Effects of experimental intracerebroventricular injection of Amylois Beta Peptide (1-42) on tha daily Bax and Bcl-2 expression en the rat hippocampus

Autores
Alfaro, Mauro; Coria, Lucero; Ledesma, Carina Mabel; Castro, Andrea; Mendoza, Gisela Veronica; Anzulovich Miranda, Ana Cecilia; Navigatore Fonzo, Lorena Silvina
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Alzheimer’s disease (AD) is a neurodegenerative disorder marked by cognitive and behavioral impairment. The accumulations of amyloid-β (Aβ) peptides in the brain are believed to be associated with perturbations of synaptic function leading to cognitive deficits. The proto-oncogene Bax (Bcl-2-associated X protein) and related protein Bcl-2 (B-cell chronic lymphocytic leukemia/lymphoma-2) genes are triggers of apoptosis in Alzheimer’s disease (AD). Besides the cognitive deficit, AD patients also show alterations in their circadian rhythms. The objective of this study was to investigate the effects of an intracerebroventricular (i.c.v.) injection of amyloid beta peptide (1-42) on daily patterns of Bax and Bcl-2 expression, as well as of clock proteins in the hippocampus and on temporal profiles of cognitive performance of four-month-old males Holtzman rats. Groups were defined as: control (CO) and Aβ-injected (Aβ). Rats were maintained under 12 h-light:12 h-dark conditions and received food and water ad libitum throughout the entire experimental. Daily rhythms of Bax and Bcl-2 expression were analyzed by RT-PCR and protein levels by Western blots, in hippocampus samples isolated every 4 h during a 24 h period. Regulatory regions of Bax and Bcl-2 were scanned for E-box sites. The Novel Object Recognition (NOR) test was used to evaluate cognition, particularly recognition memory. We found E-box sites on regulatory regions of Bax and Bcl-2 genes, which display a daily oscillation of expression in the rat hippocampus. The i.c.v. injection of Aβ (1-42) modified daily variation of Bax and Bcl-2, and clock proteins. It was observed that the group injected with Aβ explored the novel object for less time compared to the control group, during the day and night periods. Thus, elevated Aβ peptide levels might affect the temporal patterns of cognitive function and apoptotic genes, probably by altering daily rhythms of clock proteins.
Fil: Alfaro, Mauro. Universidad Nacional de San Luis; Argentina
Fil: Coria, Lucero. Universidad Nacional de San Luis; Argentina
Fil: Ledesma, Carina Mabel. Universidad Nacional de San Luis; Argentina
Fil: Castro, Andrea. Universidad Nacional de San Luis; Argentina
Fil: Mendoza, Gisela Veronica. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
XXXIX Reunión Científica Anual de la SBCuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
Materia
INTRACEREBROVENTRICULAR INJECTION OF AMYLOID BETA PEPTIDE
BAX
BCL2
DAILY EXPRESSION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189943
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/189943
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network_name_str CONICET Digital (CONICET)
spelling Effects of experimental intracerebroventricular injection of Amylois Beta Peptide (1-42) on tha daily Bax and Bcl-2 expression en the rat hippocampusAlfaro, MauroCoria, LuceroLedesma, Carina MabelCastro, AndreaMendoza, Gisela VeronicaAnzulovich Miranda, Ana CeciliaNavigatore Fonzo, Lorena SilvinaINTRACEREBROVENTRICULAR INJECTION OF AMYLOID BETA PEPTIDEBAXBCL2DAILY EXPRESSIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Alzheimer’s disease (AD) is a neurodegenerative disorder marked by cognitive and behavioral impairment. The accumulations of amyloid-β (Aβ) peptides in the brain are believed to be associated with perturbations of synaptic function leading to cognitive deficits. The proto-oncogene Bax (Bcl-2-associated X protein) and related protein Bcl-2 (B-cell chronic lymphocytic leukemia/lymphoma-2) genes are triggers of apoptosis in Alzheimer’s disease (AD). Besides the cognitive deficit, AD patients also show alterations in their circadian rhythms. The objective of this study was to investigate the effects of an intracerebroventricular (i.c.v.) injection of amyloid beta peptide (1-42) on daily patterns of Bax and Bcl-2 expression, as well as of clock proteins in the hippocampus and on temporal profiles of cognitive performance of four-month-old males Holtzman rats. Groups were defined as: control (CO) and Aβ-injected (Aβ). Rats were maintained under 12 h-light:12 h-dark conditions and received food and water ad libitum throughout the entire experimental. Daily rhythms of Bax and Bcl-2 expression were analyzed by RT-PCR and protein levels by Western blots, in hippocampus samples isolated every 4 h during a 24 h period. Regulatory regions of Bax and Bcl-2 were scanned for E-box sites. The Novel Object Recognition (NOR) test was used to evaluate cognition, particularly recognition memory. We found E-box sites on regulatory regions of Bax and Bcl-2 genes, which display a daily oscillation of expression in the rat hippocampus. The i.c.v. injection of Aβ (1-42) modified daily variation of Bax and Bcl-2, and clock proteins. It was observed that the group injected with Aβ explored the novel object for less time compared to the control group, during the day and night periods. Thus, elevated Aβ peptide levels might affect the temporal patterns of cognitive function and apoptotic genes, probably by altering daily rhythms of clock proteins.Fil: Alfaro, Mauro. Universidad Nacional de San Luis; ArgentinaFil: Coria, Lucero. Universidad Nacional de San Luis; ArgentinaFil: Ledesma, Carina Mabel. Universidad Nacional de San Luis; ArgentinaFil: Castro, Andrea. Universidad Nacional de San Luis; ArgentinaFil: Mendoza, Gisela Veronica. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaXXXIX Reunión Científica Anual de la SBCuyoSan LuisArgentinaSociedad de Biología de CuyoBiocellCastro Vazquez, Alfredo JuanBarbieri, Manuel Alejandro2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189943Effects of experimental intracerebroventricular injection of Amylois Beta Peptide (1-42) on tha daily Bax and Bcl-2 expression en the rat hippocampus; XXXIX Reunión Científica Anual de la SBCuyo; San Luis; Argentina; 2021; 18-180327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/resumenes-de-la-reunion-2021/info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v46nSuppl.5Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:15Zoai:ri.conicet.gov.ar:11336/189943instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:15.657CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of experimental intracerebroventricular injection of Amylois Beta Peptide (1-42) on tha daily Bax and Bcl-2 expression en the rat hippocampus
title Effects of experimental intracerebroventricular injection of Amylois Beta Peptide (1-42) on tha daily Bax and Bcl-2 expression en the rat hippocampus
spellingShingle Effects of experimental intracerebroventricular injection of Amylois Beta Peptide (1-42) on tha daily Bax and Bcl-2 expression en the rat hippocampus
Alfaro, Mauro
INTRACEREBROVENTRICULAR INJECTION OF AMYLOID BETA PEPTIDE
BAX
BCL2
DAILY EXPRESSION
title_short Effects of experimental intracerebroventricular injection of Amylois Beta Peptide (1-42) on tha daily Bax and Bcl-2 expression en the rat hippocampus
title_full Effects of experimental intracerebroventricular injection of Amylois Beta Peptide (1-42) on tha daily Bax and Bcl-2 expression en the rat hippocampus
title_fullStr Effects of experimental intracerebroventricular injection of Amylois Beta Peptide (1-42) on tha daily Bax and Bcl-2 expression en the rat hippocampus
title_full_unstemmed Effects of experimental intracerebroventricular injection of Amylois Beta Peptide (1-42) on tha daily Bax and Bcl-2 expression en the rat hippocampus
title_sort Effects of experimental intracerebroventricular injection of Amylois Beta Peptide (1-42) on tha daily Bax and Bcl-2 expression en the rat hippocampus
dc.creator.none.fl_str_mv Alfaro, Mauro
Coria, Lucero
Ledesma, Carina Mabel
Castro, Andrea
Mendoza, Gisela Veronica
Anzulovich Miranda, Ana Cecilia
Navigatore Fonzo, Lorena Silvina
author Alfaro, Mauro
author_facet Alfaro, Mauro
Coria, Lucero
Ledesma, Carina Mabel
Castro, Andrea
Mendoza, Gisela Veronica
Anzulovich Miranda, Ana Cecilia
Navigatore Fonzo, Lorena Silvina
author_role author
author2 Coria, Lucero
Ledesma, Carina Mabel
Castro, Andrea
Mendoza, Gisela Veronica
Anzulovich Miranda, Ana Cecilia
Navigatore Fonzo, Lorena Silvina
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Castro Vazquez, Alfredo Juan
Barbieri, Manuel Alejandro
dc.subject.none.fl_str_mv INTRACEREBROVENTRICULAR INJECTION OF AMYLOID BETA PEPTIDE
BAX
BCL2
DAILY EXPRESSION
topic INTRACEREBROVENTRICULAR INJECTION OF AMYLOID BETA PEPTIDE
BAX
BCL2
DAILY EXPRESSION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Alzheimer’s disease (AD) is a neurodegenerative disorder marked by cognitive and behavioral impairment. The accumulations of amyloid-β (Aβ) peptides in the brain are believed to be associated with perturbations of synaptic function leading to cognitive deficits. The proto-oncogene Bax (Bcl-2-associated X protein) and related protein Bcl-2 (B-cell chronic lymphocytic leukemia/lymphoma-2) genes are triggers of apoptosis in Alzheimer’s disease (AD). Besides the cognitive deficit, AD patients also show alterations in their circadian rhythms. The objective of this study was to investigate the effects of an intracerebroventricular (i.c.v.) injection of amyloid beta peptide (1-42) on daily patterns of Bax and Bcl-2 expression, as well as of clock proteins in the hippocampus and on temporal profiles of cognitive performance of four-month-old males Holtzman rats. Groups were defined as: control (CO) and Aβ-injected (Aβ). Rats were maintained under 12 h-light:12 h-dark conditions and received food and water ad libitum throughout the entire experimental. Daily rhythms of Bax and Bcl-2 expression were analyzed by RT-PCR and protein levels by Western blots, in hippocampus samples isolated every 4 h during a 24 h period. Regulatory regions of Bax and Bcl-2 were scanned for E-box sites. The Novel Object Recognition (NOR) test was used to evaluate cognition, particularly recognition memory. We found E-box sites on regulatory regions of Bax and Bcl-2 genes, which display a daily oscillation of expression in the rat hippocampus. The i.c.v. injection of Aβ (1-42) modified daily variation of Bax and Bcl-2, and clock proteins. It was observed that the group injected with Aβ explored the novel object for less time compared to the control group, during the day and night periods. Thus, elevated Aβ peptide levels might affect the temporal patterns of cognitive function and apoptotic genes, probably by altering daily rhythms of clock proteins.
Fil: Alfaro, Mauro. Universidad Nacional de San Luis; Argentina
Fil: Coria, Lucero. Universidad Nacional de San Luis; Argentina
Fil: Ledesma, Carina Mabel. Universidad Nacional de San Luis; Argentina
Fil: Castro, Andrea. Universidad Nacional de San Luis; Argentina
Fil: Mendoza, Gisela Veronica. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
XXXIX Reunión Científica Anual de la SBCuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
description Alzheimer’s disease (AD) is a neurodegenerative disorder marked by cognitive and behavioral impairment. The accumulations of amyloid-β (Aβ) peptides in the brain are believed to be associated with perturbations of synaptic function leading to cognitive deficits. The proto-oncogene Bax (Bcl-2-associated X protein) and related protein Bcl-2 (B-cell chronic lymphocytic leukemia/lymphoma-2) genes are triggers of apoptosis in Alzheimer’s disease (AD). Besides the cognitive deficit, AD patients also show alterations in their circadian rhythms. The objective of this study was to investigate the effects of an intracerebroventricular (i.c.v.) injection of amyloid beta peptide (1-42) on daily patterns of Bax and Bcl-2 expression, as well as of clock proteins in the hippocampus and on temporal profiles of cognitive performance of four-month-old males Holtzman rats. Groups were defined as: control (CO) and Aβ-injected (Aβ). Rats were maintained under 12 h-light:12 h-dark conditions and received food and water ad libitum throughout the entire experimental. Daily rhythms of Bax and Bcl-2 expression were analyzed by RT-PCR and protein levels by Western blots, in hippocampus samples isolated every 4 h during a 24 h period. Regulatory regions of Bax and Bcl-2 were scanned for E-box sites. The Novel Object Recognition (NOR) test was used to evaluate cognition, particularly recognition memory. We found E-box sites on regulatory regions of Bax and Bcl-2 genes, which display a daily oscillation of expression in the rat hippocampus. The i.c.v. injection of Aβ (1-42) modified daily variation of Bax and Bcl-2, and clock proteins. It was observed that the group injected with Aβ explored the novel object for less time compared to the control group, during the day and night periods. Thus, elevated Aβ peptide levels might affect the temporal patterns of cognitive function and apoptotic genes, probably by altering daily rhythms of clock proteins.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189943
Effects of experimental intracerebroventricular injection of Amylois Beta Peptide (1-42) on tha daily Bax and Bcl-2 expression en the rat hippocampus; XXXIX Reunión Científica Anual de la SBCuyo; San Luis; Argentina; 2021; 18-18
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189943
identifier_str_mv Effects of experimental intracerebroventricular injection of Amylois Beta Peptide (1-42) on tha daily Bax and Bcl-2 expression en the rat hippocampus; XXXIX Reunión Científica Anual de la SBCuyo; San Luis; Argentina; 2021; 18-18
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/resumenes-de-la-reunion-2021/
info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v46nSuppl.5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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publisher.none.fl_str_mv Biocell
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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