Hyaluronic acid—dexamethasone nanoparticles for local adjunct therapy of lung inflammation

Autores
Cámara, Candelaria Inés; Bertocchi, Laura; Ricci, Caterina; Bassi, Rosaria; Bianchera, Annalisa; Cantú, Laura F.; Ruggero, Bettini; Del Favero, Elena
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The delivery of a dexamethasone formulation directly into the lung appears as an appro-priate strategy to strengthen the systemic administration, reducing the dosage in the treatment of lung severe inflammations. For this purpose, a hyaluronic acid-dexamethasone formulation was developed, affording an inhalable reconstituted nanosuspension suitable to be aerosolized. The physico-chemical and biopharmaceutical properties of the formulation were tested: size, stability, loading of the spray-dried dry powder, reconstitution capability upon redispersion in aqueous me-dia. Detailed structural insights on nanoparticles after reconstitution were obtained by light and X-ray scattering techniques. (1) The size of the nanoparticles, around 200 nm, is in the proper range for a possible engulfment by macrophages. (2) Their structure is of the core-shell type, hosting dex-amethasone nanocrystals inside and carrying hyaluronic acid chains on the surface. This specific structure allows for nanosuspension stability and provides nanoparticles with muco-inert proper-ties. (3) The nanosuspension can be efficiently aerosolized, allowing for a high drug fraction poten-tially reaching the deep lung. Thus, this formulation represents a promising tool for the lung administration via nebulization directly in the pipe of ventilators, to be used as such or as adjunct therapy for severe lung inflammation.
Fil: Cámara, Candelaria Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Bertocchi, Laura. Departamento de Alimentos y Drogas ; Universita Degli Studi Di Parma;
Fil: Ricci, Caterina. Universita Degli Studi Di Milano. Dipartimento Di Beitecnologe Mediche E Medicina Traslazionale.; Italia
Fil: Bassi, Rosaria. Universita Degli Studi Di Milano. Dipartimento Di Beitecnologe Mediche E Medicina Traslazionale.; Italia
Fil: Bianchera, Annalisa. Departamento de Alimentos y Drogas ; Universita Degli Studi Di Parma;
Fil: Cantú, Laura F.. Universita Degli Studi Di Milano. Dipartimento Di Beitecnologe Mediche E Medicina Traslazionale.; Italia
Fil: Ruggero, Bettini. Departamento de Alimentos y Drogas ; Universita Degli Studi Di Parma;
Fil: Del Favero, Elena. Universita Degli Studi Di Milano. Dipartimento Di Beitecnologe Mediche E Medicina Traslazionale.; Italia
Materia
DRUG DELIVERY
LIGHT SCATTERING
LUNG AD-MINISTRATION
MUCUS INTERACTION
NANOCRYSTALS
NANOPARTICLE CHARACTERIZATION
X-RAY SCATTERING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/172961
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hyaluronic acid—dexamethasone nanoparticles for local adjunct therapy of lung inflammationCámara, Candelaria InésBertocchi, LauraRicci, CaterinaBassi, RosariaBianchera, AnnalisaCantú, Laura F.Ruggero, BettiniDel Favero, ElenaDRUG DELIVERYLIGHT SCATTERINGLUNG AD-MINISTRATIONMUCUS INTERACTIONNANOCRYSTALSNANOPARTICLE CHARACTERIZATIONX-RAY SCATTERINGhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The delivery of a dexamethasone formulation directly into the lung appears as an appro-priate strategy to strengthen the systemic administration, reducing the dosage in the treatment of lung severe inflammations. For this purpose, a hyaluronic acid-dexamethasone formulation was developed, affording an inhalable reconstituted nanosuspension suitable to be aerosolized. The physico-chemical and biopharmaceutical properties of the formulation were tested: size, stability, loading of the spray-dried dry powder, reconstitution capability upon redispersion in aqueous me-dia. Detailed structural insights on nanoparticles after reconstitution were obtained by light and X-ray scattering techniques. (1) The size of the nanoparticles, around 200 nm, is in the proper range for a possible engulfment by macrophages. (2) Their structure is of the core-shell type, hosting dex-amethasone nanocrystals inside and carrying hyaluronic acid chains on the surface. This specific structure allows for nanosuspension stability and provides nanoparticles with muco-inert proper-ties. (3) The nanosuspension can be efficiently aerosolized, allowing for a high drug fraction poten-tially reaching the deep lung. Thus, this formulation represents a promising tool for the lung administration via nebulization directly in the pipe of ventilators, to be used as such or as adjunct therapy for severe lung inflammation.Fil: Cámara, Candelaria Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Bertocchi, Laura. Departamento de Alimentos y Drogas ; Universita Degli Studi Di Parma;Fil: Ricci, Caterina. Universita Degli Studi Di Milano. Dipartimento Di Beitecnologe Mediche E Medicina Traslazionale.; ItaliaFil: Bassi, Rosaria. Universita Degli Studi Di Milano. Dipartimento Di Beitecnologe Mediche E Medicina Traslazionale.; ItaliaFil: Bianchera, Annalisa. Departamento de Alimentos y Drogas ; Universita Degli Studi Di Parma;Fil: Cantú, Laura F.. Universita Degli Studi Di Milano. Dipartimento Di Beitecnologe Mediche E Medicina Traslazionale.; ItaliaFil: Ruggero, Bettini. Departamento de Alimentos y Drogas ; Universita Degli Studi Di Parma;Fil: Del Favero, Elena. Universita Degli Studi Di Milano. Dipartimento Di Beitecnologe Mediche E Medicina Traslazionale.; ItaliaMolecular Diversity Preservation International2021-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/172961Cámara, Candelaria Inés; Bertocchi, Laura; Ricci, Caterina; Bassi, Rosaria; Bianchera, Annalisa; et al.; Hyaluronic acid—dexamethasone nanoparticles for local adjunct therapy of lung inflammation; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 22; 19; 10-2021; 1-181422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/22/19/10480info:eu-repo/semantics/altIdentifier/doi/ 10.3390/ijms221910480info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:45:58Zoai:ri.conicet.gov.ar:11336/172961instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:45:59.031CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hyaluronic acid—dexamethasone nanoparticles for local adjunct therapy of lung inflammation
title Hyaluronic acid—dexamethasone nanoparticles for local adjunct therapy of lung inflammation
spellingShingle Hyaluronic acid—dexamethasone nanoparticles for local adjunct therapy of lung inflammation
Cámara, Candelaria Inés
DRUG DELIVERY
LIGHT SCATTERING
LUNG AD-MINISTRATION
MUCUS INTERACTION
NANOCRYSTALS
NANOPARTICLE CHARACTERIZATION
X-RAY SCATTERING
title_short Hyaluronic acid—dexamethasone nanoparticles for local adjunct therapy of lung inflammation
title_full Hyaluronic acid—dexamethasone nanoparticles for local adjunct therapy of lung inflammation
title_fullStr Hyaluronic acid—dexamethasone nanoparticles for local adjunct therapy of lung inflammation
title_full_unstemmed Hyaluronic acid—dexamethasone nanoparticles for local adjunct therapy of lung inflammation
title_sort Hyaluronic acid—dexamethasone nanoparticles for local adjunct therapy of lung inflammation
dc.creator.none.fl_str_mv Cámara, Candelaria Inés
Bertocchi, Laura
Ricci, Caterina
Bassi, Rosaria
Bianchera, Annalisa
Cantú, Laura F.
Ruggero, Bettini
Del Favero, Elena
author Cámara, Candelaria Inés
author_facet Cámara, Candelaria Inés
Bertocchi, Laura
Ricci, Caterina
Bassi, Rosaria
Bianchera, Annalisa
Cantú, Laura F.
Ruggero, Bettini
Del Favero, Elena
author_role author
author2 Bertocchi, Laura
Ricci, Caterina
Bassi, Rosaria
Bianchera, Annalisa
Cantú, Laura F.
Ruggero, Bettini
Del Favero, Elena
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DRUG DELIVERY
LIGHT SCATTERING
LUNG AD-MINISTRATION
MUCUS INTERACTION
NANOCRYSTALS
NANOPARTICLE CHARACTERIZATION
X-RAY SCATTERING
topic DRUG DELIVERY
LIGHT SCATTERING
LUNG AD-MINISTRATION
MUCUS INTERACTION
NANOCRYSTALS
NANOPARTICLE CHARACTERIZATION
X-RAY SCATTERING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The delivery of a dexamethasone formulation directly into the lung appears as an appro-priate strategy to strengthen the systemic administration, reducing the dosage in the treatment of lung severe inflammations. For this purpose, a hyaluronic acid-dexamethasone formulation was developed, affording an inhalable reconstituted nanosuspension suitable to be aerosolized. The physico-chemical and biopharmaceutical properties of the formulation were tested: size, stability, loading of the spray-dried dry powder, reconstitution capability upon redispersion in aqueous me-dia. Detailed structural insights on nanoparticles after reconstitution were obtained by light and X-ray scattering techniques. (1) The size of the nanoparticles, around 200 nm, is in the proper range for a possible engulfment by macrophages. (2) Their structure is of the core-shell type, hosting dex-amethasone nanocrystals inside and carrying hyaluronic acid chains on the surface. This specific structure allows for nanosuspension stability and provides nanoparticles with muco-inert proper-ties. (3) The nanosuspension can be efficiently aerosolized, allowing for a high drug fraction poten-tially reaching the deep lung. Thus, this formulation represents a promising tool for the lung administration via nebulization directly in the pipe of ventilators, to be used as such or as adjunct therapy for severe lung inflammation.
Fil: Cámara, Candelaria Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Bertocchi, Laura. Departamento de Alimentos y Drogas ; Universita Degli Studi Di Parma;
Fil: Ricci, Caterina. Universita Degli Studi Di Milano. Dipartimento Di Beitecnologe Mediche E Medicina Traslazionale.; Italia
Fil: Bassi, Rosaria. Universita Degli Studi Di Milano. Dipartimento Di Beitecnologe Mediche E Medicina Traslazionale.; Italia
Fil: Bianchera, Annalisa. Departamento de Alimentos y Drogas ; Universita Degli Studi Di Parma;
Fil: Cantú, Laura F.. Universita Degli Studi Di Milano. Dipartimento Di Beitecnologe Mediche E Medicina Traslazionale.; Italia
Fil: Ruggero, Bettini. Departamento de Alimentos y Drogas ; Universita Degli Studi Di Parma;
Fil: Del Favero, Elena. Universita Degli Studi Di Milano. Dipartimento Di Beitecnologe Mediche E Medicina Traslazionale.; Italia
description The delivery of a dexamethasone formulation directly into the lung appears as an appro-priate strategy to strengthen the systemic administration, reducing the dosage in the treatment of lung severe inflammations. For this purpose, a hyaluronic acid-dexamethasone formulation was developed, affording an inhalable reconstituted nanosuspension suitable to be aerosolized. The physico-chemical and biopharmaceutical properties of the formulation were tested: size, stability, loading of the spray-dried dry powder, reconstitution capability upon redispersion in aqueous me-dia. Detailed structural insights on nanoparticles after reconstitution were obtained by light and X-ray scattering techniques. (1) The size of the nanoparticles, around 200 nm, is in the proper range for a possible engulfment by macrophages. (2) Their structure is of the core-shell type, hosting dex-amethasone nanocrystals inside and carrying hyaluronic acid chains on the surface. This specific structure allows for nanosuspension stability and provides nanoparticles with muco-inert proper-ties. (3) The nanosuspension can be efficiently aerosolized, allowing for a high drug fraction poten-tially reaching the deep lung. Thus, this formulation represents a promising tool for the lung administration via nebulization directly in the pipe of ventilators, to be used as such or as adjunct therapy for severe lung inflammation.
publishDate 2021
dc.date.none.fl_str_mv 2021-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/172961
Cámara, Candelaria Inés; Bertocchi, Laura; Ricci, Caterina; Bassi, Rosaria; Bianchera, Annalisa; et al.; Hyaluronic acid—dexamethasone nanoparticles for local adjunct therapy of lung inflammation; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 22; 19; 10-2021; 1-18
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/172961
identifier_str_mv Cámara, Candelaria Inés; Bertocchi, Laura; Ricci, Caterina; Bassi, Rosaria; Bianchera, Annalisa; et al.; Hyaluronic acid—dexamethasone nanoparticles for local adjunct therapy of lung inflammation; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 22; 19; 10-2021; 1-18
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/22/19/10480
info:eu-repo/semantics/altIdentifier/doi/ 10.3390/ijms221910480
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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