A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation Method

Autores
Zumbardo Bacelis, Gualberto Antonio; Peponi, Laura; Vargas Coronado, Rossana Faride; Rodríguez Velázquez, Eustolia; Alatorre Meda, Manuel; Chevallier, Pascale; Copes, Francesco; Mantovani, Diego; Abraham, Gustavo Abel; Cauich Rodríguez, Juan Valerio
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study used the roto-evaporation technique to engineer a 6 mm three-layer polyurethane vascular graft (TVG) that mimics the architecture of human coronary artery native vessels. Two segmented polyurethanes were synthesized using lysine (SPUUK) and ascorbic acid (SPUAA), and the resulting materials were used to create the intima and adventitia layers, respectively. In contrast, the media layer of the TVG was composed of a commercially available polyurethane, Pearlbond 703 EXP. For comparison purposes, single-layer vascular grafts (SVGs) from individual polyurethanes and a polyurethane blend (MVG) were made and tested similarly and evaluated according to the ISO 7198 standard. The TVG exhibited the highest circumferential tensile strength and longitudinal forces compared to single-layer vascular grafts of lower thicknesses made from the same polyurethanes. The TVG also showed higher suture and burst strength values than native vessels. The TVG withstood up to 2087 ± 139 mmHg and exhibited a compliance of 0.15 ± 0.1%/100 mmHg, while SPUUK SVGs showed a compliance of 5.21 ± 1.29%/100 mmHg, akin to coronary arteries but superior to the saphenous vein. An indirect cytocompatibility test using the MDA-MB-231 cell line showed 90 to 100% viability for all polyurethanes, surpassing the minimum 70% threshold needed for biomaterials deemed cytocompatibility. Despite the non-cytotoxic nature of the polyurethane extracts when grown directly on the surface, they displayed poor fibroblast adhesion, except for SPUUK. All vascular grafts showed hemolysis values under the permissible limit of 5% and longer coagulation times.
Fil: Zumbardo Bacelis, Gualberto Antonio. Laval University; Canadá
Fil: Peponi, Laura. Consejo Superior de Investigaciones Científicas; España
Fil: Vargas Coronado, Rossana Faride. No especifíca;
Fil: Rodríguez Velázquez, Eustolia. Universidad Autonoma de Baja California (universidad Baja California);
Fil: Alatorre Meda, Manuel. Instituto Tecnológico de Tijuana; México
Fil: Chevallier, Pascale. Laval University; Canadá
Fil: Copes, Francesco. Laval University; Canadá
Fil: Mantovani, Diego. Laval University; Canadá
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Cauich Rodríguez, Juan Valerio. No especifíca;
Materia
POLYURETHANES
VASCULAR GRAFTS
SYNTHESIS
MECHANICAL PROPERTIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243405
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/243405
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation MethodZumbardo Bacelis, Gualberto AntonioPeponi, LauraVargas Coronado, Rossana FarideRodríguez Velázquez, EustoliaAlatorre Meda, ManuelChevallier, PascaleCopes, FrancescoMantovani, DiegoAbraham, Gustavo AbelCauich Rodríguez, Juan ValerioPOLYURETHANESVASCULAR GRAFTSSYNTHESISMECHANICAL PROPERTIEShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2This study used the roto-evaporation technique to engineer a 6 mm three-layer polyurethane vascular graft (TVG) that mimics the architecture of human coronary artery native vessels. Two segmented polyurethanes were synthesized using lysine (SPUUK) and ascorbic acid (SPUAA), and the resulting materials were used to create the intima and adventitia layers, respectively. In contrast, the media layer of the TVG was composed of a commercially available polyurethane, Pearlbond 703 EXP. For comparison purposes, single-layer vascular grafts (SVGs) from individual polyurethanes and a polyurethane blend (MVG) were made and tested similarly and evaluated according to the ISO 7198 standard. The TVG exhibited the highest circumferential tensile strength and longitudinal forces compared to single-layer vascular grafts of lower thicknesses made from the same polyurethanes. The TVG also showed higher suture and burst strength values than native vessels. The TVG withstood up to 2087 ± 139 mmHg and exhibited a compliance of 0.15 ± 0.1%/100 mmHg, while SPUUK SVGs showed a compliance of 5.21 ± 1.29%/100 mmHg, akin to coronary arteries but superior to the saphenous vein. An indirect cytocompatibility test using the MDA-MB-231 cell line showed 90 to 100% viability for all polyurethanes, surpassing the minimum 70% threshold needed for biomaterials deemed cytocompatibility. Despite the non-cytotoxic nature of the polyurethane extracts when grown directly on the surface, they displayed poor fibroblast adhesion, except for SPUUK. All vascular grafts showed hemolysis values under the permissible limit of 5% and longer coagulation times.Fil: Zumbardo Bacelis, Gualberto Antonio. Laval University; CanadáFil: Peponi, Laura. Consejo Superior de Investigaciones Científicas; EspañaFil: Vargas Coronado, Rossana Faride. No especifíca;Fil: Rodríguez Velázquez, Eustolia. Universidad Autonoma de Baja California (universidad Baja California);Fil: Alatorre Meda, Manuel. Instituto Tecnológico de Tijuana; MéxicoFil: Chevallier, Pascale. Laval University; CanadáFil: Copes, Francesco. Laval University; CanadáFil: Mantovani, Diego. Laval University; CanadáFil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Cauich Rodríguez, Juan Valerio. No especifíca;MDPI2024-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243405Zumbardo Bacelis, Gualberto Antonio; Peponi, Laura; Vargas Coronado, Rossana Faride; Rodríguez Velázquez, Eustolia; Alatorre Meda, Manuel; et al.; A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation Method; MDPI; Polymers; 16; 10; 5-2024; 1-232073-4360CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4360/16/10/1314info:eu-repo/semantics/altIdentifier/doi/10.3390/polym16101314info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:09Zoai:ri.conicet.gov.ar:11336/243405instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:09.984CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation Method
title A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation Method
spellingShingle A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation Method
Zumbardo Bacelis, Gualberto Antonio
POLYURETHANES
VASCULAR GRAFTS
SYNTHESIS
MECHANICAL PROPERTIES
title_short A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation Method
title_full A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation Method
title_fullStr A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation Method
title_full_unstemmed A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation Method
title_sort A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation Method
dc.creator.none.fl_str_mv Zumbardo Bacelis, Gualberto Antonio
Peponi, Laura
Vargas Coronado, Rossana Faride
Rodríguez Velázquez, Eustolia
Alatorre Meda, Manuel
Chevallier, Pascale
Copes, Francesco
Mantovani, Diego
Abraham, Gustavo Abel
Cauich Rodríguez, Juan Valerio
author Zumbardo Bacelis, Gualberto Antonio
author_facet Zumbardo Bacelis, Gualberto Antonio
Peponi, Laura
Vargas Coronado, Rossana Faride
Rodríguez Velázquez, Eustolia
Alatorre Meda, Manuel
Chevallier, Pascale
Copes, Francesco
Mantovani, Diego
Abraham, Gustavo Abel
Cauich Rodríguez, Juan Valerio
author_role author
author2 Peponi, Laura
Vargas Coronado, Rossana Faride
Rodríguez Velázquez, Eustolia
Alatorre Meda, Manuel
Chevallier, Pascale
Copes, Francesco
Mantovani, Diego
Abraham, Gustavo Abel
Cauich Rodríguez, Juan Valerio
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv POLYURETHANES
VASCULAR GRAFTS
SYNTHESIS
MECHANICAL PROPERTIES
topic POLYURETHANES
VASCULAR GRAFTS
SYNTHESIS
MECHANICAL PROPERTIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv This study used the roto-evaporation technique to engineer a 6 mm three-layer polyurethane vascular graft (TVG) that mimics the architecture of human coronary artery native vessels. Two segmented polyurethanes were synthesized using lysine (SPUUK) and ascorbic acid (SPUAA), and the resulting materials were used to create the intima and adventitia layers, respectively. In contrast, the media layer of the TVG was composed of a commercially available polyurethane, Pearlbond 703 EXP. For comparison purposes, single-layer vascular grafts (SVGs) from individual polyurethanes and a polyurethane blend (MVG) were made and tested similarly and evaluated according to the ISO 7198 standard. The TVG exhibited the highest circumferential tensile strength and longitudinal forces compared to single-layer vascular grafts of lower thicknesses made from the same polyurethanes. The TVG also showed higher suture and burst strength values than native vessels. The TVG withstood up to 2087 ± 139 mmHg and exhibited a compliance of 0.15 ± 0.1%/100 mmHg, while SPUUK SVGs showed a compliance of 5.21 ± 1.29%/100 mmHg, akin to coronary arteries but superior to the saphenous vein. An indirect cytocompatibility test using the MDA-MB-231 cell line showed 90 to 100% viability for all polyurethanes, surpassing the minimum 70% threshold needed for biomaterials deemed cytocompatibility. Despite the non-cytotoxic nature of the polyurethane extracts when grown directly on the surface, they displayed poor fibroblast adhesion, except for SPUUK. All vascular grafts showed hemolysis values under the permissible limit of 5% and longer coagulation times.
Fil: Zumbardo Bacelis, Gualberto Antonio. Laval University; Canadá
Fil: Peponi, Laura. Consejo Superior de Investigaciones Científicas; España
Fil: Vargas Coronado, Rossana Faride. No especifíca;
Fil: Rodríguez Velázquez, Eustolia. Universidad Autonoma de Baja California (universidad Baja California);
Fil: Alatorre Meda, Manuel. Instituto Tecnológico de Tijuana; México
Fil: Chevallier, Pascale. Laval University; Canadá
Fil: Copes, Francesco. Laval University; Canadá
Fil: Mantovani, Diego. Laval University; Canadá
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Cauich Rodríguez, Juan Valerio. No especifíca;
description This study used the roto-evaporation technique to engineer a 6 mm three-layer polyurethane vascular graft (TVG) that mimics the architecture of human coronary artery native vessels. Two segmented polyurethanes were synthesized using lysine (SPUUK) and ascorbic acid (SPUAA), and the resulting materials were used to create the intima and adventitia layers, respectively. In contrast, the media layer of the TVG was composed of a commercially available polyurethane, Pearlbond 703 EXP. For comparison purposes, single-layer vascular grafts (SVGs) from individual polyurethanes and a polyurethane blend (MVG) were made and tested similarly and evaluated according to the ISO 7198 standard. The TVG exhibited the highest circumferential tensile strength and longitudinal forces compared to single-layer vascular grafts of lower thicknesses made from the same polyurethanes. The TVG also showed higher suture and burst strength values than native vessels. The TVG withstood up to 2087 ± 139 mmHg and exhibited a compliance of 0.15 ± 0.1%/100 mmHg, while SPUUK SVGs showed a compliance of 5.21 ± 1.29%/100 mmHg, akin to coronary arteries but superior to the saphenous vein. An indirect cytocompatibility test using the MDA-MB-231 cell line showed 90 to 100% viability for all polyurethanes, surpassing the minimum 70% threshold needed for biomaterials deemed cytocompatibility. Despite the non-cytotoxic nature of the polyurethane extracts when grown directly on the surface, they displayed poor fibroblast adhesion, except for SPUUK. All vascular grafts showed hemolysis values under the permissible limit of 5% and longer coagulation times.
publishDate 2024
dc.date.none.fl_str_mv 2024-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243405
Zumbardo Bacelis, Gualberto Antonio; Peponi, Laura; Vargas Coronado, Rossana Faride; Rodríguez Velázquez, Eustolia; Alatorre Meda, Manuel; et al.; A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation Method; MDPI; Polymers; 16; 10; 5-2024; 1-23
2073-4360
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243405
identifier_str_mv Zumbardo Bacelis, Gualberto Antonio; Peponi, Laura; Vargas Coronado, Rossana Faride; Rodríguez Velázquez, Eustolia; Alatorre Meda, Manuel; et al.; A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation Method; MDPI; Polymers; 16; 10; 5-2024; 1-23
2073-4360
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4360/16/10/1314
info:eu-repo/semantics/altIdentifier/doi/10.3390/polym16101314
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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