Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors
- Autores
- Joo Turoni, Claudio Martín; Marañón, Rodrigo Oscar; Proto, Victor; Herrera, Ramon Nicasio Gerardo; Peral, Maria de Los Angeles
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We investigated the effects of extraendothelial nitric oxide (NO) on angiotensin II (Ang II) reactivity in internal mammary artery (IMA) rings, as well as the impact of hypertension without associated risk factors in this response. Vascular reactivity, NO levels, and resting membrane potentials were determined in hypertensive (HT) and normotensive (NT) IMA rings. Only rings with endothelial dysfunction were included. Ang II produced a dose-dependent contraction that was higher in HT rings. Response to Ang II was potentiated by Nω-nitro L-arginine methyl ester (L-NAME) in NT but not in HT rings. The antioxidant agents tempol and diphenyleneiodonium (DPI) reverted the hyperreactivity to Ang II in HT rings. Extraendothelial NO was present in both NT and HT rings. However, NT rings showed higher values. LNAME and S-methyl-L-thiocitrulline inhibited NO release in all cases. L-arginine reverted this inhibition. Both tempol and DPI increased NO release in both NT and HT rings. The number of vascular smooth muscle cells (VSMC) and anti-α-actin positive areas were lower in HT than in NT rings, without variations in wall thickness or wall/lumen ratio. With regard to resting membrane potential, we found in HT rings that the depolarization induced by Ang II was abolished by tempol. These findings suggest that extraendothelial NO counterregulates Ang II contractility in IMA rings; however, its action could be altered in hypertensive situations even though the patients did not have associated risk factors. We suggest two mechanisms: increased oxidative stress and a decreased ability of nNOS in VSMC to produce NO.
Fil: Joo Turoni, Claudio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Marañón, Rodrigo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Proto, Victor. Centro Modelo de Cardiología; Argentina
Fil: Herrera, Ramon Nicasio Gerardo. Centro Modelo de Cardiología; Argentina
Fil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina - Materia
-
Angiotensin Ii
Bypass Graft
Coronary Arteries
Endothelial Dysfunction
Hypertension
Nitric Oxide (No) - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/62829
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spelling |
Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factorsJoo Turoni, Claudio MartínMarañón, Rodrigo OscarProto, VictorHerrera, Ramon Nicasio GerardoPeral, Maria de Los AngelesAngiotensin IiBypass GraftCoronary ArteriesEndothelial DysfunctionHypertensionNitric Oxide (No)https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We investigated the effects of extraendothelial nitric oxide (NO) on angiotensin II (Ang II) reactivity in internal mammary artery (IMA) rings, as well as the impact of hypertension without associated risk factors in this response. Vascular reactivity, NO levels, and resting membrane potentials were determined in hypertensive (HT) and normotensive (NT) IMA rings. Only rings with endothelial dysfunction were included. Ang II produced a dose-dependent contraction that was higher in HT rings. Response to Ang II was potentiated by Nω-nitro L-arginine methyl ester (L-NAME) in NT but not in HT rings. The antioxidant agents tempol and diphenyleneiodonium (DPI) reverted the hyperreactivity to Ang II in HT rings. Extraendothelial NO was present in both NT and HT rings. However, NT rings showed higher values. LNAME and S-methyl-L-thiocitrulline inhibited NO release in all cases. L-arginine reverted this inhibition. Both tempol and DPI increased NO release in both NT and HT rings. The number of vascular smooth muscle cells (VSMC) and anti-α-actin positive areas were lower in HT than in NT rings, without variations in wall thickness or wall/lumen ratio. With regard to resting membrane potential, we found in HT rings that the depolarization induced by Ang II was abolished by tempol. These findings suggest that extraendothelial NO counterregulates Ang II contractility in IMA rings; however, its action could be altered in hypertensive situations even though the patients did not have associated risk factors. We suggest two mechanisms: increased oxidative stress and a decreased ability of nNOS in VSMC to produce NO.Fil: Joo Turoni, Claudio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Marañón, Rodrigo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Proto, Victor. Centro Modelo de Cardiología; ArgentinaFil: Herrera, Ramon Nicasio Gerardo. Centro Modelo de Cardiología; ArgentinaFil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaTaylor & Francis2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62829Joo Turoni, Claudio Martín; Marañón, Rodrigo Oscar; Proto, Victor; Herrera, Ramon Nicasio Gerardo; Peral, Maria de Los Angeles; Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors; Taylor & Francis; Clinical and Experimental Hypertension; 33; 1; 1-2011; 27-331064-1963CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://informahealthcare.com/doi/abs/10.3109/10641963.2010.503297info:eu-repo/semantics/altIdentifier/doi/10.3109/10641963.2010.503297info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:41Zoai:ri.conicet.gov.ar:11336/62829instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:42.264CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors |
title |
Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors |
spellingShingle |
Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors Joo Turoni, Claudio Martín Angiotensin Ii Bypass Graft Coronary Arteries Endothelial Dysfunction Hypertension Nitric Oxide (No) |
title_short |
Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors |
title_full |
Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors |
title_fullStr |
Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors |
title_full_unstemmed |
Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors |
title_sort |
Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors |
dc.creator.none.fl_str_mv |
Joo Turoni, Claudio Martín Marañón, Rodrigo Oscar Proto, Victor Herrera, Ramon Nicasio Gerardo Peral, Maria de Los Angeles |
author |
Joo Turoni, Claudio Martín |
author_facet |
Joo Turoni, Claudio Martín Marañón, Rodrigo Oscar Proto, Victor Herrera, Ramon Nicasio Gerardo Peral, Maria de Los Angeles |
author_role |
author |
author2 |
Marañón, Rodrigo Oscar Proto, Victor Herrera, Ramon Nicasio Gerardo Peral, Maria de Los Angeles |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Angiotensin Ii Bypass Graft Coronary Arteries Endothelial Dysfunction Hypertension Nitric Oxide (No) |
topic |
Angiotensin Ii Bypass Graft Coronary Arteries Endothelial Dysfunction Hypertension Nitric Oxide (No) |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
We investigated the effects of extraendothelial nitric oxide (NO) on angiotensin II (Ang II) reactivity in internal mammary artery (IMA) rings, as well as the impact of hypertension without associated risk factors in this response. Vascular reactivity, NO levels, and resting membrane potentials were determined in hypertensive (HT) and normotensive (NT) IMA rings. Only rings with endothelial dysfunction were included. Ang II produced a dose-dependent contraction that was higher in HT rings. Response to Ang II was potentiated by Nω-nitro L-arginine methyl ester (L-NAME) in NT but not in HT rings. The antioxidant agents tempol and diphenyleneiodonium (DPI) reverted the hyperreactivity to Ang II in HT rings. Extraendothelial NO was present in both NT and HT rings. However, NT rings showed higher values. LNAME and S-methyl-L-thiocitrulline inhibited NO release in all cases. L-arginine reverted this inhibition. Both tempol and DPI increased NO release in both NT and HT rings. The number of vascular smooth muscle cells (VSMC) and anti-α-actin positive areas were lower in HT than in NT rings, without variations in wall thickness or wall/lumen ratio. With regard to resting membrane potential, we found in HT rings that the depolarization induced by Ang II was abolished by tempol. These findings suggest that extraendothelial NO counterregulates Ang II contractility in IMA rings; however, its action could be altered in hypertensive situations even though the patients did not have associated risk factors. We suggest two mechanisms: increased oxidative stress and a decreased ability of nNOS in VSMC to produce NO. Fil: Joo Turoni, Claudio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Marañón, Rodrigo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Proto, Victor. Centro Modelo de Cardiología; Argentina Fil: Herrera, Ramon Nicasio Gerardo. Centro Modelo de Cardiología; Argentina Fil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina |
description |
We investigated the effects of extraendothelial nitric oxide (NO) on angiotensin II (Ang II) reactivity in internal mammary artery (IMA) rings, as well as the impact of hypertension without associated risk factors in this response. Vascular reactivity, NO levels, and resting membrane potentials were determined in hypertensive (HT) and normotensive (NT) IMA rings. Only rings with endothelial dysfunction were included. Ang II produced a dose-dependent contraction that was higher in HT rings. Response to Ang II was potentiated by Nω-nitro L-arginine methyl ester (L-NAME) in NT but not in HT rings. The antioxidant agents tempol and diphenyleneiodonium (DPI) reverted the hyperreactivity to Ang II in HT rings. Extraendothelial NO was present in both NT and HT rings. However, NT rings showed higher values. LNAME and S-methyl-L-thiocitrulline inhibited NO release in all cases. L-arginine reverted this inhibition. Both tempol and DPI increased NO release in both NT and HT rings. The number of vascular smooth muscle cells (VSMC) and anti-α-actin positive areas were lower in HT than in NT rings, without variations in wall thickness or wall/lumen ratio. With regard to resting membrane potential, we found in HT rings that the depolarization induced by Ang II was abolished by tempol. These findings suggest that extraendothelial NO counterregulates Ang II contractility in IMA rings; however, its action could be altered in hypertensive situations even though the patients did not have associated risk factors. We suggest two mechanisms: increased oxidative stress and a decreased ability of nNOS in VSMC to produce NO. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/62829 Joo Turoni, Claudio Martín; Marañón, Rodrigo Oscar; Proto, Victor; Herrera, Ramon Nicasio Gerardo; Peral, Maria de Los Angeles; Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors; Taylor & Francis; Clinical and Experimental Hypertension; 33; 1; 1-2011; 27-33 1064-1963 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/62829 |
identifier_str_mv |
Joo Turoni, Claudio Martín; Marañón, Rodrigo Oscar; Proto, Victor; Herrera, Ramon Nicasio Gerardo; Peral, Maria de Los Angeles; Nitric oxide modulates reactivity to angiotensin II in internal mammary arterial grafts in hytertensive patients without associated risk factors; Taylor & Francis; Clinical and Experimental Hypertension; 33; 1; 1-2011; 27-33 1064-1963 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://informahealthcare.com/doi/abs/10.3109/10641963.2010.503297 info:eu-repo/semantics/altIdentifier/doi/10.3109/10641963.2010.503297 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis |
publisher.none.fl_str_mv |
Taylor & Francis |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269653903081472 |
score |
13.13397 |