Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products

Autores
Ortiz, Gastón Ezequiel; Guitart, María Eugenia; Cavalitto, Sebastian Fernando; Alberto, Edgardo Omar; Fernandez Lahore, Marcelo; Blasco, Martín
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The application of cellulases in saccharification processes is restricted by its production cost. Consequently, new fungal strains able to elaborate higher cellulases titers and with special activity profiles are required to make the process economical. The aim of this investigation was to find a promising wild-type Trichoderma strain for cellulases production. The Trichoderma reesei strain 938 (CBS 836.91) was selected among twenty strains on the basis of cellulase-agar-plate screening. Evaluation of the selected strain on six solid substrates indicated the highest activities to be obtained from wheat bran. Statistical analyses of the experimental design indicated a significant effect of pH and moisture on the generation of endoglucanase (EGA) and filter-paper (FPA) activity. Furthermore, a central-composite design-based optimization revealed that pH values between 6.4 and 6.6 and moisture from 74 to 94 % were optimal for cellulases production. Under these conditions, 8?10 IU gds-1 of FPA and 15.6?17.8 IU gds-1 of EGA were obtained. In addition, cultivation in a rotating-drum reactor under optimal conditions gave 8.2 IU gds-1 FPA and 13.5 IU gds-1 EGA. Biochemical characterization of T. reesei 938 cellulases indicated a substantially higher resistance to 4 mM Fe?2 and a slightly greater tolerance to alkaline pH in comparison to Celluclast. These results suggest that T. reesei 938 could be a promising candidate for improved cellulases production through direct-evolution strategies.
Fil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universitat Bremen. School Of Enigineerring And Science Jacobs; Alemania
Fil: Guitart, María Eugenia. Universitat Bremen. School Of Enigineerring And Science Jacobs; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Fil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Fernandez Lahore, Marcelo. Universitat Bremen. School Of Enigineerring And Science Jacobs; Alemania
Fil: Blasco, Martín. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo en Biotecnología Industrial; Argentina
Materia
Trichoderma Reesei
Cellulases Production
Solid-State Fermentation
Drum Bioreactor
Design of Experiments
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20937
Acceder
id CONICETDig_34d7b25fbd6f33fef82b1119343d4c37
oai_identifier_str oai:ri.conicet.gov.ar:11336/20937
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial productsOrtiz, Gastón EzequielGuitart, María EugeniaCavalitto, Sebastian FernandoAlberto, Edgardo OmarFernandez Lahore, MarceloBlasco, MartínTrichoderma ReeseiCellulases ProductionSolid-State FermentationDrum BioreactorDesign of Experimentshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The application of cellulases in saccharification processes is restricted by its production cost. Consequently, new fungal strains able to elaborate higher cellulases titers and with special activity profiles are required to make the process economical. The aim of this investigation was to find a promising wild-type Trichoderma strain for cellulases production. The Trichoderma reesei strain 938 (CBS 836.91) was selected among twenty strains on the basis of cellulase-agar-plate screening. Evaluation of the selected strain on six solid substrates indicated the highest activities to be obtained from wheat bran. Statistical analyses of the experimental design indicated a significant effect of pH and moisture on the generation of endoglucanase (EGA) and filter-paper (FPA) activity. Furthermore, a central-composite design-based optimization revealed that pH values between 6.4 and 6.6 and moisture from 74 to 94 % were optimal for cellulases production. Under these conditions, 8?10 IU gds-1 of FPA and 15.6?17.8 IU gds-1 of EGA were obtained. In addition, cultivation in a rotating-drum reactor under optimal conditions gave 8.2 IU gds-1 FPA and 13.5 IU gds-1 EGA. Biochemical characterization of T. reesei 938 cellulases indicated a substantially higher resistance to 4 mM Fe?2 and a slightly greater tolerance to alkaline pH in comparison to Celluclast. These results suggest that T. reesei 938 could be a promising candidate for improved cellulases production through direct-evolution strategies.Fil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universitat Bremen. School Of Enigineerring And Science Jacobs; AlemaniaFil: Guitart, María Eugenia. Universitat Bremen. School Of Enigineerring And Science Jacobs; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; ArgentinaFil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Fernandez Lahore, Marcelo. Universitat Bremen. School Of Enigineerring And Science Jacobs; AlemaniaFil: Blasco, Martín. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo en Biotecnología Industrial; ArgentinaSpringer2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20937Ortiz, Gastón Ezequiel; Guitart, María Eugenia; Cavalitto, Sebastian Fernando; Alberto, Edgardo Omar; Fernandez Lahore, Marcelo; et al.; Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products; Springer; Bioprocess And Biosystems Engineering; 38; 11; 8-2015; 2117-21281615-75911615-7605enginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00449-015-1451-2info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00449-015-1451-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:53:41Zoai:ri.conicet.gov.ar:11336/20937instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:53:41.9CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products
title Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products
spellingShingle Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products
Ortiz, Gastón Ezequiel
Trichoderma Reesei
Cellulases Production
Solid-State Fermentation
Drum Bioreactor
Design of Experiments
title_short Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products
title_full Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products
title_fullStr Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products
title_full_unstemmed Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products
title_sort Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products
dc.creator.none.fl_str_mv Ortiz, Gastón Ezequiel
Guitart, María Eugenia
Cavalitto, Sebastian Fernando
Alberto, Edgardo Omar
Fernandez Lahore, Marcelo
Blasco, Martín
author Ortiz, Gastón Ezequiel
author_facet Ortiz, Gastón Ezequiel
Guitart, María Eugenia
Cavalitto, Sebastian Fernando
Alberto, Edgardo Omar
Fernandez Lahore, Marcelo
Blasco, Martín
author_role author
author2 Guitart, María Eugenia
Cavalitto, Sebastian Fernando
Alberto, Edgardo Omar
Fernandez Lahore, Marcelo
Blasco, Martín
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Trichoderma Reesei
Cellulases Production
Solid-State Fermentation
Drum Bioreactor
Design of Experiments
topic Trichoderma Reesei
Cellulases Production
Solid-State Fermentation
Drum Bioreactor
Design of Experiments
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The application of cellulases in saccharification processes is restricted by its production cost. Consequently, new fungal strains able to elaborate higher cellulases titers and with special activity profiles are required to make the process economical. The aim of this investigation was to find a promising wild-type Trichoderma strain for cellulases production. The Trichoderma reesei strain 938 (CBS 836.91) was selected among twenty strains on the basis of cellulase-agar-plate screening. Evaluation of the selected strain on six solid substrates indicated the highest activities to be obtained from wheat bran. Statistical analyses of the experimental design indicated a significant effect of pH and moisture on the generation of endoglucanase (EGA) and filter-paper (FPA) activity. Furthermore, a central-composite design-based optimization revealed that pH values between 6.4 and 6.6 and moisture from 74 to 94 % were optimal for cellulases production. Under these conditions, 8?10 IU gds-1 of FPA and 15.6?17.8 IU gds-1 of EGA were obtained. In addition, cultivation in a rotating-drum reactor under optimal conditions gave 8.2 IU gds-1 FPA and 13.5 IU gds-1 EGA. Biochemical characterization of T. reesei 938 cellulases indicated a substantially higher resistance to 4 mM Fe?2 and a slightly greater tolerance to alkaline pH in comparison to Celluclast. These results suggest that T. reesei 938 could be a promising candidate for improved cellulases production through direct-evolution strategies.
Fil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universitat Bremen. School Of Enigineerring And Science Jacobs; Alemania
Fil: Guitart, María Eugenia. Universitat Bremen. School Of Enigineerring And Science Jacobs; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Fil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Fernandez Lahore, Marcelo. Universitat Bremen. School Of Enigineerring And Science Jacobs; Alemania
Fil: Blasco, Martín. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo en Biotecnología Industrial; Argentina
description The application of cellulases in saccharification processes is restricted by its production cost. Consequently, new fungal strains able to elaborate higher cellulases titers and with special activity profiles are required to make the process economical. The aim of this investigation was to find a promising wild-type Trichoderma strain for cellulases production. The Trichoderma reesei strain 938 (CBS 836.91) was selected among twenty strains on the basis of cellulase-agar-plate screening. Evaluation of the selected strain on six solid substrates indicated the highest activities to be obtained from wheat bran. Statistical analyses of the experimental design indicated a significant effect of pH and moisture on the generation of endoglucanase (EGA) and filter-paper (FPA) activity. Furthermore, a central-composite design-based optimization revealed that pH values between 6.4 and 6.6 and moisture from 74 to 94 % were optimal for cellulases production. Under these conditions, 8?10 IU gds-1 of FPA and 15.6?17.8 IU gds-1 of EGA were obtained. In addition, cultivation in a rotating-drum reactor under optimal conditions gave 8.2 IU gds-1 FPA and 13.5 IU gds-1 EGA. Biochemical characterization of T. reesei 938 cellulases indicated a substantially higher resistance to 4 mM Fe?2 and a slightly greater tolerance to alkaline pH in comparison to Celluclast. These results suggest that T. reesei 938 could be a promising candidate for improved cellulases production through direct-evolution strategies.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20937
Ortiz, Gastón Ezequiel; Guitart, María Eugenia; Cavalitto, Sebastian Fernando; Alberto, Edgardo Omar; Fernandez Lahore, Marcelo; et al.; Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products; Springer; Bioprocess And Biosystems Engineering; 38; 11; 8-2015; 2117-2128
1615-7591
1615-7605
url http://hdl.handle.net/11336/20937
identifier_str_mv Ortiz, Gastón Ezequiel; Guitart, María Eugenia; Cavalitto, Sebastian Fernando; Alberto, Edgardo Omar; Fernandez Lahore, Marcelo; et al.; Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products; Springer; Bioprocess And Biosystems Engineering; 38; 11; 8-2015; 2117-2128
1615-7591
1615-7605
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00449-015-1451-2
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00449-015-1451-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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