An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus
- Autores
- Ribeiro, José M. C.; Genta, Fernando A.; Sorgine, Marcos H. F.; Mesquita, Rafael D.; Paiva Silva, Gabriela O.; Majerowicz, David; Medeiros, Marcelo; Koerich, Leonardo; Terra, Walter R.; Ferreira, Clelia; Pimentel, Andre C.; Bisch, Paulo M.; Leite, Daniel C.; Diniz, Michelle M. P.; Da Silva, Manuela L.; Araujo, Ricardo N.; Gandara, Ana Caroline P.; Brosson, Sebastien; Salmon, Didier; Bousbata, Sabrina; Gonzalez Caballero, Natalia; Alves Bezerra, Michele; Katia C. Gondim; Silva Neto, Mario Alberto C.; Atella, Georgia C.; Araujo, Helena; Dias, Felipe A.; Polycarpo, Carla; Vionette Amaral, Raquel J.; Fampa, Patricia; Melo, Ana Claudia A.; Tanaka, Aparecida S.; Balczun, Carsten; Oliveira, J. Henrique M.; Goncalves, Renata L. S.; Lazoski, Cristiano; Rivera Pomar, Rolando; Diambra, Luis Anibal; Schaub, Gunter A.; Garcia, Eloi S.; Azambuja, Patricia; Braz, Gloria R. C.; Oliveira, Pedro L.
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The bloodsucking hemipteran Rhodnius prolixus is a vector of Chagas’ disease, which affects 7–8 million people today in Latin America. In contrast to other hematophagous insects, the triatomine gut is compartmentalized into three segments that perform different functions during blood digestion. Here we report analysis of transcriptomes for each of the segments using pyrosequencing technology. Comparison of transcript frequency in digestive libraries with a whole-body library was used to evaluate expression levels. All classes of digestive enzymes were highly expressed, with a predominance of cysteine and aspartic proteinases, the latter showing a significant expansion through gene duplication. Although no protein digestion is known to occur in the anterior midgut (AM), protease transcripts were found, suggesting secretion as proenzymes, being possibly activated in the posterior midgut (PM). As expected, genes related to cytoskeleton, protein synthesis apparatus, protein traffic, and secretion were abundantly transcribed. Despite the absence of a chitinous peritrophic membrane in hemipterans - which have instead a lipidic perimicrovillar membrane lining over midgut epithelia - several gut-specific peritrophin transcripts were found, suggesting that these proteins perform functions other than being a structural component of the peritrophic membrane. Among immunity-related transcripts, while lysozymes and lectins were the most highly expressed, several genes belonging to the Toll pathway - found at low levels in the gut of most insects - were identified, contrasting with a low abundance of transcripts from IMD and STAT pathways. Analysis of transcripts related to lipid metabolism indicates that lipids play multiple roles, being a major energy source, a substrate for perimicrovillar membrane formation, and a source for hydrocarbons possibly to produce the wax layer of the hindgut. Transcripts related to amino acid metabolism showed an unanticipated priority for degradation of tyrosine, phenylalanine, and tryptophan. Analysis of transcripts related to signaling pathways suggested a role for MAP kinases, GTPases, and LKBP1/ AMP kinases related to control of cell shape and polarity, possibly in connection with regulation of cell survival, response of pathogens and nutrients. Together, our findings present a new view of the triatomine digestive apparatus and will help us understand trypanosome interaction and allow insights into hemipteran metabolic adaptations to a blood-based diet.
Fil: Ribeiro, José M. C.. National Institutes of Health; Estados Unidos
Fil: Genta, Fernando A.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Sorgine, Marcos H. F.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Mesquita, Rafael D.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Paiva Silva, Gabriela O.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Majerowicz, David. Universidade Federal do Rio de Janeiro; Brasil
Fil: Medeiros, Marcelo. Universidade Federal do Rio de Janeiro; Brasil
Fil: Koerich, Leonardo. Universidade Federal do Rio de Janeiro; Brasil
Fil: Terra, Walter R.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Ferreira, Clelia. Universidade Federal do Rio de Janeiro; Brasil
Fil: Pimentel, Andre C.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Bisch, Paulo M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Leite, Daniel C.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Diniz, Michelle M. P.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Da Silva, Manuela L.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Araujo, Ricardo N.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Gandara, Ana Caroline P.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Brosson, Sebastien. Universite Libre de Bruxelles; Bélgica
Fil: Salmon, Didier. Universidade Federal do Rio de Janeiro; Brasil
Fil: Bousbata, Sabrina. Universite Libre de Bruxelles; Bélgica
Fil: Gonzalez Caballero, Natalia. Universidade Federal do Rio de Janeiro; Brasil
Fil: Alves Bezerra, Michele. Universidade Federal do Rio de Janeiro; Brasil
Fil: Katia C. Gondim. Universidade Federal do Rio de Janeiro; Brasil
Fil: Silva Neto, Mario Alberto C.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Atella, Georgia C.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Araujo, Helena. Universidade Federal do Rio de Janeiro; Brasil
Fil: Dias, Felipe A.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Polycarpo, Carla. Universidade Federal do Rio de Janeiro; Brasil
Fil: Vionette Amaral, Raquel J.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Fampa, Patricia. Universidade Federal do Rio de Janeiro; Brasil
Fil: Melo, Ana Claudia A.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Tanaka, Aparecida S.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Balczun, Carsten. Universidade Federal do Rio de Janeiro; Brasil
Fil: Oliveira, J. Henrique M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Goncalves, Renata L. S.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Lazoski, Cristiano. Universidade Federal do Rio de Janeiro; Brasil
Fil: Rivera Pomar, Rolando. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Universidad Nacional del Noroeste de Buenos Aires. Centro de Bioinvestigaciones. Pergaminmo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diambra, Luis Anibal. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schaub, Gunter A.. Ruhr-Universität Bochum; Alemania
Fil: Garcia, Eloi S.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Azambuja, Patricia. Universidade Federal do Rio de Janeiro; Brasil
Fil: Braz, Gloria R. C.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; Brasil - Materia
-
TRANSCRIPTOMIC
RHODNIUS PROLIXUS
ANNOTATION
DIGESTIVE TRACT - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/24705
Ver los metadatos del registro completo
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An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixusRibeiro, José M. C.Genta, Fernando A.Sorgine, Marcos H. F.Mesquita, Rafael D.Paiva Silva, Gabriela O.Majerowicz, DavidMedeiros, MarceloKoerich, LeonardoTerra, Walter R.Ferreira, CleliaPimentel, Andre C.Bisch, Paulo M.Leite, Daniel C.Diniz, Michelle M. P.Da Silva, Manuela L.Araujo, Ricardo N.Gandara, Ana Caroline P.Brosson, SebastienSalmon, DidierBousbata, SabrinaGonzalez Caballero, NataliaAlves Bezerra, MicheleKatia C. GondimSilva Neto, Mario Alberto C.Atella, Georgia C.Araujo, HelenaDias, Felipe A.Polycarpo, CarlaVionette Amaral, Raquel J.Fampa, PatriciaMelo, Ana Claudia A.Tanaka, Aparecida S.Balczun, CarstenOliveira, J. Henrique M.Goncalves, Renata L. S.Lazoski, CristianoRivera Pomar, RolandoDiambra, Luis AnibalSchaub, Gunter A.Garcia, Eloi S.Azambuja, PatriciaBraz, Gloria R. C.Oliveira, Pedro L.TRANSCRIPTOMICRHODNIUS PROLIXUSANNOTATIONDIGESTIVE TRACThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The bloodsucking hemipteran Rhodnius prolixus is a vector of Chagas’ disease, which affects 7–8 million people today in Latin America. In contrast to other hematophagous insects, the triatomine gut is compartmentalized into three segments that perform different functions during blood digestion. Here we report analysis of transcriptomes for each of the segments using pyrosequencing technology. Comparison of transcript frequency in digestive libraries with a whole-body library was used to evaluate expression levels. All classes of digestive enzymes were highly expressed, with a predominance of cysteine and aspartic proteinases, the latter showing a significant expansion through gene duplication. Although no protein digestion is known to occur in the anterior midgut (AM), protease transcripts were found, suggesting secretion as proenzymes, being possibly activated in the posterior midgut (PM). As expected, genes related to cytoskeleton, protein synthesis apparatus, protein traffic, and secretion were abundantly transcribed. Despite the absence of a chitinous peritrophic membrane in hemipterans - which have instead a lipidic perimicrovillar membrane lining over midgut epithelia - several gut-specific peritrophin transcripts were found, suggesting that these proteins perform functions other than being a structural component of the peritrophic membrane. Among immunity-related transcripts, while lysozymes and lectins were the most highly expressed, several genes belonging to the Toll pathway - found at low levels in the gut of most insects - were identified, contrasting with a low abundance of transcripts from IMD and STAT pathways. Analysis of transcripts related to lipid metabolism indicates that lipids play multiple roles, being a major energy source, a substrate for perimicrovillar membrane formation, and a source for hydrocarbons possibly to produce the wax layer of the hindgut. Transcripts related to amino acid metabolism showed an unanticipated priority for degradation of tyrosine, phenylalanine, and tryptophan. Analysis of transcripts related to signaling pathways suggested a role for MAP kinases, GTPases, and LKBP1/ AMP kinases related to control of cell shape and polarity, possibly in connection with regulation of cell survival, response of pathogens and nutrients. Together, our findings present a new view of the triatomine digestive apparatus and will help us understand trypanosome interaction and allow insights into hemipteran metabolic adaptations to a blood-based diet.Fil: Ribeiro, José M. C.. National Institutes of Health; Estados UnidosFil: Genta, Fernando A.. Universidade Federal do Rio de Janeiro; BrasilFil: Sorgine, Marcos H. F.. Universidade Federal do Rio de Janeiro; BrasilFil: Mesquita, Rafael D.. Universidade Federal do Rio de Janeiro; BrasilFil: Paiva Silva, Gabriela O.. Universidade Federal do Rio de Janeiro; BrasilFil: Majerowicz, David. Universidade Federal do Rio de Janeiro; BrasilFil: Medeiros, Marcelo. Universidade Federal do Rio de Janeiro; BrasilFil: Koerich, Leonardo. Universidade Federal do Rio de Janeiro; BrasilFil: Terra, Walter R.. Universidade Federal do Rio de Janeiro; BrasilFil: Ferreira, Clelia. Universidade Federal do Rio de Janeiro; BrasilFil: Pimentel, Andre C.. Universidade Federal do Rio de Janeiro; BrasilFil: Bisch, Paulo M.. Universidade Federal do Rio de Janeiro; BrasilFil: Leite, Daniel C.. Universidade Federal do Rio de Janeiro; BrasilFil: Diniz, Michelle M. P.. Universidade Federal do Rio de Janeiro; BrasilFil: Da Silva, Manuela L.. Universidade Federal do Rio de Janeiro; BrasilFil: Araujo, Ricardo N.. Universidade Federal do Rio de Janeiro; BrasilFil: Gandara, Ana Caroline P.. Universidade Federal do Rio de Janeiro; BrasilFil: Brosson, Sebastien. Universite Libre de Bruxelles; BélgicaFil: Salmon, Didier. Universidade Federal do Rio de Janeiro; BrasilFil: Bousbata, Sabrina. Universite Libre de Bruxelles; BélgicaFil: Gonzalez Caballero, Natalia. Universidade Federal do Rio de Janeiro; BrasilFil: Alves Bezerra, Michele. Universidade Federal do Rio de Janeiro; BrasilFil: Katia C. Gondim. Universidade Federal do Rio de Janeiro; BrasilFil: Silva Neto, Mario Alberto C.. Universidade Federal do Rio de Janeiro; BrasilFil: Atella, Georgia C.. Universidade Federal do Rio de Janeiro; BrasilFil: Araujo, Helena. Universidade Federal do Rio de Janeiro; BrasilFil: Dias, Felipe A.. Universidade Federal do Rio de Janeiro; BrasilFil: Polycarpo, Carla. Universidade Federal do Rio de Janeiro; BrasilFil: Vionette Amaral, Raquel J.. Universidade Federal do Rio de Janeiro; BrasilFil: Fampa, Patricia. Universidade Federal do Rio de Janeiro; BrasilFil: Melo, Ana Claudia A.. Universidade Federal do Rio de Janeiro; BrasilFil: Tanaka, Aparecida S.. Universidade Federal do Rio de Janeiro; BrasilFil: Balczun, Carsten. Universidade Federal do Rio de Janeiro; BrasilFil: Oliveira, J. Henrique M.. Universidade Federal do Rio de Janeiro; BrasilFil: Goncalves, Renata L. S.. Universidade Federal do Rio de Janeiro; BrasilFil: Lazoski, Cristiano. Universidade Federal do Rio de Janeiro; BrasilFil: Rivera Pomar, Rolando. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Universidad Nacional del Noroeste de Buenos Aires. Centro de Bioinvestigaciones. Pergaminmo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Diambra, Luis Anibal. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schaub, Gunter A.. Ruhr-Universität Bochum; AlemaniaFil: Garcia, Eloi S.. Universidade Federal do Rio de Janeiro; BrasilFil: Azambuja, Patricia. Universidade Federal do Rio de Janeiro; BrasilFil: Braz, Gloria R. C.. Universidade Federal do Rio de Janeiro; BrasilFil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; BrasilPublic Library of Science2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24705Ribeiro, José M. C.; Genta, Fernando A.; Sorgine, Marcos H. F.; Mesquita, Rafael D.; Paiva Silva, Gabriela O.; et al.; An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus; Public Library of Science; Neglected Tropical Diseases; 8; 1; 1-2014; 259401-e2594311935-2735CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0002594info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0002594info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:00Zoai:ri.conicet.gov.ar:11336/24705instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:01.195CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus |
| title |
An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus |
| spellingShingle |
An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus Ribeiro, José M. C. TRANSCRIPTOMIC RHODNIUS PROLIXUS ANNOTATION DIGESTIVE TRACT |
| title_short |
An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus |
| title_full |
An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus |
| title_fullStr |
An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus |
| title_full_unstemmed |
An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus |
| title_sort |
An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus |
| dc.creator.none.fl_str_mv |
Ribeiro, José M. C. Genta, Fernando A. Sorgine, Marcos H. F. Mesquita, Rafael D. Paiva Silva, Gabriela O. Majerowicz, David Medeiros, Marcelo Koerich, Leonardo Terra, Walter R. Ferreira, Clelia Pimentel, Andre C. Bisch, Paulo M. Leite, Daniel C. Diniz, Michelle M. P. Da Silva, Manuela L. Araujo, Ricardo N. Gandara, Ana Caroline P. Brosson, Sebastien Salmon, Didier Bousbata, Sabrina Gonzalez Caballero, Natalia Alves Bezerra, Michele Katia C. Gondim Silva Neto, Mario Alberto C. Atella, Georgia C. Araujo, Helena Dias, Felipe A. Polycarpo, Carla Vionette Amaral, Raquel J. Fampa, Patricia Melo, Ana Claudia A. Tanaka, Aparecida S. Balczun, Carsten Oliveira, J. Henrique M. Goncalves, Renata L. S. Lazoski, Cristiano Rivera Pomar, Rolando Diambra, Luis Anibal Schaub, Gunter A. Garcia, Eloi S. Azambuja, Patricia Braz, Gloria R. C. Oliveira, Pedro L. |
| author |
Ribeiro, José M. C. |
| author_facet |
Ribeiro, José M. C. Genta, Fernando A. Sorgine, Marcos H. F. Mesquita, Rafael D. Paiva Silva, Gabriela O. Majerowicz, David Medeiros, Marcelo Koerich, Leonardo Terra, Walter R. Ferreira, Clelia Pimentel, Andre C. Bisch, Paulo M. Leite, Daniel C. Diniz, Michelle M. P. Da Silva, Manuela L. Araujo, Ricardo N. Gandara, Ana Caroline P. Brosson, Sebastien Salmon, Didier Bousbata, Sabrina Gonzalez Caballero, Natalia Alves Bezerra, Michele Katia C. Gondim Silva Neto, Mario Alberto C. Atella, Georgia C. Araujo, Helena Dias, Felipe A. Polycarpo, Carla Vionette Amaral, Raquel J. Fampa, Patricia Melo, Ana Claudia A. Tanaka, Aparecida S. Balczun, Carsten Oliveira, J. Henrique M. Goncalves, Renata L. S. Lazoski, Cristiano Rivera Pomar, Rolando Diambra, Luis Anibal Schaub, Gunter A. Garcia, Eloi S. Azambuja, Patricia Braz, Gloria R. C. Oliveira, Pedro L. |
| author_role |
author |
| author2 |
Genta, Fernando A. Sorgine, Marcos H. F. Mesquita, Rafael D. Paiva Silva, Gabriela O. Majerowicz, David Medeiros, Marcelo Koerich, Leonardo Terra, Walter R. Ferreira, Clelia Pimentel, Andre C. Bisch, Paulo M. Leite, Daniel C. Diniz, Michelle M. P. Da Silva, Manuela L. Araujo, Ricardo N. Gandara, Ana Caroline P. Brosson, Sebastien Salmon, Didier Bousbata, Sabrina Gonzalez Caballero, Natalia Alves Bezerra, Michele Katia C. Gondim Silva Neto, Mario Alberto C. Atella, Georgia C. Araujo, Helena Dias, Felipe A. Polycarpo, Carla Vionette Amaral, Raquel J. Fampa, Patricia Melo, Ana Claudia A. Tanaka, Aparecida S. Balczun, Carsten Oliveira, J. Henrique M. Goncalves, Renata L. S. Lazoski, Cristiano Rivera Pomar, Rolando Diambra, Luis Anibal Schaub, Gunter A. Garcia, Eloi S. Azambuja, Patricia Braz, Gloria R. C. Oliveira, Pedro L. |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
TRANSCRIPTOMIC RHODNIUS PROLIXUS ANNOTATION DIGESTIVE TRACT |
| topic |
TRANSCRIPTOMIC RHODNIUS PROLIXUS ANNOTATION DIGESTIVE TRACT |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
The bloodsucking hemipteran Rhodnius prolixus is a vector of Chagas’ disease, which affects 7–8 million people today in Latin America. In contrast to other hematophagous insects, the triatomine gut is compartmentalized into three segments that perform different functions during blood digestion. Here we report analysis of transcriptomes for each of the segments using pyrosequencing technology. Comparison of transcript frequency in digestive libraries with a whole-body library was used to evaluate expression levels. All classes of digestive enzymes were highly expressed, with a predominance of cysteine and aspartic proteinases, the latter showing a significant expansion through gene duplication. Although no protein digestion is known to occur in the anterior midgut (AM), protease transcripts were found, suggesting secretion as proenzymes, being possibly activated in the posterior midgut (PM). As expected, genes related to cytoskeleton, protein synthesis apparatus, protein traffic, and secretion were abundantly transcribed. Despite the absence of a chitinous peritrophic membrane in hemipterans - which have instead a lipidic perimicrovillar membrane lining over midgut epithelia - several gut-specific peritrophin transcripts were found, suggesting that these proteins perform functions other than being a structural component of the peritrophic membrane. Among immunity-related transcripts, while lysozymes and lectins were the most highly expressed, several genes belonging to the Toll pathway - found at low levels in the gut of most insects - were identified, contrasting with a low abundance of transcripts from IMD and STAT pathways. Analysis of transcripts related to lipid metabolism indicates that lipids play multiple roles, being a major energy source, a substrate for perimicrovillar membrane formation, and a source for hydrocarbons possibly to produce the wax layer of the hindgut. Transcripts related to amino acid metabolism showed an unanticipated priority for degradation of tyrosine, phenylalanine, and tryptophan. Analysis of transcripts related to signaling pathways suggested a role for MAP kinases, GTPases, and LKBP1/ AMP kinases related to control of cell shape and polarity, possibly in connection with regulation of cell survival, response of pathogens and nutrients. Together, our findings present a new view of the triatomine digestive apparatus and will help us understand trypanosome interaction and allow insights into hemipteran metabolic adaptations to a blood-based diet. Fil: Ribeiro, José M. C.. National Institutes of Health; Estados Unidos Fil: Genta, Fernando A.. Universidade Federal do Rio de Janeiro; Brasil Fil: Sorgine, Marcos H. F.. Universidade Federal do Rio de Janeiro; Brasil Fil: Mesquita, Rafael D.. Universidade Federal do Rio de Janeiro; Brasil Fil: Paiva Silva, Gabriela O.. Universidade Federal do Rio de Janeiro; Brasil Fil: Majerowicz, David. Universidade Federal do Rio de Janeiro; Brasil Fil: Medeiros, Marcelo. Universidade Federal do Rio de Janeiro; Brasil Fil: Koerich, Leonardo. Universidade Federal do Rio de Janeiro; Brasil Fil: Terra, Walter R.. Universidade Federal do Rio de Janeiro; Brasil Fil: Ferreira, Clelia. Universidade Federal do Rio de Janeiro; Brasil Fil: Pimentel, Andre C.. Universidade Federal do Rio de Janeiro; Brasil Fil: Bisch, Paulo M.. Universidade Federal do Rio de Janeiro; Brasil Fil: Leite, Daniel C.. Universidade Federal do Rio de Janeiro; Brasil Fil: Diniz, Michelle M. P.. Universidade Federal do Rio de Janeiro; Brasil Fil: Da Silva, Manuela L.. Universidade Federal do Rio de Janeiro; Brasil Fil: Araujo, Ricardo N.. Universidade Federal do Rio de Janeiro; Brasil Fil: Gandara, Ana Caroline P.. Universidade Federal do Rio de Janeiro; Brasil Fil: Brosson, Sebastien. Universite Libre de Bruxelles; Bélgica Fil: Salmon, Didier. Universidade Federal do Rio de Janeiro; Brasil Fil: Bousbata, Sabrina. Universite Libre de Bruxelles; Bélgica Fil: Gonzalez Caballero, Natalia. Universidade Federal do Rio de Janeiro; Brasil Fil: Alves Bezerra, Michele. Universidade Federal do Rio de Janeiro; Brasil Fil: Katia C. Gondim. Universidade Federal do Rio de Janeiro; Brasil Fil: Silva Neto, Mario Alberto C.. Universidade Federal do Rio de Janeiro; Brasil Fil: Atella, Georgia C.. Universidade Federal do Rio de Janeiro; Brasil Fil: Araujo, Helena. Universidade Federal do Rio de Janeiro; Brasil Fil: Dias, Felipe A.. Universidade Federal do Rio de Janeiro; Brasil Fil: Polycarpo, Carla. Universidade Federal do Rio de Janeiro; Brasil Fil: Vionette Amaral, Raquel J.. Universidade Federal do Rio de Janeiro; Brasil Fil: Fampa, Patricia. Universidade Federal do Rio de Janeiro; Brasil Fil: Melo, Ana Claudia A.. Universidade Federal do Rio de Janeiro; Brasil Fil: Tanaka, Aparecida S.. Universidade Federal do Rio de Janeiro; Brasil Fil: Balczun, Carsten. Universidade Federal do Rio de Janeiro; Brasil Fil: Oliveira, J. Henrique M.. Universidade Federal do Rio de Janeiro; Brasil Fil: Goncalves, Renata L. S.. Universidade Federal do Rio de Janeiro; Brasil Fil: Lazoski, Cristiano. Universidade Federal do Rio de Janeiro; Brasil Fil: Rivera Pomar, Rolando. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Universidad Nacional del Noroeste de Buenos Aires. Centro de Bioinvestigaciones. Pergaminmo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Diambra, Luis Anibal. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schaub, Gunter A.. Ruhr-Universität Bochum; Alemania Fil: Garcia, Eloi S.. Universidade Federal do Rio de Janeiro; Brasil Fil: Azambuja, Patricia. Universidade Federal do Rio de Janeiro; Brasil Fil: Braz, Gloria R. C.. Universidade Federal do Rio de Janeiro; Brasil Fil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; Brasil |
| description |
The bloodsucking hemipteran Rhodnius prolixus is a vector of Chagas’ disease, which affects 7–8 million people today in Latin America. In contrast to other hematophagous insects, the triatomine gut is compartmentalized into three segments that perform different functions during blood digestion. Here we report analysis of transcriptomes for each of the segments using pyrosequencing technology. Comparison of transcript frequency in digestive libraries with a whole-body library was used to evaluate expression levels. All classes of digestive enzymes were highly expressed, with a predominance of cysteine and aspartic proteinases, the latter showing a significant expansion through gene duplication. Although no protein digestion is known to occur in the anterior midgut (AM), protease transcripts were found, suggesting secretion as proenzymes, being possibly activated in the posterior midgut (PM). As expected, genes related to cytoskeleton, protein synthesis apparatus, protein traffic, and secretion were abundantly transcribed. Despite the absence of a chitinous peritrophic membrane in hemipterans - which have instead a lipidic perimicrovillar membrane lining over midgut epithelia - several gut-specific peritrophin transcripts were found, suggesting that these proteins perform functions other than being a structural component of the peritrophic membrane. Among immunity-related transcripts, while lysozymes and lectins were the most highly expressed, several genes belonging to the Toll pathway - found at low levels in the gut of most insects - were identified, contrasting with a low abundance of transcripts from IMD and STAT pathways. Analysis of transcripts related to lipid metabolism indicates that lipids play multiple roles, being a major energy source, a substrate for perimicrovillar membrane formation, and a source for hydrocarbons possibly to produce the wax layer of the hindgut. Transcripts related to amino acid metabolism showed an unanticipated priority for degradation of tyrosine, phenylalanine, and tryptophan. Analysis of transcripts related to signaling pathways suggested a role for MAP kinases, GTPases, and LKBP1/ AMP kinases related to control of cell shape and polarity, possibly in connection with regulation of cell survival, response of pathogens and nutrients. Together, our findings present a new view of the triatomine digestive apparatus and will help us understand trypanosome interaction and allow insights into hemipteran metabolic adaptations to a blood-based diet. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014-01 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/24705 Ribeiro, José M. C.; Genta, Fernando A.; Sorgine, Marcos H. F.; Mesquita, Rafael D.; Paiva Silva, Gabriela O.; et al.; An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus; Public Library of Science; Neglected Tropical Diseases; 8; 1; 1-2014; 259401-e259431 1935-2735 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/24705 |
| identifier_str_mv |
Ribeiro, José M. C.; Genta, Fernando A.; Sorgine, Marcos H. F.; Mesquita, Rafael D.; Paiva Silva, Gabriela O.; et al.; An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus; Public Library of Science; Neglected Tropical Diseases; 8; 1; 1-2014; 259401-e259431 1935-2735 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0002594 info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0002594 |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Public Library of Science |
| publisher.none.fl_str_mv |
Public Library of Science |
| dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
| reponame_str |
CONICET Digital (CONICET) |
| collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
| repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
| repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613799011680256 |
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13.070432 |