AZG1 is a cytokinin transporter that interacts with auxin transporter PIN1 and regulates the root stress response

Autores
Tessi, Tomás María; Maurino, Veronica G.; Shahriari, Mojgan; Meissner, Esther; Novak, Ondrej; Pasternak, Taras; Schumacher, Benjamin S.; Ditengou, Franck; Li, Zenglin; Duerr, Jasmin; Flubacher, Noemi S.; Nautscher, Moritz; Williams, Alyssa; Kazimierczak, Zuzanna; Strnad, Miroslav; Thumfart, Jörg-Oliver; Palme, Klaus; Desimone, Marcelo; Teale, William D.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An environmentally responsive root system is crucial for plant growth and crop yield, especially in suboptimal soil conditions. This responsiveness enables the plant to exploit regions of high nutrient density while simultaneously minimizing abiotic stress. Despite the vital importance of root systems in regulating plant growth, significant gaps of knowledge exist in the mechanisms that regulate their architecture. Auxin defines both the frequency of lateral root (LR) initiation and the rate of LR outgrowth. Here, we describe a search for proteins that regulate root system architecture (RSA) by interacting directly with a key auxin transporter, PIN1. The native separation of Arabidopsis plasma membrane protein complexes identified several PIN1 co-purifying proteins. Among them, AZG1 was subsequently confirmed as a PIN1 interactor. Here, we show that, in Arabidopsis, AZG1 is a cytokinin (CK) import protein that co-localizes with and stabilizes PIN1, linking auxin and CK transport streams. AZG1 expression in LR primordia is sensitive to NaCl, and the frequency of LRs is AZG1-dependent under salt stress. This report therefore identifies a potential point for auxin:cytokinin crosstalk, which shapes RSA in response to NaCl.
Fil: Tessi, Tomás María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Maurino, Veronica G.. Molecular Plant Physiology, Institute Of Cellular And; Alemania
Fil: Shahriari, Mojgan. Albert Ludwigs University of Freiburg; Alemania
Fil: Meissner, Esther. Philipps-Universität Marburg; Alemania
Fil: Novak, Ondrej. Institute of Experimental Botany ASCR and Palacky; República Checa
Fil: Pasternak, Taras. Albert Ludwigs University of Freiburg; Alemania
Fil: Schumacher, Benjamin S.. Eberhard Karls Universität Tübingen; Alemania
Fil: Ditengou, Franck. Albert Ludwigs University of Freiburg; Alemania
Fil: Li, Zenglin. Albert Ludwigs University of Freiburg; Alemania
Fil: Duerr, Jasmin. Albert Ludwigs University of Freiburg; Alemania
Fil: Flubacher, Noemi S.. Albert Ludwigs University of Freiburg; Alemania
Fil: Nautscher, Moritz. Albert Ludwigs University of Freiburg; Alemania
Fil: Williams, Alyssa. Albert Ludwigs University of Freiburg; Alemania
Fil: Kazimierczak, Zuzanna. Albert Ludwigs University of Freiburg; Alemania
Fil: Strnad, Miroslav. Institute of Experimental Botany ASCR and Palacky; República Checa
Fil: Thumfart, Jörg-Oliver. Albert Ludwigs University of Freiburg; Alemania
Fil: Palme, Klaus. Universitat Bonn; Alemania
Fil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Teale, William D.. Albert Ludwigs University of Freiburg; Alemania
Materia
AUXIN
AUXIN TRANSPORT
CROSSTALK
CYTOKININ
CYTOKININ TRANSPORT
LATERAL ROOT
SALT STRESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/216638
Acceder
id CONICETDig_fffa656e358a6a5d157cafa0b8a79b9c
oai_identifier_str oai:ri.conicet.gov.ar:11336/216638
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling AZG1 is a cytokinin transporter that interacts with auxin transporter PIN1 and regulates the root stress responseTessi, Tomás MaríaMaurino, Veronica G.Shahriari, MojganMeissner, EstherNovak, OndrejPasternak, TarasSchumacher, Benjamin S.Ditengou, FranckLi, ZenglinDuerr, JasminFlubacher, Noemi S.Nautscher, MoritzWilliams, AlyssaKazimierczak, ZuzannaStrnad, MiroslavThumfart, Jörg-OliverPalme, KlausDesimone, MarceloTeale, William D.AUXINAUXIN TRANSPORTCROSSTALKCYTOKININCYTOKININ TRANSPORTLATERAL ROOTSALT STRESShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1An environmentally responsive root system is crucial for plant growth and crop yield, especially in suboptimal soil conditions. This responsiveness enables the plant to exploit regions of high nutrient density while simultaneously minimizing abiotic stress. Despite the vital importance of root systems in regulating plant growth, significant gaps of knowledge exist in the mechanisms that regulate their architecture. Auxin defines both the frequency of lateral root (LR) initiation and the rate of LR outgrowth. Here, we describe a search for proteins that regulate root system architecture (RSA) by interacting directly with a key auxin transporter, PIN1. The native separation of Arabidopsis plasma membrane protein complexes identified several PIN1 co-purifying proteins. Among them, AZG1 was subsequently confirmed as a PIN1 interactor. Here, we show that, in Arabidopsis, AZG1 is a cytokinin (CK) import protein that co-localizes with and stabilizes PIN1, linking auxin and CK transport streams. AZG1 expression in LR primordia is sensitive to NaCl, and the frequency of LRs is AZG1-dependent under salt stress. This report therefore identifies a potential point for auxin:cytokinin crosstalk, which shapes RSA in response to NaCl.Fil: Tessi, Tomás María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Maurino, Veronica G.. Molecular Plant Physiology, Institute Of Cellular And; AlemaniaFil: Shahriari, Mojgan. Albert Ludwigs University of Freiburg; AlemaniaFil: Meissner, Esther. Philipps-Universität Marburg; AlemaniaFil: Novak, Ondrej. Institute of Experimental Botany ASCR and Palacky; República ChecaFil: Pasternak, Taras. Albert Ludwigs University of Freiburg; AlemaniaFil: Schumacher, Benjamin S.. Eberhard Karls Universität Tübingen; AlemaniaFil: Ditengou, Franck. Albert Ludwigs University of Freiburg; AlemaniaFil: Li, Zenglin. Albert Ludwigs University of Freiburg; AlemaniaFil: Duerr, Jasmin. Albert Ludwigs University of Freiburg; AlemaniaFil: Flubacher, Noemi S.. Albert Ludwigs University of Freiburg; AlemaniaFil: Nautscher, Moritz. Albert Ludwigs University of Freiburg; AlemaniaFil: Williams, Alyssa. Albert Ludwigs University of Freiburg; AlemaniaFil: Kazimierczak, Zuzanna. Albert Ludwigs University of Freiburg; AlemaniaFil: Strnad, Miroslav. Institute of Experimental Botany ASCR and Palacky; República ChecaFil: Thumfart, Jörg-Oliver. Albert Ludwigs University of Freiburg; AlemaniaFil: Palme, Klaus. Universitat Bonn; AlemaniaFil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Teale, William D.. Albert Ludwigs University of Freiburg; AlemaniaWiley Blackwell Publishing, Inc2023-03-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/216638Tessi, Tomás María; Maurino, Veronica G.; Shahriari, Mojgan; Meissner, Esther; Novak, Ondrej; et al.; AZG1 is a cytokinin transporter that interacts with auxin transporter PIN1 and regulates the root stress response; Wiley Blackwell Publishing, Inc; New Phytologist; 238; 5; 14-3-2023; 1924-19410028-646X1469-8137CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/nph.18879info:eu-repo/semantics/altIdentifier/url/https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18879info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:20:05Zoai:ri.conicet.gov.ar:11336/216638instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:20:05.764CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv AZG1 is a cytokinin transporter that interacts with auxin transporter PIN1 and regulates the root stress response
title AZG1 is a cytokinin transporter that interacts with auxin transporter PIN1 and regulates the root stress response
spellingShingle AZG1 is a cytokinin transporter that interacts with auxin transporter PIN1 and regulates the root stress response
Tessi, Tomás María
AUXIN
AUXIN TRANSPORT
CROSSTALK
CYTOKININ
CYTOKININ TRANSPORT
LATERAL ROOT
SALT STRESS
title_short AZG1 is a cytokinin transporter that interacts with auxin transporter PIN1 and regulates the root stress response
title_full AZG1 is a cytokinin transporter that interacts with auxin transporter PIN1 and regulates the root stress response
title_fullStr AZG1 is a cytokinin transporter that interacts with auxin transporter PIN1 and regulates the root stress response
title_full_unstemmed AZG1 is a cytokinin transporter that interacts with auxin transporter PIN1 and regulates the root stress response
title_sort AZG1 is a cytokinin transporter that interacts with auxin transporter PIN1 and regulates the root stress response
dc.creator.none.fl_str_mv Tessi, Tomás María
Maurino, Veronica G.
Shahriari, Mojgan
Meissner, Esther
Novak, Ondrej
Pasternak, Taras
Schumacher, Benjamin S.
Ditengou, Franck
Li, Zenglin
Duerr, Jasmin
Flubacher, Noemi S.
Nautscher, Moritz
Williams, Alyssa
Kazimierczak, Zuzanna
Strnad, Miroslav
Thumfart, Jörg-Oliver
Palme, Klaus
Desimone, Marcelo
Teale, William D.
author Tessi, Tomás María
author_facet Tessi, Tomás María
Maurino, Veronica G.
Shahriari, Mojgan
Meissner, Esther
Novak, Ondrej
Pasternak, Taras
Schumacher, Benjamin S.
Ditengou, Franck
Li, Zenglin
Duerr, Jasmin
Flubacher, Noemi S.
Nautscher, Moritz
Williams, Alyssa
Kazimierczak, Zuzanna
Strnad, Miroslav
Thumfart, Jörg-Oliver
Palme, Klaus
Desimone, Marcelo
Teale, William D.
author_role author
author2 Maurino, Veronica G.
Shahriari, Mojgan
Meissner, Esther
Novak, Ondrej
Pasternak, Taras
Schumacher, Benjamin S.
Ditengou, Franck
Li, Zenglin
Duerr, Jasmin
Flubacher, Noemi S.
Nautscher, Moritz
Williams, Alyssa
Kazimierczak, Zuzanna
Strnad, Miroslav
Thumfart, Jörg-Oliver
Palme, Klaus
Desimone, Marcelo
Teale, William D.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AUXIN
AUXIN TRANSPORT
CROSSTALK
CYTOKININ
CYTOKININ TRANSPORT
LATERAL ROOT
SALT STRESS
topic AUXIN
AUXIN TRANSPORT
CROSSTALK
CYTOKININ
CYTOKININ TRANSPORT
LATERAL ROOT
SALT STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An environmentally responsive root system is crucial for plant growth and crop yield, especially in suboptimal soil conditions. This responsiveness enables the plant to exploit regions of high nutrient density while simultaneously minimizing abiotic stress. Despite the vital importance of root systems in regulating plant growth, significant gaps of knowledge exist in the mechanisms that regulate their architecture. Auxin defines both the frequency of lateral root (LR) initiation and the rate of LR outgrowth. Here, we describe a search for proteins that regulate root system architecture (RSA) by interacting directly with a key auxin transporter, PIN1. The native separation of Arabidopsis plasma membrane protein complexes identified several PIN1 co-purifying proteins. Among them, AZG1 was subsequently confirmed as a PIN1 interactor. Here, we show that, in Arabidopsis, AZG1 is a cytokinin (CK) import protein that co-localizes with and stabilizes PIN1, linking auxin and CK transport streams. AZG1 expression in LR primordia is sensitive to NaCl, and the frequency of LRs is AZG1-dependent under salt stress. This report therefore identifies a potential point for auxin:cytokinin crosstalk, which shapes RSA in response to NaCl.
Fil: Tessi, Tomás María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Maurino, Veronica G.. Molecular Plant Physiology, Institute Of Cellular And; Alemania
Fil: Shahriari, Mojgan. Albert Ludwigs University of Freiburg; Alemania
Fil: Meissner, Esther. Philipps-Universität Marburg; Alemania
Fil: Novak, Ondrej. Institute of Experimental Botany ASCR and Palacky; República Checa
Fil: Pasternak, Taras. Albert Ludwigs University of Freiburg; Alemania
Fil: Schumacher, Benjamin S.. Eberhard Karls Universität Tübingen; Alemania
Fil: Ditengou, Franck. Albert Ludwigs University of Freiburg; Alemania
Fil: Li, Zenglin. Albert Ludwigs University of Freiburg; Alemania
Fil: Duerr, Jasmin. Albert Ludwigs University of Freiburg; Alemania
Fil: Flubacher, Noemi S.. Albert Ludwigs University of Freiburg; Alemania
Fil: Nautscher, Moritz. Albert Ludwigs University of Freiburg; Alemania
Fil: Williams, Alyssa. Albert Ludwigs University of Freiburg; Alemania
Fil: Kazimierczak, Zuzanna. Albert Ludwigs University of Freiburg; Alemania
Fil: Strnad, Miroslav. Institute of Experimental Botany ASCR and Palacky; República Checa
Fil: Thumfart, Jörg-Oliver. Albert Ludwigs University of Freiburg; Alemania
Fil: Palme, Klaus. Universitat Bonn; Alemania
Fil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Teale, William D.. Albert Ludwigs University of Freiburg; Alemania
description An environmentally responsive root system is crucial for plant growth and crop yield, especially in suboptimal soil conditions. This responsiveness enables the plant to exploit regions of high nutrient density while simultaneously minimizing abiotic stress. Despite the vital importance of root systems in regulating plant growth, significant gaps of knowledge exist in the mechanisms that regulate their architecture. Auxin defines both the frequency of lateral root (LR) initiation and the rate of LR outgrowth. Here, we describe a search for proteins that regulate root system architecture (RSA) by interacting directly with a key auxin transporter, PIN1. The native separation of Arabidopsis plasma membrane protein complexes identified several PIN1 co-purifying proteins. Among them, AZG1 was subsequently confirmed as a PIN1 interactor. Here, we show that, in Arabidopsis, AZG1 is a cytokinin (CK) import protein that co-localizes with and stabilizes PIN1, linking auxin and CK transport streams. AZG1 expression in LR primordia is sensitive to NaCl, and the frequency of LRs is AZG1-dependent under salt stress. This report therefore identifies a potential point for auxin:cytokinin crosstalk, which shapes RSA in response to NaCl.
publishDate 2023
dc.date.none.fl_str_mv 2023-03-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/216638
Tessi, Tomás María; Maurino, Veronica G.; Shahriari, Mojgan; Meissner, Esther; Novak, Ondrej; et al.; AZG1 is a cytokinin transporter that interacts with auxin transporter PIN1 and regulates the root stress response; Wiley Blackwell Publishing, Inc; New Phytologist; 238; 5; 14-3-2023; 1924-1941
0028-646X
1469-8137
CONICET Digital
CONICET
url http://hdl.handle.net/11336/216638
identifier_str_mv Tessi, Tomás María; Maurino, Veronica G.; Shahriari, Mojgan; Meissner, Esther; Novak, Ondrej; et al.; AZG1 is a cytokinin transporter that interacts with auxin transporter PIN1 and regulates the root stress response; Wiley Blackwell Publishing, Inc; New Phytologist; 238; 5; 14-3-2023; 1924-1941
0028-646X
1469-8137
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.18879
info:eu-repo/semantics/altIdentifier/url/https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18879
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083349735538688
score 13.22299

Publicaciones similares