Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular grafts

Autores
Caracciolo, Pablo Christian; Diaz Rodriguez, Patricia; Ardao, Inés; Moreira, David; Montini Ballarin, Florencia; Abraham, Gustavo Abel; Concheiro, Angel; Alvarez Lorenzo, Carmen
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
One of the main challenges of cardiovascular tissue engineering is the development of bioresorbable and compliant small-diameter vascular grafts (SDVG) for patients where autologous grafts are not an option. In this work, electrospun bilayered bioresorbable SDVG based on blends of poly(L-lactic acid) (PLLA) and segmented polyurethane (PHD) were prepared and evaluated. The inner layer of these SDVG was surface-modified with heparin, following a methodology involving PHD urethane functional groups. Heparin was selected as anticoagulant agent, and also due to its ability to promote human umbilical vein endothelial cells (HUVECs) growth and to inhibit smooth muscle cells over-proliferation, main cause of neointimal hyperplasia and restenosis. Immobilized heparin was quantified and changes in SDVG microstructure were investigated through SEM. Tensile properties of the heparin-functionalized SDVG resembled those of saphenous vein. Vascular grafts were seeded with HUVECs and cultured on a flow-perfusion bioreactor to analyze the effect of heparin on graft endothelization under simulated physiological-like conditions. The analysis of endothelial cells attachment and gene expression (Real-Time PCR) pointed out that the surface functionalization with heparin successfully promoted a stable and functional endothelial cell layer.
Fil: Caracciolo, Pablo Christian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Diaz Rodriguez, Patricia. Universidad de Santiago de Compostela; España
Fil: Ardao, Inés. Universidad de Santiago de Compostela; España
Fil: Moreira, David. Universidad de Santiago de Compostela; España
Fil: Montini Ballarin, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Concheiro, Angel. Universidad de Santiago de Compostela; España
Fil: Alvarez Lorenzo, Carmen. Universidad de Santiago de Compostela; España
Materia
BIOLOGICAL CHARACTERIZATION
BIORESORBABLE VASCULAR GRAFTS
HEPARIN
HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS (HUVECS)
SURFACE MODIFICATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/182631
Acceder
id CONICETDig_24eb81d0182e007b17b618dc4256ea34
oai_identifier_str oai:ri.conicet.gov.ar:11336/182631
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular graftsCaracciolo, Pablo ChristianDiaz Rodriguez, PatriciaArdao, InésMoreira, DavidMontini Ballarin, FlorenciaAbraham, Gustavo AbelConcheiro, AngelAlvarez Lorenzo, CarmenBIOLOGICAL CHARACTERIZATIONBIORESORBABLE VASCULAR GRAFTSHEPARINHUMAN UMBILICAL VEIN ENDOTHELIAL CELLS (HUVECS)SURFACE MODIFICATIONhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2One of the main challenges of cardiovascular tissue engineering is the development of bioresorbable and compliant small-diameter vascular grafts (SDVG) for patients where autologous grafts are not an option. In this work, electrospun bilayered bioresorbable SDVG based on blends of poly(L-lactic acid) (PLLA) and segmented polyurethane (PHD) were prepared and evaluated. The inner layer of these SDVG was surface-modified with heparin, following a methodology involving PHD urethane functional groups. Heparin was selected as anticoagulant agent, and also due to its ability to promote human umbilical vein endothelial cells (HUVECs) growth and to inhibit smooth muscle cells over-proliferation, main cause of neointimal hyperplasia and restenosis. Immobilized heparin was quantified and changes in SDVG microstructure were investigated through SEM. Tensile properties of the heparin-functionalized SDVG resembled those of saphenous vein. Vascular grafts were seeded with HUVECs and cultured on a flow-perfusion bioreactor to analyze the effect of heparin on graft endothelization under simulated physiological-like conditions. The analysis of endothelial cells attachment and gene expression (Real-Time PCR) pointed out that the surface functionalization with heparin successfully promoted a stable and functional endothelial cell layer.Fil: Caracciolo, Pablo Christian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Diaz Rodriguez, Patricia. Universidad de Santiago de Compostela; EspañaFil: Ardao, Inés. Universidad de Santiago de Compostela; EspañaFil: Moreira, David. Universidad de Santiago de Compostela; EspañaFil: Montini Ballarin, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Concheiro, Angel. Universidad de Santiago de Compostela; EspañaFil: Alvarez Lorenzo, Carmen. Universidad de Santiago de Compostela; EspañaElsevier Science2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/182631Caracciolo, Pablo Christian; Diaz Rodriguez, Patricia; Ardao, Inés; Moreira, David; Montini Ballarin, Florencia; et al.; Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular grafts; Elsevier Science; International Journal of Biological Macromolecules; 179; 3-2021; 567-5750141-8130CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0141813021005250info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2021.03.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:31Zoai:ri.conicet.gov.ar:11336/182631instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:32.21CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular grafts
title Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular grafts
spellingShingle Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular grafts
Caracciolo, Pablo Christian
BIOLOGICAL CHARACTERIZATION
BIORESORBABLE VASCULAR GRAFTS
HEPARIN
HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS (HUVECS)
SURFACE MODIFICATION
title_short Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular grafts
title_full Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular grafts
title_fullStr Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular grafts
title_full_unstemmed Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular grafts
title_sort Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular grafts
dc.creator.none.fl_str_mv Caracciolo, Pablo Christian
Diaz Rodriguez, Patricia
Ardao, Inés
Moreira, David
Montini Ballarin, Florencia
Abraham, Gustavo Abel
Concheiro, Angel
Alvarez Lorenzo, Carmen
author Caracciolo, Pablo Christian
author_facet Caracciolo, Pablo Christian
Diaz Rodriguez, Patricia
Ardao, Inés
Moreira, David
Montini Ballarin, Florencia
Abraham, Gustavo Abel
Concheiro, Angel
Alvarez Lorenzo, Carmen
author_role author
author2 Diaz Rodriguez, Patricia
Ardao, Inés
Moreira, David
Montini Ballarin, Florencia
Abraham, Gustavo Abel
Concheiro, Angel
Alvarez Lorenzo, Carmen
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOLOGICAL CHARACTERIZATION
BIORESORBABLE VASCULAR GRAFTS
HEPARIN
HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS (HUVECS)
SURFACE MODIFICATION
topic BIOLOGICAL CHARACTERIZATION
BIORESORBABLE VASCULAR GRAFTS
HEPARIN
HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS (HUVECS)
SURFACE MODIFICATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv One of the main challenges of cardiovascular tissue engineering is the development of bioresorbable and compliant small-diameter vascular grafts (SDVG) for patients where autologous grafts are not an option. In this work, electrospun bilayered bioresorbable SDVG based on blends of poly(L-lactic acid) (PLLA) and segmented polyurethane (PHD) were prepared and evaluated. The inner layer of these SDVG was surface-modified with heparin, following a methodology involving PHD urethane functional groups. Heparin was selected as anticoagulant agent, and also due to its ability to promote human umbilical vein endothelial cells (HUVECs) growth and to inhibit smooth muscle cells over-proliferation, main cause of neointimal hyperplasia and restenosis. Immobilized heparin was quantified and changes in SDVG microstructure were investigated through SEM. Tensile properties of the heparin-functionalized SDVG resembled those of saphenous vein. Vascular grafts were seeded with HUVECs and cultured on a flow-perfusion bioreactor to analyze the effect of heparin on graft endothelization under simulated physiological-like conditions. The analysis of endothelial cells attachment and gene expression (Real-Time PCR) pointed out that the surface functionalization with heparin successfully promoted a stable and functional endothelial cell layer.
Fil: Caracciolo, Pablo Christian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Diaz Rodriguez, Patricia. Universidad de Santiago de Compostela; España
Fil: Ardao, Inés. Universidad de Santiago de Compostela; España
Fil: Moreira, David. Universidad de Santiago de Compostela; España
Fil: Montini Ballarin, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Concheiro, Angel. Universidad de Santiago de Compostela; España
Fil: Alvarez Lorenzo, Carmen. Universidad de Santiago de Compostela; España
description One of the main challenges of cardiovascular tissue engineering is the development of bioresorbable and compliant small-diameter vascular grafts (SDVG) for patients where autologous grafts are not an option. In this work, electrospun bilayered bioresorbable SDVG based on blends of poly(L-lactic acid) (PLLA) and segmented polyurethane (PHD) were prepared and evaluated. The inner layer of these SDVG was surface-modified with heparin, following a methodology involving PHD urethane functional groups. Heparin was selected as anticoagulant agent, and also due to its ability to promote human umbilical vein endothelial cells (HUVECs) growth and to inhibit smooth muscle cells over-proliferation, main cause of neointimal hyperplasia and restenosis. Immobilized heparin was quantified and changes in SDVG microstructure were investigated through SEM. Tensile properties of the heparin-functionalized SDVG resembled those of saphenous vein. Vascular grafts were seeded with HUVECs and cultured on a flow-perfusion bioreactor to analyze the effect of heparin on graft endothelization under simulated physiological-like conditions. The analysis of endothelial cells attachment and gene expression (Real-Time PCR) pointed out that the surface functionalization with heparin successfully promoted a stable and functional endothelial cell layer.
publishDate 2021
dc.date.none.fl_str_mv 2021-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/182631
Caracciolo, Pablo Christian; Diaz Rodriguez, Patricia; Ardao, Inés; Moreira, David; Montini Ballarin, Florencia; et al.; Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular grafts; Elsevier Science; International Journal of Biological Macromolecules; 179; 3-2021; 567-575
0141-8130
CONICET Digital
CONICET
url http://hdl.handle.net/11336/182631
identifier_str_mv Caracciolo, Pablo Christian; Diaz Rodriguez, Patricia; Ardao, Inés; Moreira, David; Montini Ballarin, Florencia; et al.; Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular grafts; Elsevier Science; International Journal of Biological Macromolecules; 179; 3-2021; 567-575
0141-8130
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0141813021005250
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2021.03.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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