An intracerebroventricular injection of Aβ-amyloid (1-42) aggregates modifies temporal profiles of spatial memory in rat

Autores
Castro, Andrea Grisel; Coria Lucero, Cinthia Daiana; Ledesma, Carina Mabel; Garraza, Marisa Hilda; Anzulovich Miranda, Ana Cecilia; Delgado, Silvia Marcela; Navigatore Fonzo, Lorena Silvina
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Alzheimer’s disease (AD) is the major cause of dementia in older people. AD is clinically characterized by a progressive cognitive decline followed by loss of memory. In the present study, we aimed to investigate the consequences of an intracerebroventricular (ICV) injection of aggregated β-amyloid peptide (1-42) on temporal patterns of cognitive functions using a modified version of the Barnes maze test. Four-month-old males Holtzman rats were divided into two groups defined as: control (CO) and Aβ-injected (Aβ). Rats were maintained under 12 h-light:12 h-dark conditions and received water and food ad libitum. The first group received an intracerebroventricular injection of sterile saline solution (5 μL), and the second group received an ICV injection of the solution of Aβ (1-42) aggregated (5 μL). A week after the surgery, Barnes’ test modified was performed to test cognitive functions of the rats during day and night. The parameters assessed were as follows: (a) number of errors; (b) escape box latency; (c) Hole exploration frequency in the goal sector; (d) Total exploratory activity; (e) Path length. In the behavioral studies we used 23 animals from each control group and Aβ-injected groups. Our results demonstrated that Aβ-injected rats showed an increase in the number of errors to reach the target hole at night and exhibited a notable increase in the latent period during day and night, in comparison with control group. During the day, the Aβ-injected group explored less frequently the target sector and in night showed a significantly lower exploratory activity. The total distance walked was affected by the ICV injection of the amyloid aggregates. According to these results, we could suggest that the temporal patterns of spatial memory are altered in animals injected with Aβ and are correlated with the increase in Aβ observed by our group.
Fil: Castro, Andrea Grisel. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Coria Lucero, Cinthia Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Ledesma, Carina Mabel. Universidad Nacional de San Luis; Argentina
Fil: Garraza, Marisa Hilda. Universidad Nacional de San Luis; Argentina
Fil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Delgado, Silvia Marcela. Universidad Nacional de San Luis; Argentina
Fil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
XXXIX Annual Meeting of Sociedad de Biología de Cuyo
Argentina
Sociedad de Biología de Cuyo
Materia
INTRACEREBROVENTRICULAR INJECTION OF Aβ
TEMPORAL PROFILES
SPATIAL MEMORY
RAT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194681
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/194681
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An intracerebroventricular injection of Aβ-amyloid (1-42) aggregates modifies temporal profiles of spatial memory in ratCastro, Andrea GriselCoria Lucero, Cinthia DaianaLedesma, Carina MabelGarraza, Marisa HildaAnzulovich Miranda, Ana CeciliaDelgado, Silvia MarcelaNavigatore Fonzo, Lorena SilvinaINTRACEREBROVENTRICULAR INJECTION OF AβTEMPORAL PROFILESSPATIAL MEMORYRAThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Alzheimer’s disease (AD) is the major cause of dementia in older people. AD is clinically characterized by a progressive cognitive decline followed by loss of memory. In the present study, we aimed to investigate the consequences of an intracerebroventricular (ICV) injection of aggregated β-amyloid peptide (1-42) on temporal patterns of cognitive functions using a modified version of the Barnes maze test. Four-month-old males Holtzman rats were divided into two groups defined as: control (CO) and Aβ-injected (Aβ). Rats were maintained under 12 h-light:12 h-dark conditions and received water and food ad libitum. The first group received an intracerebroventricular injection of sterile saline solution (5 μL), and the second group received an ICV injection of the solution of Aβ (1-42) aggregated (5 μL). A week after the surgery, Barnes’ test modified was performed to test cognitive functions of the rats during day and night. The parameters assessed were as follows: (a) number of errors; (b) escape box latency; (c) Hole exploration frequency in the goal sector; (d) Total exploratory activity; (e) Path length. In the behavioral studies we used 23 animals from each control group and Aβ-injected groups. Our results demonstrated that Aβ-injected rats showed an increase in the number of errors to reach the target hole at night and exhibited a notable increase in the latent period during day and night, in comparison with control group. During the day, the Aβ-injected group explored less frequently the target sector and in night showed a significantly lower exploratory activity. The total distance walked was affected by the ICV injection of the amyloid aggregates. According to these results, we could suggest that the temporal patterns of spatial memory are altered in animals injected with Aβ and are correlated with the increase in Aβ observed by our group.Fil: Castro, Andrea Grisel. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Coria Lucero, Cinthia Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Ledesma, Carina Mabel. Universidad Nacional de San Luis; ArgentinaFil: Garraza, Marisa Hilda. Universidad Nacional de San Luis; ArgentinaFil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Delgado, Silvia Marcela. Universidad Nacional de San Luis; ArgentinaFil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaXXXIX Annual Meeting of Sociedad de Biología de CuyoArgentinaSociedad de Biología de CuyoBiocellGomez, Nidia NoemíCastro, Claudia Magdalena2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194681An intracerebroventricular injection of Aβ-amyloid (1-42) aggregates modifies temporal profiles of spatial memory in rat; XXXIX Annual Meeting of Sociedad de Biología de Cuyo; Argentina; 2021; 49-490327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v46nSuppl.5info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/resumenes-de-la-reunion-2021/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:17:53Zoai:ri.conicet.gov.ar:11336/194681instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:17:53.828CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An intracerebroventricular injection of Aβ-amyloid (1-42) aggregates modifies temporal profiles of spatial memory in rat
title An intracerebroventricular injection of Aβ-amyloid (1-42) aggregates modifies temporal profiles of spatial memory in rat
spellingShingle An intracerebroventricular injection of Aβ-amyloid (1-42) aggregates modifies temporal profiles of spatial memory in rat
Castro, Andrea Grisel
INTRACEREBROVENTRICULAR INJECTION OF Aβ
TEMPORAL PROFILES
SPATIAL MEMORY
RAT
title_short An intracerebroventricular injection of Aβ-amyloid (1-42) aggregates modifies temporal profiles of spatial memory in rat
title_full An intracerebroventricular injection of Aβ-amyloid (1-42) aggregates modifies temporal profiles of spatial memory in rat
title_fullStr An intracerebroventricular injection of Aβ-amyloid (1-42) aggregates modifies temporal profiles of spatial memory in rat
title_full_unstemmed An intracerebroventricular injection of Aβ-amyloid (1-42) aggregates modifies temporal profiles of spatial memory in rat
title_sort An intracerebroventricular injection of Aβ-amyloid (1-42) aggregates modifies temporal profiles of spatial memory in rat
dc.creator.none.fl_str_mv Castro, Andrea Grisel
Coria Lucero, Cinthia Daiana
Ledesma, Carina Mabel
Garraza, Marisa Hilda
Anzulovich Miranda, Ana Cecilia
Delgado, Silvia Marcela
Navigatore Fonzo, Lorena Silvina
author Castro, Andrea Grisel
author_facet Castro, Andrea Grisel
Coria Lucero, Cinthia Daiana
Ledesma, Carina Mabel
Garraza, Marisa Hilda
Anzulovich Miranda, Ana Cecilia
Delgado, Silvia Marcela
Navigatore Fonzo, Lorena Silvina
author_role author
author2 Coria Lucero, Cinthia Daiana
Ledesma, Carina Mabel
Garraza, Marisa Hilda
Anzulovich Miranda, Ana Cecilia
Delgado, Silvia Marcela
Navigatore Fonzo, Lorena Silvina
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Gomez, Nidia Noemí
Castro, Claudia Magdalena
dc.subject.none.fl_str_mv INTRACEREBROVENTRICULAR INJECTION OF Aβ
TEMPORAL PROFILES
SPATIAL MEMORY
RAT
topic INTRACEREBROVENTRICULAR INJECTION OF Aβ
TEMPORAL PROFILES
SPATIAL MEMORY
RAT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Alzheimer’s disease (AD) is the major cause of dementia in older people. AD is clinically characterized by a progressive cognitive decline followed by loss of memory. In the present study, we aimed to investigate the consequences of an intracerebroventricular (ICV) injection of aggregated β-amyloid peptide (1-42) on temporal patterns of cognitive functions using a modified version of the Barnes maze test. Four-month-old males Holtzman rats were divided into two groups defined as: control (CO) and Aβ-injected (Aβ). Rats were maintained under 12 h-light:12 h-dark conditions and received water and food ad libitum. The first group received an intracerebroventricular injection of sterile saline solution (5 μL), and the second group received an ICV injection of the solution of Aβ (1-42) aggregated (5 μL). A week after the surgery, Barnes’ test modified was performed to test cognitive functions of the rats during day and night. The parameters assessed were as follows: (a) number of errors; (b) escape box latency; (c) Hole exploration frequency in the goal sector; (d) Total exploratory activity; (e) Path length. In the behavioral studies we used 23 animals from each control group and Aβ-injected groups. Our results demonstrated that Aβ-injected rats showed an increase in the number of errors to reach the target hole at night and exhibited a notable increase in the latent period during day and night, in comparison with control group. During the day, the Aβ-injected group explored less frequently the target sector and in night showed a significantly lower exploratory activity. The total distance walked was affected by the ICV injection of the amyloid aggregates. According to these results, we could suggest that the temporal patterns of spatial memory are altered in animals injected with Aβ and are correlated with the increase in Aβ observed by our group.
Fil: Castro, Andrea Grisel. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Coria Lucero, Cinthia Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Ledesma, Carina Mabel. Universidad Nacional de San Luis; Argentina
Fil: Garraza, Marisa Hilda. Universidad Nacional de San Luis; Argentina
Fil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Delgado, Silvia Marcela. Universidad Nacional de San Luis; Argentina
Fil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
XXXIX Annual Meeting of Sociedad de Biología de Cuyo
Argentina
Sociedad de Biología de Cuyo
description Alzheimer’s disease (AD) is the major cause of dementia in older people. AD is clinically characterized by a progressive cognitive decline followed by loss of memory. In the present study, we aimed to investigate the consequences of an intracerebroventricular (ICV) injection of aggregated β-amyloid peptide (1-42) on temporal patterns of cognitive functions using a modified version of the Barnes maze test. Four-month-old males Holtzman rats were divided into two groups defined as: control (CO) and Aβ-injected (Aβ). Rats were maintained under 12 h-light:12 h-dark conditions and received water and food ad libitum. The first group received an intracerebroventricular injection of sterile saline solution (5 μL), and the second group received an ICV injection of the solution of Aβ (1-42) aggregated (5 μL). A week after the surgery, Barnes’ test modified was performed to test cognitive functions of the rats during day and night. The parameters assessed were as follows: (a) number of errors; (b) escape box latency; (c) Hole exploration frequency in the goal sector; (d) Total exploratory activity; (e) Path length. In the behavioral studies we used 23 animals from each control group and Aβ-injected groups. Our results demonstrated that Aβ-injected rats showed an increase in the number of errors to reach the target hole at night and exhibited a notable increase in the latent period during day and night, in comparison with control group. During the day, the Aβ-injected group explored less frequently the target sector and in night showed a significantly lower exploratory activity. The total distance walked was affected by the ICV injection of the amyloid aggregates. According to these results, we could suggest that the temporal patterns of spatial memory are altered in animals injected with Aβ and are correlated with the increase in Aβ observed by our group.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194681
An intracerebroventricular injection of Aβ-amyloid (1-42) aggregates modifies temporal profiles of spatial memory in rat; XXXIX Annual Meeting of Sociedad de Biología de Cuyo; Argentina; 2021; 49-49
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194681
identifier_str_mv An intracerebroventricular injection of Aβ-amyloid (1-42) aggregates modifies temporal profiles of spatial memory in rat; XXXIX Annual Meeting of Sociedad de Biología de Cuyo; Argentina; 2021; 49-49
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/resumenes-de-la-reunion-2021/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
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application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Biocell
publisher.none.fl_str_mv Biocell
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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