A novel cytokinin transporter is involved in local root signalling, modulating lateral root emergence

Autores
Tessi, Tomás María; Brumm, Sabine; Winklbauer, Eva; Greffen, Christopher; González, Claudio Alejandro; Maurino, Verónica; Harter, Klaus; Desimone, Marcelo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Aerial and underground organs of plants response to the environment modulating their architecture via tightly coordinated processes. Most of these processes are regulated by the morphogenic hormones auxins and cytokinins (CKs). Recently, hormone transport is being considered as a major modulator in hormone signa- lling. However, contrary to auxins, little is known about CK transport. In this work the Arabidopsis transporter AtAZG2 is studied. Although it was first described as a purine transporter, AtAZG2 is also able to take up CKs. Molecular evolution analysis revealed the presence of an AZG homolog in Charophytes (K. flaccidum) - the first known organism with complete CKs signalling pathway. Interestingly, molecular diversification of this transporter family occured in Tracheophytes, the first plants with complex roots. This is interesting regarding AtAZG2 expression domain, which is restricted to few cells surrounding the lateral root (LR) primordia. Furt- hermore, AtAZG2 KOs show lower LR density than Wt suggesting that AtAZG2 is part of a LR emergence re- gulatory pathway. To locally study the relevance of AtAZG2, the CK reporter TCSn:GFP was analysed in KOs. Quantification revealed weaker activity of the reporter in KOs compare to Wt, suggesting a direct link between AtAZG2 CK transport and LR emergence.
Fil: Tessi, Tomás María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Brumm, Sabine. Zentrum für Molekularbiologie der Pflanzen; Alemania
Fil: Winklbauer, Eva. Zentrum für Molekularbiologie der Pflanzen; Alemania
Fil: Greffen, Christopher. Zentrum für Molekularbiologie der Pflanzen; Alemania
Fil: González, Claudio Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina
Fil: Maurino, Verónica. Universitat zu Köln; Alemania
Fil: Harter, Klaus. Zentrum für Molekularbiologie der Pflanzen; Alemania
Fil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
XXXII Reunión Argentina; XVI Congreso Latinoamericano de Fisiología Vegetal
Córdoba
Argentina
Sociedad Argentina de Fisiología Vegetal
Materia
Transport
Cytokinins
Arabidopsis
Roots
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/178519
Acceder
id CONICETDig_19c527dd2ca4582d248dac7fa494f3d3
oai_identifier_str oai:ri.conicet.gov.ar:11336/178519
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A novel cytokinin transporter is involved in local root signalling, modulating lateral root emergenceUn nuevo transportador de citoquininas está involucrado en la señalización local radical, modulando la emergencia de raíces lateralesTessi, Tomás MaríaBrumm, SabineWinklbauer, EvaGreffen, ChristopherGonzález, Claudio AlejandroMaurino, VerónicaHarter, KlausDesimone, MarceloTransportCytokininsArabidopsisRootshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aerial and underground organs of plants response to the environment modulating their architecture via tightly coordinated processes. Most of these processes are regulated by the morphogenic hormones auxins and cytokinins (CKs). Recently, hormone transport is being considered as a major modulator in hormone signa- lling. However, contrary to auxins, little is known about CK transport. In this work the Arabidopsis transporter AtAZG2 is studied. Although it was first described as a purine transporter, AtAZG2 is also able to take up CKs. Molecular evolution analysis revealed the presence of an AZG homolog in Charophytes (K. flaccidum) - the first known organism with complete CKs signalling pathway. Interestingly, molecular diversification of this transporter family occured in Tracheophytes, the first plants with complex roots. This is interesting regarding AtAZG2 expression domain, which is restricted to few cells surrounding the lateral root (LR) primordia. Furt- hermore, AtAZG2 KOs show lower LR density than Wt suggesting that AtAZG2 is part of a LR emergence re- gulatory pathway. To locally study the relevance of AtAZG2, the CK reporter TCSn:GFP was analysed in KOs. Quantification revealed weaker activity of the reporter in KOs compare to Wt, suggesting a direct link between AtAZG2 CK transport and LR emergence.Fil: Tessi, Tomás María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Brumm, Sabine. Zentrum für Molekularbiologie der Pflanzen; AlemaniaFil: Winklbauer, Eva. Zentrum für Molekularbiologie der Pflanzen; AlemaniaFil: Greffen, Christopher. Zentrum für Molekularbiologie der Pflanzen; AlemaniaFil: González, Claudio Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Maurino, Verónica. Universitat zu Köln; AlemaniaFil: Harter, Klaus. Zentrum für Molekularbiologie der Pflanzen; AlemaniaFil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaXXXII Reunión Argentina; XVI Congreso Latinoamericano de Fisiología VegetalCórdobaArgentinaSociedad Argentina de Fisiología VegetalSociedad Argentina de Fisiología Vegetal2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/178519A novel cytokinin transporter is involved in local root signalling, modulating lateral root emergence; XXXII Reunión Argentina; XVI Congreso Latinoamericano de Fisiología Vegetal; Córdoba; Argentina; 2018; 122-123CONICET DigitalCONICETengInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:07:19Zoai:ri.conicet.gov.ar:11336/178519instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:07:19.904CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A novel cytokinin transporter is involved in local root signalling, modulating lateral root emergence
Un nuevo transportador de citoquininas está involucrado en la señalización local radical, modulando la emergencia de raíces laterales
title A novel cytokinin transporter is involved in local root signalling, modulating lateral root emergence
spellingShingle A novel cytokinin transporter is involved in local root signalling, modulating lateral root emergence
Tessi, Tomás María
Transport
Cytokinins
Arabidopsis
Roots
title_short A novel cytokinin transporter is involved in local root signalling, modulating lateral root emergence
title_full A novel cytokinin transporter is involved in local root signalling, modulating lateral root emergence
title_fullStr A novel cytokinin transporter is involved in local root signalling, modulating lateral root emergence
title_full_unstemmed A novel cytokinin transporter is involved in local root signalling, modulating lateral root emergence
title_sort A novel cytokinin transporter is involved in local root signalling, modulating lateral root emergence
dc.creator.none.fl_str_mv Tessi, Tomás María
Brumm, Sabine
Winklbauer, Eva
Greffen, Christopher
González, Claudio Alejandro
Maurino, Verónica
Harter, Klaus
Desimone, Marcelo
author Tessi, Tomás María
author_facet Tessi, Tomás María
Brumm, Sabine
Winklbauer, Eva
Greffen, Christopher
González, Claudio Alejandro
Maurino, Verónica
Harter, Klaus
Desimone, Marcelo
author_role author
author2 Brumm, Sabine
Winklbauer, Eva
Greffen, Christopher
González, Claudio Alejandro
Maurino, Verónica
Harter, Klaus
Desimone, Marcelo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Transport
Cytokinins
Arabidopsis
Roots
topic Transport
Cytokinins
Arabidopsis
Roots
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aerial and underground organs of plants response to the environment modulating their architecture via tightly coordinated processes. Most of these processes are regulated by the morphogenic hormones auxins and cytokinins (CKs). Recently, hormone transport is being considered as a major modulator in hormone signa- lling. However, contrary to auxins, little is known about CK transport. In this work the Arabidopsis transporter AtAZG2 is studied. Although it was first described as a purine transporter, AtAZG2 is also able to take up CKs. Molecular evolution analysis revealed the presence of an AZG homolog in Charophytes (K. flaccidum) - the first known organism with complete CKs signalling pathway. Interestingly, molecular diversification of this transporter family occured in Tracheophytes, the first plants with complex roots. This is interesting regarding AtAZG2 expression domain, which is restricted to few cells surrounding the lateral root (LR) primordia. Furt- hermore, AtAZG2 KOs show lower LR density than Wt suggesting that AtAZG2 is part of a LR emergence re- gulatory pathway. To locally study the relevance of AtAZG2, the CK reporter TCSn:GFP was analysed in KOs. Quantification revealed weaker activity of the reporter in KOs compare to Wt, suggesting a direct link between AtAZG2 CK transport and LR emergence.
Fil: Tessi, Tomás María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Brumm, Sabine. Zentrum für Molekularbiologie der Pflanzen; Alemania
Fil: Winklbauer, Eva. Zentrum für Molekularbiologie der Pflanzen; Alemania
Fil: Greffen, Christopher. Zentrum für Molekularbiologie der Pflanzen; Alemania
Fil: González, Claudio Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina
Fil: Maurino, Verónica. Universitat zu Köln; Alemania
Fil: Harter, Klaus. Zentrum für Molekularbiologie der Pflanzen; Alemania
Fil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
XXXII Reunión Argentina; XVI Congreso Latinoamericano de Fisiología Vegetal
Córdoba
Argentina
Sociedad Argentina de Fisiología Vegetal
description Aerial and underground organs of plants response to the environment modulating their architecture via tightly coordinated processes. Most of these processes are regulated by the morphogenic hormones auxins and cytokinins (CKs). Recently, hormone transport is being considered as a major modulator in hormone signa- lling. However, contrary to auxins, little is known about CK transport. In this work the Arabidopsis transporter AtAZG2 is studied. Although it was first described as a purine transporter, AtAZG2 is also able to take up CKs. Molecular evolution analysis revealed the presence of an AZG homolog in Charophytes (K. flaccidum) - the first known organism with complete CKs signalling pathway. Interestingly, molecular diversification of this transporter family occured in Tracheophytes, the first plants with complex roots. This is interesting regarding AtAZG2 expression domain, which is restricted to few cells surrounding the lateral root (LR) primordia. Furt- hermore, AtAZG2 KOs show lower LR density than Wt suggesting that AtAZG2 is part of a LR emergence re- gulatory pathway. To locally study the relevance of AtAZG2, the CK reporter TCSn:GFP was analysed in KOs. Quantification revealed weaker activity of the reporter in KOs compare to Wt, suggesting a direct link between AtAZG2 CK transport and LR emergence.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/178519
A novel cytokinin transporter is involved in local root signalling, modulating lateral root emergence; XXXII Reunión Argentina; XVI Congreso Latinoamericano de Fisiología Vegetal; Córdoba; Argentina; 2018; 122-123
CONICET Digital
CONICET
url http://hdl.handle.net/11336/178519
identifier_str_mv A novel cytokinin transporter is involved in local root signalling, modulating lateral root emergence; XXXII Reunión Argentina; XVI Congreso Latinoamericano de Fisiología Vegetal; Córdoba; Argentina; 2018; 122-123
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Fisiología Vegetal
publisher.none.fl_str_mv Sociedad Argentina de Fisiología Vegetal
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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