(–)-Epicatechin reduces blood pressure and improves vasorelaxation in spontaneously hypertensive rats by NO-mediated mechanism

Autores
Galleano, Mónica Liliana; Bernatova, Iveta; Puzserova, Angelika; Balis, Peter; Sestakova, Natalia; Pechanova, Olga; Fraga, César Guillermo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Studies in humans have found consumption of certain flavanoid-containing foods to be associated with improvement in endothelial function and with reduction of blood pressure (BP). (-)-Epicatechin is a compound representative of the flavanols (a subfamily of flavonoids), abundant in cocoa seeds, which is preserved during the industrialization process to chocolate. The antihypertensive effect of dietary (-)-epicatechin was investigated on spontaneously hypertensive rats (SHR). Consumption of (-)-epicatechin-supplemented diet (3 g (-)-epicatechin/kg diet) decreased blood pressu re (BP) in SHR by 27 and 23 mm of Hg on days 2 and 6, respectively. On day 6,a 173% increase of nitric oxide (NO) synthase activity was observed in the aorta of EPI-SHR as compared to non-supplemented SHR (p<0.05). Responses to acetylcholine (ACh) were then examined in femoral arteries in the absence and the presence of with L-NAME, a non-selective NOS inhibitor, to assess the Ach-mediated relaxation ascribed to NO-dependent and independent mechanisms Acetylcholine-induced endothelium-dependent relaxation in the femoral artery was significantly higher in EPI-SHR than in SHR, with a predominance of the NO-dependent component of this relaxation. The endothelium-independent relaxation, assayed by using the NO donor sodium nitroprusside (SNP), resulted non significant different in the three experimental groups, demonstrating an unaffected function of vascular smooth muscle cells. These results give further support to the concept that (-)-epicatechin can modulate BP in hypertension by increasing NO levels in the vasculature.
Fil: Galleano, Mónica Liliana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Bernatova, Iveta. Slovak Academy of Sciences; Eslovaquia
Fil: Puzserova, Angelika. Slovak Academy of Sciences; Eslovaquia
Fil: Balis, Peter. Slovak Academy of Sciences; Eslovaquia
Fil: Sestakova, Natalia. Slovak Academy of Sciences; Eslovaquia
Fil: Pechanova, Olga. Slovak Academy of Sciences; Eslovaquia
Fil: Fraga, César Guillermo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Materia
Flavonoids
Hypertension
Nitric Oxide
Phytonutrients
Cocoa
Chocolate
Endothelial Dysfunction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21283
Acceder
id CONICETDig_1f04e1991bd2bcf0d501484239841699
oai_identifier_str oai:ri.conicet.gov.ar:11336/21283
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling (–)-Epicatechin reduces blood pressure and improves vasorelaxation in spontaneously hypertensive rats by NO-mediated mechanismGalleano, Mónica LilianaBernatova, IvetaPuzserova, AngelikaBalis, PeterSestakova, NataliaPechanova, OlgaFraga, César GuillermoFlavonoidsHypertensionNitric OxidePhytonutrientsCocoaChocolateEndothelial Dysfunctionhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Studies in humans have found consumption of certain flavanoid-containing foods to be associated with improvement in endothelial function and with reduction of blood pressure (BP). (-)-Epicatechin is a compound representative of the flavanols (a subfamily of flavonoids), abundant in cocoa seeds, which is preserved during the industrialization process to chocolate. The antihypertensive effect of dietary (-)-epicatechin was investigated on spontaneously hypertensive rats (SHR). Consumption of (-)-epicatechin-supplemented diet (3 g (-)-epicatechin/kg diet) decreased blood pressu re (BP) in SHR by 27 and 23 mm of Hg on days 2 and 6, respectively. On day 6,a 173% increase of nitric oxide (NO) synthase activity was observed in the aorta of EPI-SHR as compared to non-supplemented SHR (p<0.05). Responses to acetylcholine (ACh) were then examined in femoral arteries in the absence and the presence of with L-NAME, a non-selective NOS inhibitor, to assess the Ach-mediated relaxation ascribed to NO-dependent and independent mechanisms Acetylcholine-induced endothelium-dependent relaxation in the femoral artery was significantly higher in EPI-SHR than in SHR, with a predominance of the NO-dependent component of this relaxation. The endothelium-independent relaxation, assayed by using the NO donor sodium nitroprusside (SNP), resulted non significant different in the three experimental groups, demonstrating an unaffected function of vascular smooth muscle cells. These results give further support to the concept that (-)-epicatechin can modulate BP in hypertension by increasing NO levels in the vasculature.Fil: Galleano, Mónica Liliana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Bernatova, Iveta. Slovak Academy of Sciences; EslovaquiaFil: Puzserova, Angelika. Slovak Academy of Sciences; EslovaquiaFil: Balis, Peter. Slovak Academy of Sciences; EslovaquiaFil: Sestakova, Natalia. Slovak Academy of Sciences; EslovaquiaFil: Pechanova, Olga. Slovak Academy of Sciences; EslovaquiaFil: Fraga, César Guillermo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaWiley2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21283Galleano, Mónica Liliana; Bernatova, Iveta; Puzserova, Angelika; Balis, Peter; Sestakova, Natalia; et al.; (–)-Epicatechin reduces blood pressure and improves vasorelaxation in spontaneously hypertensive rats by NO-mediated mechanism; Wiley; IUBMB Life; 65; 8; 8-2013; 710-7151521-65431521-6551CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/iub.1185info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/iub.1185/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:41Zoai:ri.conicet.gov.ar:11336/21283instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:41.416CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv (–)-Epicatechin reduces blood pressure and improves vasorelaxation in spontaneously hypertensive rats by NO-mediated mechanism
title (–)-Epicatechin reduces blood pressure and improves vasorelaxation in spontaneously hypertensive rats by NO-mediated mechanism
spellingShingle (–)-Epicatechin reduces blood pressure and improves vasorelaxation in spontaneously hypertensive rats by NO-mediated mechanism
Galleano, Mónica Liliana
Flavonoids
Hypertension
Nitric Oxide
Phytonutrients
Cocoa
Chocolate
Endothelial Dysfunction
title_short (–)-Epicatechin reduces blood pressure and improves vasorelaxation in spontaneously hypertensive rats by NO-mediated mechanism
title_full (–)-Epicatechin reduces blood pressure and improves vasorelaxation in spontaneously hypertensive rats by NO-mediated mechanism
title_fullStr (–)-Epicatechin reduces blood pressure and improves vasorelaxation in spontaneously hypertensive rats by NO-mediated mechanism
title_full_unstemmed (–)-Epicatechin reduces blood pressure and improves vasorelaxation in spontaneously hypertensive rats by NO-mediated mechanism
title_sort (–)-Epicatechin reduces blood pressure and improves vasorelaxation in spontaneously hypertensive rats by NO-mediated mechanism
dc.creator.none.fl_str_mv Galleano, Mónica Liliana
Bernatova, Iveta
Puzserova, Angelika
Balis, Peter
Sestakova, Natalia
Pechanova, Olga
Fraga, César Guillermo
author Galleano, Mónica Liliana
author_facet Galleano, Mónica Liliana
Bernatova, Iveta
Puzserova, Angelika
Balis, Peter
Sestakova, Natalia
Pechanova, Olga
Fraga, César Guillermo
author_role author
author2 Bernatova, Iveta
Puzserova, Angelika
Balis, Peter
Sestakova, Natalia
Pechanova, Olga
Fraga, César Guillermo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Flavonoids
Hypertension
Nitric Oxide
Phytonutrients
Cocoa
Chocolate
Endothelial Dysfunction
topic Flavonoids
Hypertension
Nitric Oxide
Phytonutrients
Cocoa
Chocolate
Endothelial Dysfunction
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Studies in humans have found consumption of certain flavanoid-containing foods to be associated with improvement in endothelial function and with reduction of blood pressure (BP). (-)-Epicatechin is a compound representative of the flavanols (a subfamily of flavonoids), abundant in cocoa seeds, which is preserved during the industrialization process to chocolate. The antihypertensive effect of dietary (-)-epicatechin was investigated on spontaneously hypertensive rats (SHR). Consumption of (-)-epicatechin-supplemented diet (3 g (-)-epicatechin/kg diet) decreased blood pressu re (BP) in SHR by 27 and 23 mm of Hg on days 2 and 6, respectively. On day 6,a 173% increase of nitric oxide (NO) synthase activity was observed in the aorta of EPI-SHR as compared to non-supplemented SHR (p<0.05). Responses to acetylcholine (ACh) were then examined in femoral arteries in the absence and the presence of with L-NAME, a non-selective NOS inhibitor, to assess the Ach-mediated relaxation ascribed to NO-dependent and independent mechanisms Acetylcholine-induced endothelium-dependent relaxation in the femoral artery was significantly higher in EPI-SHR than in SHR, with a predominance of the NO-dependent component of this relaxation. The endothelium-independent relaxation, assayed by using the NO donor sodium nitroprusside (SNP), resulted non significant different in the three experimental groups, demonstrating an unaffected function of vascular smooth muscle cells. These results give further support to the concept that (-)-epicatechin can modulate BP in hypertension by increasing NO levels in the vasculature.
Fil: Galleano, Mónica Liliana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Bernatova, Iveta. Slovak Academy of Sciences; Eslovaquia
Fil: Puzserova, Angelika. Slovak Academy of Sciences; Eslovaquia
Fil: Balis, Peter. Slovak Academy of Sciences; Eslovaquia
Fil: Sestakova, Natalia. Slovak Academy of Sciences; Eslovaquia
Fil: Pechanova, Olga. Slovak Academy of Sciences; Eslovaquia
Fil: Fraga, César Guillermo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
description Studies in humans have found consumption of certain flavanoid-containing foods to be associated with improvement in endothelial function and with reduction of blood pressure (BP). (-)-Epicatechin is a compound representative of the flavanols (a subfamily of flavonoids), abundant in cocoa seeds, which is preserved during the industrialization process to chocolate. The antihypertensive effect of dietary (-)-epicatechin was investigated on spontaneously hypertensive rats (SHR). Consumption of (-)-epicatechin-supplemented diet (3 g (-)-epicatechin/kg diet) decreased blood pressu re (BP) in SHR by 27 and 23 mm of Hg on days 2 and 6, respectively. On day 6,a 173% increase of nitric oxide (NO) synthase activity was observed in the aorta of EPI-SHR as compared to non-supplemented SHR (p<0.05). Responses to acetylcholine (ACh) were then examined in femoral arteries in the absence and the presence of with L-NAME, a non-selective NOS inhibitor, to assess the Ach-mediated relaxation ascribed to NO-dependent and independent mechanisms Acetylcholine-induced endothelium-dependent relaxation in the femoral artery was significantly higher in EPI-SHR than in SHR, with a predominance of the NO-dependent component of this relaxation. The endothelium-independent relaxation, assayed by using the NO donor sodium nitroprusside (SNP), resulted non significant different in the three experimental groups, demonstrating an unaffected function of vascular smooth muscle cells. These results give further support to the concept that (-)-epicatechin can modulate BP in hypertension by increasing NO levels in the vasculature.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21283
Galleano, Mónica Liliana; Bernatova, Iveta; Puzserova, Angelika; Balis, Peter; Sestakova, Natalia; et al.; (–)-Epicatechin reduces blood pressure and improves vasorelaxation in spontaneously hypertensive rats by NO-mediated mechanism; Wiley; IUBMB Life; 65; 8; 8-2013; 710-715
1521-6543
1521-6551
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21283
identifier_str_mv Galleano, Mónica Liliana; Bernatova, Iveta; Puzserova, Angelika; Balis, Peter; Sestakova, Natalia; et al.; (–)-Epicatechin reduces blood pressure and improves vasorelaxation in spontaneously hypertensive rats by NO-mediated mechanism; Wiley; IUBMB Life; 65; 8; 8-2013; 710-715
1521-6543
1521-6551
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/iub.1185
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/iub.1185/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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