Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergence

Autores
Tessi, Tomás María; Brumm, Sabine; Winklbauer, Eva; Schumacher, Benjamín; Pettinari, Georgina Lucía; Lescano López, Ignacio; González, Claudio Alejandro; Wanke, Dierk; Maurino, Verónica; Harter, Klaus; Desimone, Marcelo
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cytokinin and auxin are key regulators of plant growth and development. During the last decade transport mechanisms have turned out to be the key for the control of local and long-distance hormone distributions. In contrast with auxin, cytokinin transport is poorly understood. Here, we show that Arabidopsis thaliana AZG2, a member of the AZG purine transporter family, acts as cytokinin transporter involved in root system architecture determination. Even though purines are substrates for both AZG1 and AZG2, we found distinct transport mechanisms. The expression of AZG2 is restricted to a small group of cells surrounding the lateral root (LR) primordia and induced by auxins. Compared to the wild-type (WT), mutants carrying loss-of-function alleles of AZG2 have higher LR density, suggesting that AZG2 is part of a regulatory pathway in LR emergence. Moreover, azg2 is partially insensitive to exogenous cytokinin, which is consistent with the observation that the cytokinin reporter TCSnpro:GFP showed lower fluorescence signal in the roots of azg2 compared to the WT. These results indicate a defective cytokinin signalling pathway in the region of LR primordia. The integration of AZG2 subcellular localization and cytokinin transport capacity data allowed us to propose a local cytokinin : auxin signalling model for the regulation of LR emergence.
Fil: Tessi, Tomás María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Brumm, Sabine. Eberhard Karls Universität Tübingen; Alemania
Fil: Winklbauer, Eva. Eberhard Karls Universität Tübingen; Alemania
Fil: Schumacher, Benjamín. Eberhard Karls Universität Tübingen; Alemania
Fil: Pettinari, Georgina Lucía. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Estudios Agropecuarios; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina
Fil: Lescano López, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: González, Claudio Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina
Fil: Wanke, Dierk. Eberhard Karls Universität Tübingen; Alemania
Fil: Maurino, Verónica. Universitat Bonn; Alemania
Fil: Harter, Klaus. Eberhard Karls Universität Tübingen; Alemania
Fil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina
Materia
AUXIN
AZG
CYTOKININ TRANSPORT
DEVELOPMENT
LATERAL ROOT
LATERAL ROOT OVERLYING TISSUES
LATERAL ROOT PRIMORDIA
PHYTOHORMONE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/146940
Acceder
id CONICETDig_1723f313a34c85f1f04a12266b5874f6
oai_identifier_str oai:ri.conicet.gov.ar:11336/146940
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergenceTessi, Tomás MaríaBrumm, SabineWinklbauer, EvaSchumacher, BenjamínPettinari, Georgina LucíaLescano López, IgnacioGonzález, Claudio AlejandroWanke, DierkMaurino, VerónicaHarter, KlausDesimone, MarceloAUXINAZGCYTOKININ TRANSPORTDEVELOPMENTLATERAL ROOTLATERAL ROOT OVERLYING TISSUESLATERAL ROOT PRIMORDIAPHYTOHORMONEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cytokinin and auxin are key regulators of plant growth and development. During the last decade transport mechanisms have turned out to be the key for the control of local and long-distance hormone distributions. In contrast with auxin, cytokinin transport is poorly understood. Here, we show that Arabidopsis thaliana AZG2, a member of the AZG purine transporter family, acts as cytokinin transporter involved in root system architecture determination. Even though purines are substrates for both AZG1 and AZG2, we found distinct transport mechanisms. The expression of AZG2 is restricted to a small group of cells surrounding the lateral root (LR) primordia and induced by auxins. Compared to the wild-type (WT), mutants carrying loss-of-function alleles of AZG2 have higher LR density, suggesting that AZG2 is part of a regulatory pathway in LR emergence. Moreover, azg2 is partially insensitive to exogenous cytokinin, which is consistent with the observation that the cytokinin reporter TCSnpro:GFP showed lower fluorescence signal in the roots of azg2 compared to the WT. These results indicate a defective cytokinin signalling pathway in the region of LR primordia. The integration of AZG2 subcellular localization and cytokinin transport capacity data allowed us to propose a local cytokinin : auxin signalling model for the regulation of LR emergence.Fil: Tessi, Tomás María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Brumm, Sabine. Eberhard Karls Universität Tübingen; AlemaniaFil: Winklbauer, Eva. Eberhard Karls Universität Tübingen; AlemaniaFil: Schumacher, Benjamín. Eberhard Karls Universität Tübingen; AlemaniaFil: Pettinari, Georgina Lucía. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Estudios Agropecuarios; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; ArgentinaFil: Lescano López, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: González, Claudio Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; ArgentinaFil: Wanke, Dierk. Eberhard Karls Universität Tübingen; AlemaniaFil: Maurino, Verónica. Universitat Bonn; AlemaniaFil: Harter, Klaus. Eberhard Karls Universität Tübingen; AlemaniaFil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; ArgentinaWiley Blackwell Publishing, Inc2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/146940Tessi, Tomás María; Brumm, Sabine; Winklbauer, Eva; Schumacher, Benjamín; Pettinari, Georgina Lucía; et al.; Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergence; Wiley Blackwell Publishing, Inc; New Phytologist; 229; 2; 1-2021; 979-9930028-646X1469-8137CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2020.01.31.927970v1info:eu-repo/semantics/altIdentifier/url/https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.16943info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.16943info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:07:45Zoai:ri.conicet.gov.ar:11336/146940instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:07:45.799CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergence
title Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergence
spellingShingle Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergence
Tessi, Tomás María
AUXIN
AZG
CYTOKININ TRANSPORT
DEVELOPMENT
LATERAL ROOT
LATERAL ROOT OVERLYING TISSUES
LATERAL ROOT PRIMORDIA
PHYTOHORMONE
title_short Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergence
title_full Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergence
title_fullStr Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergence
title_full_unstemmed Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergence
title_sort Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergence
dc.creator.none.fl_str_mv Tessi, Tomás María
Brumm, Sabine
Winklbauer, Eva
Schumacher, Benjamín
Pettinari, Georgina Lucía
Lescano López, Ignacio
González, Claudio Alejandro
Wanke, Dierk
Maurino, Verónica
Harter, Klaus
Desimone, Marcelo
author Tessi, Tomás María
author_facet Tessi, Tomás María
Brumm, Sabine
Winklbauer, Eva
Schumacher, Benjamín
Pettinari, Georgina Lucía
Lescano López, Ignacio
González, Claudio Alejandro
Wanke, Dierk
Maurino, Verónica
Harter, Klaus
Desimone, Marcelo
author_role author
author2 Brumm, Sabine
Winklbauer, Eva
Schumacher, Benjamín
Pettinari, Georgina Lucía
Lescano López, Ignacio
González, Claudio Alejandro
Wanke, Dierk
Maurino, Verónica
Harter, Klaus
Desimone, Marcelo
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AUXIN
AZG
CYTOKININ TRANSPORT
DEVELOPMENT
LATERAL ROOT
LATERAL ROOT OVERLYING TISSUES
LATERAL ROOT PRIMORDIA
PHYTOHORMONE
topic AUXIN
AZG
CYTOKININ TRANSPORT
DEVELOPMENT
LATERAL ROOT
LATERAL ROOT OVERLYING TISSUES
LATERAL ROOT PRIMORDIA
PHYTOHORMONE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cytokinin and auxin are key regulators of plant growth and development. During the last decade transport mechanisms have turned out to be the key for the control of local and long-distance hormone distributions. In contrast with auxin, cytokinin transport is poorly understood. Here, we show that Arabidopsis thaliana AZG2, a member of the AZG purine transporter family, acts as cytokinin transporter involved in root system architecture determination. Even though purines are substrates for both AZG1 and AZG2, we found distinct transport mechanisms. The expression of AZG2 is restricted to a small group of cells surrounding the lateral root (LR) primordia and induced by auxins. Compared to the wild-type (WT), mutants carrying loss-of-function alleles of AZG2 have higher LR density, suggesting that AZG2 is part of a regulatory pathway in LR emergence. Moreover, azg2 is partially insensitive to exogenous cytokinin, which is consistent with the observation that the cytokinin reporter TCSnpro:GFP showed lower fluorescence signal in the roots of azg2 compared to the WT. These results indicate a defective cytokinin signalling pathway in the region of LR primordia. The integration of AZG2 subcellular localization and cytokinin transport capacity data allowed us to propose a local cytokinin : auxin signalling model for the regulation of LR emergence.
Fil: Tessi, Tomás María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Brumm, Sabine. Eberhard Karls Universität Tübingen; Alemania
Fil: Winklbauer, Eva. Eberhard Karls Universität Tübingen; Alemania
Fil: Schumacher, Benjamín. Eberhard Karls Universität Tübingen; Alemania
Fil: Pettinari, Georgina Lucía. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Estudios Agropecuarios; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina
Fil: Lescano López, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: González, Claudio Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina
Fil: Wanke, Dierk. Eberhard Karls Universität Tübingen; Alemania
Fil: Maurino, Verónica. Universitat Bonn; Alemania
Fil: Harter, Klaus. Eberhard Karls Universität Tübingen; Alemania
Fil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina
description Cytokinin and auxin are key regulators of plant growth and development. During the last decade transport mechanisms have turned out to be the key for the control of local and long-distance hormone distributions. In contrast with auxin, cytokinin transport is poorly understood. Here, we show that Arabidopsis thaliana AZG2, a member of the AZG purine transporter family, acts as cytokinin transporter involved in root system architecture determination. Even though purines are substrates for both AZG1 and AZG2, we found distinct transport mechanisms. The expression of AZG2 is restricted to a small group of cells surrounding the lateral root (LR) primordia and induced by auxins. Compared to the wild-type (WT), mutants carrying loss-of-function alleles of AZG2 have higher LR density, suggesting that AZG2 is part of a regulatory pathway in LR emergence. Moreover, azg2 is partially insensitive to exogenous cytokinin, which is consistent with the observation that the cytokinin reporter TCSnpro:GFP showed lower fluorescence signal in the roots of azg2 compared to the WT. These results indicate a defective cytokinin signalling pathway in the region of LR primordia. The integration of AZG2 subcellular localization and cytokinin transport capacity data allowed us to propose a local cytokinin : auxin signalling model for the regulation of LR emergence.
publishDate 2021
dc.date.none.fl_str_mv 2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/146940
Tessi, Tomás María; Brumm, Sabine; Winklbauer, Eva; Schumacher, Benjamín; Pettinari, Georgina Lucía; et al.; Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergence; Wiley Blackwell Publishing, Inc; New Phytologist; 229; 2; 1-2021; 979-993
0028-646X
1469-8137
CONICET Digital
CONICET
url http://hdl.handle.net/11336/146940
identifier_str_mv Tessi, Tomás María; Brumm, Sabine; Winklbauer, Eva; Schumacher, Benjamín; Pettinari, Georgina Lucía; et al.; Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergence; Wiley Blackwell Publishing, Inc; New Phytologist; 229; 2; 1-2021; 979-993
0028-646X
1469-8137
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2020.01.31.927970v1
info:eu-repo/semantics/altIdentifier/url/https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.16943
info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.16943
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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