Production of Kluyveromyces spp. and environmental tolerance induction against Aspergillus flavus

Autores
Montemarani, Analía Mariana; Nesci, Andrea Verónica; Etcheverry, Miriam Graciela
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The viability and biomass production of three isolates of Kluyveromyces spp. in six different growth media were studied. All yeast isolates showed good growth in all of the media tested, but nutrient yeast dextrose broth (NYDB 75 %) and molasses soy meal (MSB) media were selected for further analyses. The adaptive response of the yeasts to heat shock and water stress was studied, revealing that 60 min of incubation at 45 °C and a water activity value of 0.95 aw were the appropriate conditions to adapt these yeasts for subsequent analyses. The physiological adaptation did not affect the ecological similarity between biocontrol agents and pathogen. The adapted yeasts also had a negative influence on the growth of Aspergillus flavus RCM89 mycelia and the accumulation of aflatoxin B1 levels in vitro. These results have important implications for optimizing the formulation process of proven biocontrol agents against A. flavus. In addition, the applications of physiological methods are necessary for increasing the performance of biocontrol agents. Moreover, the physiological methods could enhance survival under environmental stress conditions of biological control agents.
Fil: Montemarani, Analía Mariana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nesci, Andrea Verónica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Kluyveromyces
Aspergillus Flavus
Heat Shock
Osmoadaptation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24160
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Production of Kluyveromyces spp. and environmental tolerance induction against Aspergillus flavusMontemarani, Analía MarianaNesci, Andrea VerónicaEtcheverry, Miriam GracielaKluyveromycesAspergillus FlavusHeat ShockOsmoadaptationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The viability and biomass production of three isolates of Kluyveromyces spp. in six different growth media were studied. All yeast isolates showed good growth in all of the media tested, but nutrient yeast dextrose broth (NYDB 75 %) and molasses soy meal (MSB) media were selected for further analyses. The adaptive response of the yeasts to heat shock and water stress was studied, revealing that 60 min of incubation at 45 °C and a water activity value of 0.95 aw were the appropriate conditions to adapt these yeasts for subsequent analyses. The physiological adaptation did not affect the ecological similarity between biocontrol agents and pathogen. The adapted yeasts also had a negative influence on the growth of Aspergillus flavus RCM89 mycelia and the accumulation of aflatoxin B1 levels in vitro. These results have important implications for optimizing the formulation process of proven biocontrol agents against A. flavus. In addition, the applications of physiological methods are necessary for increasing the performance of biocontrol agents. Moreover, the physiological methods could enhance survival under environmental stress conditions of biological control agents.Fil: Montemarani, Analía Mariana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nesci, Andrea Verónica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaInst Microbiologia2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24160Montemarani, Analía Mariana; Nesci, Andrea Verónica; Etcheverry, Miriam Graciela; Production of Kluyveromyces spp. and environmental tolerance induction against Aspergillus flavus; Inst Microbiologia; Annals Of Microbiology; 64; 3; 10-2013; 935-9441590-42611869-2044CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s13213-013-0726-6info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs13213-013-0726-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:29Zoai:ri.conicet.gov.ar:11336/24160instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:29.773CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Production of Kluyveromyces spp. and environmental tolerance induction against Aspergillus flavus
title Production of Kluyveromyces spp. and environmental tolerance induction against Aspergillus flavus
spellingShingle Production of Kluyveromyces spp. and environmental tolerance induction against Aspergillus flavus
Montemarani, Analía Mariana
Kluyveromyces
Aspergillus Flavus
Heat Shock
Osmoadaptation
title_short Production of Kluyveromyces spp. and environmental tolerance induction against Aspergillus flavus
title_full Production of Kluyveromyces spp. and environmental tolerance induction against Aspergillus flavus
title_fullStr Production of Kluyveromyces spp. and environmental tolerance induction against Aspergillus flavus
title_full_unstemmed Production of Kluyveromyces spp. and environmental tolerance induction against Aspergillus flavus
title_sort Production of Kluyveromyces spp. and environmental tolerance induction against Aspergillus flavus
dc.creator.none.fl_str_mv Montemarani, Analía Mariana
Nesci, Andrea Verónica
Etcheverry, Miriam Graciela
author Montemarani, Analía Mariana
author_facet Montemarani, Analía Mariana
Nesci, Andrea Verónica
Etcheverry, Miriam Graciela
author_role author
author2 Nesci, Andrea Verónica
Etcheverry, Miriam Graciela
author2_role author
author
dc.subject.none.fl_str_mv Kluyveromyces
Aspergillus Flavus
Heat Shock
Osmoadaptation
topic Kluyveromyces
Aspergillus Flavus
Heat Shock
Osmoadaptation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The viability and biomass production of three isolates of Kluyveromyces spp. in six different growth media were studied. All yeast isolates showed good growth in all of the media tested, but nutrient yeast dextrose broth (NYDB 75 %) and molasses soy meal (MSB) media were selected for further analyses. The adaptive response of the yeasts to heat shock and water stress was studied, revealing that 60 min of incubation at 45 °C and a water activity value of 0.95 aw were the appropriate conditions to adapt these yeasts for subsequent analyses. The physiological adaptation did not affect the ecological similarity between biocontrol agents and pathogen. The adapted yeasts also had a negative influence on the growth of Aspergillus flavus RCM89 mycelia and the accumulation of aflatoxin B1 levels in vitro. These results have important implications for optimizing the formulation process of proven biocontrol agents against A. flavus. In addition, the applications of physiological methods are necessary for increasing the performance of biocontrol agents. Moreover, the physiological methods could enhance survival under environmental stress conditions of biological control agents.
Fil: Montemarani, Analía Mariana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nesci, Andrea Verónica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The viability and biomass production of three isolates of Kluyveromyces spp. in six different growth media were studied. All yeast isolates showed good growth in all of the media tested, but nutrient yeast dextrose broth (NYDB 75 %) and molasses soy meal (MSB) media were selected for further analyses. The adaptive response of the yeasts to heat shock and water stress was studied, revealing that 60 min of incubation at 45 °C and a water activity value of 0.95 aw were the appropriate conditions to adapt these yeasts for subsequent analyses. The physiological adaptation did not affect the ecological similarity between biocontrol agents and pathogen. The adapted yeasts also had a negative influence on the growth of Aspergillus flavus RCM89 mycelia and the accumulation of aflatoxin B1 levels in vitro. These results have important implications for optimizing the formulation process of proven biocontrol agents against A. flavus. In addition, the applications of physiological methods are necessary for increasing the performance of biocontrol agents. Moreover, the physiological methods could enhance survival under environmental stress conditions of biological control agents.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24160
Montemarani, Analía Mariana; Nesci, Andrea Verónica; Etcheverry, Miriam Graciela; Production of Kluyveromyces spp. and environmental tolerance induction against Aspergillus flavus; Inst Microbiologia; Annals Of Microbiology; 64; 3; 10-2013; 935-944
1590-4261
1869-2044
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24160
identifier_str_mv Montemarani, Analía Mariana; Nesci, Andrea Verónica; Etcheverry, Miriam Graciela; Production of Kluyveromyces spp. and environmental tolerance induction against Aspergillus flavus; Inst Microbiologia; Annals Of Microbiology; 64; 3; 10-2013; 935-944
1590-4261
1869-2044
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s13213-013-0726-6
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs13213-013-0726-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Inst Microbiologia
publisher.none.fl_str_mv Inst Microbiologia
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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