A Combination of Solid Mandels Medium, CMC, and Congo Red Technique for Rapid, Sensitive and Reproducible Screening of Cellulase-Producing Fungi

Autores
Castrillo, María Lorena; Bich, Gustavo Angel; Amerio, Natalia Soledad; Barengo, Marcela Paola; Rodríguez, María Daniela; Zapata, Pedro Dario; Villalba, Laura Lidia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The bioconversion of lignocellulosic biomass into monomeric sugars, by the action of cellulase enzymes, is the main economic problem hindering the profitable use of this abundant source of energy. Trichoderma genus is important biotechnologically due to their ability to produce a wide spectrum of cellulase enzymes and bioactive compounds. There are different techniques to detected qualitative and quantitative cellulase enzymatic secretion of fungal isolates. In this work, we have analyzed the cellulase secretion from four isolates of Trichoderma genus by qualitative and quantitative assays, with the aim to detect a rapid, sensitive and reproducible way for screening cellulase-producing fungi. Two solid medium in three conditions of pH: 3.5, 4.5 and 5.5 and two incubation time were assayed to evaluate the qualitative enzymatic secretion of fungal isolates. The synergic effect of cellulase complex was quantitatively determined by filter paper activity. The correlation between qualitative screening with Congo red technique and quantitative screening withthe DNS reagent method had already been reported, but not reported the composition of the medium to use for it.In this study, we could standardize a solid medium to detect qualitative cellulase activities with Mandels medium as a nitrogen complex at pH 4, CMC as only carbon source and Congo red technique. This method was rapid, sensitive and reproducible way for screening cellulase-producing fungi.
Fil: Castrillo, María Lorena. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Bich, Gustavo Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Amerio, Natalia Soledad. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Barengo, Marcela Paola. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Rodríguez, María Daniela. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Materia
CELLULASES
TRICHODERMA
ENZYME ASSAYS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/137304
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/137304
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Combination of Solid Mandels Medium, CMC, and Congo Red Technique for Rapid, Sensitive and Reproducible Screening of Cellulase-Producing FungiCastrillo, María LorenaBich, Gustavo AngelAmerio, Natalia SoledadBarengo, Marcela PaolaRodríguez, María DanielaZapata, Pedro DarioVillalba, Laura LidiaCELLULASESTRICHODERMAENZYME ASSAYShttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The bioconversion of lignocellulosic biomass into monomeric sugars, by the action of cellulase enzymes, is the main economic problem hindering the profitable use of this abundant source of energy. Trichoderma genus is important biotechnologically due to their ability to produce a wide spectrum of cellulase enzymes and bioactive compounds. There are different techniques to detected qualitative and quantitative cellulase enzymatic secretion of fungal isolates. In this work, we have analyzed the cellulase secretion from four isolates of Trichoderma genus by qualitative and quantitative assays, with the aim to detect a rapid, sensitive and reproducible way for screening cellulase-producing fungi. Two solid medium in three conditions of pH: 3.5, 4.5 and 5.5 and two incubation time were assayed to evaluate the qualitative enzymatic secretion of fungal isolates. The synergic effect of cellulase complex was quantitatively determined by filter paper activity. The correlation between qualitative screening with Congo red technique and quantitative screening withthe DNS reagent method had already been reported, but not reported the composition of the medium to use for it.In this study, we could standardize a solid medium to detect qualitative cellulase activities with Mandels medium as a nitrogen complex at pH 4, CMC as only carbon source and Congo red technique. This method was rapid, sensitive and reproducible way for screening cellulase-producing fungi.Fil: Castrillo, María Lorena. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Bich, Gustavo Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Amerio, Natalia Soledad. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Barengo, Marcela Paola. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Rodríguez, María Daniela. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaBiogeneric Publishers2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/137304Castrillo, María Lorena; Bich, Gustavo Angel; Amerio, Natalia Soledad; Barengo, Marcela Paola; Rodríguez, María Daniela; et al.; A Combination of Solid Mandels Medium, CMC, and Congo Red Technique for Rapid, Sensitive and Reproducible Screening of Cellulase-Producing Fungi; Biogeneric Publishers; Jorunal of Biogeneric Science and Research; 4; 1; 11-2020; 1-62692-1081CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.46718/JBGSR.2020.05.000130info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:39Zoai:ri.conicet.gov.ar:11336/137304instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:39.776CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Combination of Solid Mandels Medium, CMC, and Congo Red Technique for Rapid, Sensitive and Reproducible Screening of Cellulase-Producing Fungi
title A Combination of Solid Mandels Medium, CMC, and Congo Red Technique for Rapid, Sensitive and Reproducible Screening of Cellulase-Producing Fungi
spellingShingle A Combination of Solid Mandels Medium, CMC, and Congo Red Technique for Rapid, Sensitive and Reproducible Screening of Cellulase-Producing Fungi
Castrillo, María Lorena
CELLULASES
TRICHODERMA
ENZYME ASSAYS
title_short A Combination of Solid Mandels Medium, CMC, and Congo Red Technique for Rapid, Sensitive and Reproducible Screening of Cellulase-Producing Fungi
title_full A Combination of Solid Mandels Medium, CMC, and Congo Red Technique for Rapid, Sensitive and Reproducible Screening of Cellulase-Producing Fungi
title_fullStr A Combination of Solid Mandels Medium, CMC, and Congo Red Technique for Rapid, Sensitive and Reproducible Screening of Cellulase-Producing Fungi
title_full_unstemmed A Combination of Solid Mandels Medium, CMC, and Congo Red Technique for Rapid, Sensitive and Reproducible Screening of Cellulase-Producing Fungi
title_sort A Combination of Solid Mandels Medium, CMC, and Congo Red Technique for Rapid, Sensitive and Reproducible Screening of Cellulase-Producing Fungi
dc.creator.none.fl_str_mv Castrillo, María Lorena
Bich, Gustavo Angel
Amerio, Natalia Soledad
Barengo, Marcela Paola
Rodríguez, María Daniela
Zapata, Pedro Dario
Villalba, Laura Lidia
author Castrillo, María Lorena
author_facet Castrillo, María Lorena
Bich, Gustavo Angel
Amerio, Natalia Soledad
Barengo, Marcela Paola
Rodríguez, María Daniela
Zapata, Pedro Dario
Villalba, Laura Lidia
author_role author
author2 Bich, Gustavo Angel
Amerio, Natalia Soledad
Barengo, Marcela Paola
Rodríguez, María Daniela
Zapata, Pedro Dario
Villalba, Laura Lidia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CELLULASES
TRICHODERMA
ENZYME ASSAYS
topic CELLULASES
TRICHODERMA
ENZYME ASSAYS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The bioconversion of lignocellulosic biomass into monomeric sugars, by the action of cellulase enzymes, is the main economic problem hindering the profitable use of this abundant source of energy. Trichoderma genus is important biotechnologically due to their ability to produce a wide spectrum of cellulase enzymes and bioactive compounds. There are different techniques to detected qualitative and quantitative cellulase enzymatic secretion of fungal isolates. In this work, we have analyzed the cellulase secretion from four isolates of Trichoderma genus by qualitative and quantitative assays, with the aim to detect a rapid, sensitive and reproducible way for screening cellulase-producing fungi. Two solid medium in three conditions of pH: 3.5, 4.5 and 5.5 and two incubation time were assayed to evaluate the qualitative enzymatic secretion of fungal isolates. The synergic effect of cellulase complex was quantitatively determined by filter paper activity. The correlation between qualitative screening with Congo red technique and quantitative screening withthe DNS reagent method had already been reported, but not reported the composition of the medium to use for it.In this study, we could standardize a solid medium to detect qualitative cellulase activities with Mandels medium as a nitrogen complex at pH 4, CMC as only carbon source and Congo red technique. This method was rapid, sensitive and reproducible way for screening cellulase-producing fungi.
Fil: Castrillo, María Lorena. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Bich, Gustavo Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Amerio, Natalia Soledad. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Barengo, Marcela Paola. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Rodríguez, María Daniela. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
description The bioconversion of lignocellulosic biomass into monomeric sugars, by the action of cellulase enzymes, is the main economic problem hindering the profitable use of this abundant source of energy. Trichoderma genus is important biotechnologically due to their ability to produce a wide spectrum of cellulase enzymes and bioactive compounds. There are different techniques to detected qualitative and quantitative cellulase enzymatic secretion of fungal isolates. In this work, we have analyzed the cellulase secretion from four isolates of Trichoderma genus by qualitative and quantitative assays, with the aim to detect a rapid, sensitive and reproducible way for screening cellulase-producing fungi. Two solid medium in three conditions of pH: 3.5, 4.5 and 5.5 and two incubation time were assayed to evaluate the qualitative enzymatic secretion of fungal isolates. The synergic effect of cellulase complex was quantitatively determined by filter paper activity. The correlation between qualitative screening with Congo red technique and quantitative screening withthe DNS reagent method had already been reported, but not reported the composition of the medium to use for it.In this study, we could standardize a solid medium to detect qualitative cellulase activities with Mandels medium as a nitrogen complex at pH 4, CMC as only carbon source and Congo red technique. This method was rapid, sensitive and reproducible way for screening cellulase-producing fungi.
publishDate 2020
dc.date.none.fl_str_mv 2020-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/137304
Castrillo, María Lorena; Bich, Gustavo Angel; Amerio, Natalia Soledad; Barengo, Marcela Paola; Rodríguez, María Daniela; et al.; A Combination of Solid Mandels Medium, CMC, and Congo Red Technique for Rapid, Sensitive and Reproducible Screening of Cellulase-Producing Fungi; Biogeneric Publishers; Jorunal of Biogeneric Science and Research; 4; 1; 11-2020; 1-6
2692-1081
CONICET Digital
CONICET
url http://hdl.handle.net/11336/137304
identifier_str_mv Castrillo, María Lorena; Bich, Gustavo Angel; Amerio, Natalia Soledad; Barengo, Marcela Paola; Rodríguez, María Daniela; et al.; A Combination of Solid Mandels Medium, CMC, and Congo Red Technique for Rapid, Sensitive and Reproducible Screening of Cellulase-Producing Fungi; Biogeneric Publishers; Jorunal of Biogeneric Science and Research; 4; 1; 11-2020; 1-6
2692-1081
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.46718/JBGSR.2020.05.000130
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Biogeneric Publishers
publisher.none.fl_str_mv Biogeneric Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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