Internal mammary artery grafts reactivity in hypertensive patients: Role of stretching in extraendothelial nitric oxide

Autores
Joo Turoni, Claudio Martín; Peral, Maria de Los Angeles; Coviello, Alfredo; Marañón, Rodrigo Oscar; Herrera, Ramon Nicasio Gerardo; Muntaner, Juan; Proto, Victor
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background. The internal mammary artery (IMA) used in bypass coronary surgery remains efficient for a longer time than other grafts, such as saphenous veins; however, its biological characteristics are incompletely defined. Objective. To compare in IMA grafts from hypertensive (HT) and normotensive (NT) patients the presence of endothelium and their functionability, the response to passive stretching and basal tone, the reactivity to exogenous vasoconstrictors, the role of stretching in NO release, and the possible extraendothelial NO source. Methods and Results. IMA rings contractility, presence of endothelium, and nitrite release were studied. An endothelial dysfunction associated with hypertension was found. IMA rings from HT had an impaired response to passive stretching, resulting in a decreased relaxation. All IMA grafts had an increased basal tone demonstrated by relaxation to SNP; however, a lesser response was found in HT. Interestingly, it was demonstrated that NO release was present in IMA grafts, despite an endothelial dysfunction and that stretching increased NO release. This effect was inhibited by Ca2+-free media, L-NAME and a specific neuronal NO synthase (nNOS) inhibitor. Furthermore, the demonstration of the presence of nNOS in smooth muscle cells by immunohistochemistry supports a role of extraendothelial NO. Conclusion. We demonstrate the impact of hypertension in IMA grafts producing increased endothelial dysfunction, reduced response to passive stretching, increased basal tone, and impaired responsiveness to exogenous vasoconstrictors and NO release. A specific role of stretching in extraendothelial NO release was demonstrated, which may have an important role in the outcome of IMA grafts due to the protective actions of NO, even in the absence of the endothelium.
Fil: Joo Turoni, Claudio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Coviello, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Marañón, Rodrigo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Herrera, Ramon Nicasio Gerardo. Centro Modelo de Cardiología; Argentina
Fil: Muntaner, Juan. Centro Modelo de Cardiología; Argentina
Fil: Proto, Victor. Centro Modelo de Cardiología; Argentina
Materia
ENDOTHELIAL DYSFUNCTION
EXTRAENDOTHELIAL NITRIC OXIDE SYNTHASE
HYPERTENSION
IMA GRAFTS
VASCULAR REACTIVITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95242
Acceder
id CONICETDig_1a51cf6cf4621d173528678cb2996adf
oai_identifier_str oai:ri.conicet.gov.ar:11336/95242
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Internal mammary artery grafts reactivity in hypertensive patients: Role of stretching in extraendothelial nitric oxideJoo Turoni, Claudio MartínPeral, Maria de Los AngelesCoviello, AlfredoMarañón, Rodrigo OscarHerrera, Ramon Nicasio GerardoMuntaner, JuanProto, VictorENDOTHELIAL DYSFUNCTIONEXTRAENDOTHELIAL NITRIC OXIDE SYNTHASEHYPERTENSIONIMA GRAFTSVASCULAR REACTIVITYhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Background. The internal mammary artery (IMA) used in bypass coronary surgery remains efficient for a longer time than other grafts, such as saphenous veins; however, its biological characteristics are incompletely defined. Objective. To compare in IMA grafts from hypertensive (HT) and normotensive (NT) patients the presence of endothelium and their functionability, the response to passive stretching and basal tone, the reactivity to exogenous vasoconstrictors, the role of stretching in NO release, and the possible extraendothelial NO source. Methods and Results. IMA rings contractility, presence of endothelium, and nitrite release were studied. An endothelial dysfunction associated with hypertension was found. IMA rings from HT had an impaired response to passive stretching, resulting in a decreased relaxation. All IMA grafts had an increased basal tone demonstrated by relaxation to SNP; however, a lesser response was found in HT. Interestingly, it was demonstrated that NO release was present in IMA grafts, despite an endothelial dysfunction and that stretching increased NO release. This effect was inhibited by Ca2+-free media, L-NAME and a specific neuronal NO synthase (nNOS) inhibitor. Furthermore, the demonstration of the presence of nNOS in smooth muscle cells by immunohistochemistry supports a role of extraendothelial NO. Conclusion. We demonstrate the impact of hypertension in IMA grafts producing increased endothelial dysfunction, reduced response to passive stretching, increased basal tone, and impaired responsiveness to exogenous vasoconstrictors and NO release. A specific role of stretching in extraendothelial NO release was demonstrated, which may have an important role in the outcome of IMA grafts due to the protective actions of NO, even in the absence of the endothelium.Fil: Joo Turoni, Claudio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Coviello, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Marañón, Rodrigo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Herrera, Ramon Nicasio Gerardo. Centro Modelo de Cardiología; ArgentinaFil: Muntaner, Juan. Centro Modelo de Cardiología; ArgentinaFil: Proto, Victor. Centro Modelo de Cardiología; ArgentinaTaylor & Francis2007-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95242Joo Turoni, Claudio Martín; Peral, Maria de Los Angeles; Coviello, Alfredo; Marañón, Rodrigo Oscar; Herrera, Ramon Nicasio Gerardo; et al.; Internal mammary artery grafts reactivity in hypertensive patients: Role of stretching in extraendothelial nitric oxide; Taylor & Francis; Clinical and Experimental Hypertension; 29; 6-2007; 327-3441064-1963CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/10641960701500570info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/10641960701500570info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:00Zoai:ri.conicet.gov.ar:11336/95242instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:00.928CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Internal mammary artery grafts reactivity in hypertensive patients: Role of stretching in extraendothelial nitric oxide
title Internal mammary artery grafts reactivity in hypertensive patients: Role of stretching in extraendothelial nitric oxide
spellingShingle Internal mammary artery grafts reactivity in hypertensive patients: Role of stretching in extraendothelial nitric oxide
Joo Turoni, Claudio Martín
ENDOTHELIAL DYSFUNCTION
EXTRAENDOTHELIAL NITRIC OXIDE SYNTHASE
HYPERTENSION
IMA GRAFTS
VASCULAR REACTIVITY
title_short Internal mammary artery grafts reactivity in hypertensive patients: Role of stretching in extraendothelial nitric oxide
title_full Internal mammary artery grafts reactivity in hypertensive patients: Role of stretching in extraendothelial nitric oxide
title_fullStr Internal mammary artery grafts reactivity in hypertensive patients: Role of stretching in extraendothelial nitric oxide
title_full_unstemmed Internal mammary artery grafts reactivity in hypertensive patients: Role of stretching in extraendothelial nitric oxide
title_sort Internal mammary artery grafts reactivity in hypertensive patients: Role of stretching in extraendothelial nitric oxide
dc.creator.none.fl_str_mv Joo Turoni, Claudio Martín
Peral, Maria de Los Angeles
Coviello, Alfredo
Marañón, Rodrigo Oscar
Herrera, Ramon Nicasio Gerardo
Muntaner, Juan
Proto, Victor
author Joo Turoni, Claudio Martín
author_facet Joo Turoni, Claudio Martín
Peral, Maria de Los Angeles
Coviello, Alfredo
Marañón, Rodrigo Oscar
Herrera, Ramon Nicasio Gerardo
Muntaner, Juan
Proto, Victor
author_role author
author2 Peral, Maria de Los Angeles
Coviello, Alfredo
Marañón, Rodrigo Oscar
Herrera, Ramon Nicasio Gerardo
Muntaner, Juan
Proto, Victor
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ENDOTHELIAL DYSFUNCTION
EXTRAENDOTHELIAL NITRIC OXIDE SYNTHASE
HYPERTENSION
IMA GRAFTS
VASCULAR REACTIVITY
topic ENDOTHELIAL DYSFUNCTION
EXTRAENDOTHELIAL NITRIC OXIDE SYNTHASE
HYPERTENSION
IMA GRAFTS
VASCULAR REACTIVITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background. The internal mammary artery (IMA) used in bypass coronary surgery remains efficient for a longer time than other grafts, such as saphenous veins; however, its biological characteristics are incompletely defined. Objective. To compare in IMA grafts from hypertensive (HT) and normotensive (NT) patients the presence of endothelium and their functionability, the response to passive stretching and basal tone, the reactivity to exogenous vasoconstrictors, the role of stretching in NO release, and the possible extraendothelial NO source. Methods and Results. IMA rings contractility, presence of endothelium, and nitrite release were studied. An endothelial dysfunction associated with hypertension was found. IMA rings from HT had an impaired response to passive stretching, resulting in a decreased relaxation. All IMA grafts had an increased basal tone demonstrated by relaxation to SNP; however, a lesser response was found in HT. Interestingly, it was demonstrated that NO release was present in IMA grafts, despite an endothelial dysfunction and that stretching increased NO release. This effect was inhibited by Ca2+-free media, L-NAME and a specific neuronal NO synthase (nNOS) inhibitor. Furthermore, the demonstration of the presence of nNOS in smooth muscle cells by immunohistochemistry supports a role of extraendothelial NO. Conclusion. We demonstrate the impact of hypertension in IMA grafts producing increased endothelial dysfunction, reduced response to passive stretching, increased basal tone, and impaired responsiveness to exogenous vasoconstrictors and NO release. A specific role of stretching in extraendothelial NO release was demonstrated, which may have an important role in the outcome of IMA grafts due to the protective actions of NO, even in the absence of the endothelium.
Fil: Joo Turoni, Claudio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Coviello, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Marañón, Rodrigo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Herrera, Ramon Nicasio Gerardo. Centro Modelo de Cardiología; Argentina
Fil: Muntaner, Juan. Centro Modelo de Cardiología; Argentina
Fil: Proto, Victor. Centro Modelo de Cardiología; Argentina
description Background. The internal mammary artery (IMA) used in bypass coronary surgery remains efficient for a longer time than other grafts, such as saphenous veins; however, its biological characteristics are incompletely defined. Objective. To compare in IMA grafts from hypertensive (HT) and normotensive (NT) patients the presence of endothelium and their functionability, the response to passive stretching and basal tone, the reactivity to exogenous vasoconstrictors, the role of stretching in NO release, and the possible extraendothelial NO source. Methods and Results. IMA rings contractility, presence of endothelium, and nitrite release were studied. An endothelial dysfunction associated with hypertension was found. IMA rings from HT had an impaired response to passive stretching, resulting in a decreased relaxation. All IMA grafts had an increased basal tone demonstrated by relaxation to SNP; however, a lesser response was found in HT. Interestingly, it was demonstrated that NO release was present in IMA grafts, despite an endothelial dysfunction and that stretching increased NO release. This effect was inhibited by Ca2+-free media, L-NAME and a specific neuronal NO synthase (nNOS) inhibitor. Furthermore, the demonstration of the presence of nNOS in smooth muscle cells by immunohistochemistry supports a role of extraendothelial NO. Conclusion. We demonstrate the impact of hypertension in IMA grafts producing increased endothelial dysfunction, reduced response to passive stretching, increased basal tone, and impaired responsiveness to exogenous vasoconstrictors and NO release. A specific role of stretching in extraendothelial NO release was demonstrated, which may have an important role in the outcome of IMA grafts due to the protective actions of NO, even in the absence of the endothelium.
publishDate 2007
dc.date.none.fl_str_mv 2007-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95242
Joo Turoni, Claudio Martín; Peral, Maria de Los Angeles; Coviello, Alfredo; Marañón, Rodrigo Oscar; Herrera, Ramon Nicasio Gerardo; et al.; Internal mammary artery grafts reactivity in hypertensive patients: Role of stretching in extraendothelial nitric oxide; Taylor & Francis; Clinical and Experimental Hypertension; 29; 6-2007; 327-344
1064-1963
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95242
identifier_str_mv Joo Turoni, Claudio Martín; Peral, Maria de Los Angeles; Coviello, Alfredo; Marañón, Rodrigo Oscar; Herrera, Ramon Nicasio Gerardo; et al.; Internal mammary artery grafts reactivity in hypertensive patients: Role of stretching in extraendothelial nitric oxide; Taylor & Francis; Clinical and Experimental Hypertension; 29; 6-2007; 327-344
1064-1963
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/10641960701500570
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/10641960701500570
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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