Comparison of deproteinized bovine bone, bioglass and, synthetic hydroxyapatite in the bone healing of a critical sized bone defect. Preliminary study in rats.

Autores
Pellegrini, Gretel Gisela; Gonzales Chaves, Macarena Maria Sol; Orzuza, Ricardo; Zeni, Susana Noemi
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Bone grafts are important for alveolar bone height and volume preservation necessary for dental implant placement. The development of biomaterials for bone grafting with comparable characteristics and 
biological effects than those renowned internationally is necessary. Deproteinized bovine bone putty (BB), bioglass (BG) and, synthetic hydroxyapatite (SH) are frequently indicated as bone grafting materials due to their osteoconductive properties. We compared the bone healing response of BB, BG and SH (Odontit Implant Systems, Argentina) in a critical sized bone defect. We created a bone defect of 4 mm diameter in rat tibiae for implantation with each biomaterial (N=30 rats). Samples were collected at 2 and 4 weeks for histological and histomorphometrical analysis of new bone formation (NBF) and remaining particles of each device (RP). Radiographic analysis was done at T=0 and at 2 and 4 weeks. Results: % of NBF (mean ± SD): 2 weeks: Control group: 6.60±3.71; BB group: 23.23±3.89*; BG group: 18.35±5.23*; SH group: 26.27±9.30*; 4 weeks: Control group: 6.69±2.38; BB group: 24.37±3.66*; BG group: 17.45±6.64*; SH group: 32.25±3.80*. % of RP: 2 weeks: Control group: 0±0; BB group: 5.04±1.39*; BG group: 3.03±2.31*; SH group: 4.46±2.87*; 4 weeks: Control group: 0±0; BB group: 4.45±2.35*; BG group: 2.87±1.14*; SH group: 3.78±1.68* (*p<0.05 versus control group; p= NS between BB, BG and SH groups). Although SH exhibited a trend towards increased NBF we did not find statistical significance among the three biomaterials. Bone healing at the implanted sites, was accompanied by a progressive inflammatory response consistent with the expected histological stages of bone repairing. The 3 biomaterials exhibited an increased in radiopacity at 2 and at 4 weeks vs. control group indicating NBF. All biomaterials were associated with trabecular bone formation. Although further studies need to be done, our results indicate that BB, BG and SH exhibit similar osteoconductive properties. Keywords: Bone healing; biomaterials; critical sized bone defect; bovine bone; bioglass; synthetic hydroxyapatite.
Fil: Pellegrini, Gretel Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Gonzales Chaves, Macarena Maria Sol. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Orzuza, Ricardo. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina
Fil: Zeni, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental ; XXI Reunión Anual de la Sociedad Argentina de Biología; Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Asociación Argentina de Farmacología Experimental
Materia
critical sized bone defect
deproteinized bone
bioglass
synthetic hydroxyapatite
bone healing
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247609
Acceder
id CONICETDig_93a7bd0d2e5dd7b680f85e19d3e0c1ec
oai_identifier_str oai:ri.conicet.gov.ar:11336/247609
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparison of deproteinized bovine bone, bioglass and, synthetic hydroxyapatite in the bone healing of a critical sized bone defect. Preliminary study in rats.Pellegrini, Gretel GiselaGonzales Chaves, Macarena Maria SolOrzuza, RicardoZeni, Susana Noemicritical sized bone defectdeproteinized bonebioglasssynthetic hydroxyapatitebone healinghttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Bone grafts are important for alveolar bone height and volume preservation necessary for dental implant placement. The development of biomaterials for bone grafting with comparable characteristics and 
biological effects than those renowned internationally is necessary. Deproteinized bovine bone putty (BB), bioglass (BG) and, synthetic hydroxyapatite (SH) are frequently indicated as bone grafting materials due to their osteoconductive properties. We compared the bone healing response of BB, BG and SH (Odontit Implant Systems, Argentina) in a critical sized bone defect. We created a bone defect of 4 mm diameter in rat tibiae for implantation with each biomaterial (N=30 rats). Samples were collected at 2 and 4 weeks for histological and histomorphometrical analysis of new bone formation (NBF) and remaining particles of each device (RP). Radiographic analysis was done at T=0 and at 2 and 4 weeks. Results: % of NBF (mean ± SD): 2 weeks: Control group: 6.60±3.71; BB group: 23.23±3.89*; BG group: 18.35±5.23*; SH group: 26.27±9.30*; 4 weeks: Control group: 6.69±2.38; BB group: 24.37±3.66*; BG group: 17.45±6.64*; SH group: 32.25±3.80*. % of RP: 2 weeks: Control group: 0±0; BB group: 5.04±1.39*; BG group: 3.03±2.31*; SH group: 4.46±2.87*; 4 weeks: Control group: 0±0; BB group: 4.45±2.35*; BG group: 2.87±1.14*; SH group: 3.78±1.68* (*p<0.05 versus control group; p= NS between BB, BG and SH groups). Although SH exhibited a trend towards increased NBF we did not find statistical significance among the three biomaterials. Bone healing at the implanted sites, was accompanied by a progressive inflammatory response consistent with the expected histological stages of bone repairing. The 3 biomaterials exhibited an increased in radiopacity at 2 and at 4 weeks vs. control group indicating NBF. All biomaterials were associated with trabecular bone formation. Although further studies need to be done, our results indicate that BB, BG and SH exhibit similar osteoconductive properties. Keywords: Bone healing; biomaterials; critical sized bone defect; bovine bone; bioglass; synthetic hydroxyapatite.Fil: Pellegrini, Gretel Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Gonzales Chaves, Macarena Maria Sol. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Orzuza, Ricardo. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; ArgentinaFil: Zeni, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaLXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental ; XXI Reunión Anual de la Sociedad Argentina de Biología; Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioAsociación Argentina de Farmacología ExperimentalFundación Revista Medicina2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/247609Comparison of deproteinized bovine bone, bioglass and, synthetic hydroxyapatite in the bone healing of a critical sized bone defect. Preliminary study in rats.; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental ; XXI Reunión Anual de la Sociedad Argentina de Biología; Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 1-40076-6046CONICET DigitalCONICETengInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:14:59Zoai:ri.conicet.gov.ar:11336/247609instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:14:59.56CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparison of deproteinized bovine bone, bioglass and, synthetic hydroxyapatite in the bone healing of a critical sized bone defect. Preliminary study in rats.
title Comparison of deproteinized bovine bone, bioglass and, synthetic hydroxyapatite in the bone healing of a critical sized bone defect. Preliminary study in rats.
spellingShingle Comparison of deproteinized bovine bone, bioglass and, synthetic hydroxyapatite in the bone healing of a critical sized bone defect. Preliminary study in rats.
Pellegrini, Gretel Gisela
critical sized bone defect
deproteinized bone
bioglass
synthetic hydroxyapatite
bone healing
title_short Comparison of deproteinized bovine bone, bioglass and, synthetic hydroxyapatite in the bone healing of a critical sized bone defect. Preliminary study in rats.
title_full Comparison of deproteinized bovine bone, bioglass and, synthetic hydroxyapatite in the bone healing of a critical sized bone defect. Preliminary study in rats.
title_fullStr Comparison of deproteinized bovine bone, bioglass and, synthetic hydroxyapatite in the bone healing of a critical sized bone defect. Preliminary study in rats.
title_full_unstemmed Comparison of deproteinized bovine bone, bioglass and, synthetic hydroxyapatite in the bone healing of a critical sized bone defect. Preliminary study in rats.
title_sort Comparison of deproteinized bovine bone, bioglass and, synthetic hydroxyapatite in the bone healing of a critical sized bone defect. Preliminary study in rats.
dc.creator.none.fl_str_mv Pellegrini, Gretel Gisela
Gonzales Chaves, Macarena Maria Sol
Orzuza, Ricardo
Zeni, Susana Noemi
author Pellegrini, Gretel Gisela
author_facet Pellegrini, Gretel Gisela
Gonzales Chaves, Macarena Maria Sol
Orzuza, Ricardo
Zeni, Susana Noemi
author_role author
author2 Gonzales Chaves, Macarena Maria Sol
Orzuza, Ricardo
Zeni, Susana Noemi
author2_role author
author
author
dc.subject.none.fl_str_mv critical sized bone defect
deproteinized bone
bioglass
synthetic hydroxyapatite
bone healing
topic critical sized bone defect
deproteinized bone
bioglass
synthetic hydroxyapatite
bone healing
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Bone grafts are important for alveolar bone height and volume preservation necessary for dental implant placement. The development of biomaterials for bone grafting with comparable characteristics and 
biological effects than those renowned internationally is necessary. Deproteinized bovine bone putty (BB), bioglass (BG) and, synthetic hydroxyapatite (SH) are frequently indicated as bone grafting materials due to their osteoconductive properties. We compared the bone healing response of BB, BG and SH (Odontit Implant Systems, Argentina) in a critical sized bone defect. We created a bone defect of 4 mm diameter in rat tibiae for implantation with each biomaterial (N=30 rats). Samples were collected at 2 and 4 weeks for histological and histomorphometrical analysis of new bone formation (NBF) and remaining particles of each device (RP). Radiographic analysis was done at T=0 and at 2 and 4 weeks. Results: % of NBF (mean ± SD): 2 weeks: Control group: 6.60±3.71; BB group: 23.23±3.89*; BG group: 18.35±5.23*; SH group: 26.27±9.30*; 4 weeks: Control group: 6.69±2.38; BB group: 24.37±3.66*; BG group: 17.45±6.64*; SH group: 32.25±3.80*. % of RP: 2 weeks: Control group: 0±0; BB group: 5.04±1.39*; BG group: 3.03±2.31*; SH group: 4.46±2.87*; 4 weeks: Control group: 0±0; BB group: 4.45±2.35*; BG group: 2.87±1.14*; SH group: 3.78±1.68* (*p<0.05 versus control group; p= NS between BB, BG and SH groups). Although SH exhibited a trend towards increased NBF we did not find statistical significance among the three biomaterials. Bone healing at the implanted sites, was accompanied by a progressive inflammatory response consistent with the expected histological stages of bone repairing. The 3 biomaterials exhibited an increased in radiopacity at 2 and at 4 weeks vs. control group indicating NBF. All biomaterials were associated with trabecular bone formation. Although further studies need to be done, our results indicate that BB, BG and SH exhibit similar osteoconductive properties. Keywords: Bone healing; biomaterials; critical sized bone defect; bovine bone; bioglass; synthetic hydroxyapatite.
Fil: Pellegrini, Gretel Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Gonzales Chaves, Macarena Maria Sol. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Orzuza, Ricardo. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina
Fil: Zeni, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental ; XXI Reunión Anual de la Sociedad Argentina de Biología; Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Asociación Argentina de Farmacología Experimental
description Bone grafts are important for alveolar bone height and volume preservation necessary for dental implant placement. The development of biomaterials for bone grafting with comparable characteristics and 
biological effects than those renowned internationally is necessary. Deproteinized bovine bone putty (BB), bioglass (BG) and, synthetic hydroxyapatite (SH) are frequently indicated as bone grafting materials due to their osteoconductive properties. We compared the bone healing response of BB, BG and SH (Odontit Implant Systems, Argentina) in a critical sized bone defect. We created a bone defect of 4 mm diameter in rat tibiae for implantation with each biomaterial (N=30 rats). Samples were collected at 2 and 4 weeks for histological and histomorphometrical analysis of new bone formation (NBF) and remaining particles of each device (RP). Radiographic analysis was done at T=0 and at 2 and 4 weeks. Results: % of NBF (mean ± SD): 2 weeks: Control group: 6.60±3.71; BB group: 23.23±3.89*; BG group: 18.35±5.23*; SH group: 26.27±9.30*; 4 weeks: Control group: 6.69±2.38; BB group: 24.37±3.66*; BG group: 17.45±6.64*; SH group: 32.25±3.80*. % of RP: 2 weeks: Control group: 0±0; BB group: 5.04±1.39*; BG group: 3.03±2.31*; SH group: 4.46±2.87*; 4 weeks: Control group: 0±0; BB group: 4.45±2.35*; BG group: 2.87±1.14*; SH group: 3.78±1.68* (*p<0.05 versus control group; p= NS between BB, BG and SH groups). Although SH exhibited a trend towards increased NBF we did not find statistical significance among the three biomaterials. Bone healing at the implanted sites, was accompanied by a progressive inflammatory response consistent with the expected histological stages of bone repairing. The 3 biomaterials exhibited an increased in radiopacity at 2 and at 4 weeks vs. control group indicating NBF. All biomaterials were associated with trabecular bone formation. Although further studies need to be done, our results indicate that BB, BG and SH exhibit similar osteoconductive properties. Keywords: Bone healing; biomaterials; critical sized bone defect; bovine bone; bioglass; synthetic hydroxyapatite.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247609
Comparison of deproteinized bovine bone, bioglass and, synthetic hydroxyapatite in the bone healing of a critical sized bone defect. Preliminary study in rats.; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental ; XXI Reunión Anual de la Sociedad Argentina de Biología; Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 1-4
0076-6046
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247609
identifier_str_mv Comparison of deproteinized bovine bone, bioglass and, synthetic hydroxyapatite in the bone healing of a critical sized bone defect. Preliminary study in rats.; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental ; XXI Reunión Anual de la Sociedad Argentina de Biología; Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 1-4
0076-6046
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980805145526272
score 12.993085

Publicaciones similares