Association with amino acids does not enhance efficacy of polymerized liposomes as a system for lung gene delivery

Autores
Bandeira, Elga; Lopes Pacheco, Miquéias; Chiaramoni, Nadia Silvia; Ferreira, Débora; Fernandez Ruocco, Maria Julieta; Prieto, Maria Jimena; Maron Gutierrez, Tatiana; Perrotta, Ramiro Martin; de Castro Faria Neto, Hugo C.; Rocco, Patricia R. M.; Alonso, Silvia del Valle; Morales, Marcelo M.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Development of improved drug and gene delivery systems directly into the lungs is highly desirable given the important burden of respiratory diseases. We aimed to evaluate the safety and efficacy of liposomes composed of photopolymerized lipids [1,2-bis-(tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine] associated with amino acids as vectors for gene delivery into the lungs of healthy animals. Lipopolymer vesicles, in particular, are more stable than other types of liposomes. In this study, lipopolymers were associated with L-arginine, L-tryptophan, or L-cysteine. We hypothesized that the addition of these amino acids would enhance the efficacy of gene delivery to the lungs by the lipopolymers. L-Arginine showed the highest association efficiency due to its positive charge and better surface interactions. None of the formulations caused inflammation or altered lung mechanics, suggesting that these lipopolymers can be safely administered as aerosols. All formulations were able to induce eGFP mRNA expression in lung tissue, but the addition of amino acids reduced delivery efficacy when compared with the simple lipopolymer particle. These results indicate that this system could be further explored for gene or drug delivery targeting lung diseases.
Fil: Bandeira, Elga. Universidade Federal do Rio de Janeiro; Brasil
Fil: Lopes Pacheco, Miquéias. Universidade Federal do Rio de Janeiro; Brasil
Fil: Chiaramoni, Nadia Silvia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferreira, Débora. Universidade Federal do Rio de Janeiro; Brasil
Fil: Fernandez Ruocco, Maria Julieta. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina
Fil: Prieto, Maria Jimena. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maron Gutierrez, Tatiana. Fundación Oswaldo Cruz; Brasil
Fil: Perrotta, Ramiro Martin. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Castro Faria Neto, Hugo C.. Fundación Oswaldo Cruz; Brasil
Fil: Rocco, Patricia R. M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Alonso, Silvia del Valle. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Morales, Marcelo M.. Universidade Federal do Rio de Janeiro; Brasil
Materia
LUNG
GENE DELIVERY
MECHANIC PARAMETERS
NANOPARTICLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54323
Acceder
id CONICETDig_f333b3123f0b0d100908b12d7d8ad2b1
oai_identifier_str oai:ri.conicet.gov.ar:11336/54323
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Association with amino acids does not enhance efficacy of polymerized liposomes as a system for lung gene deliveryBandeira, ElgaLopes Pacheco, MiquéiasChiaramoni, Nadia SilviaFerreira, DéboraFernandez Ruocco, Maria JulietaPrieto, Maria JimenaMaron Gutierrez, TatianaPerrotta, Ramiro Martinde Castro Faria Neto, Hugo C.Rocco, Patricia R. M.Alonso, Silvia del ValleMorales, Marcelo M.LUNGGENE DELIVERYMECHANIC PARAMETERSNANOPARTICLEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Development of improved drug and gene delivery systems directly into the lungs is highly desirable given the important burden of respiratory diseases. We aimed to evaluate the safety and efficacy of liposomes composed of photopolymerized lipids [1,2-bis-(tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine] associated with amino acids as vectors for gene delivery into the lungs of healthy animals. Lipopolymer vesicles, in particular, are more stable than other types of liposomes. In this study, lipopolymers were associated with L-arginine, L-tryptophan, or L-cysteine. We hypothesized that the addition of these amino acids would enhance the efficacy of gene delivery to the lungs by the lipopolymers. L-Arginine showed the highest association efficiency due to its positive charge and better surface interactions. None of the formulations caused inflammation or altered lung mechanics, suggesting that these lipopolymers can be safely administered as aerosols. All formulations were able to induce eGFP mRNA expression in lung tissue, but the addition of amino acids reduced delivery efficacy when compared with the simple lipopolymer particle. These results indicate that this system could be further explored for gene or drug delivery targeting lung diseases.Fil: Bandeira, Elga. Universidade Federal do Rio de Janeiro; BrasilFil: Lopes Pacheco, Miquéias. Universidade Federal do Rio de Janeiro; BrasilFil: Chiaramoni, Nadia Silvia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferreira, Débora. Universidade Federal do Rio de Janeiro; BrasilFil: Fernandez Ruocco, Maria Julieta. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; ArgentinaFil: Prieto, Maria Jimena. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maron Gutierrez, Tatiana. Fundación Oswaldo Cruz; BrasilFil: Perrotta, Ramiro Martin. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: de Castro Faria Neto, Hugo C.. Fundación Oswaldo Cruz; BrasilFil: Rocco, Patricia R. M.. Universidade Federal do Rio de Janeiro; BrasilFil: Alonso, Silvia del Valle. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Morales, Marcelo M.. Universidade Federal do Rio de Janeiro; BrasilFrontiers Research Foundation2016-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54323Bandeira, Elga; Lopes Pacheco, Miquéias; Chiaramoni, Nadia Silvia; Ferreira, Débora; Fernandez Ruocco, Maria Julieta; et al.; Association with amino acids does not enhance efficacy of polymerized liposomes as a system for lung gene delivery; Frontiers Research Foundation; Frontiers in Physiology; 7; 151; 4-2016; 1-91664-042XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fphys.2016.00151info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fphys.2016.00151/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:47Zoai:ri.conicet.gov.ar:11336/54323instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:48.054CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Association with amino acids does not enhance efficacy of polymerized liposomes as a system for lung gene delivery
title Association with amino acids does not enhance efficacy of polymerized liposomes as a system for lung gene delivery
spellingShingle Association with amino acids does not enhance efficacy of polymerized liposomes as a system for lung gene delivery
Bandeira, Elga
LUNG
GENE DELIVERY
MECHANIC PARAMETERS
NANOPARTICLES
title_short Association with amino acids does not enhance efficacy of polymerized liposomes as a system for lung gene delivery
title_full Association with amino acids does not enhance efficacy of polymerized liposomes as a system for lung gene delivery
title_fullStr Association with amino acids does not enhance efficacy of polymerized liposomes as a system for lung gene delivery
title_full_unstemmed Association with amino acids does not enhance efficacy of polymerized liposomes as a system for lung gene delivery
title_sort Association with amino acids does not enhance efficacy of polymerized liposomes as a system for lung gene delivery
dc.creator.none.fl_str_mv Bandeira, Elga
Lopes Pacheco, Miquéias
Chiaramoni, Nadia Silvia
Ferreira, Débora
Fernandez Ruocco, Maria Julieta
Prieto, Maria Jimena
Maron Gutierrez, Tatiana
Perrotta, Ramiro Martin
de Castro Faria Neto, Hugo C.
Rocco, Patricia R. M.
Alonso, Silvia del Valle
Morales, Marcelo M.
author Bandeira, Elga
author_facet Bandeira, Elga
Lopes Pacheco, Miquéias
Chiaramoni, Nadia Silvia
Ferreira, Débora
Fernandez Ruocco, Maria Julieta
Prieto, Maria Jimena
Maron Gutierrez, Tatiana
Perrotta, Ramiro Martin
de Castro Faria Neto, Hugo C.
Rocco, Patricia R. M.
Alonso, Silvia del Valle
Morales, Marcelo M.
author_role author
author2 Lopes Pacheco, Miquéias
Chiaramoni, Nadia Silvia
Ferreira, Débora
Fernandez Ruocco, Maria Julieta
Prieto, Maria Jimena
Maron Gutierrez, Tatiana
Perrotta, Ramiro Martin
de Castro Faria Neto, Hugo C.
Rocco, Patricia R. M.
Alonso, Silvia del Valle
Morales, Marcelo M.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LUNG
GENE DELIVERY
MECHANIC PARAMETERS
NANOPARTICLES
topic LUNG
GENE DELIVERY
MECHANIC PARAMETERS
NANOPARTICLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Development of improved drug and gene delivery systems directly into the lungs is highly desirable given the important burden of respiratory diseases. We aimed to evaluate the safety and efficacy of liposomes composed of photopolymerized lipids [1,2-bis-(tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine] associated with amino acids as vectors for gene delivery into the lungs of healthy animals. Lipopolymer vesicles, in particular, are more stable than other types of liposomes. In this study, lipopolymers were associated with L-arginine, L-tryptophan, or L-cysteine. We hypothesized that the addition of these amino acids would enhance the efficacy of gene delivery to the lungs by the lipopolymers. L-Arginine showed the highest association efficiency due to its positive charge and better surface interactions. None of the formulations caused inflammation or altered lung mechanics, suggesting that these lipopolymers can be safely administered as aerosols. All formulations were able to induce eGFP mRNA expression in lung tissue, but the addition of amino acids reduced delivery efficacy when compared with the simple lipopolymer particle. These results indicate that this system could be further explored for gene or drug delivery targeting lung diseases.
Fil: Bandeira, Elga. Universidade Federal do Rio de Janeiro; Brasil
Fil: Lopes Pacheco, Miquéias. Universidade Federal do Rio de Janeiro; Brasil
Fil: Chiaramoni, Nadia Silvia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferreira, Débora. Universidade Federal do Rio de Janeiro; Brasil
Fil: Fernandez Ruocco, Maria Julieta. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina
Fil: Prieto, Maria Jimena. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maron Gutierrez, Tatiana. Fundación Oswaldo Cruz; Brasil
Fil: Perrotta, Ramiro Martin. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Castro Faria Neto, Hugo C.. Fundación Oswaldo Cruz; Brasil
Fil: Rocco, Patricia R. M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Alonso, Silvia del Valle. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Morales, Marcelo M.. Universidade Federal do Rio de Janeiro; Brasil
description Development of improved drug and gene delivery systems directly into the lungs is highly desirable given the important burden of respiratory diseases. We aimed to evaluate the safety and efficacy of liposomes composed of photopolymerized lipids [1,2-bis-(tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine] associated with amino acids as vectors for gene delivery into the lungs of healthy animals. Lipopolymer vesicles, in particular, are more stable than other types of liposomes. In this study, lipopolymers were associated with L-arginine, L-tryptophan, or L-cysteine. We hypothesized that the addition of these amino acids would enhance the efficacy of gene delivery to the lungs by the lipopolymers. L-Arginine showed the highest association efficiency due to its positive charge and better surface interactions. None of the formulations caused inflammation or altered lung mechanics, suggesting that these lipopolymers can be safely administered as aerosols. All formulations were able to induce eGFP mRNA expression in lung tissue, but the addition of amino acids reduced delivery efficacy when compared with the simple lipopolymer particle. These results indicate that this system could be further explored for gene or drug delivery targeting lung diseases.
publishDate 2016
dc.date.none.fl_str_mv 2016-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54323
Bandeira, Elga; Lopes Pacheco, Miquéias; Chiaramoni, Nadia Silvia; Ferreira, Débora; Fernandez Ruocco, Maria Julieta; et al.; Association with amino acids does not enhance efficacy of polymerized liposomes as a system for lung gene delivery; Frontiers Research Foundation; Frontiers in Physiology; 7; 151; 4-2016; 1-9
1664-042X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54323
identifier_str_mv Bandeira, Elga; Lopes Pacheco, Miquéias; Chiaramoni, Nadia Silvia; Ferreira, Débora; Fernandez Ruocco, Maria Julieta; et al.; Association with amino acids does not enhance efficacy of polymerized liposomes as a system for lung gene delivery; Frontiers Research Foundation; Frontiers in Physiology; 7; 151; 4-2016; 1-9
1664-042X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fphys.2016.00151
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fphys.2016.00151/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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