Cytokinin transporter azg2 modulates cell wall remodeling during lateral root emergence in Arabidopsis thaliana

Autores
Pettinari, Georgina Lucía; Tessi, Tomás María; González, Claudio Alejandro; Desimone, Marcelo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Tissue remodeling is essential for lateral root (LR) emergence. Many studies have focused on the role of auxin in this process but little is known about cytokinin (CK) relevance. AZG2 is an auxin regulated CK transporter, involved in negative regulation of LR development in Arabidopsis. AZG2 is expressed in a small group of cortical and epidermal cells (OLT) surrounding lateral root primordia (LRP), resembling some cell wall remodeling enzymes (CWREs) involved in lateral root emergence (LRE). Here, we study LRE and cell wall remodeling (CWR) in Arabidopsis roots of WT and azg2-1 genotypes through two independent experiments. LRP/LR index was calculated and values were 2,93 for azg2-1 and 3,48 for WT roots, confirming that AZG2 has a regulatory effect on LRE. Additionally, a lower propidium iodide fluorescence intensity was detected in the OLTs of azg2 seedlings compared to WT, suggesting an increased CWR activity in the mutant genotype. Taken together, these results indicate that CK transport mediated by AZG2 could modulate CWR during LRE. In order to get further insight, we are currently working on pCWRE:GUS:GFP/WT and pCWRE:GUS:GFP/azg2 reporter lines to study CK effect on the expression of two different CWREs.
Fil: Pettinari, Georgina Lucía. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tessi, Tomás María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: González, Claudio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
XXXII Reunión argentina XVI Congreso Latinoamericano de Fisiología Vegetal
Córdoba
Argentina
Sociedad Argentina de Fisiología Vegetal
Materia
Lateral root
Cell wall Remodeling
AZG2
Arabidopsis thaliana
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/131502
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Cytokinin transporter azg2 modulates cell wall remodeling during lateral root emergence in Arabidopsis thalianaPettinari, Georgina LucíaTessi, Tomás MaríaGonzález, Claudio AlejandroDesimone, MarceloLateral rootCell wall RemodelingAZG2Arabidopsis thalianahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Tissue remodeling is essential for lateral root (LR) emergence. Many studies have focused on the role of auxin in this process but little is known about cytokinin (CK) relevance. AZG2 is an auxin regulated CK transporter, involved in negative regulation of LR development in Arabidopsis. AZG2 is expressed in a small group of cortical and epidermal cells (OLT) surrounding lateral root primordia (LRP), resembling some cell wall remodeling enzymes (CWREs) involved in lateral root emergence (LRE). Here, we study LRE and cell wall remodeling (CWR) in Arabidopsis roots of WT and azg2-1 genotypes through two independent experiments. LRP/LR index was calculated and values were 2,93 for azg2-1 and 3,48 for WT roots, confirming that AZG2 has a regulatory effect on LRE. Additionally, a lower propidium iodide fluorescence intensity was detected in the OLTs of azg2 seedlings compared to WT, suggesting an increased CWR activity in the mutant genotype. Taken together, these results indicate that CK transport mediated by AZG2 could modulate CWR during LRE. In order to get further insight, we are currently working on pCWRE:GUS:GFP/WT and pCWRE:GUS:GFP/azg2 reporter lines to study CK effect on the expression of two different CWREs.Fil: Pettinari, Georgina Lucía. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tessi, Tomás María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: González, Claudio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaXXXII Reunión argentina XVI Congreso Latinoamericano de Fisiología VegetalCórdobaArgentinaSociedad Argentina de Fisiología VegetalSociedad Argentina de Fisiología Vegetal2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/131502Cytokinin transporter azg2 modulates cell wall remodeling during lateral root emergence in Arabidopsis thaliana; XXXII Reunión argentina XVI Congreso Latinoamericano de Fisiología Vegetal; Córdoba; Argentina; 2019; 63-63CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.fisiologiavegetal.org/vermas-noticias.php?n=255Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:03Zoai:ri.conicet.gov.ar:11336/131502instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:03.964CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cytokinin transporter azg2 modulates cell wall remodeling during lateral root emergence in Arabidopsis thaliana
title Cytokinin transporter azg2 modulates cell wall remodeling during lateral root emergence in Arabidopsis thaliana
spellingShingle Cytokinin transporter azg2 modulates cell wall remodeling during lateral root emergence in Arabidopsis thaliana
Pettinari, Georgina Lucía
Lateral root
Cell wall Remodeling
AZG2
Arabidopsis thaliana
title_short Cytokinin transporter azg2 modulates cell wall remodeling during lateral root emergence in Arabidopsis thaliana
title_full Cytokinin transporter azg2 modulates cell wall remodeling during lateral root emergence in Arabidopsis thaliana
title_fullStr Cytokinin transporter azg2 modulates cell wall remodeling during lateral root emergence in Arabidopsis thaliana
title_full_unstemmed Cytokinin transporter azg2 modulates cell wall remodeling during lateral root emergence in Arabidopsis thaliana
title_sort Cytokinin transporter azg2 modulates cell wall remodeling during lateral root emergence in Arabidopsis thaliana
dc.creator.none.fl_str_mv Pettinari, Georgina Lucía
Tessi, Tomás María
González, Claudio Alejandro
Desimone, Marcelo
author Pettinari, Georgina Lucía
author_facet Pettinari, Georgina Lucía
Tessi, Tomás María
González, Claudio Alejandro
Desimone, Marcelo
author_role author
author2 Tessi, Tomás María
González, Claudio Alejandro
Desimone, Marcelo
author2_role author
author
author
dc.subject.none.fl_str_mv Lateral root
Cell wall Remodeling
AZG2
Arabidopsis thaliana
topic Lateral root
Cell wall Remodeling
AZG2
Arabidopsis thaliana
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Tissue remodeling is essential for lateral root (LR) emergence. Many studies have focused on the role of auxin in this process but little is known about cytokinin (CK) relevance. AZG2 is an auxin regulated CK transporter, involved in negative regulation of LR development in Arabidopsis. AZG2 is expressed in a small group of cortical and epidermal cells (OLT) surrounding lateral root primordia (LRP), resembling some cell wall remodeling enzymes (CWREs) involved in lateral root emergence (LRE). Here, we study LRE and cell wall remodeling (CWR) in Arabidopsis roots of WT and azg2-1 genotypes through two independent experiments. LRP/LR index was calculated and values were 2,93 for azg2-1 and 3,48 for WT roots, confirming that AZG2 has a regulatory effect on LRE. Additionally, a lower propidium iodide fluorescence intensity was detected in the OLTs of azg2 seedlings compared to WT, suggesting an increased CWR activity in the mutant genotype. Taken together, these results indicate that CK transport mediated by AZG2 could modulate CWR during LRE. In order to get further insight, we are currently working on pCWRE:GUS:GFP/WT and pCWRE:GUS:GFP/azg2 reporter lines to study CK effect on the expression of two different CWREs.
Fil: Pettinari, Georgina Lucía. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tessi, Tomás María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: González, Claudio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
XXXII Reunión argentina XVI Congreso Latinoamericano de Fisiología Vegetal
Córdoba
Argentina
Sociedad Argentina de Fisiología Vegetal
description Tissue remodeling is essential for lateral root (LR) emergence. Many studies have focused on the role of auxin in this process but little is known about cytokinin (CK) relevance. AZG2 is an auxin regulated CK transporter, involved in negative regulation of LR development in Arabidopsis. AZG2 is expressed in a small group of cortical and epidermal cells (OLT) surrounding lateral root primordia (LRP), resembling some cell wall remodeling enzymes (CWREs) involved in lateral root emergence (LRE). Here, we study LRE and cell wall remodeling (CWR) in Arabidopsis roots of WT and azg2-1 genotypes through two independent experiments. LRP/LR index was calculated and values were 2,93 for azg2-1 and 3,48 for WT roots, confirming that AZG2 has a regulatory effect on LRE. Additionally, a lower propidium iodide fluorescence intensity was detected in the OLTs of azg2 seedlings compared to WT, suggesting an increased CWR activity in the mutant genotype. Taken together, these results indicate that CK transport mediated by AZG2 could modulate CWR during LRE. In order to get further insight, we are currently working on pCWRE:GUS:GFP/WT and pCWRE:GUS:GFP/azg2 reporter lines to study CK effect on the expression of two different CWREs.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131502
Cytokinin transporter azg2 modulates cell wall remodeling during lateral root emergence in Arabidopsis thaliana; XXXII Reunión argentina XVI Congreso Latinoamericano de Fisiología Vegetal; Córdoba; Argentina; 2019; 63-63
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131502
identifier_str_mv Cytokinin transporter azg2 modulates cell wall remodeling during lateral root emergence in Arabidopsis thaliana; XXXII Reunión argentina XVI Congreso Latinoamericano de Fisiología Vegetal; Córdoba; Argentina; 2019; 63-63
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.fisiologiavegetal.org/vermas-noticias.php?n=255
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/vnd.openxmlformats-officedocument.wordprocessingml.document
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Fisiología Vegetal
publisher.none.fl_str_mv Sociedad Argentina de Fisiología Vegetal
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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