Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30

Autores
Ortiz, Gastón Ezequiel; Alberto, Edgardo Omar; Blasco, Martín
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In order to expand the collection of promoters studied in Trichoderma reesei Rut-C30, a functional study of the ToxA promoter was carried out by analysis of GFP expression in T. reesei Rut-C30. For this purpose, the binary pCBCT expression vector was employed to transform T. reesei Rut-C30. The transformants obtained were evaluated by means of fluorescence microscopy, fluorometry, dot blot and Western blot analysis. The low levels of cytoplasmic GFP protein in fungal hyphae suggest that ToxA promoter works as a weak constitutive promoter which can drive successfully the expression of heterologous proteins in T. reesei Rut-C30
Fil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Blasco, Martín. Instituto Nacional de Tecnología Industrial; Argentina
Materia
Trichoderma reesei
ToxA promoter
Recombinant protein expres-sion
Weak promoter.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54189
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30Ortiz, Gastón EzequielAlberto, Edgardo OmarBlasco, MartínTrichoderma reeseiToxA promoterRecombinant protein expres-sionWeak promoter.https://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2In order to expand the collection of promoters studied in Trichoderma reesei Rut-C30, a functional study of the ToxA promoter was carried out by analysis of GFP expression in T. reesei Rut-C30. For this purpose, the binary pCBCT expression vector was employed to transform T. reesei Rut-C30. The transformants obtained were evaluated by means of fluorescence microscopy, fluorometry, dot blot and Western blot analysis. The low levels of cytoplasmic GFP protein in fungal hyphae suggest that ToxA promoter works as a weak constitutive promoter which can drive successfully the expression of heterologous proteins in T. reesei Rut-C30Fil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Blasco, Martín. Instituto Nacional de Tecnología Industrial; ArgentinaTrade science inc.2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54189Ortiz, Gastón Ezequiel; Alberto, Edgardo Omar; Blasco, Martín; Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30; Trade science inc.; BioTechnology: An Indian Journal; 12; 1; 1-2016; 39-440974-7435CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.tsijournals.com/abstract/functional-evaluation-of-toxa-promoter-in-trichoderma-reeseirutc30-2219.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:57:50Zoai:ri.conicet.gov.ar:11336/54189instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:57:50.627CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30
title Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30
spellingShingle Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30
Ortiz, Gastón Ezequiel
Trichoderma reesei
ToxA promoter
Recombinant protein expres-sion
Weak promoter.
title_short Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30
title_full Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30
title_fullStr Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30
title_full_unstemmed Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30
title_sort Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30
dc.creator.none.fl_str_mv Ortiz, Gastón Ezequiel
Alberto, Edgardo Omar
Blasco, Martín
author Ortiz, Gastón Ezequiel
author_facet Ortiz, Gastón Ezequiel
Alberto, Edgardo Omar
Blasco, Martín
author_role author
author2 Alberto, Edgardo Omar
Blasco, Martín
author2_role author
author
dc.subject.none.fl_str_mv Trichoderma reesei
ToxA promoter
Recombinant protein expres-sion
Weak promoter.
topic Trichoderma reesei
ToxA promoter
Recombinant protein expres-sion
Weak promoter.
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In order to expand the collection of promoters studied in Trichoderma reesei Rut-C30, a functional study of the ToxA promoter was carried out by analysis of GFP expression in T. reesei Rut-C30. For this purpose, the binary pCBCT expression vector was employed to transform T. reesei Rut-C30. The transformants obtained were evaluated by means of fluorescence microscopy, fluorometry, dot blot and Western blot analysis. The low levels of cytoplasmic GFP protein in fungal hyphae suggest that ToxA promoter works as a weak constitutive promoter which can drive successfully the expression of heterologous proteins in T. reesei Rut-C30
Fil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Blasco, Martín. Instituto Nacional de Tecnología Industrial; Argentina
description In order to expand the collection of promoters studied in Trichoderma reesei Rut-C30, a functional study of the ToxA promoter was carried out by analysis of GFP expression in T. reesei Rut-C30. For this purpose, the binary pCBCT expression vector was employed to transform T. reesei Rut-C30. The transformants obtained were evaluated by means of fluorescence microscopy, fluorometry, dot blot and Western blot analysis. The low levels of cytoplasmic GFP protein in fungal hyphae suggest that ToxA promoter works as a weak constitutive promoter which can drive successfully the expression of heterologous proteins in T. reesei Rut-C30
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54189
Ortiz, Gastón Ezequiel; Alberto, Edgardo Omar; Blasco, Martín; Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30; Trade science inc.; BioTechnology: An Indian Journal; 12; 1; 1-2016; 39-44
0974-7435
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54189
identifier_str_mv Ortiz, Gastón Ezequiel; Alberto, Edgardo Omar; Blasco, Martín; Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30; Trade science inc.; BioTechnology: An Indian Journal; 12; 1; 1-2016; 39-44
0974-7435
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tsijournals.com/abstract/functional-evaluation-of-toxa-promoter-in-trichoderma-reeseirutc30-2219.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Trade science inc.
publisher.none.fl_str_mv Trade science inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.976206

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