Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors

Autores
Joo Turoni, Claudio Martín; Marañón, Rodrigo Oscar; Proto, Victor; Herrera, Ramon Nicasio Gerardo; Peral, Maria de Los Angeles
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We investigated the effects of extraendothelial nitric oxide (NO) on angiotensin II (Ang II) reactivity in internal mammary artery (IMA) rings, as well as the impact of hypertension without associated risk factors in this response. Vascular reactivity, NO levels, and resting membrane potentials were determined in hypertensive (HT) and normotensive (NT) IMA rings. Only rings with endothelial dysfunction were included. Ang II produced a dose-dependent contraction that was higher in HT rings. Response to Ang II was potentiated by Nω-nitro L-arginine methyl ester (L-NAME) in NT but not in HT rings. The antioxidant agents tempol and diphenyleneiodonium (DPI) reverted the hyperreactivity to Ang II in HT rings. Extraendothelial NO was present in both NT and HT rings. However, NT rings showed higher values. LNAME and S-methyl-L-thiocitrulline inhibited NO release in all cases. L-arginine reverted this inhibition. Both tempol and DPI increased NO release in both NT and HT rings. The number of vascular smooth muscle cells (VSMC) and anti-α-actin positive areas were lower in HT than in NT rings, without variations in wall thickness or wall/lumen ratio. With regard to resting membrane potential, we found in HT rings that the depolarization induced by Ang II was abolished by tempol. These findings suggest that extraendothelial NO counterregulates Ang II contractility in IMA rings; however, its action could be altered in hypertensive situations even though the patients did not have associated risk factors. We suggest two mechanisms: increased oxidative stress and a decreased ability of nNOS in VSMC to produce NO.
Fil: Joo Turoni, Claudio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Marañón, Rodrigo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Proto, Victor. Centro Modelo de Cardiología; Argentina
Fil: Herrera, Ramon Nicasio Gerardo. Centro Modelo de Cardiología; Argentina
Fil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
Angiotensin Ii
Bypass Graft
Coronary Arteries
Endothelial Dysfunction
Hypertension
Nitric Oxide (No)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/62829
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/62829
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factorsJoo Turoni, Claudio MartínMarañón, Rodrigo OscarProto, VictorHerrera, Ramon Nicasio GerardoPeral, Maria de Los AngelesAngiotensin IiBypass GraftCoronary ArteriesEndothelial DysfunctionHypertensionNitric Oxide (No)https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We investigated the effects of extraendothelial nitric oxide (NO) on angiotensin II (Ang II) reactivity in internal mammary artery (IMA) rings, as well as the impact of hypertension without associated risk factors in this response. Vascular reactivity, NO levels, and resting membrane potentials were determined in hypertensive (HT) and normotensive (NT) IMA rings. Only rings with endothelial dysfunction were included. Ang II produced a dose-dependent contraction that was higher in HT rings. Response to Ang II was potentiated by Nω-nitro L-arginine methyl ester (L-NAME) in NT but not in HT rings. The antioxidant agents tempol and diphenyleneiodonium (DPI) reverted the hyperreactivity to Ang II in HT rings. Extraendothelial NO was present in both NT and HT rings. However, NT rings showed higher values. LNAME and S-methyl-L-thiocitrulline inhibited NO release in all cases. L-arginine reverted this inhibition. Both tempol and DPI increased NO release in both NT and HT rings. The number of vascular smooth muscle cells (VSMC) and anti-α-actin positive areas were lower in HT than in NT rings, without variations in wall thickness or wall/lumen ratio. With regard to resting membrane potential, we found in HT rings that the depolarization induced by Ang II was abolished by tempol. These findings suggest that extraendothelial NO counterregulates Ang II contractility in IMA rings; however, its action could be altered in hypertensive situations even though the patients did not have associated risk factors. We suggest two mechanisms: increased oxidative stress and a decreased ability of nNOS in VSMC to produce NO.Fil: Joo Turoni, Claudio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Marañón, Rodrigo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Proto, Victor. Centro Modelo de Cardiología; ArgentinaFil: Herrera, Ramon Nicasio Gerardo. Centro Modelo de Cardiología; ArgentinaFil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaTaylor & Francis2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62829Joo Turoni, Claudio Martín; Marañón, Rodrigo Oscar; Proto, Victor; Herrera, Ramon Nicasio Gerardo; Peral, Maria de Los Angeles; Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors; Taylor & Francis; Clinical and Experimental Hypertension; 33; 1; 1-2011; 27-331064-1963CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://informahealthcare.com/doi/abs/10.3109/10641963.2010.503297info:eu-repo/semantics/altIdentifier/doi/10.3109/10641963.2010.503297info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:12:56Zoai:ri.conicet.gov.ar:11336/62829instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:12:57.028CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors
title Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors
spellingShingle Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors
Joo Turoni, Claudio Martín
Angiotensin Ii
Bypass Graft
Coronary Arteries
Endothelial Dysfunction
Hypertension
Nitric Oxide (No)
title_short Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors
title_full Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors
title_fullStr Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors
title_full_unstemmed Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors
title_sort Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors
dc.creator.none.fl_str_mv Joo Turoni, Claudio Martín
Marañón, Rodrigo Oscar
Proto, Victor
Herrera, Ramon Nicasio Gerardo
Peral, Maria de Los Angeles
author Joo Turoni, Claudio Martín
author_facet Joo Turoni, Claudio Martín
Marañón, Rodrigo Oscar
Proto, Victor
Herrera, Ramon Nicasio Gerardo
Peral, Maria de Los Angeles
author_role author
author2 Marañón, Rodrigo Oscar
Proto, Victor
Herrera, Ramon Nicasio Gerardo
Peral, Maria de Los Angeles
author2_role author
author
author
author
dc.subject.none.fl_str_mv Angiotensin Ii
Bypass Graft
Coronary Arteries
Endothelial Dysfunction
Hypertension
Nitric Oxide (No)
topic Angiotensin Ii
Bypass Graft
Coronary Arteries
Endothelial Dysfunction
Hypertension
Nitric Oxide (No)
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We investigated the effects of extraendothelial nitric oxide (NO) on angiotensin II (Ang II) reactivity in internal mammary artery (IMA) rings, as well as the impact of hypertension without associated risk factors in this response. Vascular reactivity, NO levels, and resting membrane potentials were determined in hypertensive (HT) and normotensive (NT) IMA rings. Only rings with endothelial dysfunction were included. Ang II produced a dose-dependent contraction that was higher in HT rings. Response to Ang II was potentiated by Nω-nitro L-arginine methyl ester (L-NAME) in NT but not in HT rings. The antioxidant agents tempol and diphenyleneiodonium (DPI) reverted the hyperreactivity to Ang II in HT rings. Extraendothelial NO was present in both NT and HT rings. However, NT rings showed higher values. LNAME and S-methyl-L-thiocitrulline inhibited NO release in all cases. L-arginine reverted this inhibition. Both tempol and DPI increased NO release in both NT and HT rings. The number of vascular smooth muscle cells (VSMC) and anti-α-actin positive areas were lower in HT than in NT rings, without variations in wall thickness or wall/lumen ratio. With regard to resting membrane potential, we found in HT rings that the depolarization induced by Ang II was abolished by tempol. These findings suggest that extraendothelial NO counterregulates Ang II contractility in IMA rings; however, its action could be altered in hypertensive situations even though the patients did not have associated risk factors. We suggest two mechanisms: increased oxidative stress and a decreased ability of nNOS in VSMC to produce NO.
Fil: Joo Turoni, Claudio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Marañón, Rodrigo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Proto, Victor. Centro Modelo de Cardiología; Argentina
Fil: Herrera, Ramon Nicasio Gerardo. Centro Modelo de Cardiología; Argentina
Fil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description We investigated the effects of extraendothelial nitric oxide (NO) on angiotensin II (Ang II) reactivity in internal mammary artery (IMA) rings, as well as the impact of hypertension without associated risk factors in this response. Vascular reactivity, NO levels, and resting membrane potentials were determined in hypertensive (HT) and normotensive (NT) IMA rings. Only rings with endothelial dysfunction were included. Ang II produced a dose-dependent contraction that was higher in HT rings. Response to Ang II was potentiated by Nω-nitro L-arginine methyl ester (L-NAME) in NT but not in HT rings. The antioxidant agents tempol and diphenyleneiodonium (DPI) reverted the hyperreactivity to Ang II in HT rings. Extraendothelial NO was present in both NT and HT rings. However, NT rings showed higher values. LNAME and S-methyl-L-thiocitrulline inhibited NO release in all cases. L-arginine reverted this inhibition. Both tempol and DPI increased NO release in both NT and HT rings. The number of vascular smooth muscle cells (VSMC) and anti-α-actin positive areas were lower in HT than in NT rings, without variations in wall thickness or wall/lumen ratio. With regard to resting membrane potential, we found in HT rings that the depolarization induced by Ang II was abolished by tempol. These findings suggest that extraendothelial NO counterregulates Ang II contractility in IMA rings; however, its action could be altered in hypertensive situations even though the patients did not have associated risk factors. We suggest two mechanisms: increased oxidative stress and a decreased ability of nNOS in VSMC to produce NO.
publishDate 2011
dc.date.none.fl_str_mv 2011-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/62829
Joo Turoni, Claudio Martín; Marañón, Rodrigo Oscar; Proto, Victor; Herrera, Ramon Nicasio Gerardo; Peral, Maria de Los Angeles; Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors; Taylor & Francis; Clinical and Experimental Hypertension; 33; 1; 1-2011; 27-33
1064-1963
CONICET Digital
CONICET
url http://hdl.handle.net/11336/62829
identifier_str_mv Joo Turoni, Claudio Martín; Marañón, Rodrigo Oscar; Proto, Victor; Herrera, Ramon Nicasio Gerardo; Peral, Maria de Los Angeles; Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors; Taylor & Francis; Clinical and Experimental Hypertension; 33; 1; 1-2011; 27-33
1064-1963
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://informahealthcare.com/doi/abs/10.3109/10641963.2010.503297
info:eu-repo/semantics/altIdentifier/doi/10.3109/10641963.2010.503297
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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