Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicu...

Autores
Mignolli, Francesco; Mariotti, Lorenzo; Picciarelli, Piero; Vidoz, María Laura
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aerial roots (aer) mutant of tomato is characterized by a profuse and precocious formation of adven-titious root primordia along the stem. We demonstrated that auxin is involved in the aer phenotype butruled out higher auxin sensitivity of mutant plants. Interestingly, polar auxin transport was altered in aer,as young seedlings showed a reduced response to an auxin transport inhibitor and higher expression ofauxin export carriers SlPIN1 and SlPIN3. An abrupt reduction in transcripts of auxin efflux and influx genesin older aer hypocotyls caused a marked deceleration of auxin transport in more mature tissues. Indeed,in 20 days old aer plants, the transport of labeled IAA was faster in apices than in hypocotyls, displayingan opposite trend in comparison to a wild type. In addition, auxin transport facilitators (SlPIN1, SlPIN4,SlLAX5) were more expressed in aer apices than in hypocotyls, suggesting that auxin moves faster fromthe upper to the lower part of the stem. Consequently, a significantly higher level of free and conjugatedIAA was found at the base of aer stems with respect to their apices. This auxin accumulation is likely thecause of the aer phenotype.
Fil: Mignolli, Francesco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Mariotti, Lorenzo. Università degli Studi di Pisa; Italia
Fil: Picciarelli, Piero. Università degli Studi di Pisa; Italia
Fil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Materia
Tomato
Aerial Roots Mutant
Adventitious Roots
Polar Auxin Transport
Iaa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29936
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/29936
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)Mignolli, FrancescoMariotti, LorenzoPicciarelli, PieroVidoz, María LauraTomatoAerial Roots MutantAdventitious RootsPolar Auxin TransportIaahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aerial roots (aer) mutant of tomato is characterized by a profuse and precocious formation of adven-titious root primordia along the stem. We demonstrated that auxin is involved in the aer phenotype butruled out higher auxin sensitivity of mutant plants. Interestingly, polar auxin transport was altered in aer,as young seedlings showed a reduced response to an auxin transport inhibitor and higher expression ofauxin export carriers SlPIN1 and SlPIN3. An abrupt reduction in transcripts of auxin efflux and influx genesin older aer hypocotyls caused a marked deceleration of auxin transport in more mature tissues. Indeed,in 20 days old aer plants, the transport of labeled IAA was faster in apices than in hypocotyls, displayingan opposite trend in comparison to a wild type. In addition, auxin transport facilitators (SlPIN1, SlPIN4,SlLAX5) were more expressed in aer apices than in hypocotyls, suggesting that auxin moves faster fromthe upper to the lower part of the stem. Consequently, a significantly higher level of free and conjugatedIAA was found at the base of aer stems with respect to their apices. This auxin accumulation is likely thecause of the aer phenotype.Fil: Mignolli, Francesco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Mariotti, Lorenzo. Università degli Studi di Pisa; ItaliaFil: Picciarelli, Piero. Università degli Studi di Pisa; ItaliaFil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaElsevier Gmbh2017-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29936Mignolli, Francesco; Mariotti, Lorenzo; Picciarelli, Piero; Vidoz, María Laura; Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.); Elsevier Gmbh; Journal of Plant Physiology; 213; 2-2017; 55-650176-1617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jplph.2017.02.010info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0176161717300627info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:29Zoai:ri.conicet.gov.ar:11336/29936instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:29.619CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)
title Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)
spellingShingle Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)
Mignolli, Francesco
Tomato
Aerial Roots Mutant
Adventitious Roots
Polar Auxin Transport
Iaa
title_short Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)
title_full Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)
title_fullStr Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)
title_full_unstemmed Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)
title_sort Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)
dc.creator.none.fl_str_mv Mignolli, Francesco
Mariotti, Lorenzo
Picciarelli, Piero
Vidoz, María Laura
author Mignolli, Francesco
author_facet Mignolli, Francesco
Mariotti, Lorenzo
Picciarelli, Piero
Vidoz, María Laura
author_role author
author2 Mariotti, Lorenzo
Picciarelli, Piero
Vidoz, María Laura
author2_role author
author
author
dc.subject.none.fl_str_mv Tomato
Aerial Roots Mutant
Adventitious Roots
Polar Auxin Transport
Iaa
topic Tomato
Aerial Roots Mutant
Adventitious Roots
Polar Auxin Transport
Iaa
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aerial roots (aer) mutant of tomato is characterized by a profuse and precocious formation of adven-titious root primordia along the stem. We demonstrated that auxin is involved in the aer phenotype butruled out higher auxin sensitivity of mutant plants. Interestingly, polar auxin transport was altered in aer,as young seedlings showed a reduced response to an auxin transport inhibitor and higher expression ofauxin export carriers SlPIN1 and SlPIN3. An abrupt reduction in transcripts of auxin efflux and influx genesin older aer hypocotyls caused a marked deceleration of auxin transport in more mature tissues. Indeed,in 20 days old aer plants, the transport of labeled IAA was faster in apices than in hypocotyls, displayingan opposite trend in comparison to a wild type. In addition, auxin transport facilitators (SlPIN1, SlPIN4,SlLAX5) were more expressed in aer apices than in hypocotyls, suggesting that auxin moves faster fromthe upper to the lower part of the stem. Consequently, a significantly higher level of free and conjugatedIAA was found at the base of aer stems with respect to their apices. This auxin accumulation is likely thecause of the aer phenotype.
Fil: Mignolli, Francesco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Mariotti, Lorenzo. Università degli Studi di Pisa; Italia
Fil: Picciarelli, Piero. Università degli Studi di Pisa; Italia
Fil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
description The aerial roots (aer) mutant of tomato is characterized by a profuse and precocious formation of adven-titious root primordia along the stem. We demonstrated that auxin is involved in the aer phenotype butruled out higher auxin sensitivity of mutant plants. Interestingly, polar auxin transport was altered in aer,as young seedlings showed a reduced response to an auxin transport inhibitor and higher expression ofauxin export carriers SlPIN1 and SlPIN3. An abrupt reduction in transcripts of auxin efflux and influx genesin older aer hypocotyls caused a marked deceleration of auxin transport in more mature tissues. Indeed,in 20 days old aer plants, the transport of labeled IAA was faster in apices than in hypocotyls, displayingan opposite trend in comparison to a wild type. In addition, auxin transport facilitators (SlPIN1, SlPIN4,SlLAX5) were more expressed in aer apices than in hypocotyls, suggesting that auxin moves faster fromthe upper to the lower part of the stem. Consequently, a significantly higher level of free and conjugatedIAA was found at the base of aer stems with respect to their apices. This auxin accumulation is likely thecause of the aer phenotype.
publishDate 2017
dc.date.none.fl_str_mv 2017-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29936
Mignolli, Francesco; Mariotti, Lorenzo; Picciarelli, Piero; Vidoz, María Laura; Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.); Elsevier Gmbh; Journal of Plant Physiology; 213; 2-2017; 55-65
0176-1617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29936
identifier_str_mv Mignolli, Francesco; Mariotti, Lorenzo; Picciarelli, Piero; Vidoz, María Laura; Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.); Elsevier Gmbh; Journal of Plant Physiology; 213; 2-2017; 55-65
0176-1617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jplph.2017.02.010
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0176161717300627
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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