Marcela Alejandra; Adamo, Ana María; Elesgaray, Rosana; Oteiza, Patricia Isabel; et al.; Epicatechin reduces blood pressure increase in high fructose-fed rats: effects on the determinants of nitric oxide bioavailability; Elsevier Science Inc; Journal Of Nutritional Biochemistry; 26; 7-2015; 745-751
0955-2863
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.journals.elsevier.com/the-journal-of-nutritional-biochemistry/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jnutbio.2015.02.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269699965976576
score 13.13397

Publicaciones similares